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The map label for this gene is iscS [H]

Identifier: 159900399

GI number: 159900399

Start: 4876784

End: 4877980

Strand: Direct

Name: iscS [H]

Synonym: Haur_3884

Alternate gene names: 159900399

Gene position: 4876784-4877980 (Clockwise)

Preceding gene: 159900394

Following gene: 159900400

Centisome position: 76.84

GC content: 53.63

Gene sequence:

>1197_bases
ATGGCTCCGGAAACAATTTATTTAGATCATGCAGCGACGACTGCGACCGATCCAAGGGTGGTTGAGGCCATGCTGCCCTA
CTTCAACACGGCCTATGGCAATCCCTCGAGCATCTATCGGCTGGGCCGCGCAGCGCTCGAAGGCGTAGATGAAGCCCGCG
AAACCGTTGCGAGTTTGCTAGGAGCAAAACGCAAAGAAATTGTGTTTACCAGCGGCGGCTCCGAAGCCGATAATTTGGCG
ATCAAGGGCGTGGCATTTGCTCAGCGTGATGCAGGCAAAGGCAATCACATCATCACCAGTGCCATTGAACATCACGCGGT
GCTGCATGCAGTGGAATATCTCGAACACTTTGGCTTTGAAATCACGATTTTGCCGGTCGATAGCACGGGTTTGGTTGCCG
TGGCCGATTTACGGGCCGCGATTCGCCCAACCACGGTGTTGGTCAGCATTATGGCCGCCAACAACGAGATTGGCACGATT
CAGCCAATTGCCGAATTGGGCGCGGTTTGTCGCGAGCACAATGTGCTGTTTCATACCGATGCCGTGCAGTTGATCGGGGC
GCAACCAATTAATGTTAAAGAATTGAATGTTGATTTGTTGAGCCTAACTGCGCATAAATTTTATGGTCCCAAAGGCGTAG
GCGCGTTGTATATGCGGCGCGGCGTACCCTTGCTACCGTTGATTAATGGTGGCTCACAGGAACGGCGGTTACGCGCTGGC
ACCGAAAATGTGCCTGGGATCGTTGGGCTAGCCAAAGCCTTGCAACTTGCCGTCGATGAATTGCCACAAAGCAGCAACCA
ACTAACCAGCCTGCGCGATCGGCTGATTAGCGGAATTGAGGCAGCAATCCCGCATGTCTATTTAAATGGCCATCGCAGCC
AGCGTTTGCCCAATAATGTCAACATGTCGTTTGATTTTATTGAGGGCGAAAGCATGTTGTTGTTGCTTGATCAGCAGGGC
ATTTATGCCTCGAGTGGCTCGGCCTGCACCAGCGGTTCGCTTGACCCATCGCATGTTCTGATGGCCTTGGGCTTGAGTGC
CGAACGCGCTCATGGCAGCCTGCGCATGACCCTTGGCCGCGAGAACACCGCCGAGCAAATCGAGCGCGTCTTAGCATTGT
TGCCGCCAATCGTCGAGCGCTTGCGGGCAGTTTCGCCGATGTATCGCCATTTCTTGGCGGAACAAACTGTTTATTAA

Upstream 100 bases:

>100_bases
TTTGGCCGAATGTAGCGAAATGTTTACAGCAGGTTGGGGTTAAAAAGCGGTAGAATCAAACCAGCGACAAACTTGCAATT
AGCATCAAAGGTGGTCTGGT

Downstream 100 bases:

>100_bases
GCTTGTCAATCCCTCTCCCCCGCCTCTCTCCTACTGCGGCGGGCAGGGGCATTCCAAGCTTCATAATCGAGGGATTCCGC
CTCGCGTGCGGGAGCGGAGG

Product: class V aminotransferase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAPETIYLDHAATTATDPRVVEAMLPYFNTAYGNPSSIYRLGRAALEGVDEARETVASLLGAKRKEIVFTSGGSEADNLA
IKGVAFAQRDAGKGNHIITSAIEHHAVLHAVEYLEHFGFEITILPVDSTGLVAVADLRAAIRPTTVLVSIMAANNEIGTI
QPIAELGAVCREHNVLFHTDAVQLIGAQPINVKELNVDLLSLTAHKFYGPKGVGALYMRRGVPLLPLINGGSQERRLRAG
TENVPGIVGLAKALQLAVDELPQSSNQLTSLRDRLISGIEAAIPHVYLNGHRSQRLPNNVNMSFDFIEGESMLLLLDQQG
IYASSGSACTSGSLDPSHVLMALGLSAERAHGSLRMTLGRENTAEQIERVLALLPPIVERLRAVSPMYRHFLAEQTVY

Sequences:

>Translated_398_residues
MAPETIYLDHAATTATDPRVVEAMLPYFNTAYGNPSSIYRLGRAALEGVDEARETVASLLGAKRKEIVFTSGGSEADNLA
IKGVAFAQRDAGKGNHIITSAIEHHAVLHAVEYLEHFGFEITILPVDSTGLVAVADLRAAIRPTTVLVSIMAANNEIGTI
QPIAELGAVCREHNVLFHTDAVQLIGAQPINVKELNVDLLSLTAHKFYGPKGVGALYMRRGVPLLPLINGGSQERRLRAG
TENVPGIVGLAKALQLAVDELPQSSNQLTSLRDRLISGIEAAIPHVYLNGHRSQRLPNNVNMSFDFIEGESMLLLLDQQG
IYASSGSACTSGSLDPSHVLMALGLSAERAHGSLRMTLGRENTAEQIERVLALLPPIVERLRAVSPMYRHFLAEQTVY
>Mature_397_residues
APETIYLDHAATTATDPRVVEAMLPYFNTAYGNPSSIYRLGRAALEGVDEARETVASLLGAKRKEIVFTSGGSEADNLAI
KGVAFAQRDAGKGNHIITSAIEHHAVLHAVEYLEHFGFEITILPVDSTGLVAVADLRAAIRPTTVLVSIMAANNEIGTIQ
PIAELGAVCREHNVLFHTDAVQLIGAQPINVKELNVDLLSLTAHKFYGPKGVGALYMRRGVPLLPLINGGSQERRLRAGT
ENVPGIVGLAKALQLAVDELPQSSNQLTSLRDRLISGIEAAIPHVYLNGHRSQRLPNNVNMSFDFIEGESMLLLLDQQGI
YASSGSACTSGSLDPSHVLMALGLSAERAHGSLRMTLGRENTAEQIERVLALLPPIVERLRAVSPMYRHFLAEQTVY

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=45.0777202072539, Blast_Score=341, Evalue=5e-94,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=37.2596153846154, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI48994898, Length=392, Percent_Identity=47.4489795918367, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1789175, Length=389, Percent_Identity=28.5347043701799, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787970, Length=397, Percent_Identity=25.4408060453401, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25143064, Length=390, Percent_Identity=47.6923076923077, Blast_Score=353, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17533177, Length=316, Percent_Identity=33.5443037974684, Blast_Score=160, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=386, Percent_Identity=46.6321243523316, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI20129463, Length=387, Percent_Identity=46.5116279069767, Blast_Score=338, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42901; Mature: 42770

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPETIYLDHAATTATDPRVVEAMLPYFNTAYGNPSSIYRLGRAALEGVDEARETVASLL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAKRKEIVFTSGGSEADNLAIKGVAFAQRDAGKGNHIITSAIEHHAVLHAVEYLEHFGFE
CCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCE
ITILPVDSTGLVAVADLRAAIRPTTVLVSIMAANNEIGTIQPIAELGAVCREHNVLFHTD
EEEEEECCCCCEEHHHHHHHHCHHHHEEEHEECCCCCCCCHHHHHHHHHHHHCCEEEEEC
AVQLIGAQPINVKELNVDLLSLTAHKFYGPKGVGALYMRRGVPLLPLINGGSQERRLRAG
HHHHHCCCCCCEEECCEEHEEHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHCC
TENVPGIVGLAKALQLAVDELPQSSNQLTSLRDRLISGIEAAIPHVYLNGHRSQRLPNNV
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
NMSFDFIEGESMLLLLDQQGIYASSGSACTSGSLDPSHVLMALGLSAERAHGSLRMTLGR
CCEEEEECCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHCCCHHHCCCCEEEEECC
ENTAEQIERVLALLPPIVERLRAVSPMYRHFLAEQTVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
APETIYLDHAATTATDPRVVEAMLPYFNTAYGNPSSIYRLGRAALEGVDEARETVASLL
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAKRKEIVFTSGGSEADNLAIKGVAFAQRDAGKGNHIITSAIEHHAVLHAVEYLEHFGFE
CCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCE
ITILPVDSTGLVAVADLRAAIRPTTVLVSIMAANNEIGTIQPIAELGAVCREHNVLFHTD
EEEEEECCCCCEEHHHHHHHHCHHHHEEEHEECCCCCCCCHHHHHHHHHHHHCCEEEEEC
AVQLIGAQPINVKELNVDLLSLTAHKFYGPKGVGALYMRRGVPLLPLINGGSQERRLRAG
HHHHHCCCCCCEEECCEEHEEHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHCC
TENVPGIVGLAKALQLAVDELPQSSNQLTSLRDRLISGIEAAIPHVYLNGHRSQRLPNNV
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
NMSFDFIEGESMLLLLDQQGIYASSGSACTSGSLDPSHVLMALGLSAERAHGSLRMTLGR
CCEEEEECCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHCCCHHHCCCCEEEEECC
ENTAEQIERVLALLPPIVERLRAVSPMYRHFLAEQTVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]