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The map label for this gene is queD [H]

Identifier: 159900636

GI number: 159900636

Start: 5271219

End: 5271620

Strand: Direct

Name: queD [H]

Synonym: Haur_4123

Alternate gene names: 159900636

Gene position: 5271219-5271620 (Clockwise)

Preceding gene: 159900635

Following gene: 159900637

Centisome position: 83.06

GC content: 48.76

Gene sequence:

>402_bases
ATGTACGCGATTCTTACCAAACAATTTCGCTTCGAAGCAGCCCATCAACTCCCCAATCATCGCGGCAAATGTGCGCGGTT
ACATGGCCATTCCTATTTATTAGAGGTCAGTGTGCGTGGGCTGATTCAGCCCACTCGTGGTCAAAGCGATGATGGCATGG
TGATTGATTTAGAACAAATTAAGCAGTTGGTCAACGAAATTATTATTGCTCGCGTTGATCACTATAATCTTAACGATTTT
TTGAGTGTGCCCAGCACCGCCGAAAATATCGCCCATTGGATGTGGGATCAGCTTGAACAGCAAGCGCCTGAATTTGCCGC
CTTGCTATGGAGAATCCGGCTGTGGGAGACTGCCAGTGGCTATGTTGAAATTAGCCGTGCCGAACGCGAGGCCCAGCCAT
GA

Upstream 100 bases:

>100_bases
TTCTTTGCTCTATGTTCTACTTGCCCCGTCCCCATGGTACAATAGCCATGTGCGTCAACCGTTGGCGCTTTTTTTGACAT
TTAAACACCTAGTAGATGAT

Downstream 100 bases:

>100_bases
ATGTGATGGAAGTTTATCGCTCAGTCCAAGGTGAAGGCACCTTGATGGGTGTGCCAACCACGTTTGTACGCTTTTTTGCC
TGCAATTTGCGCTGTAGCTG

Product: putative 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MYAILTKQFRFEAAHQLPNHRGKCARLHGHSYLLEVSVRGLIQPTRGQSDDGMVIDLEQIKQLVNEIIIARVDHYNLNDF
LSVPSTAENIAHWMWDQLEQQAPEFAALLWRIRLWETASGYVEISRAEREAQP

Sequences:

>Translated_133_residues
MYAILTKQFRFEAAHQLPNHRGKCARLHGHSYLLEVSVRGLIQPTRGQSDDGMVIDLEQIKQLVNEIIIARVDHYNLNDF
LSVPSTAENIAHWMWDQLEQQAPEFAALLWRIRLWETASGYVEISRAEREAQP
>Mature_133_residues
MYAILTKQFRFEAAHQLPNHRGKCARLHGHSYLLEVSVRGLIQPTRGQSDDGMVIDLEQIKQLVNEIIIARVDHYNLNDF
LSVPSTAENIAHWMWDQLEQQAPEFAALLWRIRLWETASGYVEISRAEREAQP

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506331, Length=136, Percent_Identity=31.6176470588235, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789124, Length=112, Percent_Identity=35.7142857142857, Blast_Score=73, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19550074, Length=138, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585378, Length=138, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 15409; Mature: 15409

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAILTKQFRFEAAHQLPNHRGKCARLHGHSYLLEVSVRGLIQPTRGQSDDGMVIDLEQI
CCEEHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEEHHHCCCCCCCCCCCCEEEEHHHH
KQLVNEIIIARVDHYNLNDFLSVPSTAENIAHWMWDQLEQQAPEFAALLWRIRLWETASG
HHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
YVEISRAEREAQP
EEEEECCCCCCCC
>Mature Secondary Structure
MYAILTKQFRFEAAHQLPNHRGKCARLHGHSYLLEVSVRGLIQPTRGQSDDGMVIDLEQI
CCEEHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEEHHHCCCCCCCCCCCCEEEEHHHH
KQLVNEIIIARVDHYNLNDFLSVPSTAENIAHWMWDQLEQQAPEFAALLWRIRLWETASG
HHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
YVEISRAEREAQP
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]