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The map label for this gene is amtB [C]

Identifier: 159900308

GI number: 159900308

Start: 4760163

End: 4761569

Strand: Reverse

Name: amtB [C]

Synonym: Haur_3791

Alternate gene names: 159900308

Gene position: 4761569-4760163 (Counterclockwise)

Preceding gene: 159900310

Following gene: 159900307

Centisome position: 75.03

GC content: 52.52

Gene sequence:

>1407_bases
ATGTTTCGACGACGACGCTGGATTGTACCCGCAGCAGTTGCTTCGCTAGCAATACCCACTGCAACTTTTGCCCAAGAAGC
CGCCCCGGTAGCAACCGAAACAATCACCACCCAAATTGATATTATTTGGTTGTTGATTGCCGCGGTTTTGGTCTTTTTTA
TGCAGGCTGGCTTTGCCTTGGTCGAAAGTGGCTTTACTCGTTCCAAAAACGCCGCCAACATCTTGATGAAAAACTTGATG
GATTTTTGTGTTGGCACGGTGTTGTTCTTTGCAATTGGCTTCGGCTTGATGTATGGTGCATCAGCTGGTGGCTTCATCGG
TACCGACAACTTCTTCCTCAGCGACTTTAGCCCAGCAACTGGCAGCGGCAAAGATTGGGTTGGTTTCTTCTTCCAATTGG
TGTTTGCCGCCACCGCCGCCACAATTGTGTCGGGCGCAGTCGCCGAACGCTTCAAATTTATCTCCTACCTGATTTATAGC
GCCGTGATTTGTGGCATTGTCTACCCCATTTCGGGACACTGGCAATGGGGCGGCGGTTGGCTATTCCAACTGGGCTTTAT
CGACTTTGCTGGCTCGACGATTGTGCATAGCGTTGGTGGCTGGGCCGCAGTTGCTGGCACGATTTTGCTTGGGCCACGCT
TGGGCAAATTCAACAAAGATGGCTCAAGCAACGTTATTCCAGGCCACAGCTTGACCCTTGGCGTGCTTGGGGTCTTTATT
CTGTGGGTTGGCTGGTTTGGCTTCAACCCAGGTAGCACCCTCTCGGGCATGAACGAAAGCATTGGCTATATTGCCGTCAC
CACCAACATGGCCGCTGCGGTTGGCGCACTCTCAGCCCTGATCACCAACTGGTTGATCACTGGTCACCCCGATACTGCCG
TAGCTTCCAACGGCGCACTCGCTGGTTTGGTCGCAATTACTGCAGGCTGTGCCAATGTTACGCCAGTTGGCGCATTGTTC
ATCGGCTTGCTCGGCGGGATCATCTTCGTCTATGGTTCGATCTTCCTCGAAAAAGTCTTGAAACTTGATGATCCAGTTGG
GGCAATTCCAGTTCACTGTTTCAATGGGATTTTCGGCACCTTGGCAGTTGGCTTGTTTGCTAGCCCGGCTGTTGGCGGCT
TGACTGGCATGGGCGATGCTGCTGGCTTGTTCTATGGCGGTGGTTTGGGCCTACTCGGCACCCAAGCGATTGGGGTTTTG
GCAGTTGGCGCTTGGGCCTTCACAACCATGTTCATCGTCTTCTATGTGCTGAAAAAGACGTTGGGCATTCGGGTATCGGA
GCAAGAAGAAATCGAAGGCTTGGATATTTCTGAGCACGACACGGTTTCATACCCCGAGTTTGGCACGCCGGTTGCTGGTG
TGGCAGGTGCTCGCTCAATGATGCCAGAGCTTTCGTCAACCAAATAA

Upstream 100 bases:

>100_bases
GCATTTGTTAGCTTGATCAACTCAATAGATTCCAACCTATTGCTTGATCGGCGTGTATCGCATCGTAGCCAATCATTACC
TGTCATCAAGGGAGGTTCTA

Downstream 100 bases:

>100_bases
ACCAATTCAAGCATGCGCTGATTGAGCTAATCTCAGTCAGCGCATCGTTAGCATCAAGGAGCAATTATGCAACTTGCAGT
GAGCGCCAAACCTGAAACTC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFRRRRWIVPAAVASLAIPTATFAQEAAPVATETITTQIDIIWLLIAAVLVFFMQAGFALVESGFTRSKNAANILMKNLM
DFCVGTVLFFAIGFGLMYGASAGGFIGTDNFFLSDFSPATGSGKDWVGFFFQLVFAATAATIVSGAVAERFKFISYLIYS
AVICGIVYPISGHWQWGGGWLFQLGFIDFAGSTIVHSVGGWAAVAGTILLGPRLGKFNKDGSSNVIPGHSLTLGVLGVFI
LWVGWFGFNPGSTLSGMNESIGYIAVTTNMAAAVGALSALITNWLITGHPDTAVASNGALAGLVAITAGCANVTPVGALF
IGLLGGIIFVYGSIFLEKVLKLDDPVGAIPVHCFNGIFGTLAVGLFASPAVGGLTGMGDAAGLFYGGGLGLLGTQAIGVL
AVGAWAFTTMFIVFYVLKKTLGIRVSEQEEIEGLDISEHDTVSYPEFGTPVAGVAGARSMMPELSSTK

