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The map label for this gene is kinE [H]

Identifier: 159900772

GI number: 159900772

Start: 5433048

End: 5435462

Strand: Reverse

Name: kinE [H]

Synonym: Haur_4259

Alternate gene names: 159900772

Gene position: 5435462-5433048 (Counterclockwise)

Preceding gene: 159900773

Following gene: 159900767

Centisome position: 85.64

GC content: 51.06

Gene sequence:

>2415_bases
ATGGAAGAAATCCTCATCATCGACGATAGCCGTCAGATAGCCGATTTTTTGGCTGAGACGGTGCTGCCATACTATGGCTA
TCGTTCGCGCGTCGTACCCACAGGCTGGGAAGGTCTGGCTTTTTTGAAACAACGCCAGCCCGACCTAATTTTGCTTGATC
TCCAACTCCCCGACACCTCGGGGCTTGATGTGCTTCGCCAAATGGGCGAACTTGGCTACGATATTCCGGTGATTTTGATG
ACCGCCCATGGTACCGAGCAAACCGCTGTCGAGGCCTTTCGACTTGGTGCCAAAAATTATTTGATCAAGCCTTTTGATGC
TTCGGAAGCGGGCGCAGCAATTGAACGTGCCCTGCGCGAACGCCGTTTGCAGCGCGAAAAAGAGGTGCTTACCCGCACCT
TGCAGCAACGCTTGCAGGAGTTGACGATTCTCTCCAGCGTTGGTAAATCGGTGACCTCGCTGCTTGATCTCGAAGAATTG
CTCGAACGGATCGTCGATGCTGGGGTGTATCTGACCCACGCCGAAGAAGGCTATTTGCTGTTGCGCGATGGTGATGAATT
ATATTTGCGGGCGGCTAAAAATCTCGGCGAGGAGCGTGTGCAGCGTTTTCGGGTCAAGATGGATGACAATGTGGCAGGCC
AAGTTATTCGCACCGTCAAGCCAATTCGGCTTGATCGCTCGCAGCACCAAGATTTAAAACTGCGCACTGGGCTTTTGGTG
CAGGCAATGTTGCAAGTTCCCTTGATTGTTGGTCGCCATGCGATTGGTGTATTGGCGGTTGATAATCGGGTGCAGCAACG
TACCTTCAGCGAAAACGACCAATATTTGCTCTCAGCGATTGCCGATTATGCAGCGATTGCGATTGAAAATTCGCGCTTGT
TTAAATCGACCCGCGATTCTGAGCAACGCTACCGCGAATTATTCGATAACGCCAACGATATGATCTTCACGCTTGATCCA
CAGCTACGAATTGCCTCGATCAATCGTCAAGGGGTCAAATTGTTGGGCATGACCGTGGCTGAAATGATGCAGCGCACCTT
GATTTCGCTGTGTGTGCCCGACGATCAAGCAGCAATCGAACATCAATTGCAACGCCAACTAGCCAAAGCTGCTGGTGATG
GCGGTGCGTTTCCGTTGACTTTGCGTCGTGCCAATGGCGAAGATTTGCATATTGAAGTCAGCGCCCAATTGATGCAGCGT
GGCGATCAGGTAATTGGTCTGCACTGTATTGCGCGTGATGTGACCGACCGCCGCCGCTTGGAATTGCAACTATTGCAGGC
CGAAAAACTCTCAGCGATTGGCCAATTGGTGGCTGGGGTTGCTCACGAGTTGAACAATCCGATGACCAGCATCAAAGGCT
TTGCTGAGCTATTGCTGCGCCGCAAAGATTTAGATGATGATGCCCGTACCGATCTGAATTACATTAATAACCAAGCTGAA
CGAGCAGCGCGAATTGTGACCAACCTGCTGACGTTTGCCCGTGAGCATCAGCCGCAGCGGGTTTTAGTCGATGTCAATAA
AGTTATCGACGATACGCTGAGCTTGCATAGCTATCACTTGCGCGTCGATAACATCAAAGTTCAACGCCAATTCGAGCCAG
AGCTGCCAACCACCGTCGCTGATCCGTATCAATTACAACAGGTTTTTCTGAACTTGATTGGCAATGCCCATCAAGCTATG
GCCGAAAAAGGCGGCGGCGGTTTCTTGACCGTCAAAACCGAGCGGGTTGATGATGAAATTCGGATTAACATCGGCGATAC
TGGCCCCGGTATTCCCCAACACCTCGTCGGGCGGATCTTCGATCCATTTTTTACCACCAAGCCTGTTGGCAAGGGTACAG
GGCTAGGTTTATCGATCTGCTACAACATTCTGCATGATCATGGTGGCAATATTTGGGTTGATAGCGTGGCCAACGAAGGC
ACAACCTTCCATTTGGCCTTGCCGGTGGTTCAGGGCGATAATCCTGAGTTGATCAACGACGATGATCGTGAAACCACGAT
TAAGCCCGACCAAGCCTATAAAATTTTGGTGGTTGACGATGAAGAAGGCGTGGCCCAAGTGATTCAGCGCTTGGTGCGCG
ATTTGGGTCATCAGCCAGTGGTGGTGGCTAGTGGCGAGGCCGCCTTGCAAGCCGTTGATCAAGCGCCCTTCGATTTGATT
CTGAGCGATGTTAAAATGCCAGGCATGAACGGCTTCCAGCTGTATCGAGCTTTGCAACAAAAAGCCCCTGAATTGGCCAA
ACATTTTATCTTTATCACTGGCGATACGATGAGTCCAGCCACGATCACCGCGATGCGCCAAATTGGCACACCAATGATTG
CCAAGCCCTTCTCAGCCAAAAAACTCGAACGTACAATCAACGAGTTTATGGCTCGCGAAGAAGCATTGCAACGCGAGGCC
GAAATTCAGCAATAG

