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The map label for this gene is asnB [H]

Identifier: 159900518

GI number: 159900518

Start: 5111356

End: 5113224

Strand: Reverse

Name: asnB [H]

Synonym: Haur_4005

Alternate gene names: 159900518

Gene position: 5113224-5111356 (Counterclockwise)

Preceding gene: 159900519

Following gene: 159900517

Centisome position: 80.57

GC content: 53.29

Gene sequence:

>1869_bases
ATGTGTGGCATTACGGGCCATCTCGAATGGCATGGCACGGCGCAAACCGCCATTCTTGAGCAGCAAACCGAGGCGATTCG
CCATCGTGGGCCGGATAGTGCGGGCTTTTTTTGCGTTGCTCAGGTGGCGTTGGGCATGCGGCGCTTGGCAATTATCGACC
TAACCAGTGGCGATCAGCCGATGTTTAGTCGTGATGGCGATTTAGCCTTGGTGTTCAACGGCGAAATTTATAATTTTCAG
GCTTTGCGCCAGCAATTGCAGCAACTCGGCCAACATTTCGCCACCAACAGCGATACCGAAGTGATTGTGCGGGGGATTGA
GCAATGGGGTATTTTGGGCTGTGTGCAACGCTTGAATGGCATGTTTGCCTTGGCGATTTGGCAGCAACAAGCCCAACGCC
TGACTTTGGTGCGCGATCGACTTGGGATCAAGCCTCTCTATTGGTATTCCGATGCTCAGCGCTTGGTGTTTGGCTCGGAA
ATCAAGGCAATTTTGGCGCATCCAGCAGTGCCACGCCAAATCAATCTGCAAGGTTTGGCTAATTATCTAAGTTTTGGCCA
TAGCCTCGCACCCCAAACCATGCTCCAAGCTATTTATAAAGTCGAGCCAGCCCACATTCTGACATGGGATGTTGCTAGCC
GTGCCATGCAAACCCAACGCTATTGGCAATTGCAGCCCAATCCAATCGCGATTGCGCCCGCCGAAGCTGCCGCCGAATGC
TATAGCCGTTTGCGCGAAGCTGTGCGGTTGCAACTGATTAGCGATGTGCCGCTGGGTGCGTTTCTTAGTGGCGGGCTTGA
TTCAAGTATTATCGTGGGCTTGATGCAGCGCTTGGGAGCAGCACCAATCAACACATTTAGCGTGGGCTTTGAGCATGCAG
GCTTCAACGAATTGCCCGATGCAGCACTCGTGGCCCAGCATTTTGGCACTAAACATCATGAACTGCGCCTGAATGCCAAC
GATTTGGTTGGCGCTTTGCAAACCTTGGTTTATCACTACGACGAACCATTTGGCGATGCCGCAGGCTTGCCGGTATATCT
CGTTTCACGCTTTGCCCGCGAACATGTCAAAGTGGTGCTGACGGGCGAGGGCAGCGATGAGCAATGGGCTGGTTATCGGC
GCTATCAAGCCGAATTGATCGCTCGGATGGCGCAGTATTTGCCTGGGCGTAGCGCAATTGCCGCGCTGATTCGCCAGTTG
CCCCGTAATCGCCGCCTCAAACAGGCAATTCGCACCTTAGATCAGCGTGATCCCGGGCGGCGTTACGCTGCATGGCTGAC
GATTGCCGATGTGCAACAGCGCCAACGCTTGCTGCAACCACATATCAGCGCAGTGCTAGGGGATTATGCGCCTGAGCAAA
TCTATGATCAGGTGTATCCGCGCAGCGGCGCAGCCATGACCAACATGGGCTTGGCCGATTTACAAACATGGTTGCCAGAT
ACCTATTTGGAAAAAGTTGATAAGGCCTCGATGGCTGCTAGTATCGAGGCGCGGGTGCCATTTCTTGACCATACCTTGGT
GGAATGGACGATGAATTTGCCGCCAACACTTAAACTGCGTGGCACAAAAACCAAGTGGTTGCTCCGCCAAGCTTTTGGCG
AAATGCTGCCGCAACGCACTTTGCGCAAGCCTAAACATGGCTTTGCAGTGCCAACCGACCCATGGTTTCGCGGAGCATTG
AGCAACTGGACTGCCGAAATTTTGTTTGATCAACGCACGCTCAGCCGTGGTTTGTTCAATCCGCACGAAGTCCAACGGAT
CTACCAAGCCCATCGCGATGGCAAAGAAACCGCAGATACCGTATTATGGTTACTACTGAACATCGAACTTTGGCAACGGA
TCTATCTTGATCGCGAGGAACGGCTATGA

Upstream 100 bases:

>100_bases
GAACGATCAAACTTTTACCCTTCTCATCAAGCAGAGAAGGGTTTCGTTAATTTTTGCTCCAGCATTGTTCAATCACTATT
ACTCTCATTTGGAGCGTTGT

Downstream 100 bases:

>100_bases
ATCAACCTTTAGTGCTGCATATTGCAACTGCCGATATGGGCTTGCGCTTTTTACTGCTCGAACAGATGCAGGCAATTCAT
CATGCAGGCTATCAGGTACG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MCGITGHLEWHGTAQTAILEQQTEAIRHRGPDSAGFFCVAQVALGMRRLAIIDLTSGDQPMFSRDGDLALVFNGEIYNFQ
ALRQQLQQLGQHFATNSDTEVIVRGIEQWGILGCVQRLNGMFALAIWQQQAQRLTLVRDRLGIKPLYWYSDAQRLVFGSE
IKAILAHPAVPRQINLQGLANYLSFGHSLAPQTMLQAIYKVEPAHILTWDVASRAMQTQRYWQLQPNPIAIAPAEAAAEC
YSRLREAVRLQLISDVPLGAFLSGGLDSSIIVGLMQRLGAAPINTFSVGFEHAGFNELPDAALVAQHFGTKHHELRLNAN
DLVGALQTLVYHYDEPFGDAAGLPVYLVSRFAREHVKVVLTGEGSDEQWAGYRRYQAELIARMAQYLPGRSAIAALIRQL
PRNRRLKQAIRTLDQRDPGRRYAAWLTIADVQQRQRLLQPHISAVLGDYAPEQIYDQVYPRSGAAMTNMGLADLQTWLPD
TYLEKVDKASMAASIEARVPFLDHTLVEWTMNLPPTLKLRGTKTKWLLRQAFGEMLPQRTLRKPKHGFAVPTDPWFRGAL
SNWTAEILFDQRTLSRGLFNPHEVQRIYQAHRDGKETADTVLWLLLNIELWQRIYLDREERL

Sequences:

>Translated_622_residues
MCGITGHLEWHGTAQTAILEQQTEAIRHRGPDSAGFFCVAQVALGMRRLAIIDLTSGDQPMFSRDGDLALVFNGEIYNFQ
ALRQQLQQLGQHFATNSDTEVIVRGIEQWGILGCVQRLNGMFALAIWQQQAQRLTLVRDRLGIKPLYWYSDAQRLVFGSE
IKAILAHPAVPRQINLQGLANYLSFGHSLAPQTMLQAIYKVEPAHILTWDVASRAMQTQRYWQLQPNPIAIAPAEAAAEC
YSRLREAVRLQLISDVPLGAFLSGGLDSSIIVGLMQRLGAAPINTFSVGFEHAGFNELPDAALVAQHFGTKHHELRLNAN
DLVGALQTLVYHYDEPFGDAAGLPVYLVSRFAREHVKVVLTGEGSDEQWAGYRRYQAELIARMAQYLPGRSAIAALIRQL
PRNRRLKQAIRTLDQRDPGRRYAAWLTIADVQQRQRLLQPHISAVLGDYAPEQIYDQVYPRSGAAMTNMGLADLQTWLPD
TYLEKVDKASMAASIEARVPFLDHTLVEWTMNLPPTLKLRGTKTKWLLRQAFGEMLPQRTLRKPKHGFAVPTDPWFRGAL
SNWTAEILFDQRTLSRGLFNPHEVQRIYQAHRDGKETADTVLWLLLNIELWQRIYLDREERL
>Mature_622_residues
MCGITGHLEWHGTAQTAILEQQTEAIRHRGPDSAGFFCVAQVALGMRRLAIIDLTSGDQPMFSRDGDLALVFNGEIYNFQ
ALRQQLQQLGQHFATNSDTEVIVRGIEQWGILGCVQRLNGMFALAIWQQQAQRLTLVRDRLGIKPLYWYSDAQRLVFGSE
IKAILAHPAVPRQINLQGLANYLSFGHSLAPQTMLQAIYKVEPAHILTWDVASRAMQTQRYWQLQPNPIAIAPAEAAAEC
YSRLREAVRLQLISDVPLGAFLSGGLDSSIIVGLMQRLGAAPINTFSVGFEHAGFNELPDAALVAQHFGTKHHELRLNAN
DLVGALQTLVYHYDEPFGDAAGLPVYLVSRFAREHVKVVLTGEGSDEQWAGYRRYQAELIARMAQYLPGRSAIAALIRQL
PRNRRLKQAIRTLDQRDPGRRYAAWLTIADVQQRQRLLQPHISAVLGDYAPEQIYDQVYPRSGAAMTNMGLADLQTWLPD
TYLEKVDKASMAASIEARVPFLDHTLVEWTMNLPPTLKLRGTKTKWLLRQAFGEMLPQRTLRKPKHGFAVPTDPWFRGAL
SNWTAEILFDQRTLSRGLFNPHEVQRIYQAHRDGKETADTVLWLLLNIELWQRIYLDREERL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=399, Percent_Identity=29.3233082706767, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI168229252, Length=399, Percent_Identity=29.3233082706767, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI168229250, Length=399, Percent_Identity=29.3233082706767, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI296010848, Length=371, Percent_Identity=29.6495956873315, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI296010852, Length=308, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI296010850, Length=308, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786889, Length=395, Percent_Identity=31.1392405063291, Blast_Score=156, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI25147557, Length=383, Percent_Identity=30.2872062663185, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71993933, Length=383, Percent_Identity=30.2872062663185, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=331, Percent_Identity=29.9093655589124, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321563, Length=415, Percent_Identity=29.3975903614458, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325403, Length=416, Percent_Identity=29.3269230769231, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45553209, Length=397, Percent_Identity=28.463476070529, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70319; Mature: 70319

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGHLEWHGTAQTAILEQQTEAIRHRGPDSAGFFCVAQVALGMRRLAIIDLTSGDQP
CCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCC
MFSRDGDLALVFNGEIYNFQALRQQLQQLGQHFATNSDTEVIVRGIEQWGILGCVQRLNG
CCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
MFALAIWQQQAQRLTLVRDRLGIKPLYWYSDAQRLVFGSEIKAILAHPAVPRQINLQGLA
CEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEECHHHHH
NYLSFGHSLAPQTMLQAIYKVEPAHILTWDVASRAMQTQRYWQLQPNPIAIAPAEAAAEC
HHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHEEECCCCEEEECHHHHHHH
YSRLREAVRLQLISDVPLGAFLSGGLDSSIIVGLMQRLGAAPINTFSVGFEHAGFNELPD
HHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCH
AALVAQHFGTKHHELRLNANDLVGALQTLVYHYDEPFGDAAGLPVYLVSRFAREHVKVVL
HHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEE
TGEGSDEQWAGYRRYQAELIARMAQYLPGRSAIAALIRQLPRNRRLKQAIRTLDQRDPGR
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
RYAAWLTIADVQQRQRLLQPHISAVLGDYAPEQIYDQVYPRSGAAMTNMGLADLQTWLPD
HHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCHHHHHHHCCH
TYLEKVDKASMAASIEARVPFLDHTLVEWTMNLPPTLKLRGTKTKWLLRQAFGEMLPQRT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCHHH
LRKPKHGFAVPTDPWFRGALSNWTAEILFDQRTLSRGLFNPHEVQRIYQAHRDGKETADT
HCCCCCCCCCCCCHHHHHHHHCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
VLWLLLNIELWQRIYLDREERL
HHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MCGITGHLEWHGTAQTAILEQQTEAIRHRGPDSAGFFCVAQVALGMRRLAIIDLTSGDQP
CCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCC
MFSRDGDLALVFNGEIYNFQALRQQLQQLGQHFATNSDTEVIVRGIEQWGILGCVQRLNG
CCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
MFALAIWQQQAQRLTLVRDRLGIKPLYWYSDAQRLVFGSEIKAILAHPAVPRQINLQGLA
CEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEECHHHHH
NYLSFGHSLAPQTMLQAIYKVEPAHILTWDVASRAMQTQRYWQLQPNPIAIAPAEAAAEC
HHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHEEECCCCEEEECHHHHHHH
YSRLREAVRLQLISDVPLGAFLSGGLDSSIIVGLMQRLGAAPINTFSVGFEHAGFNELPD
HHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCH
AALVAQHFGTKHHELRLNANDLVGALQTLVYHYDEPFGDAAGLPVYLVSRFAREHVKVVL
HHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEE
TGEGSDEQWAGYRRYQAELIARMAQYLPGRSAIAALIRQLPRNRRLKQAIRTLDQRDPGR
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
RYAAWLTIADVQQRQRLLQPHISAVLGDYAPEQIYDQVYPRSGAAMTNMGLADLQTWLPD
HHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCHHHHHHHCCH
TYLEKVDKASMAASIEARVPFLDHTLVEWTMNLPPTLKLRGTKTKWLLRQAFGEMLPQRT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCHHH
LRKPKHGFAVPTDPWFRGALSNWTAEILFDQRTLSRGLFNPHEVQRIYQAHRDGKETADT
HCCCCCCCCCCCCHHHHHHHHCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
VLWLLLNIELWQRIYLDREERL
HHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]