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The map label for this gene is rffG [C]

Identifier: 159900414

GI number: 159900414

Start: 4891246

End: 4892235

Strand: Reverse

Name: rffG [C]

Synonym: Haur_3899

Alternate gene names: 159900414

Gene position: 4892235-4891246 (Counterclockwise)

Preceding gene: 159900415

Following gene: 159900413

Centisome position: 77.08

GC content: 49.19

Gene sequence:

>990_bases
GTGCTAGAGCAGGCATTTCGTGGCAAGCGTTGTTTGATTACTGGCGGTTTGGGCTTTATTGGCTCGAATCTCGCATTTCG
TTTGGTCGAATTAGGCGCTGAAGTTGTGATCGTTGATTCATTGGTTGCTGAGTATGGCGGCAATATGGCCAATGTCCATG
GCTTGGCTGAACAAGTGCGGATTAACATTGCCGATGTCCGCGATCAGCATTCGTTGCAATATCTGGTACAAGGCCAGGAT
TATCTGTTTAATCTTGCTGGTCAGGTTAGCCATATTGATTCAATGGAGAATCCCTTCAACGACTTGGATATTAATTGTCG
GGCGCAGCTTTCAATTTTAGAGGCTTGTCGCCACAATAATCCAGCGATTAAAATTGTTTTTGCGAGCACCCGCCAAATCT
ATGGCCGCCCCGATTACGTGCCTGTTGATGAGCAGCATTTGTTGCACCCAACCGATGTCAATGGCATTAACAAGATGGCG
GGCGAGTGGTATCACATTCTGTATAATAATGTCTATGGGATTCGGGCTTGTGCTTTGCGCATGACCAATACCTATGGGCC
GCGCATGTTGGTCCGCCATGCTCGCCAAACTGCCTTGGGCTGGTTTGTGCGCCAAGCACTTGATAACGAAGTGATTAGCA
TTTATGGCGATGGCGAGCAACAGCGTGATTATACCTATGTTGATGATGCAGTTGAAGCTTTTCTGTTAGCAGCCATGAAT
CCGGCTGCCGATGGTCAGGTGTTTAATCTCGGCGGCCCTGAGCCAATTAGCCATTTGCAATTGATTTCGACCTTGACGGA
AGTTGCTGGAACTGGCTCGTATCGACTCGTGCCGTTTCCCCCTGAAAAAGCCCGCATCGACATTGGCAGCGTCTATAGCG
ACTACAGCCGGATTCAAGCGGTGTTGGGTTGGCAGCCAACCACGAGTTTGCGCCAAGGTCTCGAGTATACCGTAAACTTC
TACCGCGAATTCCGCGAATCCTATTGGTAG

Upstream 100 bases:

>100_bases
GGTGGAAGTTGGTCATCCTTGGCAAACATCTTAAAGAACAAGCTGCTCGCCAACGTGCTCTTGGGCGCGACGCGGGCTAA
CGGCTATTTATGGAGGTTGT

Downstream 100 bases:

>100_bases
TTGGTTTCCCTCACCCAACCTCGCCCTCAGTGCGGCGTGAGATAAAAGGATCGGTCTTAATCTCTGGCTTGGGAACGACG
ATCCCATAGTTCATCGGTGA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLEQAFRGKRCLITGGLGFIGSNLAFRLVELGAEVVIVDSLVAEYGGNMANVHGLAEQVRINIADVRDQHSLQYLVQGQD
YLFNLAGQVSHIDSMENPFNDLDINCRAQLSILEACRHNNPAIKIVFASTRQIYGRPDYVPVDEQHLLHPTDVNGINKMA
GEWYHILYNNVYGIRACALRMTNTYGPRMLVRHARQTALGWFVRQALDNEVISIYGDGEQQRDYTYVDDAVEAFLLAAMN
PAADGQVFNLGGPEPISHLQLISTLTEVAGTGSYRLVPFPPEKARIDIGSVYSDYSRIQAVLGWQPTTSLRQGLEYTVNF
YREFRESYW

Sequences:

>Translated_329_residues
MLEQAFRGKRCLITGGLGFIGSNLAFRLVELGAEVVIVDSLVAEYGGNMANVHGLAEQVRINIADVRDQHSLQYLVQGQD
YLFNLAGQVSHIDSMENPFNDLDINCRAQLSILEACRHNNPAIKIVFASTRQIYGRPDYVPVDEQHLLHPTDVNGINKMA
GEWYHILYNNVYGIRACALRMTNTYGPRMLVRHARQTALGWFVRQALDNEVISIYGDGEQQRDYTYVDDAVEAFLLAAMN
PAADGQVFNLGGPEPISHLQLISTLTEVAGTGSYRLVPFPPEKARIDIGSVYSDYSRIQAVLGWQPTTSLRQGLEYTVNF
YREFRESYW
>Mature_329_residues
MLEQAFRGKRCLITGGLGFIGSNLAFRLVELGAEVVIVDSLVAEYGGNMANVHGLAEQVRINIADVRDQHSLQYLVQGQD
YLFNLAGQVSHIDSMENPFNDLDINCRAQLSILEACRHNNPAIKIVFASTRQIYGRPDYVPVDEQHLLHPTDVNGINKMA
GEWYHILYNNVYGIRACALRMTNTYGPRMLVRHARQTALGWFVRQALDNEVISIYGDGEQQRDYTYVDDAVEAFLLAAMN
PAADGQVFNLGGPEPISHLQLISTLTEVAGTGSYRLVPFPPEKARIDIGSVYSDYSRIQAVLGWQPTTSLRQGLEYTVNF
YREFRESYW

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=320, Percent_Identity=30.9375, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=25.4601226993865, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=23.8372093023256, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=23.8372093023256, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=23.8372093023256, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=27.2206303724928, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=25.7225433526012, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788589, Length=346, Percent_Identity=21.9653179190751, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=22.0630372492837, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=324, Percent_Identity=28.7037037037037, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17568069, Length=339, Percent_Identity=27.7286135693215, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=338, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=340, Percent_Identity=27.0588235294118, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115532424, Length=333, Percent_Identity=24.6246246246246, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21356223, Length=325, Percent_Identity=28.6153846153846, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 36983; Mature: 36983

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQAFRGKRCLITGGLGFIGSNLAFRLVELGAEVVIVDSLVAEYGGNMANVHGLAEQVR
CCCCHHCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHE
INIADVRDQHSLQYLVQGQDYLFNLAGQVSHIDSMENPFNDLDINCRAQLSILEACRHNN
EEHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
PAIKIVFASTRQIYGRPDYVPVDEQHLLHPTDVNGINKMAGEWYHILYNNVYGIRACALR
CEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
MTNTYGPRMLVRHARQTALGWFVRQALDNEVISIYGDGEQQRDYTYVDDAVEAFLLAAMN
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PAADGQVFNLGGPEPISHLQLISTLTEVAGTGSYRLVPFPPEKARIDIGSVYSDYSRIQA
CCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHEEEHHHHHHHHHHHHH
VLGWQPTTSLRQGLEYTVNFYREFRESYW
HHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEQAFRGKRCLITGGLGFIGSNLAFRLVELGAEVVIVDSLVAEYGGNMANVHGLAEQVR
CCCCHHCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHHHHHHHHE
INIADVRDQHSLQYLVQGQDYLFNLAGQVSHIDSMENPFNDLDINCRAQLSILEACRHNN
EEHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
PAIKIVFASTRQIYGRPDYVPVDEQHLLHPTDVNGINKMAGEWYHILYNNVYGIRACALR
CEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
MTNTYGPRMLVRHARQTALGWFVRQALDNEVISIYGDGEQQRDYTYVDDAVEAFLLAAMN
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PAADGQVFNLGGPEPISHLQLISTLTEVAGTGSYRLVPFPPEKARIDIGSVYSDYSRIQA
CCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHEEEHHHHHHHHHHHHH
VLGWQPTTSLRQGLEYTVNFYREFRESYW
HHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]