Upstream 100 bases:

>100_bases
TGCGGTTAAACCTATTGCGTAGAGTGATGCGACTCGTTAGAATAGCTATGAGTCCAACAATCTGCCCATACAACACGCAA
CAACACGCGAAGGATGTCCC

Downstream 100 bases:

>100_bases
TTGAGGTTGGGGGATCGGGGATTCGGTTATTGGGGATCGGGCTGCATGGCCTGATCCTCTTTTGATCCACGAAGAGCACG
AAGGTACAGGAAGGGCAATT

Product: multi-sensor hybrid histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MEEILIIDDSRQIADFLAETVLPYYGYRSRVVPTGWEGLAFLKQRQPDLILLDLQLPDTSGLDVLRQMGELGYDIPVILM
TAHGTEQTAVEAFRLGAKNYLIKPFDASEAGAAIERALRERRLQREKEVLTRTLQQRLQELTILSSVGKSVTSLLDLEEL
LERIVDAGVYLTHAEEGYLLLRDGDELYLRAAKNLGEERVQRFRVKMDDNVAGQVIRTVKPIRLDRSQHQDLKLRTGLLV
QAMLQVPLIVGRHAIGVLAVDNRVQQRTFSENDQYLLSAIADYAAIAIENSRLFKSTRDSEQRYRELFDNANDMIFTLDP
QLRIASINRQGVKLLGMTVAEMMQRTLISLCVPDDQAAIEHQLQRQLAKAAGDGGAFPLTLRRANGEDLHIEVSAQLMQR
GDQVIGLHCIARDVTDRRRLELQLLQAEKLSAIGQLVAGVAHELNNPMTSIKGFAELLLRRKDLDDDARTDLNYINNQAE
RAARIVTNLLTFAREHQPQRVLVDVNKVIDDTLSLHSYHLRVDNIKVQRQFEPELPTTVADPYQLQQVFLNLIGNAHQAM
AEKGGGGFLTVKTERVDDEIRINIGDTGPGIPQHLVGRIFDPFFTTKPVGKGTGLGLSICYNILHDHGGNIWVDSVANEG
TTFHLALPVVQGDNPELINDDDRETTIKPDQAYKILVVDDEEGVAQVIQRLVRDLGHQPVVVASGEAALQAVDQAPFDLI
LSDVKMPGMNGFQLYRALQQKAPELAKHFIFITGDTMSPATITAMRQIGTPMIAKPFSAKKLERTINEFMAREEALQREA
EIQQ

Sequences:

>Translated_804_residues
MEEILIIDDSRQIADFLAETVLPYYGYRSRVVPTGWEGLAFLKQRQPDLILLDLQLPDTSGLDVLRQMGELGYDIPVILM
TAHGTEQTAVEAFRLGAKNYLIKPFDASEAGAAIERALRERRLQREKEVLTRTLQQRLQELTILSSVGKSVTSLLDLEEL
LERIVDAGVYLTHAEEGYLLLRDGDELYLRAAKNLGEERVQRFRVKMDDNVAGQVIRTVKPIRLDRSQHQDLKLRTGLLV
QAMLQVPLIVGRHAIGVLAVDNRVQQRTFSENDQYLLSAIADYAAIAIENSRLFKSTRDSEQRYRELFDNANDMIFTLDP
QLRIASINRQGVKLLGMTVAEMMQRTLISLCVPDDQAAIEHQLQRQLAKAAGDGGAFPLTLRRANGEDLHIEVSAQLMQR
GDQVIGLHCIARDVTDRRRLELQLLQAEKLSAIGQLVAGVAHELNNPMTSIKGFAELLLRRKDLDDDARTDLNYINNQAE
RAARIVTNLLTFAREHQPQRVLVDVNKVIDDTLSLHSYHLRVDNIKVQRQFEPELPTTVADPYQLQQVFLNLIGNAHQAM
AEKGGGGFLTVKTERVDDEIRINIGDTGPGIPQHLVGRIFDPFFTTKPVGKGTGLGLSICYNILHDHGGNIWVDSVANEG
TTFHLALPVVQGDNPELINDDDRETTIKPDQAYKILVVDDEEGVAQVIQRLVRDLGHQPVVVASGEAALQAVDQAPFDLI
LSDVKMPGMNGFQLYRALQQKAPELAKHFIFITGDTMSPATITAMRQIGTPMIAKPFSAKKLERTINEFMAREEALQREA
EIQQ
>Mature_804_residues
MEEILIIDDSRQIADFLAETVLPYYGYRSRVVPTGWEGLAFLKQRQPDLILLDLQLPDTSGLDVLRQMGELGYDIPVILM
TAHGTEQTAVEAFRLGAKNYLIKPFDASEAGAAIERALRERRLQREKEVLTRTLQQRLQELTILSSVGKSVTSLLDLEEL
LERIVDAGVYLTHAEEGYLLLRDGDELYLRAAKNLGEERVQRFRVKMDDNVAGQVIRTVKPIRLDRSQHQDLKLRTGLLV
QAMLQVPLIVGRHAIGVLAVDNRVQQRTFSENDQYLLSAIADYAAIAIENSRLFKSTRDSEQRYRELFDNANDMIFTLDP
QLRIASINRQGVKLLGMTVAEMMQRTLISLCVPDDQAAIEHQLQRQLAKAAGDGGAFPLTLRRANGEDLHIEVSAQLMQR
GDQVIGLHCIARDVTDRRRLELQLLQAEKLSAIGQLVAGVAHELNNPMTSIKGFAELLLRRKDLDDDARTDLNYINNQAE
RAARIVTNLLTFAREHQPQRVLVDVNKVIDDTLSLHSYHLRVDNIKVQRQFEPELPTTVADPYQLQQVFLNLIGNAHQAM
AEKGGGGFLTVKTERVDDEIRINIGDTGPGIPQHLVGRIFDPFFTTKPVGKGTGLGLSICYNILHDHGGNIWVDSVANEG
TTFHLALPVVQGDNPELINDDDRETTIKPDQAYKILVVDDEEGVAQVIQRLVRDLGHQPVVVASGEAALQAVDQAPFDLI
LSDVKMPGMNGFQLYRALQQKAPELAKHFIFITGDTMSPATITAMRQIGTPMIAKPFSAKKLERTINEFMAREEALQREA
EIQQ

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=36.5853658536585, Blast_Score=147, Evalue=4e-36,
Organism=Escherichia coli, GI1788549, Length=278, Percent_Identity=35.2517985611511, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1790300, Length=272, Percent_Identity=35.6617647058824, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI48994928, Length=358, Percent_Identity=27.6536312849162, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI87081816, Length=331, Percent_Identity=26.8882175226586, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788713, Length=359, Percent_Identity=27.2980501392758, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI145693157, Length=277, Percent_Identity=29.2418772563177, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788550, Length=143, Percent_Identity=34.2657342657343, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=238, Percent_Identity=28.1512605042017, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1790299, Length=123, Percent_Identity=35.7723577235772, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786600, Length=283, Percent_Identity=27.5618374558304, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788394, Length=123, Percent_Identity=34.1463414634146, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=32.4786324786325, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=37.8151260504202, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=233, Percent_Identity=27.8969957081545, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=238, Percent_Identity=24.3697478991597, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=27.1929824561404, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1790346, Length=208, Percent_Identity=25.9615384615385, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789402, Length=126, Percent_Identity=35.7142857142857, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=32.2033898305085, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1789808, Length=217, Percent_Identity=23.5023041474654, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 90049; Mature: 90049

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEILIIDDSRQIADFLAETVLPYYGYRSRVVPTGWEGLAFLKQRQPDLILLDLQLPDTS
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCC
GLDVLRQMGELGYDIPVILMTAHGTEQTAVEAFRLGAKNYLIKPFDASEAGAAIERALRE
CHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHH
RRLQREKEVLTRTLQQRLQELTILSSVGKSVTSLLDLEELLERIVDAGVYLTHAEEGYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
LRDGDELYLRAAKNLGEERVQRFRVKMDDNVAGQVIRTVKPIRLDRSQHQDLKLRTGLLV
EECCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
QAMLQVPLIVGRHAIGVLAVDNRVQQRTFSENDQYLLSAIADYAAIAIENSRLFKSTRDS
HHHHHHHHHHCCHHEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHEEEECCHHHHHCCHH
EQRYRELFDNANDMIFTLDPQLRIASINRQGVKLLGMTVAEMMQRTLISLCVPDDQAAIE
HHHHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
HQLQRQLAKAAGDGGAFPLTLRRANGEDLHIEVSAQLMQRGDQVIGLHCIARDVTDRRRL
HHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEHHHHHCCCCEEEHHHHHHHHHHHHHH
ELQLLQAEKLSAIGQLVAGVAHELNNPMTSIKGFAELLLRRKDLDDDARTDLNYINNQAE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RAARIVTNLLTFAREHQPQRVLVDVNKVIDDTLSLHSYHLRVDNIKVQRQFEPELPTTVA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHEEEEEECCEEEEEECCCCCCCCCC
DPYQLQQVFLNLIGNAHQAMAEKGGGGFLTVKTERVDDEIRINIGDTGPGIPQHLVGRIF
CHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHH
DPFFTTKPVGKGTGLGLSICYNILHDHGGNIWVDSVANEGTTFHLALPVVQGDNPELIND
CCHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCC
DDRETTIKPDQAYKILVVDDEEGVAQVIQRLVRDLGHQPVVVASGEAALQAVDQAPFDLI
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHH
LSDVKMPGMNGFQLYRALQQKAPELAKHFIFITGDTMSPATITAMRQIGTPMIAKPFSAK
HHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHH
KLERTINEFMAREEALQREAEIQQ
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEEILIIDDSRQIADFLAETVLPYYGYRSRVVPTGWEGLAFLKQRQPDLILLDLQLPDTS
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCC
GLDVLRQMGELGYDIPVILMTAHGTEQTAVEAFRLGAKNYLIKPFDASEAGAAIERALRE
CHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHH
RRLQREKEVLTRTLQQRLQELTILSSVGKSVTSLLDLEELLERIVDAGVYLTHAEEGYLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
LRDGDELYLRAAKNLGEERVQRFRVKMDDNVAGQVIRTVKPIRLDRSQHQDLKLRTGLLV
EECCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
QAMLQVPLIVGRHAIGVLAVDNRVQQRTFSENDQYLLSAIADYAAIAIENSRLFKSTRDS
HHHHHHHHHHCCHHEEEEEECCHHHHHHCCCCHHHHHHHHHHHHHEEEECCHHHHHCCHH
EQRYRELFDNANDMIFTLDPQLRIASINRQGVKLLGMTVAEMMQRTLISLCVPDDQAAIE
HHHHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
HQLQRQLAKAAGDGGAFPLTLRRANGEDLHIEVSAQLMQRGDQVIGLHCIARDVTDRRRL
HHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEHHHHHCCCCEEEHHHHHHHHHHHHHH
ELQLLQAEKLSAIGQLVAGVAHELNNPMTSIKGFAELLLRRKDLDDDARTDLNYINNQAE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RAARIVTNLLTFAREHQPQRVLVDVNKVIDDTLSLHSYHLRVDNIKVQRQFEPELPTTVA
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHEEEEEECCEEEEEECCCCCCCCCC
DPYQLQQVFLNLIGNAHQAMAEKGGGGFLTVKTERVDDEIRINIGDTGPGIPQHLVGRIF
CHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHH
DPFFTTKPVGKGTGLGLSICYNILHDHGGNIWVDSVANEGTTFHLALPVVQGDNPELIND
CCHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCC
DDRETTIKPDQAYKILVVDDEEGVAQVIQRLVRDLGHQPVVVASGEAALQAVDQAPFDLI
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHH
LSDVKMPGMNGFQLYRALQQKAPELAKHFIFITGDTMSPATITAMRQIGTPMIAKPFSAK
HHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHH
KLERTINEFMAREEALQREAEIQQ
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]