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The map label for this gene is rsrIM [H]

Identifier: 159900577

GI number: 159900577

Start: 5189510

End: 5190355

Strand: Direct

Name: rsrIM [H]

Synonym: Haur_4064

Alternate gene names: 159900577

Gene position: 5189510-5190355 (Clockwise)

Preceding gene: 159900576

Following gene: 159900579

Centisome position: 81.77

GC content: 47.4

Gene sequence:

>846_bases
ATGAATAAACTTTATTTTGGCGATAATTTGGCTGTTTTGGCAACCCTGCCAGCGGCCAGCTATGATTTAATTTATATTGA
CCCGCCCTTCAACACTGGCAAAATCCAAAGTCGCACCCAACTGCGCACGGTTCGCTCCGAGCAAGGTGATCGGGTTGGCT
TTGGTGGTCATCGTTATAGCAGCATCAAAATTGGCGAGCGAGCCTATGGCGATAGCTTTGACGATTTTCTGGCATTTATT
GAGCCGCGTTTGCTTGAAGCTTACCGTTTGCTGAAACCCCAAGGCAGCTTCTTTTTTCACATCGATTATCGTGAAGTCCA
TTATTGCAAGGTATTGATCGACCAAATTTTTGGCCGTGATTCGTTTATCAACGAAATTATTTGGGCCTACGATTATGGCG
CTCGGTCGCGCAAAAAATGGTCAACCAAGCACGATACCATCTTGTGGTATGCCAAAGATCCCGAAAACTATACCTTTAAT
TATGATCAGATTGATCGCATTCCCTATATGGCTCCAGGTTTGGTTGGCCCCGAAAAGGCTGCCCGTGGCAAAACGCCGAC
CGATGTTTGGTGGAACACGATTGTTAGCCCCAATGGCAAGGAAAAAACTGGCTATCCGACCCAAAAACCACTAGCAATTC
TCAACCGGATTGTGCGCGTCCACTCCAACCCCAACGATCAACTGCTCGATTTCTTTGCTGGCAGCGGCTCGTTTGGTGAG
GCGGCTGCGCGGAATGGCCGTAATTTCACCTTGATCGACCAAAATCCCCAAGCAATTGAGGTGATGCGCCAACGTTTGGC
CTTCGCTGAGCCAGAATTTTACGAAATACCCAAGGATATAATTTAA

Upstream 100 bases:

>100_bases
AGTGGCTGTGATTCGGCAATTTTACGGGCTTGGTTGGAAGGCAAGCGTTCATTGAGTGAAGCCGAACGCTTAGCGATTGA
GGAAGCATTAACATCACACC

Downstream 100 bases:

>100_bases
CCACGAAGGACACGAAGTTTCACTAAGGTTTGGGTTATTGGTTCTCTGATGTCTTGTACAAAACTCAGGTTTCCAACGAG
CCTAACATTCGTGCTCTTCG

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.RsrI; Adenine-specific methyltransferase RsrI [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNKLYFGDNLAVLATLPAASYDLIYIDPPFNTGKIQSRTQLRTVRSEQGDRVGFGGHRYSSIKIGERAYGDSFDDFLAFI
EPRLLEAYRLLKPQGSFFFHIDYREVHYCKVLIDQIFGRDSFINEIIWAYDYGARSRKKWSTKHDTILWYAKDPENYTFN
YDQIDRIPYMAPGLVGPEKAARGKTPTDVWWNTIVSPNGKEKTGYPTQKPLAILNRIVRVHSNPNDQLLDFFAGSGSFGE
AAARNGRNFTLIDQNPQAIEVMRQRLAFAEPEFYEIPKDII

Sequences:

>Translated_281_residues
MNKLYFGDNLAVLATLPAASYDLIYIDPPFNTGKIQSRTQLRTVRSEQGDRVGFGGHRYSSIKIGERAYGDSFDDFLAFI
EPRLLEAYRLLKPQGSFFFHIDYREVHYCKVLIDQIFGRDSFINEIIWAYDYGARSRKKWSTKHDTILWYAKDPENYTFN
YDQIDRIPYMAPGLVGPEKAARGKTPTDVWWNTIVSPNGKEKTGYPTQKPLAILNRIVRVHSNPNDQLLDFFAGSGSFGE
AAARNGRNFTLIDQNPQAIEVMRQRLAFAEPEFYEIPKDII
>Mature_281_residues
MNKLYFGDNLAVLATLPAASYDLIYIDPPFNTGKIQSRTQLRTVRSEQGDRVGFGGHRYSSIKIGERAYGDSFDDFLAFI
EPRLLEAYRLLKPQGSFFFHIDYREVHYCKVLIDQIFGRDSFINEIIWAYDYGARSRKKWSTKHDTILWYAKDPENYTFN
YDQIDRIPYMAPGLVGPEKAARGKTPTDVWWNTIVSPNGKEKTGYPTQKPLAILNRIVRVHSNPNDQLLDFFAGSGSFGE
AAARNGRNFTLIDQNPQAIEVMRQRLAFAEPEFYEIPKDII

Specific function: This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the RsrI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=279, Percent_Identity=31.1827956989247, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32329; Mature: 32329

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLYFGDNLAVLATLPAASYDLIYIDPPFNTGKIQSRTQLRTVRSEQGDRVGFGGHRYS
CCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEECCCCCEEC
SIKIGERAYGDSFDDFLAFIEPRLLEAYRLLKPQGSFFFHIDYREVHYCKVLIDQIFGRD
EEEECCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCH
SFINEIIWAYDYGARSRKKWSTKHDTILWYAKDPENYTFNYDQIDRIPYMAPGLVGPEKA
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEECHHHHCCCCCCCCCCCCCHHH
ARGKTPTDVWWNTIVSPNGKEKTGYPTQKPLAILNRIVRVHSNPNDQLLDFFAGSGSFGE
HCCCCCHHHHHHCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCH
AAARNGRNFTLIDQNPQAIEVMRQRLAFAEPEFYEIPKDII
HHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCC
>Mature Secondary Structure
MNKLYFGDNLAVLATLPAASYDLIYIDPPFNTGKIQSRTQLRTVRSEQGDRVGFGGHRYS
CCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEECCCCCEEC
SIKIGERAYGDSFDDFLAFIEPRLLEAYRLLKPQGSFFFHIDYREVHYCKVLIDQIFGRD
EEEECCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCH
SFINEIIWAYDYGARSRKKWSTKHDTILWYAKDPENYTFNYDQIDRIPYMAPGLVGPEKA
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEECHHHHCCCCCCCCCCCCCHHH
ARGKTPTDVWWNTIVSPNGKEKTGYPTQKPLAILNRIVRVHSNPNDQLLDFFAGSGSFGE
HCCCCCHHHHHHCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCH
AAARNGRNFTLIDQNPQAIEVMRQRLAFAEPEFYEIPKDII
HHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2690017; 2602165; 11024175 [H]