Sequences:

>Translated_468_residues
MFRRRRWIVPAAVASLAIPTATFAQEAAPVATETITTQIDIIWLLIAAVLVFFMQAGFALVESGFTRSKNAANILMKNLM
DFCVGTVLFFAIGFGLMYGASAGGFIGTDNFFLSDFSPATGSGKDWVGFFFQLVFAATAATIVSGAVAERFKFISYLIYS
AVICGIVYPISGHWQWGGGWLFQLGFIDFAGSTIVHSVGGWAAVAGTILLGPRLGKFNKDGSSNVIPGHSLTLGVLGVFI
LWVGWFGFNPGSTLSGMNESIGYIAVTTNMAAAVGALSALITNWLITGHPDTAVASNGALAGLVAITAGCANVTPVGALF
IGLLGGIIFVYGSIFLEKVLKLDDPVGAIPVHCFNGIFGTLAVGLFASPAVGGLTGMGDAAGLFYGGGLGLLGTQAIGVL
AVGAWAFTTMFIVFYVLKKTLGIRVSEQEEIEGLDISEHDTVSYPEFGTPVAGVAGARSMMPELSSTK
>Mature_468_residues
MFRRRRWIVPAAVASLAIPTATFAQEAAPVATETITTQIDIIWLLIAAVLVFFMQAGFALVESGFTRSKNAANILMKNLM
DFCVGTVLFFAIGFGLMYGASAGGFIGTDNFFLSDFSPATGSGKDWVGFFFQLVFAATAATIVSGAVAERFKFISYLIYS
AVICGIVYPISGHWQWGGGWLFQLGFIDFAGSTIVHSVGGWAAVAGTILLGPRLGKFNKDGSSNVIPGHSLTLGVLGVFI
LWVGWFGFNPGSTLSGMNESIGYIAVTTNMAAAVGALSALITNWLITGHPDTAVASNGALAGLVAITAGCANVTPVGALF
IGLLGGIIFVYGSIFLEKVLKLDDPVGAIPVHCFNGIFGTLAVGLFASPAVGGLTGMGDAAGLFYGGGLGLLGTQAIGVL
AVGAWAFTTMFIVFYVLKKTLGIRVSEQEEIEGLDISEHDTVSYPEFGTPVAGVAGARSMMPELSSTK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=32.9355608591885, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=38.4792626728111, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=36.734693877551, Blast_Score=241, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI32564042, Length=416, Percent_Identity=34.375, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=32.2429906542056, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=28.6046511627907, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=28.125, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=430, Percent_Identity=27.4418604651163, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=38.0487804878049, Blast_Score=224, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 48723; Mature: 48723

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRRRWIVPAAVASLAIPTATFAQEAAPVATETITTQIDIIWLLIAAVLVFFMQAGFAL
CCCCCCEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESGFTRSKNAANILMKNLMDFCVGTVLFFAIGFGLMYGASAGGFIGTDNFFLSDFSPAT
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
GSGKDWVGFFFQLVFAATAATIVSGAVAERFKFISYLIYSAVICGIVYPISGHWQWGGGW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
LFQLGFIDFAGSTIVHSVGGWAAVAGTILLGPRLGKFNKDGSSNVIPGHSLTLGVLGVFI
EEEEHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LWVGWFGFNPGSTLSGMNESIGYIAVTTNMAAAVGALSALITNWLITGHPDTAVASNGAL
HHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCEEECCCCCHHCCCCCH
AGLVAITAGCANVTPVGALFIGLLGGIIFVYGSIFLEKVLKLDDPVGAIPVHCFNGIFGT
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LAVGLFASPAVGGLTGMGDAAGLFYGGGLGLLGTQAIGVLAVGAWAFTTMFIVFYVLKKT
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIRVSEQEEIEGLDISEHDTVSYPEFGTPVAGVAGARSMMPELSSTK
HCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFRRRRWIVPAAVASLAIPTATFAQEAAPVATETITTQIDIIWLLIAAVLVFFMQAGFAL
CCCCCCEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VESGFTRSKNAANILMKNLMDFCVGTVLFFAIGFGLMYGASAGGFIGTDNFFLSDFSPAT
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
GSGKDWVGFFFQLVFAATAATIVSGAVAERFKFISYLIYSAVICGIVYPISGHWQWGGGW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCC
LFQLGFIDFAGSTIVHSVGGWAAVAGTILLGPRLGKFNKDGSSNVIPGHSLTLGVLGVFI
EEEEHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LWVGWFGFNPGSTLSGMNESIGYIAVTTNMAAAVGALSALITNWLITGHPDTAVASNGAL
HHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCEEECCCCCHHCCCCCH
AGLVAITAGCANVTPVGALFIGLLGGIIFVYGSIFLEKVLKLDDPVGAIPVHCFNGIFGT
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LAVGLFASPAVGGLTGMGDAAGLFYGGGLGLLGTQAIGVLAVGAWAFTTMFIVFYVLKKT
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIRVSEQEEIEGLDISEHDTVSYPEFGTPVAGVAGARSMMPELSSTK
HCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]