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The map label for this gene is mdh [H]

Identifier: 159900719

GI number: 159900719

Start: 5359688

End: 5360632

Strand: Reverse

Name: mdh [H]

Synonym: Haur_4206

Alternate gene names: 159900719

Gene position: 5360632-5359688 (Counterclockwise)

Preceding gene: 159900720

Following gene: 159900718

Centisome position: 84.46

GC content: 49.42

Gene sequence:

>945_bases
GTGGCTAATCGTAAGAAAGTAACCATCATCGGGGCTGGATTTGTTGGCTCAACCTGTGCACACTGGTTGGCAAGCAAAGA
ACTCGCCGATGTTGTTTTAGTCGATATTGTTGAGGGCATTCCTCAAGGCAAAGGCTTGGATTTACTACAATCAGGGCCAG
TTGAAGGCTTTGATGTCAGCGTGATCGGCACCAACAGCTATGAAGAAACCACCGATTCAGATGTGGTAATTTTGACCTCA
GGTGCGCCACGCAAACCAGGCATGACCCGCGAAGATTTGCTCAAAATCAACGCTGAAATTACCAAATCAAACATCGAAAA
GGTTGCCAAAACCTCGCCTAACGCTTGTATCATTGTGGTCAACAACCCAATGGACACGATGACCTACCTCGCTCGCGTGG
CTTCAGGCTTCCCCAAAGAGCGCGTGATGGGTCAAGGTGGCGTGTTGGATGCAGCTCGCTATCGCACTTTCTTGGCGCAA
GAATTGAATGTTTCAGTTGAAGATATTCAAGCCATGTTGATGGGCGGCCACGGCGATGAAATGGTTCCATTGCCACGCTA
CACCACGGTTTCAGGGATTCCTGTAACCGAATTTATCAGCGCTGAACGCTTGAACCAAATCGTCGAACGCACCAAAAAGG
GTGGCGGCGAAATCGTTTCATTGCTCAAAACTGGCTCAGCCTACTATGCTCCTGCCGCTGCTACCATCCAAATGGTTGAA
GCGATCTTGAAGGATAAGAAGCGTGTTCTGCCAGCCGCTGCTTATCTCGAAGGCGAATATGGCATTAACGATCTCTACTT
CGGCGTGCCAGTTGTTTTGGGCGCTGGTGGTGTTGAAAGAATTCTCGAATTGCCATTGAGCGACGACGAAAAGGCCTTGA
TGGCCAAATCAGCCGACTTGGTTCGCAGCTCAGTCGATACCTTACGCACCTTGATCGATTTCTAA

Upstream 100 bases:

>100_bases
AGGGAACTTATTACGGTTAAGGCGCAAGCGTGAGAGATGGGTCGTAGACGCATCTCCGACACTTGAATCGTAACCACAGC
TTGATATTTTGGAGGCATGT

Downstream 100 bases:

>100_bases
TTGATCGTTTGGTTGCAAGCAGCGCTGTGGTTTTTGGCCACAGCGCCCTTTTTTAAACAATGACGCTTGAAACTGCCCAA
AATGCTCGTTTGCGTGGCGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MANRKKVTIIGAGFVGSTCAHWLASKELADVVLVDIVEGIPQGKGLDLLQSGPVEGFDVSVIGTNSYEETTDSDVVILTS
GAPRKPGMTREDLLKINAEITKSNIEKVAKTSPNACIIVVNNPMDTMTYLARVASGFPKERVMGQGGVLDAARYRTFLAQ
ELNVSVEDIQAMLMGGHGDEMVPLPRYTTVSGIPVTEFISAERLNQIVERTKKGGGEIVSLLKTGSAYYAPAAATIQMVE
AILKDKKRVLPAAAYLEGEYGINDLYFGVPVVLGAGGVERILELPLSDDEKALMAKSADLVRSSVDTLRTLIDF

Sequences:

>Translated_314_residues
MANRKKVTIIGAGFVGSTCAHWLASKELADVVLVDIVEGIPQGKGLDLLQSGPVEGFDVSVIGTNSYEETTDSDVVILTS
GAPRKPGMTREDLLKINAEITKSNIEKVAKTSPNACIIVVNNPMDTMTYLARVASGFPKERVMGQGGVLDAARYRTFLAQ
ELNVSVEDIQAMLMGGHGDEMVPLPRYTTVSGIPVTEFISAERLNQIVERTKKGGGEIVSLLKTGSAYYAPAAATIQMVE
AILKDKKRVLPAAAYLEGEYGINDLYFGVPVVLGAGGVERILELPLSDDEKALMAKSADLVRSSVDTLRTLIDF
>Mature_313_residues
ANRKKVTIIGAGFVGSTCAHWLASKELADVVLVDIVEGIPQGKGLDLLQSGPVEGFDVSVIGTNSYEETTDSDVVILTSG
APRKPGMTREDLLKINAEITKSNIEKVAKTSPNACIIVVNNPMDTMTYLARVASGFPKERVMGQGGVLDAARYRTFLAQE
LNVSVEDIQAMLMGGHGDEMVPLPRYTTVSGIPVTEFISAERLNQIVERTKKGGGEIVSLLKTGSAYYAPAAATIQMVEA
ILKDKKRVLPAAAYLEGEYGINDLYFGVPVVLGAGGVERILELPLSDDEKALMAKSADLVRSSVDTLRTLIDF

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=306, Percent_Identity=33.6601307189542, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI221136809, Length=306, Percent_Identity=33.6601307189542, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=32.7868852459016, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=32.7868852459016, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=31.4754098360656, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI291575128, Length=317, Percent_Identity=30.9148264984227, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4557032, Length=317, Percent_Identity=30.9148264984227, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI9257228, Length=303, Percent_Identity=33.6633663366337, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4504973, Length=303, Percent_Identity=33.6633663366337, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI260099727, Length=191, Percent_Identity=37.696335078534, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI260099725, Length=191, Percent_Identity=37.696335078534, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI207028494, Length=189, Percent_Identity=30.1587301587302, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI103472011, Length=288, Percent_Identity=26.0416666666667, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI21735621, Length=256, Percent_Identity=28.515625, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789632, Length=290, Percent_Identity=30, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=290, Percent_Identity=31.3793103448276, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17554310, Length=225, Percent_Identity=31.5555555555556, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=303, Percent_Identity=30.3630363036304, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=308, Percent_Identity=29.5454545454545, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17136226, Length=306, Percent_Identity=29.7385620915033, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45550422, Length=308, Percent_Identity=28.8961038961039, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24647881, Length=307, Percent_Identity=30.2931596091205, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24663599, Length=239, Percent_Identity=28.4518828451883, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663595, Length=267, Percent_Identity=26.9662921348315, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33635; Mature: 33504

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRKKVTIIGAGFVGSTCAHWLASKELADVVLVDIVEGIPQGKGLDLLQSGPVEGFDVS
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEE
VIGTNSYEETTDSDVVILTSGAPRKPGMTREDLLKINAEITKSNIEKVAKTSPNACIIVV
EEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
NNPMDTMTYLARVASGFPKERVMGQGGVLDAARYRTFLAQELNVSVEDIQAMLMGGHGDE
CCCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
MVPLPRYTTVSGIPVTEFISAERLNQIVERTKKGGGEIVSLLKTGSAYYAPAAATIQMVE
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHH
AILKDKKRVLPAAAYLEGEYGINDLYFGVPVVLGAGGVERILELPLSDDEKALMAKSADL
HHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHH
VRSSVDTLRTLIDF
HHHHHHHHHHHHCC
>Mature Secondary Structure 
ANRKKVTIIGAGFVGSTCAHWLASKELADVVLVDIVEGIPQGKGLDLLQSGPVEGFDVS
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEE
VIGTNSYEETTDSDVVILTSGAPRKPGMTREDLLKINAEITKSNIEKVAKTSPNACIIVV
EEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
NNPMDTMTYLARVASGFPKERVMGQGGVLDAARYRTFLAQELNVSVEDIQAMLMGGHGDE
CCCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
MVPLPRYTTVSGIPVTEFISAERLNQIVERTKKGGGEIVSLLKTGSAYYAPAAATIQMVE
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHH
AILKDKKRVLPAAAYLEGEYGINDLYFGVPVVLGAGGVERILELPLSDDEKALMAKSADL
HHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHH
VRSSVDTLRTLIDF
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA