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The map label for this gene is asnB [H]

Identifier: 159900227

GI number: 159900227

Start: 4665688

End: 4667577

Strand: Reverse

Name: asnB [H]

Synonym: Haur_3710

Alternate gene names: 159900227

Gene position: 4667577-4665688 (Counterclockwise)

Preceding gene: 159900228

Following gene: 159900226

Centisome position: 73.54

GC content: 52.12

Gene sequence:

>1890_bases
ATGTGTGGAATCTGCGGAATCGTCAGTACAAGCTTAATCGAGCCAAACACTGTTCAGGCCATGAACCAACAATTGATGCA
TCGTGGGCCAGATGGTGCGGGCACTTGGCAAACTGCCCATGCTCAATTGGGTCATCGGCGGTTGGCCATTATCGATTTAG
TCACTGGCGATCAGCCATTCAGCAGCCCCGATGGTGCGTGGCAATTAGTCTTTAATGGCGAGATCTACAATTTTCAGGCC
TTGCGCCAACAATTGCATGGGCTTGGGCATCAATTTCGCACCAACAGCGATACCGAGGTACTGCTGGCCGCCTTGATTGA
ATGGGGCGAGCAGGCTTTTTCGCGTTTGGAAGGCATGTTTGCCTTTGCAGCCTGGCATCAACCAAGCCAAAGTTTATGGC
TGGTGCGTGATAGTTTGGGCAAAAAGCCGCTTTACTATAGCCAAACCAAGCATGGATTAATCTTTGGCTCGGAGATCAAA
GCGCTGCTGCAATACCCTGAGCTTGATCACAGCCTCAATCCACAAGCGCTGATCGCCTATTTGACCTATGGCTATGTGCC
AAATCCGGCCACTTGGTATGCCAACATTCAGCAACTTGCCCCAGGCCATGCCTTGGTTTGGCAAGCGGGCAGCATTCGCG
AATGGCAATGGTGGCAAGCACGCCAGATCGCCCAACGACCACGGCTTAACATCTCAGACAAAGCCGCAATTGAACAAACG
CGCCAGTTGGTGCGAGCCGCAGTTGAGCGACGCTTGATCTGCGATGTGCCATTGGGTGCGTTTTTGAGCGGCGGCTTAGA
TTCAAGCATTGTGGTGGCCGAGGCTCAAGCTTTGTTAGGTCAGCAACTGCACACATTTAGTATTGGCTTTGCTGGTGGTG
GCTGGTACGATGAAAGTTCCTACGCCGAATTGGTTGCCAAACAGCTTGGCACTAAACATCAGTGTTTTATGGTTGAGCCT
GATGCCATCGCCAAATTACCCCAGTTGCTAGCCCACTACGATGAGCCATTTCTCGATTCGTCGGCCTTGCCCATGGCCTT
GCTCAGCCAAATGACCCGTGAACATTGCACGGTGGCCTTATCGGGCGATGGCGGCGACGAGGTGTTTGCTGGCTACGAAC
GCTTTGGCGCAGGCTTATGGACGCAGCGCTATCAGCAATTGCCGCGACCGCTACGCCAAATGCTTGAGCAAACCATTGCC
TTGTTGCCACAAACCAAAGCGAGCAGTCGCTTGGCCCGGCTTAAACGGGTGCTGGCAAAAATTCAATTGCCGCTTGCCCA
GGGCTTTCCGCGCTGGCTGATGGCTTTTACACCCGAAGAATTAGCCGCTTGGGGCTTGCCGGCACTGCAACCTAGCGCCG
CCGAACGCTTTAGCCAAGCAACCCAAGGCGTTACTGATCCGCTGGCTCAATTAATCTGTTATAACCTTGGGAGTTATTTG
CCCGATGATCTGTTGGTCAAGGCCGATCGTATGAGCATGGCCTATGGTTTAGAAGTTCGCTCGCCATTTTTGGATCAGCA
ATTGGTTGAGTGGGCTTTGCAATTGCCAGCCAATTTGCATTGGCGCGGTGGGCGTGGCAAGTGGCTTTTACGCCAAGCCT
ATGCCGAACGCCTGCCAAAAATCATCATCGAACGACCTAAACATGGCTTTGGTGTGCCGCTTGATCAATGGTTTCGCCAA
CAACTCAAGCCAATGCTGCACGATTACTTGCTCTCAACGACCAGCCATGTTCAGCAATGGCTGCCCAAACCAAAACTTGA
GCAACTTTGCCACGCGCATTGGGCTGGTACAATGAACGCTGGACATCAACTTTGGACATTGCTCACCTTAGAATTATGGC
TACAAACTCATCACCAAATGAGGCCATATGCGCACAATTATGTGGATTAA

Upstream 100 bases:

>100_bases
TGCGTCAAACGCTCGGCCAAGCCGCCCGTCAAAAAATCGAGGCCGAATACAGCGTGCAGCGCTATCAACCCACGATTGTG
GCAGCACTCAAGGATTATCT

Downstream 100 bases:

>100_bases
GAATCATCGTTGGCTGAGTTCGTTATTGGTAGCCGTGGTTTGCCTTGGTTTAGGCGGGCTGGCAGGTTTGATGCCGTTGC
GCTATTTGGCAGGCGTAGCC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MCGICGIVSTSLIEPNTVQAMNQQLMHRGPDGAGTWQTAHAQLGHRRLAIIDLVTGDQPFSSPDGAWQLVFNGEIYNFQA
LRQQLHGLGHQFRTNSDTEVLLAALIEWGEQAFSRLEGMFAFAAWHQPSQSLWLVRDSLGKKPLYYSQTKHGLIFGSEIK
ALLQYPELDHSLNPQALIAYLTYGYVPNPATWYANIQQLAPGHALVWQAGSIREWQWWQARQIAQRPRLNISDKAAIEQT
RQLVRAAVERRLICDVPLGAFLSGGLDSSIVVAEAQALLGQQLHTFSIGFAGGGWYDESSYAELVAKQLGTKHQCFMVEP
DAIAKLPQLLAHYDEPFLDSSALPMALLSQMTREHCTVALSGDGGDEVFAGYERFGAGLWTQRYQQLPRPLRQMLEQTIA
LLPQTKASSRLARLKRVLAKIQLPLAQGFPRWLMAFTPEELAAWGLPALQPSAAERFSQATQGVTDPLAQLICYNLGSYL
PDDLLVKADRMSMAYGLEVRSPFLDQQLVEWALQLPANLHWRGGRGKWLLRQAYAERLPKIIIERPKHGFGVPLDQWFRQ
QLKPMLHDYLLSTTSHVQQWLPKPKLEQLCHAHWAGTMNAGHQLWTLLTLELWLQTHHQMRPYAHNYVD

Sequences:

>Translated_629_residues
MCGICGIVSTSLIEPNTVQAMNQQLMHRGPDGAGTWQTAHAQLGHRRLAIIDLVTGDQPFSSPDGAWQLVFNGEIYNFQA
LRQQLHGLGHQFRTNSDTEVLLAALIEWGEQAFSRLEGMFAFAAWHQPSQSLWLVRDSLGKKPLYYSQTKHGLIFGSEIK
ALLQYPELDHSLNPQALIAYLTYGYVPNPATWYANIQQLAPGHALVWQAGSIREWQWWQARQIAQRPRLNISDKAAIEQT
RQLVRAAVERRLICDVPLGAFLSGGLDSSIVVAEAQALLGQQLHTFSIGFAGGGWYDESSYAELVAKQLGTKHQCFMVEP
DAIAKLPQLLAHYDEPFLDSSALPMALLSQMTREHCTVALSGDGGDEVFAGYERFGAGLWTQRYQQLPRPLRQMLEQTIA
LLPQTKASSRLARLKRVLAKIQLPLAQGFPRWLMAFTPEELAAWGLPALQPSAAERFSQATQGVTDPLAQLICYNLGSYL
PDDLLVKADRMSMAYGLEVRSPFLDQQLVEWALQLPANLHWRGGRGKWLLRQAYAERLPKIIIERPKHGFGVPLDQWFRQ
QLKPMLHDYLLSTTSHVQQWLPKPKLEQLCHAHWAGTMNAGHQLWTLLTLELWLQTHHQMRPYAHNYVD
>Mature_629_residues
MCGICGIVSTSLIEPNTVQAMNQQLMHRGPDGAGTWQTAHAQLGHRRLAIIDLVTGDQPFSSPDGAWQLVFNGEIYNFQA
LRQQLHGLGHQFRTNSDTEVLLAALIEWGEQAFSRLEGMFAFAAWHQPSQSLWLVRDSLGKKPLYYSQTKHGLIFGSEIK
ALLQYPELDHSLNPQALIAYLTYGYVPNPATWYANIQQLAPGHALVWQAGSIREWQWWQARQIAQRPRLNISDKAAIEQT
RQLVRAAVERRLICDVPLGAFLSGGLDSSIVVAEAQALLGQQLHTFSIGFAGGGWYDESSYAELVAKQLGTKHQCFMVEP
DAIAKLPQLLAHYDEPFLDSSALPMALLSQMTREHCTVALSGDGGDEVFAGYERFGAGLWTQRYQQLPRPLRQMLEQTIA
LLPQTKASSRLARLKRVLAKIQLPLAQGFPRWLMAFTPEELAAWGLPALQPSAAERFSQATQGVTDPLAQLICYNLGSYL
PDDLLVKADRMSMAYGLEVRSPFLDQQLVEWALQLPANLHWRGGRGKWLLRQAYAERLPKIIIERPKHGFGVPLDQWFRQ
QLKPMLHDYLLSTTSHVQQWLPKPKLEQLCHAHWAGTMNAGHQLWTLLTLELWLQTHHQMRPYAHNYVD

