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The map label for this gene is pycA [H]

Identifier: 159900720

GI number: 159900720

Start: 5360845

End: 5362611

Strand: Reverse

Name: pycA [H]

Synonym: Haur_4207

Alternate gene names: 159900720

Gene position: 5362611-5360845 (Counterclockwise)

Preceding gene: 159900721

Following gene: 159900719

Centisome position: 84.5

GC content: 53.42

Gene sequence:

>1767_bases
ATGAGTTTCGATACAGTGCTGATCGCCAATCGCGGCGAGATTGCCCTGCGCGTGATGCGGGCATGCAAGGAATTAGGTTT
ACGCACCGTCGCCGTCTACTCAGAAGCGGATCGCGATTCGCTCCACGTCCGTTATGCCGACGATGCCTTTTTGATCGGTC
CCCCTCCAGCAGTCCAAAGTTATTTGCAAACTGAAACAATCCTCGATGTTGCGCGGCGCAGTGGCGCTGGCGCGATTCAC
CCTGGCTACGGCTTCCTCTCGGAAAATACCGATTTTGTGCGCACTTGTGATGCGGCGGGCATTGCCTTTATTGGGCCAAC
CGCTGATGCCATGGATTTGATGGGCGGTAAAATTCACGCTCGTCAAGTGGCTTTACGTGCTCATGTCCCGCTGGTTCCAG
GCACAACCGAGGCGGTTGAAAGTGTCGCCGAAGCCCTTGAACTTGGCGAACAATATGGCTACCCAATTGCCATCAAAGCT
AGTGCTGGCGGCGGTGGCCGTGGCTTGAAGGTGGCCTACCAGCCTGAAGAAGTTGAATTTGCCTTCGAAAGTGCACGTCG
CGAGGCCGAAGCGGCTTTCAAAAACGGCGAATTGTTTGTTGAAAAATATGTGCTCGATCCACGCCACATTGAAATTCAGA
TTCTGGCTGATCAATATGGCAATGTGGTGTATTTGGGCGAGCGCGATTGTTCGGTGCAGCGTCGCCACCAAAAATTGATT
GAAGAAACTCCATCGCCAGCAGTTTCGCCTGAATTGCGCCGCACCATGGGCGAATGTGCTTTGCGCTTATGCCGCGAAAC
GAGTTATGTTGGCGCTGGCACTTTGGAGTTTTTGCTAGCCCCCGATGGTCAATTCTATTTCCTCGAAATGAACACGCGGA
TTCAAGTTGAGCATACTGTGACCGAAATGGTTACGGGCATCGATTTGGTGCAAGCCCAACTCCGCATCGCCCAAGGCGAA
AAGCTTTGGTTCACCCAAGAGGAAATTCAGCTACGCGGCCATGCGATTCAATGCCGGATCAATGCTGAGGATGCGGCGGC
AGGTTTTCGCCCAGCGCTTGGCACGATCAGCGCTTACAACGAACCTAAAGGCTATGGCGTGCGGGTCGATGCTGGGGTTG
AGCAAGGCACAACCATTCCACCATACTACGATTCGATGCTTGCCAAATTGGTGACTTGGGGTGCAACTCGTGGCGAAGCC
TTGCAACGCATGCGCCGTGCCCTCAACGATTACACGATCGAAGGCATTACCACGGTGATTCCTTTCCATAAATTGGCTTT
GGCTGAGCCAGCTTTTGAGCATGGCGATGTGACGGTGAGCTTTATTCCACGCTATTTGGAAGCAAAACTCAAGCAATTGC
CAAGCGCCACGCCGAGCACTACCGAGCCAGCCGCCGAGCAACCTAGCCGCGAATTAATGGTTGAGGTCAATGGGCGACGC
TTTGCTGTGCGCGTTGCTGGCGAAGGTTTGAATACACCAATTGCTAGCAATAAAGCGGCTGCGCCAACCCGCCATCGCGC
TGCCAACAAAAAGCGTGAAGTTGCGACAGATCCCAATGCAGTGATTTGTCCAATTCAAGGTACAATTGTGGCGATTAAAA
CCAGCGTTGGCGCGGCGGTTGAGGCTGGCCAAGTGGTGTTTGTCGTCGAAGCAATGAAGATGGAGAACGAAATCGCAACT
CCACGGGCTGGTACAATTGCTACAATCAATGCCGAGGTTGGCAAAAGCATCGAGGCTGGCAGCGTGCTAGCAACCCTCGA
AGCATAA

Upstream 100 bases:

>100_bases
CGAGCAGCTGGCGGCTTTTACGGCATTACGGGACTCTAATTTCGCATACGGCAATGATTAACGTAAGACTTGCCATAGCA
CAACGGAGTTGCAACGGCAT

Downstream 100 bases:

>100_bases
ATGAGGCTATCGATAATCCCTCACCCCCAGCCCCTCTCCCACTGCGGCGGGAGAGGGGAGTTAGGTAGGTGCTTCCCCCT
CGCCTGCTGGGCGGGAGAGG

Product: acetyl-CoA carboxylase, biotin carboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSFDTVLIANRGEIALRVMRACKELGLRTVAVYSEADRDSLHVRYADDAFLIGPPPAVQSYLQTETILDVARRSGAGAIH
PGYGFLSENTDFVRTCDAAGIAFIGPTADAMDLMGGKIHARQVALRAHVPLVPGTTEAVESVAEALELGEQYGYPIAIKA
SAGGGGRGLKVAYQPEEVEFAFESARREAEAAFKNGELFVEKYVLDPRHIEIQILADQYGNVVYLGERDCSVQRRHQKLI
EETPSPAVSPELRRTMGECALRLCRETSYVGAGTLEFLLAPDGQFYFLEMNTRIQVEHTVTEMVTGIDLVQAQLRIAQGE
KLWFTQEEIQLRGHAIQCRINAEDAAAGFRPALGTISAYNEPKGYGVRVDAGVEQGTTIPPYYDSMLAKLVTWGATRGEA
LQRMRRALNDYTIEGITTVIPFHKLALAEPAFEHGDVTVSFIPRYLEAKLKQLPSATPSTTEPAAEQPSRELMVEVNGRR
FAVRVAGEGLNTPIASNKAAAPTRHRAANKKREVATDPNAVICPIQGTIVAIKTSVGAAVEAGQVVFVVEAMKMENEIAT
PRAGTIATINAEVGKSIEAGSVLATLEA

Sequences:

>Translated_588_residues
MSFDTVLIANRGEIALRVMRACKELGLRTVAVYSEADRDSLHVRYADDAFLIGPPPAVQSYLQTETILDVARRSGAGAIH
PGYGFLSENTDFVRTCDAAGIAFIGPTADAMDLMGGKIHARQVALRAHVPLVPGTTEAVESVAEALELGEQYGYPIAIKA
SAGGGGRGLKVAYQPEEVEFAFESARREAEAAFKNGELFVEKYVLDPRHIEIQILADQYGNVVYLGERDCSVQRRHQKLI
EETPSPAVSPELRRTMGECALRLCRETSYVGAGTLEFLLAPDGQFYFLEMNTRIQVEHTVTEMVTGIDLVQAQLRIAQGE
KLWFTQEEIQLRGHAIQCRINAEDAAAGFRPALGTISAYNEPKGYGVRVDAGVEQGTTIPPYYDSMLAKLVTWGATRGEA
LQRMRRALNDYTIEGITTVIPFHKLALAEPAFEHGDVTVSFIPRYLEAKLKQLPSATPSTTEPAAEQPSRELMVEVNGRR
FAVRVAGEGLNTPIASNKAAAPTRHRAANKKREVATDPNAVICPIQGTIVAIKTSVGAAVEAGQVVFVVEAMKMENEIAT
PRAGTIATINAEVGKSIEAGSVLATLEA
>Mature_587_residues
SFDTVLIANRGEIALRVMRACKELGLRTVAVYSEADRDSLHVRYADDAFLIGPPPAVQSYLQTETILDVARRSGAGAIHP
GYGFLSENTDFVRTCDAAGIAFIGPTADAMDLMGGKIHARQVALRAHVPLVPGTTEAVESVAEALELGEQYGYPIAIKAS
AGGGGRGLKVAYQPEEVEFAFESARREAEAAFKNGELFVEKYVLDPRHIEIQILADQYGNVVYLGERDCSVQRRHQKLIE
ETPSPAVSPELRRTMGECALRLCRETSYVGAGTLEFLLAPDGQFYFLEMNTRIQVEHTVTEMVTGIDLVQAQLRIAQGEK
LWFTQEEIQLRGHAIQCRINAEDAAAGFRPALGTISAYNEPKGYGVRVDAGVEQGTTIPPYYDSMLAKLVTWGATRGEAL
QRMRRALNDYTIEGITTVIPFHKLALAEPAFEHGDVTVSFIPRYLEAKLKQLPSATPSTTEPAAEQPSRELMVEVNGRRF
AVRVAGEGLNTPIASNKAAAPTRHRAANKKREVATDPNAVICPIQGTIVAIKTSVGAAVEAGQVVFVVEAMKMENEIATP
RAGTIATINAEVGKSIEAGSVLATLEA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG4770

COG function: function code I; Acetyl/propionyl-CoA carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI295821183, Length=629, Percent_Identity=39.1096979332273, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI116805327, Length=441, Percent_Identity=49.8866213151927, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI65506442, Length=443, Percent_Identity=46.27539503386, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI189095269, Length=443, Percent_Identity=46.27539503386, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI106049528, Length=442, Percent_Identity=47.737556561086, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI106049295, Length=442, Percent_Identity=47.737556561086, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI106049292, Length=442, Percent_Identity=47.737556561086, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI38679960, Length=509, Percent_Identity=32.2200392927308, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI38679974, Length=509, Percent_Identity=32.2200392927308, Blast_Score=257, Evalue=3e-68,
Organism=Homo sapiens, GI38679977, Length=509, Percent_Identity=32.2200392927308, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI38679967, Length=509, Percent_Identity=32.2200392927308, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI38679971, Length=509, Percent_Identity=32.2200392927308, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI134142062, Length=510, Percent_Identity=32.156862745098, Blast_Score=252, Evalue=7e-67,
Organism=Escherichia coli, GI1789654, Length=449, Percent_Identity=49.8886414253898, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17567343, Length=443, Percent_Identity=46.7268623024831, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71987519, Length=441, Percent_Identity=48.9795918367347, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17562816, Length=450, Percent_Identity=48.6666666666667, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71997168, Length=453, Percent_Identity=32.0088300220751, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71997163, Length=453, Percent_Identity=32.0088300220751, Blast_Score=210, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI133931226, Length=457, Percent_Identity=28.0087527352298, Blast_Score=191, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319695, Length=446, Percent_Identity=46.1883408071749, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321376, Length=446, Percent_Identity=46.8609865470852, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319685, Length=444, Percent_Identity=41.6666666666667, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323863, Length=453, Percent_Identity=32.8918322295806, Blast_Score=233, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6324343, Length=513, Percent_Identity=30.4093567251462, Blast_Score=223, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24651757, Length=439, Percent_Identity=46.9248291571754, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24652212, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652210, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652214, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI19921944, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652216, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281363050, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652224, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652222, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652220, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652218, Length=443, Percent_Identity=47.6297968397291, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651759, Length=404, Percent_Identity=45.7920792079208, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI161076409, Length=467, Percent_Identity=31.9057815845824, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI161076407, Length=467, Percent_Identity=31.9057815845824, Blast_Score=233, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24586460, Length=467, Percent_Identity=31.9057815845824, Blast_Score=233, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24586458, Length=467, Percent_Identity=31.9057815845824, Blast_Score=233, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 63711; Mature: 63580

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDTVLIANRGEIALRVMRACKELGLRTVAVYSEADRDSLHVRYADDAFLIGPPPAVQS
CCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCEEEECCCHHHHH
YLQTETILDVARRSGAGAIHPGYGFLSENTDFVRTCDAAGIAFIGPTADAMDLMGGKIHA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCHHHHHCCCEEEH
RQVALRAHVPLVPGTTEAVESVAEALELGEQYGYPIAIKASAGGGGRGLKVAYQPEEVEF
HEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHHHH
AFESARREAEAAFKNGELFVEKYVLDPRHIEIQILADQYGNVVYLGERDCSVQRRHQKLI
HHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHH
EETPSPAVSPELRRTMGECALRLCRETSYVGAGTLEFLLAPDGQFYFLEMNTRIQVEHTV
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCEEEEHHHH
TEMVTGIDLVQAQLRIAQGEKLWFTQEEIQLRGHAIQCRINAEDAAAGFRPALGTISAYN
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCEEEEEEECCHHCCCCCCCCCCCCCCCC
EPKGYGVRVDAGVEQGTTIPPYYDSMLAKLVTWGATRGEALQRMRRALNDYTIEGITTVI
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
PFHKLALAEPAFEHGDVTVSFIPRYLEAKLKQLPSATPSTTEPAAEQPSRELMVEVNGRR
CHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCE
FAVRVAGEGLNTPIASNKAAAPTRHRAANKKREVATDPNAVICPIQGTIVAIKTSVGAAV
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
EAGQVVFVVEAMKMENEIATPRAGTIATINAEVGKSIEAGSVLATLEA
CCCCEEEEEEEHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCEEEEECC
>Mature Secondary Structure 
SFDTVLIANRGEIALRVMRACKELGLRTVAVYSEADRDSLHVRYADDAFLIGPPPAVQS
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCEEEECCCHHHHH
YLQTETILDVARRSGAGAIHPGYGFLSENTDFVRTCDAAGIAFIGPTADAMDLMGGKIHA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCHHHHHCCCEEEH
RQVALRAHVPLVPGTTEAVESVAEALELGEQYGYPIAIKASAGGGGRGLKVAYQPEEVEF
HEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHHHH
AFESARREAEAAFKNGELFVEKYVLDPRHIEIQILADQYGNVVYLGERDCSVQRRHQKLI
HHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHH
EETPSPAVSPELRRTMGECALRLCRETSYVGAGTLEFLLAPDGQFYFLEMNTRIQVEHTV
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCEEEEHHHH
TEMVTGIDLVQAQLRIAQGEKLWFTQEEIQLRGHAIQCRINAEDAAAGFRPALGTISAYN
HHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCEEEEEEECCHHCCCCCCCCCCCCCCCC
EPKGYGVRVDAGVEQGTTIPPYYDSMLAKLVTWGATRGEALQRMRRALNDYTIEGITTVI
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
PFHKLALAEPAFEHGDVTVSFIPRYLEAKLKQLPSATPSTTEPAAEQPSRELMVEVNGRR
CHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCE
FAVRVAGEGLNTPIASNKAAAPTRHRAANKKREVATDPNAVICPIQGTIVAIKTSVGAAV
EEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
EAGQVVFVVEAMKMENEIATPRAGTIATINAEVGKSIEAGSVLATLEA
CCCCEEEEEEEHHHHHHCCCCCCCEEEEEEHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]