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The map label for this gene is truC [H]

Identifier: 159900009

GI number: 159900009

Start: 4397666

End: 4399348

Strand: Reverse

Name: truC [H]

Synonym: Haur_3492

Alternate gene names: 159900009

Gene position: 4399348-4397666 (Counterclockwise)

Preceding gene: 159900010

Following gene: 159900008

Centisome position: 69.32

GC content: 53.71

Gene sequence:

>1683_bases
ATGCACATCGAAATTCTCTACCGCGACGAGCAAATCCTGGTGATCAATAAACCAACCGGGGTGGCAACCCACGCACCCCA
AGGCGATTTGGCATTAACCGATGTTGAACGCGCTTTGCGTGCCCAATTGCAACTTGAGTATTTGGCGATTCATCAGCGGC
TAGATCGCGATACCTCGGGCGTGATGCTGTTTGCGCTTGACCCCGCCGCCAATGCCAATTTGGCCACGGCCTTTGCTGAA
CATACGATTGAGAAAACCTACCAAGCCTTGGTGTATGGCGTGCCCATCCAAACTCAAGGGGTCATCGATGCTGCGCTTGC
GCCTGCTGGCGATGGCATGATCCAGGTTGCCTCAGCCCATGATCGGCGTGCTCAATCAGCAATTACGCATTATCGGGTCT
TGGTCAGCAGTCCTGATCAGCGATTTAGCTTGTTGGAATTACAGCCAAAAACTGGCCGAACTCACCAATTGCGGGTGCAC
TGCGAATGTTTGGGCCATCCAATTGTTGGCGATCCGCTGTATGATGTGGCGCGAGCTGCCCCCCGACTGATGCTGCATGC
CAGCGAATTACGCTTCACCCACCCGCTTACTCAACAACCATTGCATATTCAAGCGCCAACTCCAGCCTTATTCACGCGGG
TGGCGCAAGGCTTGCCCGAATTACAACAAAGCACCGAGCTAGCTGCGCTGAATGGCTTAATCGAGTTAGCGGCTGAACGG
CGGGCTGTCCTGGCAGCCGATCCTGCTACGACGATCTTTCGGGTGTTTCATGGCCCAAGCGATGGTCTGACCCATCCATG
GTTGCAGCATTGGACGGTCGATAAACTTGATCAGGTATTAATTGCCTCGTGCTATGACGAACATGTGCGCCAAGTGCCAG
CCAGCTTAATCAACGCCTTGGTTGCGCAATGGCAGCCGCAGGCGATTTATGCTAAATATCGCCCTCGGGCTGCTGCCAAA
GTTGACGAGGCCGCGATGGCTGAGTTAGCTCCAACTAGGCCAGTGTGGGGTGAGCCAATCGAGCAAGTGGTGGTGCAAGA
AGCTGGGTTAAGCTATGAATTGCGGCCTAACGACGGCTTGAGCATTGGTTTATATGCTGATATGCGTGAAACTCGCCAAC
GGGTGCGCAATTTGCTTGCCAAGCGTCAATTGCGGGTGCTCAACACCTTTGCCTATACCTGTGGCTTTGGGGTGGCAGCC
GTTGCCGATGCCCCTGAGGCAATCGTGACCAATCTTGATCTTTCGCGGCGCTCGTTGGATTGGGGCAAAATTAATTATGG
CCTGAATCAGTTGGCCGTTGAAGATCGTCAGTTTGTATTTGGCGATGTCTTCGATTGGCTCAGTCGTTGGGTGCGTCAAG
GCCGTCAATTCGATGTGGTGATTCTTGATCCACCGTCGTTTGCCCGCAATCGGGGTAAGCGTTGGCGAGCCGAAGAAGAT
TACGCCGATTTGGTAGCCTTGGCGGTGCAGTTGTTGCCAGCCGATGGCCATTTAATCGCTTGCTGTAACCATGTTGGGCT
TTCGCGGCGGCAATTTCGTGGTCAAGTCGAACGCGGTATGCAGCAAGGGCGTTGGCATGGCACGATTGAAGCCAATTATC
CGGCCTCGCCCTTAGATTACCCCGCTGCCTATGGCGAAAGCCACTTGAAAATTATTTTAGCGACTGGTCAAACCAACGAT
TAA

Upstream 100 bases:

>100_bases
AGCGCTTCGGCGAAACACTGACTGAACAGATGAAGGATGAGGGATGAGGGTATACTATTCCTAATTCCCCGCAGCAATTG
ATTGAAGGAATGAAATCATA

Downstream 100 bases:

>100_bases
TCCTGCTTTAAACTTCTGATGTATAGTAGAGAGCGGAGTATTTTGCTGTTTAAGGTCGGAGCGCAATGGAACGTCAACAA
GTGCAAGTTCGTTTGGCCAG

Product: pseudouridine synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MHIEILYRDEQILVINKPTGVATHAPQGDLALTDVERALRAQLQLEYLAIHQRLDRDTSGVMLFALDPAANANLATAFAE
HTIEKTYQALVYGVPIQTQGVIDAALAPAGDGMIQVASAHDRRAQSAITHYRVLVSSPDQRFSLLELQPKTGRTHQLRVH
CECLGHPIVGDPLYDVARAAPRLMLHASELRFTHPLTQQPLHIQAPTPALFTRVAQGLPELQQSTELAALNGLIELAAER
RAVLAADPATTIFRVFHGPSDGLTHPWLQHWTVDKLDQVLIASCYDEHVRQVPASLINALVAQWQPQAIYAKYRPRAAAK
VDEAAMAELAPTRPVWGEPIEQVVVQEAGLSYELRPNDGLSIGLYADMRETRQRVRNLLAKRQLRVLNTFAYTCGFGVAA
VADAPEAIVTNLDLSRRSLDWGKINYGLNQLAVEDRQFVFGDVFDWLSRWVRQGRQFDVVILDPPSFARNRGKRWRAEED
YADLVALAVQLLPADGHLIACCNHVGLSRRQFRGQVERGMQQGRWHGTIEANYPASPLDYPAAYGESHLKIILATGQTND

Sequences:

>Translated_560_residues
MHIEILYRDEQILVINKPTGVATHAPQGDLALTDVERALRAQLQLEYLAIHQRLDRDTSGVMLFALDPAANANLATAFAE
HTIEKTYQALVYGVPIQTQGVIDAALAPAGDGMIQVASAHDRRAQSAITHYRVLVSSPDQRFSLLELQPKTGRTHQLRVH
CECLGHPIVGDPLYDVARAAPRLMLHASELRFTHPLTQQPLHIQAPTPALFTRVAQGLPELQQSTELAALNGLIELAAER
RAVLAADPATTIFRVFHGPSDGLTHPWLQHWTVDKLDQVLIASCYDEHVRQVPASLINALVAQWQPQAIYAKYRPRAAAK
VDEAAMAELAPTRPVWGEPIEQVVVQEAGLSYELRPNDGLSIGLYADMRETRQRVRNLLAKRQLRVLNTFAYTCGFGVAA
VADAPEAIVTNLDLSRRSLDWGKINYGLNQLAVEDRQFVFGDVFDWLSRWVRQGRQFDVVILDPPSFARNRGKRWRAEED
YADLVALAVQLLPADGHLIACCNHVGLSRRQFRGQVERGMQQGRWHGTIEANYPASPLDYPAAYGESHLKIILATGQTND
>Mature_560_residues
MHIEILYRDEQILVINKPTGVATHAPQGDLALTDVERALRAQLQLEYLAIHQRLDRDTSGVMLFALDPAANANLATAFAE
HTIEKTYQALVYGVPIQTQGVIDAALAPAGDGMIQVASAHDRRAQSAITHYRVLVSSPDQRFSLLELQPKTGRTHQLRVH
CECLGHPIVGDPLYDVARAAPRLMLHASELRFTHPLTQQPLHIQAPTPALFTRVAQGLPELQQSTELAALNGLIELAAER
RAVLAADPATTIFRVFHGPSDGLTHPWLQHWTVDKLDQVLIASCYDEHVRQVPASLINALVAQWQPQAIYAKYRPRAAAK
VDEAAMAELAPTRPVWGEPIEQVVVQEAGLSYELRPNDGLSIGLYADMRETRQRVRNLLAKRQLRVLNTFAYTCGFGVAA
VADAPEAIVTNLDLSRRSLDWGKINYGLNQLAVEDRQFVFGDVFDWLSRWVRQGRQFDVVILDPPSFARNRGKRWRAEED
YADLVALAVQLLPADGHLIACCNHVGLSRRQFRGQVERGMQQGRWHGTIEANYPASPLDYPAAYGESHLKIILATGQTND

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316667, Length=216, Percent_Identity=35.1851851851852, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI221316663, Length=235, Percent_Identity=34.8936170212766, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI23308689, Length=181, Percent_Identity=32.5966850828729, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI17158025, Length=228, Percent_Identity=31.140350877193, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1789155, Length=222, Percent_Identity=39.1891891891892, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1787327, Length=231, Percent_Identity=35.9307359307359, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI145693111, Length=278, Percent_Identity=30.9352517985612, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786244, Length=208, Percent_Identity=36.0576923076923, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788946, Length=239, Percent_Identity=29.7071129707113, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17534965, Length=180, Percent_Identity=32.7777777777778, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320168, Length=174, Percent_Identity=31.6091954022989, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324506, Length=180, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583449, Length=177, Percent_Identity=31.638418079096, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583447, Length=177, Percent_Identity=31.638418079096, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583445, Length=177, Percent_Identity=31.638418079096, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921090, Length=177, Percent_Identity=31.638418079096, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 62275; Mature: 62275

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIEILYRDEQILVINKPTGVATHAPQGDLALTDVERALRAQLQLEYLAIHQRLDRDTSG
CEEEEEEECCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VMLFALDPAANANLATAFAEHTIEKTYQALVYGVPIQTQGVIDAALAPAGDGMIQVASAH
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECHH
DRRAQSAITHYRVLVSSPDQRFSLLELQPKTGRTHQLRVHCECLGHPIVGDPLYDVARAA
HHHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
PRLMLHASELRFTHPLTQQPLHIQAPTPALFTRVAQGLPELQQSTELAALNGLIELAAER
HHHEEEHHHHEECCCCCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
RAVLAADPATTIFRVFHGPSDGLTHPWLQHWTVDKLDQVLIASCYDEHVRQVPASLINAL
CEEEECCCHHHEEHEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAQWQPQAIYAKYRPRAAAKVDEAAMAELAPTRPVWGEPIEQVVVQEAGLSYELRPNDGL
HHCCCCCEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCC
SIGLYADMRETRQRVRNLLAKRQLRVLNTFAYTCGFGVAAVADAPEAIVTNLDLSRRSLD
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHCCCCCCCCCCC
WGKINYGLNQLAVEDRQFVFGDVFDWLSRWVRQGRQFDVVILDPPSFARNRGKRWRAEED
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCHH
YADLVALAVQLLPADGHLIACCNHVGLSRRQFRGQVERGMQQGRWHGTIEANYPASPLDY
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
PAAYGESHLKIILATGQTND
CCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MHIEILYRDEQILVINKPTGVATHAPQGDLALTDVERALRAQLQLEYLAIHQRLDRDTSG
CEEEEEEECCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VMLFALDPAANANLATAFAEHTIEKTYQALVYGVPIQTQGVIDAALAPAGDGMIQVASAH
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECHH
DRRAQSAITHYRVLVSSPDQRFSLLELQPKTGRTHQLRVHCECLGHPIVGDPLYDVARAA
HHHHHHHHHHEEEEECCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
PRLMLHASELRFTHPLTQQPLHIQAPTPALFTRVAQGLPELQQSTELAALNGLIELAAER
HHHEEEHHHHEECCCCCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
RAVLAADPATTIFRVFHGPSDGLTHPWLQHWTVDKLDQVLIASCYDEHVRQVPASLINAL
CEEEECCCHHHEEHEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAQWQPQAIYAKYRPRAAAKVDEAAMAELAPTRPVWGEPIEQVVVQEAGLSYELRPNDGL
HHCCCCCEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCC
SIGLYADMRETRQRVRNLLAKRQLRVLNTFAYTCGFGVAAVADAPEAIVTNLDLSRRSLD
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHCCCCCCCCCCC
WGKINYGLNQLAVEDRQFVFGDVFDWLSRWVRQGRQFDVVILDPPSFARNRGKRWRAEED
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCHH
YADLVALAVQLLPADGHLIACCNHVGLSRRQFRGQVERGMQQGRWHGTIEANYPASPLDY
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
PAAYGESHLKIILATGQTND
CCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]