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The map label for this gene is pgdS [H]

Identifier: 159900165

GI number: 159900165

Start: 4588222

End: 4589397

Strand: Direct

Name: pgdS [H]

Synonym: Haur_3648

Alternate gene names: 159900165

Gene position: 4588222-4589397 (Clockwise)

Preceding gene: 159900164

Following gene: 159900171

Centisome position: 72.29

GC content: 53.32

Gene sequence:

>1176_bases
ATGCCCCGATTGCCAATCCTCGCACGGGATCGTCGGCGCTTGCAGATCGCTGGGTTATTGCTTGGCGCTGGGTTAACTGT
GCCGTTGCTGCTTTGGTGGACGTTTCCCGCAACCTCTCCCGCGCCGCTTGGGGCAACGGCCACACCGCAAATTGTGCGCG
TCGGCGCAACCCTCGATGAGCCAATTCCTGCTGATTGGCAAGCCCCCGTTTTAAACCCCGAACTCGAAGCCCAGGCCTTG
GCTATCCCTGAAACCTTGCCGTTAACCGCTAGTGCGCTATTGTATGATTCGATCTCTGCTGATACGGTCTTTACTACAAC
AATTGCTGGGTTGGTTGCCAGCGAGGAATTGAATTTGCGCGATGGCCCGAGCGTTGATTATTTGCCCATGGCGATTTTGC
TCAACACCACGCCGTTGACAGTAGTTGGCCGATTTGAGGGCTGGCTGCAAGTTGTAACCCCGCAACGAGCGCTTGGTTGG
GTTGATGATAGTTATGTGGCCTTGGCCAGTTCAGCCCAAACCCTGCCCCAAGTTAATCTGCATGCCGACCCAAATCCAGT
TTTAGTGGCGGGATTAACGGTTGAACGAGCTAATGTTCGCTCGAAGCCGCAAACTGAAGCTGAAATTATCACGACCTTGA
GCGCTGAGCATGGGCAAGTCAATTTATTGCAACAACGTGAGGGTTGGTTCAATGTGCGCACCAACGATGGCACTGAGGGC
TGGGTTTCCGCCGAACTGTTACAAGCCGATGCCTATATTTTGCGGCGTGTGCCAACCTTGAGTGCCTCGCCCAACGCGCT
TGAAGCGGTGCGTTTGGCCCGCAAATATGTAGGCTATCCCTATGTTTGGGGCGGCGAAACTCCGCGCGGTGGCTTCGATT
GCTCAGGCTTGGTGCTGTATGTTTATGGCAAATTAGGCATCGATATGCCCCATAGCGCCGCCGAACAATGGACTGGTGGT
TATGGCGAGAAAGTTGCTAGTCGCCGCGATTTAGTGCCTGGCGATATTGTTTTTTTCAAAAATACCTATAAAAAAGGCGT
GAGCCATGTGGGCATTTATGCTGGCAATGGCAAAGTGATTCAGGCGCTCTCCGAGAGTTTAGGCATTCGCGTTTCCGATT
TATCCAATAGCTATTGGAGCAGCCGCTATGTTGGGGCAATTCGGCCATTTCCCTAG

Upstream 100 bases:

>100_bases
CCATGCCCTTCAAGAATTTGGCTTACCATTGCCTGCTGCCGCTTTGCAGCAGCTGAATCAAGCCTATGGAATCATTTTTG
AATAATAAGGATTCGTCAAT

Downstream 100 bases:

>100_bases
TTCAATGCCGATTGTAAATCGCGATAGATCGCCACATTATCAAAAACAATCCCCAAATTGACCAAAGCTTGAGCTACCTC
TGGCCGCACGCCAATTAGGG

Product: NLP/P60 protein

Products: NA

Alternate protein names: Poly-gamma-glutamate depolymerase; PGA depolymerase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MPRLPILARDRRRLQIAGLLLGAGLTVPLLLWWTFPATSPAPLGATATPQIVRVGATLDEPIPADWQAPVLNPELEAQAL
AIPETLPLTASALLYDSISADTVFTTTIAGLVASEELNLRDGPSVDYLPMAILLNTTPLTVVGRFEGWLQVVTPQRALGW
VDDSYVALASSAQTLPQVNLHADPNPVLVAGLTVERANVRSKPQTEAEIITTLSAEHGQVNLLQQREGWFNVRTNDGTEG
WVSAELLQADAYILRRVPTLSASPNALEAVRLARKYVGYPYVWGGETPRGGFDCSGLVLYVYGKLGIDMPHSAAEQWTGG
YGEKVASRRDLVPGDIVFFKNTYKKGVSHVGIYAGNGKVIQALSESLGIRVSDLSNSYWSSRYVGAIRPFP

Sequences:

>Translated_391_residues
MPRLPILARDRRRLQIAGLLLGAGLTVPLLLWWTFPATSPAPLGATATPQIVRVGATLDEPIPADWQAPVLNPELEAQAL
AIPETLPLTASALLYDSISADTVFTTTIAGLVASEELNLRDGPSVDYLPMAILLNTTPLTVVGRFEGWLQVVTPQRALGW
VDDSYVALASSAQTLPQVNLHADPNPVLVAGLTVERANVRSKPQTEAEIITTLSAEHGQVNLLQQREGWFNVRTNDGTEG
WVSAELLQADAYILRRVPTLSASPNALEAVRLARKYVGYPYVWGGETPRGGFDCSGLVLYVYGKLGIDMPHSAAEQWTGG
YGEKVASRRDLVPGDIVFFKNTYKKGVSHVGIYAGNGKVIQALSESLGIRVSDLSNSYWSSRYVGAIRPFP
>Mature_390_residues
PRLPILARDRRRLQIAGLLLGAGLTVPLLLWWTFPATSPAPLGATATPQIVRVGATLDEPIPADWQAPVLNPELEAQALA
IPETLPLTASALLYDSISADTVFTTTIAGLVASEELNLRDGPSVDYLPMAILLNTTPLTVVGRFEGWLQVVTPQRALGWV
DDSYVALASSAQTLPQVNLHADPNPVLVAGLTVERANVRSKPQTEAEIITTLSAEHGQVNLLQQREGWFNVRTNDGTEGW
VSAELLQADAYILRRVPTLSASPNALEAVRLARKYVGYPYVWGGETPRGGFDCSGLVLYVYGKLGIDMPHSAAEQWTGGY
GEKVASRRDLVPGDIVFFKNTYKKGVSHVGIYAGNGKVIQALSESLGIRVSDLSNSYWSSRYVGAIRPFP

Specific function: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA) [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133 [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=113, Percent_Identity=44.2477876106195, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786421, Length=121, Percent_Identity=38.0165289256198, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788501, Length=112, Percent_Identity=38.3928571428571, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788001, Length=112, Percent_Identity=37.5, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 42279; Mature: 42147

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLPILARDRRRLQIAGLLLGAGLTVPLLLWWTFPATSPAPLGATATPQIVRVGATLDE
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCC
PIPADWQAPVLNPELEAQALAIPETLPLTASALLYDSISADTVFTTTIAGLVASEELNLR
CCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
DGPSVDYLPMAILLNTTPLTVVGRFEGWLQVVTPQRALGWVDDSYVALASSAQTLPQVNL
CCCCCCCEEEEEEECCCCEEEEECCCCEEEEECCHHHHCCCCCCHHEECCCCCCCCEEEE
HADPNPVLVAGLTVERANVRSKPQTEAEIITTLSAEHGQVNLLQQREGWFNVRTNDGTEG
CCCCCCEEEEEEEEEECCCCCCCCCHHHEEEEECCCCCCEEEEEECCCEEEEEECCCCCC
WVSAELLQADAYILRRVPTLSASPNALEAVRLARKYVGYPYVWGGETPRGGFDCSGLVLY
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEE
VYGKLGIDMPHSAAEQWTGGYGEKVASRRDLVPGDIVFFKNTYKKGVSHVGIYAGNGKVI
EEECCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHCCCCEEEEEECCCHHH
QALSESLGIRVSDLSNSYWSSRYVGAIRPFP
HHHHHHHCEEEEECCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
PRLPILARDRRRLQIAGLLLGAGLTVPLLLWWTFPATSPAPLGATATPQIVRVGATLDE
CCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCC
PIPADWQAPVLNPELEAQALAIPETLPLTASALLYDSISADTVFTTTIAGLVASEELNLR
CCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCC
DGPSVDYLPMAILLNTTPLTVVGRFEGWLQVVTPQRALGWVDDSYVALASSAQTLPQVNL
CCCCCCCEEEEEEECCCCEEEEECCCCEEEEECCHHHHCCCCCCHHEECCCCCCCCEEEE
HADPNPVLVAGLTVERANVRSKPQTEAEIITTLSAEHGQVNLLQQREGWFNVRTNDGTEG
CCCCCCEEEEEEEEEECCCCCCCCCHHHEEEEECCCCCCEEEEEECCCEEEEEECCCCCC
WVSAELLQADAYILRRVPTLSASPNALEAVRLARKYVGYPYVWGGETPRGGFDCSGLVLY
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEE
VYGKLGIDMPHSAAEQWTGGYGEKVASRRDLVPGDIVFFKNTYKKGVSHVGIYAGNGKVI
EEECCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHCCCCEEEEEECCCHHH
QALSESLGIRVSDLSNSYWSSRYVGAIRPFP
HHHHHHHCEEEEECCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12073126; 12644511; 9353933; 9384377; 10658653 [H]