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The map label for this gene is ycbU [H]

Identifier: 159900299

GI number: 159900299

Start: 4751114

End: 4752268

Strand: Direct

Name: ycbU [H]

Synonym: Haur_3782

Alternate gene names: 159900299

Gene position: 4751114-4752268 (Clockwise)

Preceding gene: 159900298

Following gene: 159900300

Centisome position: 74.86

GC content: 54.29

Gene sequence:

>1155_bases
GTGTTTACAACCATGAGCACTGCACCGCGTGATTGGTTGGCTAGTCGGGCTGAGTTCCCGATTTTGAGCCGCAAAACCTA
TCTGAATACATGTTCGCTTGGCGCATTGAGCAGCCGCGTGCGTGCGGCAGTTAATCAGCATTTGGATTTATGGGAAGAGT
ACGGGGCATCGGCGTGGTATAAGATTTGGCTCGGTGAGTGCGCTGCTTTACGCACGACCACCGAACGCTTATTTAATGCT
CCGGCTGGCTCGGTGGCTTTGGCTCCTAGCGTTGGCGTGGCCCTTTCGGTAATCGCCAGTGCCTTGGATTATCGCCAACG
GCCCAAGGTGATTTCAAGCGTCCTCGATTTTCCAACAATTCCGTATCAATGGTTGGCGCATCCTGAGGTTGAGGTGGTGC
TTTTGCCATCGCCCGATGGCGTGCATGTGCCATTGGAAGCCTACGAGGCTGCAATCGATGAGCGCACGGCCTTGGTGGCA
ACCTCGCATGTCTTTTTTGCCAGTGGCACAATTCAGCCAATTGCTGAAATTGCCAAATTAGCCCATGCCAAAGGCGCATT
ATGCCTGATCGATGGCTATCAGGCGGCGGGGCAAGTGCCAGTCGATGTCGTCGCCAGCGATCTGGATTTCTACATGACTG
GGGGCTTGAAGTGGCTGCTTGGTGGAACGGGCATTGTTGAATGCTATGTCCGCCCCAGCCTGATTCAGCAGCTTAAGCCG
ACGGTTGCTGGCTGGTTTGGTCATGCCAACCAATTTGCCTTCGACCCCAACAACTTTGAGTTTGCCCCCGATGCGCGGCG
CTTTGAAGTTGGCACGCCTGCTTTATCGGCGGTCTATGCTGGCCATGCTGGCATGCAGATCATCCTTGACCATGGCGTGG
CGGCAATTCAACAACGCACCAGCGAACTGGTGGCCTATTTGGTGGCCAAGTTACGCGATGCAGGCTATCAACTAACTATT
TCTGGCGACCCACAGCAGCATGCAGGGATCGTGATGATCCAAAGTGCCGAGCATGCCAAGTTGGTGGCTGCCTTGGCTGA
GCAGGGGATAATCGTTGATCATCGGCCTGGCCATGTGCGGGTATCGCCATTTTTCTATACCTTAGAGACAGAGCTTGATC
TTTTTGTGGAACGCTTGCAGGCTCTTAGCCAGTAA

Upstream 100 bases:

>100_bases
TTCGACACTCCCCTTAAACTGCGCTATAATCCAAACAAGTTTCTAGAGTTGCTGATTCGCGAGCCTCTGATCAGAAATTT
CTGATCGGCAGGTGATATTT

Downstream 100 bases:

>100_bases
TTTGGAATTCAGCATCAATAAGGAGGTTACGTGTCCACTCGCACGATTTTGGACCGTTATCAAACGAGTGCCTCGTATCT
AGCGCGCATTTTACTCCGTT

Product: class V aminotransferase

Products: hydrogen selenide; @ALAN01.txt*l-alanine!; acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MFTTMSTAPRDWLASRAEFPILSRKTYLNTCSLGALSSRVRAAVNQHLDLWEEYGASAWYKIWLGECAALRTTTERLFNA
PAGSVALAPSVGVALSVIASALDYRQRPKVISSVLDFPTIPYQWLAHPEVEVVLLPSPDGVHVPLEAYEAAIDERTALVA
TSHVFFASGTIQPIAEIAKLAHAKGALCLIDGYQAAGQVPVDVVASDLDFYMTGGLKWLLGGTGIVECYVRPSLIQQLKP
TVAGWFGHANQFAFDPNNFEFAPDARRFEVGTPALSAVYAGHAGMQIILDHGVAAIQQRTSELVAYLVAKLRDAGYQLTI
SGDPQQHAGIVMIQSAEHAKLVAALAEQGIIVDHRPGHVRVSPFFYTLETELDLFVERLQALSQ

Sequences:

>Translated_384_residues
MFTTMSTAPRDWLASRAEFPILSRKTYLNTCSLGALSSRVRAAVNQHLDLWEEYGASAWYKIWLGECAALRTTTERLFNA
PAGSVALAPSVGVALSVIASALDYRQRPKVISSVLDFPTIPYQWLAHPEVEVVLLPSPDGVHVPLEAYEAAIDERTALVA
TSHVFFASGTIQPIAEIAKLAHAKGALCLIDGYQAAGQVPVDVVASDLDFYMTGGLKWLLGGTGIVECYVRPSLIQQLKP
TVAGWFGHANQFAFDPNNFEFAPDARRFEVGTPALSAVYAGHAGMQIILDHGVAAIQQRTSELVAYLVAKLRDAGYQLTI
SGDPQQHAGIVMIQSAEHAKLVAALAEQGIIVDHRPGHVRVSPFFYTLETELDLFVERLQALSQ
>Mature_384_residues
MFTTMSTAPRDWLASRAEFPILSRKTYLNTCSLGALSSRVRAAVNQHLDLWEEYGASAWYKIWLGECAALRTTTERLFNA
PAGSVALAPSVGVALSVIASALDYRQRPKVISSVLDFPTIPYQWLAHPEVEVVLLPSPDGVHVPLEAYEAAIDERTALVA
TSHVFFASGTIQPIAEIAKLAHAKGALCLIDGYQAAGQVPVDVVASDLDFYMTGGLKWLLGGTGIVECYVRPSLIQQLKP
TVAGWFGHANQFAFDPNNFEFAPDARRFEVGTPALSAVYAGHAGMQIILDHGVAAIQQRTSELVAYLVAKLRDAGYQLTI
SGDPQQHAGIVMIQSAEHAKLVAALAEQGIIVDHRPGHVRVSPFFYTLETELDLFVERLQALSQ

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=405, Percent_Identity=28.641975308642, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.8.1.7 [C]

Molecular weight: Translated: 41711; Mature: 41711

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTTMSTAPRDWLASRAEFPILSRKTYLNTCSLGALSSRVRAAVNQHLDLWEEYGASAWY
CCCCCCCCCHHHHHCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KIWLGECAALRTTTERLFNAPAGSVALAPSVGVALSVIASALDYRQRPKVISSVLDFPTI
EEHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
PYQWLAHPEVEVVLLPSPDGVHVPLEAYEAAIDERTALVATSHVFFASGTIQPIAEIAKL
CHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHH
AHAKGALCLIDGYQAAGQVPVDVVASDLDFYMTGGLKWLLGGTGIVECYVRPSLIQQLKP
HHCCCCEEEEECCCCCCCCCHHHHHCCCCEEEECCEEEEECCCCEEEEEECHHHHHHHHH
TVAGWFGHANQFAFDPNNFEFAPDARRFEVGTPALSAVYAGHAGMQIILDHGVAAIQQRT
HHHHHHCCCCCEEECCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEEHHHHHHHHHHH
SELVAYLVAKLRDAGYQLTISGDPQQHAGIVMIQSAEHAKLVAALAEQGIIVDHRPGHVR
HHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCEEEECCCCEEE
VSPFFYTLETELDLFVERLQALSQ
EEEEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTTMSTAPRDWLASRAEFPILSRKTYLNTCSLGALSSRVRAAVNQHLDLWEEYGASAWY
CCCCCCCCCHHHHHCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KIWLGECAALRTTTERLFNAPAGSVALAPSVGVALSVIASALDYRQRPKVISSVLDFPTI
EEHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
PYQWLAHPEVEVVLLPSPDGVHVPLEAYEAAIDERTALVATSHVFFASGTIQPIAEIAKL
CHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHH
AHAKGALCLIDGYQAAGQVPVDVVASDLDFYMTGGLKWLLGGTGIVECYVRPSLIQQLKP
HHCCCCEEEEECCCCCCCCCHHHHHCCCCEEEECCEEEEECCCCEEEEEECHHHHHHHHH
TVAGWFGHANQFAFDPNNFEFAPDARRFEVGTPALSAVYAGHAGMQIILDHGVAAIQQRT
HHHHHHCCCCCEEECCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEEHHHHHHHHHHH
SELVAYLVAKLRDAGYQLTISGDPQQHAGIVMIQSAEHAKLVAALAEQGIIVDHRPGHVR
HHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCEEEECCCCEEE
VSPFFYTLETELDLFVERLQALSQ
EEEEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-selenocysteine; reduced acceptor [C]

Specific reaction: L-selenocysteine + reduced acceptor = hydrogen selenide + @ALAN01.txt*l-alanine! + acceptor [C]

General reaction: Elimination of H2S or RSH [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]