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The map label for this gene is priA [H]

Identifier: 159900617

GI number: 159900617

Start: 5237326

End: 5239815

Strand: Reverse

Name: priA [H]

Synonym: Haur_4104

Alternate gene names: 159900617

Gene position: 5239815-5237326 (Counterclockwise)

Preceding gene: 159900619

Following gene: 159900615

Centisome position: 82.56

GC content: 55.3

Gene sequence:

>2490_bases
ATGTATGCCCAGGTTGCGCTCAATGCCTATTTGCCCGATAACCCCGAGGCGATTTTGACCTATGCCGTGCCGCCGCATCT
CCGACCTTTGCGTGTAGGTGCGTTGGTGTGGGTTCCGCTTGGGCCGCGTCGCGTGCAGGGCGTGGTTTTAGAATTTAGCA
CTCAACGGCCTCAAGGTTTTGTGGTGCGCGATATTTTAGAACACGCCGACCCCGAAGCGATTGCCGCCCCAGCCCAATTG
GCCTTGGCGCAGTGGTTTAGCCAATCTCAAGCCGTGCGTTTGTGGGACGCTGTGCAATTGACCATGCCGCCAGGGGTGAA
GCAAGAAATTGAGCGCACTTGGCGGCCAACTGAGCATGGCCAGAAGATCGAACTTGGCACGCTCGACGAACGCGAACGAG
CAATTTTGTTTCATTTACGGCGGGTTGGCGAGCAAAGCGAACGGCAGCTACGGGTCGATTTACGTGGCTCAGACGCTGAT
CTGCGGCGTAGCTATACCGAATTGCATGAACGTGGATTGATCGCCTTGGGTAGTTTGGTTTCGCGACCAGCGGCCAAGCC
CAAACGTGAATGGAGTGCCCAGCCAACCAGCGCCACTCCAGCCGAAGCCTTGGCTGAGTTGAGCAAAGCGCCACGCTTGG
CGGCAATTTTTCAGGCGATTTTAGAGCAAGCAGTAGATGGCGAACCAGTGCTATTGCCTGAGTTGCGTGATGTTGGCGCA
AGCTTGAGCCATTTGCGTAGTTTGGCCTTGCGCAATTTGATTCAGCTGGAGCAAATTGAGGTGCGCCGTGACCCGCTGCT
TGGGCGCATCCCACCCTTGGAGTTTCCGCCCGAACTAACGCTTGAGCAAGAACGTGGTTGGCGGCAGATTGTCGCGGCCC
AAGATACAGCAAGTCGCACAGGCAAGGCTAAACCAATTTTGCTGCATGGCGTGACGGGTAGCGGCAAAACCGAGCTATAT
CTACGGGCAATTGGCCGAGCCTTACGCAATGGTGGCCAAGCCTTGGTGCTCGTGCCCGAAATCGCCCTGACAACCCAGCT
GGTGCGCCGTTTTGCTGCCCGCTTTCCGCAACAATTGGCGGTGTTGCACTCCGAATTGAGCGTTGGCGAGCGCTACGATG
AATGGCGGCGTTTGCGGCGTGGCGATGCCCAAGTGGCCATTGGCTCGCGTTCAGCAATTTGGTCGCCCATGAGCAAATTG
CAGCTAATTATTGTTGATGAAGAACATGAACATTCCTTCAAGCACGAAGCCACGCCCTACTACCACGCCCGTGATTTGGC
AGTGAAATTGGCCGAATTGGCTGGAGCCGTGTTGATTTTGGGCAGCGCCACACCCTCGCTAGAAACAGCCTACGCCGTGC
AGATCAACAAATATGAAGTGTTGGAATTGCACGAACGGGTTGGGCGCAAAACGGTGGCGGGGGTCACCAAAGCAGATCCC
ATCCCCATGCCGCCAGTACAAATTGTGGATATGCGCAGCGAACTCAAGGCGGGCGTGCGTTCGATGTTTAGTCGTCCCTT
GCAAAGCGCGATTCAACGCACCCTCGATAAGCGCGAACAAACGATTTTATTTTTGAATCGACGCGGGATTGCCTCGTTTG
TGATGTGTCGCGATTGTGGCACGACGATTCAATGCCCACGCTGTGCCGTAGCCTTGGCGGTGCACGCAACTGCGGGCGAC
GCGGCTGCGCCAGCAACCCTGCGCTGCCATTATTGTGGCCATGTTGATTGGGTTCCGCGTAATTGCCCAAGCTGTTTCAG
CACCCGCATTCGTGATTTTGGGGTTGGTACGCAGCGGGTGGTCGAAGAAGTCAAATTGCTCTATCCCCAAGCACGGGTGA
TGCGCTGGGATCGCGATGTGACTGGGCGTAAAGGCTCGCACGAAGCGTTGCTGAACCAATTTACCCAACACGAAGCCGAT
ATTTTGGTGGGAACTCAGATGGTTGCCAAGGGCTTTGATTTGCCGTTGGTGACCTTGGTTGGAGTGATTACCGCCGATAC
TGCGCTGAATTTGCCCGATTTTCGCTCGGGCGAGCGCACATTTCAATTATTGGCTCAAGTGGCGGGGCGAGCAGGGCGGC
GGGCCGGCGGCGGTCAAGTGATTATCCAAAGCTATAATCCTGAACATTATGCGATTCAGGCCGCCGCCCAGCACGATTAT
CGTCAATTTTACCGCGAAGAAATTGCCTTTCGCCGCACCGCCCACTATCCACCATTCAGCCGCTTGATTCGCTTTATGAC
CCAATCGCCATCGAACAAAACCGTTGAGCGGGTTGTGCGCCAATTCGATACCCAATTGCGCGAGGAGCTAGAACGGCGGC
GCATTCGCGATTGGGCGATTATTGGGCCATCGCCAGCCTTTTTGCAAAAAGTGCGCGATCTCTATCGTTGGCATATTTTG
GTGCGTTTGCCCGACCCCACTGATTTATTGCACGATTTAAAAATTCCCTCAGGCTGGACGATCGATGTTGATCCGGTTAG
TTTATTGTGA

Upstream 100 bases:

>100_bases
TATCCCTCATAATTCATCCTTCATAATTAAATTTTGGGCTAGCAAGGATGTGCTATGATGAAGCTGCGCTGTTTTGTGTG
AAGGAAATGCATTCGCCGTT

Downstream 100 bases:

>100_bases
TCCACAAAGCGCACGAAGAACACAAAGAATATATAAACGGTTATCCTTCGTGCTCTTCGTGTTCTTCGTGGATCAATCAC
ATGAGATCTGATCACTCACC

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MYAQVALNAYLPDNPEAILTYAVPPHLRPLRVGALVWVPLGPRRVQGVVLEFSTQRPQGFVVRDILEHADPEAIAAPAQL
ALAQWFSQSQAVRLWDAVQLTMPPGVKQEIERTWRPTEHGQKIELGTLDERERAILFHLRRVGEQSERQLRVDLRGSDAD
LRRSYTELHERGLIALGSLVSRPAAKPKREWSAQPTSATPAEALAELSKAPRLAAIFQAILEQAVDGEPVLLPELRDVGA
SLSHLRSLALRNLIQLEQIEVRRDPLLGRIPPLEFPPELTLEQERGWRQIVAAQDTASRTGKAKPILLHGVTGSGKTELY
LRAIGRALRNGGQALVLVPEIALTTQLVRRFAARFPQQLAVLHSELSVGERYDEWRRLRRGDAQVAIGSRSAIWSPMSKL
QLIIVDEEHEHSFKHEATPYYHARDLAVKLAELAGAVLILGSATPSLETAYAVQINKYEVLELHERVGRKTVAGVTKADP
IPMPPVQIVDMRSELKAGVRSMFSRPLQSAIQRTLDKREQTILFLNRRGIASFVMCRDCGTTIQCPRCAVALAVHATAGD
AAAPATLRCHYCGHVDWVPRNCPSCFSTRIRDFGVGTQRVVEEVKLLYPQARVMRWDRDVTGRKGSHEALLNQFTQHEAD
ILVGTQMVAKGFDLPLVTLVGVITADTALNLPDFRSGERTFQLLAQVAGRAGRRAGGGQVIIQSYNPEHYAIQAAAQHDY
RQFYREEIAFRRTAHYPPFSRLIRFMTQSPSNKTVERVVRQFDTQLREELERRRIRDWAIIGPSPAFLQKVRDLYRWHIL
VRLPDPTDLLHDLKIPSGWTIDVDPVSLL

Sequences:

>Translated_829_residues
MYAQVALNAYLPDNPEAILTYAVPPHLRPLRVGALVWVPLGPRRVQGVVLEFSTQRPQGFVVRDILEHADPEAIAAPAQL
ALAQWFSQSQAVRLWDAVQLTMPPGVKQEIERTWRPTEHGQKIELGTLDERERAILFHLRRVGEQSERQLRVDLRGSDAD
LRRSYTELHERGLIALGSLVSRPAAKPKREWSAQPTSATPAEALAELSKAPRLAAIFQAILEQAVDGEPVLLPELRDVGA
SLSHLRSLALRNLIQLEQIEVRRDPLLGRIPPLEFPPELTLEQERGWRQIVAAQDTASRTGKAKPILLHGVTGSGKTELY
LRAIGRALRNGGQALVLVPEIALTTQLVRRFAARFPQQLAVLHSELSVGERYDEWRRLRRGDAQVAIGSRSAIWSPMSKL
QLIIVDEEHEHSFKHEATPYYHARDLAVKLAELAGAVLILGSATPSLETAYAVQINKYEVLELHERVGRKTVAGVTKADP
IPMPPVQIVDMRSELKAGVRSMFSRPLQSAIQRTLDKREQTILFLNRRGIASFVMCRDCGTTIQCPRCAVALAVHATAGD
AAAPATLRCHYCGHVDWVPRNCPSCFSTRIRDFGVGTQRVVEEVKLLYPQARVMRWDRDVTGRKGSHEALLNQFTQHEAD
ILVGTQMVAKGFDLPLVTLVGVITADTALNLPDFRSGERTFQLLAQVAGRAGRRAGGGQVIIQSYNPEHYAIQAAAQHDY
RQFYREEIAFRRTAHYPPFSRLIRFMTQSPSNKTVERVVRQFDTQLREELERRRIRDWAIIGPSPAFLQKVRDLYRWHIL
VRLPDPTDLLHDLKIPSGWTIDVDPVSLL
>Mature_829_residues
MYAQVALNAYLPDNPEAILTYAVPPHLRPLRVGALVWVPLGPRRVQGVVLEFSTQRPQGFVVRDILEHADPEAIAAPAQL
ALAQWFSQSQAVRLWDAVQLTMPPGVKQEIERTWRPTEHGQKIELGTLDERERAILFHLRRVGEQSERQLRVDLRGSDAD
LRRSYTELHERGLIALGSLVSRPAAKPKREWSAQPTSATPAEALAELSKAPRLAAIFQAILEQAVDGEPVLLPELRDVGA
SLSHLRSLALRNLIQLEQIEVRRDPLLGRIPPLEFPPELTLEQERGWRQIVAAQDTASRTGKAKPILLHGVTGSGKTELY
LRAIGRALRNGGQALVLVPEIALTTQLVRRFAARFPQQLAVLHSELSVGERYDEWRRLRRGDAQVAIGSRSAIWSPMSKL
QLIIVDEEHEHSFKHEATPYYHARDLAVKLAELAGAVLILGSATPSLETAYAVQINKYEVLELHERVGRKTVAGVTKADP
IPMPPVQIVDMRSELKAGVRSMFSRPLQSAIQRTLDKREQTILFLNRRGIASFVMCRDCGTTIQCPRCAVALAVHATAGD
AAAPATLRCHYCGHVDWVPRNCPSCFSTRIRDFGVGTQRVVEEVKLLYPQARVMRWDRDVTGRKGSHEALLNQFTQHEAD
ILVGTQMVAKGFDLPLVTLVGVITADTALNLPDFRSGERTFQLLAQVAGRAGRRAGGGQVIIQSYNPEHYAIQAAAQHDY
RQFYREEIAFRRTAHYPPFSRLIRFMTQSPSNKTVERVVRQFDTQLREELERRRIRDWAIIGPSPAFLQKVRDLYRWHIL
VRLPDPTDLLHDLKIPSGWTIDVDPVSLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=562, Percent_Identity=38.4341637010676, Blast_Score=348, Evalue=9e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 93079; Mature: 93079

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAQVALNAYLPDNPEAILTYAVPPHLRPLRVGALVWVPLGPRRVQGVVLEFSTQRPQGF
CCCEEEEEECCCCCCCEEEEEECCCCCCCEEECEEEEECCCCHHHCEEEEEEECCCCCCH
VVRDILEHADPEAIAAPAQLALAQWFSQSQAVRLWDAVQLTMPPGVKQEIERTWRPTEHG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHEEEEECCCCHHHHHHHHCCCCCCC
QKIELGTLDERERAILFHLRRVGEQSERQLRVDLRGSDADLRRSYTELHERGLIALGSLV
CEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
SRPAAKPKREWSAQPTSATPAEALAELSKAPRLAAIFQAILEQAVDGEPVLLPELRDVGA
HCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCHHHHHH
SLSHLRSLALRNLIQLEQIEVRRDPLLGRIPPLEFPPELTLEQERGWRQIVAAQDTASRT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHCCCHHHCC
GKAKPILLHGVTGSGKTELYLRAIGRALRNGGQALVLVPEIALTTQLVRRFAARFPQQLA
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCHHHHH
VLHSELSVGERYDEWRRLRRGDAQVAIGSRSAIWSPMSKLQLIIVDEEHEHSFKHEATPY
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCC
YHARDLAVKLAELAGAVLILGSATPSLETAYAVQINKYEVLELHERVGRKTVAGVTKADP
CHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHCCHHHCCCCCCCC
IPMPPVQIVDMRSELKAGVRSMFSRPLQSAIQRTLDKREQTILFLNRRGIASFVMCRDCG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCC
TTIQCPRCAVALAVHATAGDAAAPATLRCHYCGHVDWVPRNCPSCFSTRIRDFGVGTQRV
CCCCCCHHEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHH
VEEVKLLYPQARVMRWDRDVTGRKGSHEALLNQFTQHEADILVGTQMVAKGFDLPLVTLV
HHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GVITADTALNLPDFRSGERTFQLLAQVAGRAGRRAGGGQVIIQSYNPEHYAIQAAAQHDY
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEHHHHHHH
RQFYREEIAFRRTAHYPPFSRLIRFMTQSPSNKTVERVVRQFDTQLREELERRRIRDWAI
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
IGPSPAFLQKVRDLYRWHILVRLPDPTDLLHDLKIPSGWTIDVDPVSLL
ECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MYAQVALNAYLPDNPEAILTYAVPPHLRPLRVGALVWVPLGPRRVQGVVLEFSTQRPQGF
CCCEEEEEECCCCCCCEEEEEECCCCCCCEEECEEEEECCCCHHHCEEEEEEECCCCCCH
VVRDILEHADPEAIAAPAQLALAQWFSQSQAVRLWDAVQLTMPPGVKQEIERTWRPTEHG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHEEEEECCCCHHHHHHHHCCCCCCC
QKIELGTLDERERAILFHLRRVGEQSERQLRVDLRGSDADLRRSYTELHERGLIALGSLV
CEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
SRPAAKPKREWSAQPTSATPAEALAELSKAPRLAAIFQAILEQAVDGEPVLLPELRDVGA
HCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCHHHHHH
SLSHLRSLALRNLIQLEQIEVRRDPLLGRIPPLEFPPELTLEQERGWRQIVAAQDTASRT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHCCCHHHCC
GKAKPILLHGVTGSGKTELYLRAIGRALRNGGQALVLVPEIALTTQLVRRFAARFPQQLA
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCHHHHH
VLHSELSVGERYDEWRRLRRGDAQVAIGSRSAIWSPMSKLQLIIVDEEHEHSFKHEATPY
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCC
YHARDLAVKLAELAGAVLILGSATPSLETAYAVQINKYEVLELHERVGRKTVAGVTKADP
CHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHCCHHHCCCCCCCC
IPMPPVQIVDMRSELKAGVRSMFSRPLQSAIQRTLDKREQTILFLNRRGIASFVMCRDCG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCC
TTIQCPRCAVALAVHATAGDAAAPATLRCHYCGHVDWVPRNCPSCFSTRIRDFGVGTQRV
CCCCCCHHEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHH
VEEVKLLYPQARVMRWDRDVTGRKGSHEALLNQFTQHEADILVGTQMVAKGFDLPLVTLV
HHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GVITADTALNLPDFRSGERTFQLLAQVAGRAGRRAGGGQVIIQSYNPEHYAIQAAAQHDY
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEHHHHHHH
RQFYREEIAFRRTAHYPPFSRLIRFMTQSPSNKTVERVVRQFDTQLREELERRRIRDWAI
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
IGPSPAFLQKVRDLYRWHILVRLPDPTDLLHDLKIPSGWTIDVDPVSLL
ECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]