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=407, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI168229252, Length=407, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI168229250, Length=407, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI296010848, Length=383, Percent_Identity=27.154046997389, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1786889, Length=400, Percent_Identity=34.25, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71993933, Length=405, Percent_Identity=29.1358024691358, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=405, Percent_Identity=29.6296296296296, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17560178, Length=392, Percent_Identity=28.8265306122449, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321563, Length=421, Percent_Identity=28.978622327791, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325403, Length=422, Percent_Identity=27.7251184834123, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553209, Length=604, Percent_Identity=26.158940397351, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70944; Mature: 70944

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGICGIVSTSLIEPNTVQAMNQQLMHRGPDGAGTWQTAHAQLGHRRLAIIDLVTGDQPF
CCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCC
SSPDGAWQLVFNGEIYNFQALRQQLHGLGHQFRTNSDTEVLLAALIEWGEQAFSRLEGMF
CCCCCCEEEEECCCEECHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFAAWHQPSQSLWLVRDSLGKKPLYYSQTKHGLIFGSEIKALLQYPELDHSLNPQALIAY
HHHHHCCCCCCEEHHHHHCCCCCCEECCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHH
LTYGYVPNPATWYANIQQLAPGHALVWQAGSIREWQWWQARQIAQRPRLNISDKAAIEQT
HHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RQLVRAAVERRLICDVPLGAFLSGGLDSSIVVAEAQALLGQQLHTFSIGFAGGGWYDESS
HHHHHHHHHCCEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHH
YAELVAKQLGTKHQCFMVEPDAIAKLPQLLAHYDEPFLDSSALPMALLSQMTREHCTVAL
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEE
SGDGGDEVFAGYERFGAGLWTQRYQQLPRPLRQMLEQTIALLPQTKASSRLARLKRVLAK
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IQLPLAQGFPRWLMAFTPEELAAWGLPALQPSAAERFSQATQGVTDPLAQLICYNLGSYL
HHHHHHCCCHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PDDLLVKADRMSMAYGLEVRSPFLDQQLVEWALQLPANLHWRGGRGKWLLRQAYAERLPK
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
IIIERPKHGFGVPLDQWFRQQLKPMLHDYLLSTTSHVQQWLPKPKLEQLCHAHWAGTMNA
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
GHQLWTLLTLELWLQTHHQMRPYAHNYVD
CHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MCGICGIVSTSLIEPNTVQAMNQQLMHRGPDGAGTWQTAHAQLGHRRLAIIDLVTGDQPF
CCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCC
SSPDGAWQLVFNGEIYNFQALRQQLHGLGHQFRTNSDTEVLLAALIEWGEQAFSRLEGMF
CCCCCCEEEEECCCEECHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AFAAWHQPSQSLWLVRDSLGKKPLYYSQTKHGLIFGSEIKALLQYPELDHSLNPQALIAY
HHHHHCCCCCCEEHHHHHCCCCCCEECCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHH
LTYGYVPNPATWYANIQQLAPGHALVWQAGSIREWQWWQARQIAQRPRLNISDKAAIEQT
HHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RQLVRAAVERRLICDVPLGAFLSGGLDSSIVVAEAQALLGQQLHTFSIGFAGGGWYDESS
HHHHHHHHHCCEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHH
YAELVAKQLGTKHQCFMVEPDAIAKLPQLLAHYDEPFLDSSALPMALLSQMTREHCTVAL
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCEEEE
SGDGGDEVFAGYERFGAGLWTQRYQQLPRPLRQMLEQTIALLPQTKASSRLARLKRVLAK
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IQLPLAQGFPRWLMAFTPEELAAWGLPALQPSAAERFSQATQGVTDPLAQLICYNLGSYL
HHHHHHCCCHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PDDLLVKADRMSMAYGLEVRSPFLDQQLVEWALQLPANLHWRGGRGKWLLRQAYAERLPK
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
IIIERPKHGFGVPLDQWFRQQLKPMLHDYLLSTTSHVQQWLPKPKLEQLCHAHWAGTMNA
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
GHQLWTLLTLELWLQTHHQMRPYAHNYVD
CHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]