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The map label for this gene is pfkA [H]

Identifier: 159900615

GI number: 159900615

Start: 5234608

End: 5235603

Strand: Reverse

Name: pfkA [H]

Synonym: Haur_4102

Alternate gene names: 159900615

Gene position: 5235603-5234608 (Counterclockwise)

Preceding gene: 159900617

Following gene: 159900614

Centisome position: 82.49

GC content: 51.71

Gene sequence:

>996_bases
ATGCATCGCATTGCCGTTTTAACCAGTGGTGGCGACTCCCCAGGGATGAATCCCGCAATTCGTGCAGTCACGCGGACTGC
ACTCCATGCAGGTTGTGAAGTGTTTGGCGTGTATCGAGGCTATGCTGGCCTCATTCAAAATGATATGCGTCGGCTTGAAT
ATAAAGATGTTGGCGGCATTATCCAACGTGGTGGCACAATGCTGCTCACCGCCCGCTCGCCCGAATTTATGACTCCCGCT
GGCCGTGAAAAAGCCATGGAGAATTTGCGCTGGCATGGCATTGAAGGCTTGGTGGTAATTGGCGGCGATGGTTCGTTGCG
TGGGGCCGCTGCCCTAACCGCCGAACATGGCCTGCCCACAATCTGCTTACCAGGCACAATCGATAACGATATGTATGGCA
CTGATATGAGCATTGGTGCTGATACGGCGCTCAACACGGTGCTGGAAGCAATCGATAAAATTAAAGATACCGCTTCATCA
CACCAACGCGCTTTTATCATCGAAACCATGGGTCGTCACTCAGGCTATTTGGCCTTGATGGGCGGCTTGGCTGGCGGCGC
TGAGGCAATTCTGATTCCCGAAATTCCCGATACCACAATTGAAGATGTGGTTGAAGTGATCAAGCGTTCGTTTCGCAAGG
GTAAAACCAACTGCGTGATTGTGGCAGCCGAAGGGGCGCATTTGAACGCCCAAACCATCCAAGACCAAATTAACCAAATC
GATGCTGAGGAAAGCGGTTTTAGCTTTAGCTTCCGCACGCGGGCCACAATTTTGGGTCATATTCAGCGTGGCGGTTCGCC
CTCGGCCTTTGATCGTACTTTGGCAACCCGGCTTGGTTCGGGCGCGGTCGAGTTTTTGCTATCAGGCAAGAATGGTGGTT
TGGTTGGCATGCAATCACGCAAATTAACCGTCACACCCTATGATGAGGTGCTGGCGAATCCCAAAGTGCTTGATGCCAAG
CTCTACGAACTTTCAAAAATTCTTGATCGCTACTAA

Upstream 100 bases:

>100_bases
CTGGTGGCTTTTACGAAAAGTGTTTTGAACTCAATGGCAAGGGAGCCATCGACGGCAATGTCGCCGCTTCAAGCATTAAT
AACTTCAATGAGGTTTGATC

Downstream 100 bases:

>100_bases
AGAACAAAGAGCATAGAACATAGAACAGAGCGATCATGAGGACTTAATTGATCCGACGAGCGAAAAGAGCATAGATGAAT
GTTGTTCTATGCTCTTTGTT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MHRIAVLTSGGDSPGMNPAIRAVTRTALHAGCEVFGVYRGYAGLIQNDMRRLEYKDVGGIIQRGGTMLLTARSPEFMTPA
GREKAMENLRWHGIEGLVVIGGDGSLRGAAALTAEHGLPTICLPGTIDNDMYGTDMSIGADTALNTVLEAIDKIKDTASS
HQRAFIIETMGRHSGYLALMGGLAGGAEAILIPEIPDTTIEDVVEVIKRSFRKGKTNCVIVAAEGAHLNAQTIQDQINQI
DAEESGFSFSFRTRATILGHIQRGGSPSAFDRTLATRLGSGAVEFLLSGKNGGLVGMQSRKLTVTPYDEVLANPKVLDAK
LYELSKILDRY

Sequences:

>Translated_331_residues
MHRIAVLTSGGDSPGMNPAIRAVTRTALHAGCEVFGVYRGYAGLIQNDMRRLEYKDVGGIIQRGGTMLLTARSPEFMTPA
GREKAMENLRWHGIEGLVVIGGDGSLRGAAALTAEHGLPTICLPGTIDNDMYGTDMSIGADTALNTVLEAIDKIKDTASS
HQRAFIIETMGRHSGYLALMGGLAGGAEAILIPEIPDTTIEDVVEVIKRSFRKGKTNCVIVAAEGAHLNAQTIQDQINQI
DAEESGFSFSFRTRATILGHIQRGGSPSAFDRTLATRLGSGAVEFLLSGKNGGLVGMQSRKLTVTPYDEVLANPKVLDAK
LYELSKILDRY
>Mature_331_residues
MHRIAVLTSGGDSPGMNPAIRAVTRTALHAGCEVFGVYRGYAGLIQNDMRRLEYKDVGGIIQRGGTMLLTARSPEFMTPA
GREKAMENLRWHGIEGLVVIGGDGSLRGAAALTAEHGLPTICLPGTIDNDMYGTDMSIGADTALNTVLEAIDKIKDTASS
HQRAFIIETMGRHSGYLALMGGLAGGAEAILIPEIPDTTIEDVVEVIKRSFRKGKTNCVIVAAEGAHLNAQTIQDQINQI
DAEESGFSFSFRTRATILGHIQRGGSPSAFDRTLATRLGSGAVEFLLSGKNGGLVGMQSRKLTVTPYDEVLANPKVLDAK
LYELSKILDRY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=316, Percent_Identity=42.7215189873418, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI11321601, Length=313, Percent_Identity=42.4920127795527, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI266453768, Length=355, Percent_Identity=39.1549295774648, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI266453748, Length=355, Percent_Identity=39.1549295774648, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4505749, Length=355, Percent_Identity=39.1549295774648, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI266453619, Length=355, Percent_Identity=39.1549295774648, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI1790350, Length=315, Percent_Identity=48.2539682539683, Blast_Score=270, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI25147584, Length=316, Percent_Identity=41.4556962025316, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17558788, Length=313, Percent_Identity=38.6581469648562, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI72003601, Length=332, Percent_Identity=33.433734939759, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321679, Length=315, Percent_Identity=45.0793650793651, Blast_Score=245, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6323861, Length=342, Percent_Identity=37.4269005847953, Blast_Score=198, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24652337, Length=316, Percent_Identity=42.4050632911392, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI17647809, Length=316, Percent_Identity=42.4050632911392, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI28573326, Length=316, Percent_Identity=41.1392405063291, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35402; Mature: 35402

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRIAVLTSGGDSPGMNPAIRAVTRTALHAGCEVFGVYRGYAGLIQNDMRRLEYKDVGGI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCHHHEEHHHHHHHHHHHHHHHHHHHHHHHH
IQRGGTMLLTARSPEFMTPAGREKAMENLRWHGIEGLVVIGGDGSLRGAAALTAEHGLPT
HHCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCE
ICLPGTIDNDMYGTDMSIGADTALNTVLEAIDKIKDTASSHQRAFIIETMGRHSGYLALM
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEE
GGLAGGAEAILIPEIPDTTIEDVVEVIKRSFRKGKTNCVIVAAEGAHLNAQTIQDQINQI
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHC
DAEESGFSFSFRTRATILGHIQRGGSPSAFDRTLATRLGSGAVEFLLSGKNGGLVGMQSR
CCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCC
KLTVTPYDEVLANPKVLDAKLYELSKILDRY
EEEECCHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHRIAVLTSGGDSPGMNPAIRAVTRTALHAGCEVFGVYRGYAGLIQNDMRRLEYKDVGGI
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCHHHEEHHHHHHHHHHHHHHHHHHHHHHHH
IQRGGTMLLTARSPEFMTPAGREKAMENLRWHGIEGLVVIGGDGSLRGAAALTAEHGLPT
HHCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCE
ICLPGTIDNDMYGTDMSIGADTALNTVLEAIDKIKDTASSHQRAFIIETMGRHSGYLALM
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEE
GGLAGGAEAILIPEIPDTTIEDVVEVIKRSFRKGKTNCVIVAAEGAHLNAQTIQDQINQI
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHC
DAEESGFSFSFRTRATILGHIQRGGSPSAFDRTLATRLGSGAVEFLLSGKNGGLVGMQSR
CCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCC
KLTVTPYDEVLANPKVLDAKLYELSKILDRY
EEEECCHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA