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The map label for this gene is panB

Identifier: 159899888

GI number: 159899888

Start: 4251201

End: 4252067

Strand: Reverse

Name: panB

Synonym: Haur_3371

Alternate gene names: 159899888

Gene position: 4252067-4251201 (Counterclockwise)

Preceding gene: 159899889

Following gene: 159899882

Centisome position: 67.0

GC content: 53.17

Gene sequence:

>867_bases
ATGCGTACACAATTGAGCGATATTCAACGGATGAAAGATCGGGGCGAACGCTTGGTGATGGTTACGGCCTACGATTACAC
CAGTGCCCAACTGGTCGATCGCACCGATATTCCCTTAATTTTGGTTGGCGATTCGCTGGGGATGGTGATTCAGGGCAATA
GTTCAACTGTGCCAGTAACGATCGATGAAATGGTTTATCACACCAAAGCTGTGATGCGGGGCAGCAGCAAAGCCCTAGTT
GTTGGCGATTTGCCATTTTTGAGCTACACCAGCCCTGAACAGGCCATCCAAAATGCTGGCAAATTGATGCAAATTGGTGG
TTGTGGCGCGGTAAAGCTCGAAGGTGGCGTGAATCTGGCTCCCACCGTGCGCCGCTTGGTCGATTTGGGCATTCCGGTGA
TGGGCCATTTGGGTTTTACCCCACAATCAGTTAATCAAATTGGCATGCGGGTGCAGGGCAAAACCGTGGCCCATGCCCGT
CAGTTGCTTGACGATGCCTTGGCGCTTGAAGCGGCTGGAGCCTTTGCGATTGTGTTGGAGTTAATTCCAGCAGCCTTGGC
CAAGGCGATCACGGCTCGCTTGCATATTCCGACGATTGGCATTGGGGCGGGGGTCGGTTGTGATGGCGAAGTGCAAGTTT
GGCACGATATTTTGGGCTTGTATAGCGATCATCTGCCGCGCCACACCAAACGCTATGCCGATTTGGGCAGCACAATTGAG
CAAGCGCTGACGCAATATGCCCACGAAGTCCGCGAACGCAGTTTCCCAACTGCCGCTAATAGTAGCGTAATTGATGCCAA
TACCTTGGCCGAAGCGCTGGGCGAACAAACCGTCAGCGTTGGAGCAAGTGCCAAAGTCGCTGTCTAA

Upstream 100 bases:

>100_bases
ATCCCTCCCCTCGCTCGCCGCAGTGGGAGAGGGGTTGGGGGTGAGGGAACCCAATCTATTTTCGATAGTTCAACATATGC
AACCAAGCAAAGGAGCTACC

Downstream 100 bases:

>100_bases
ATAAGCGTTAAATCAAATGTCCGCTGATTAATCCCAGCGGACATTTGTTGTTTAATTGAATAATTCAGCGACTGATTCAA
TAATTGCGGTTGGGCGATAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRTQLSDIQRMKDRGERLVMVTAYDYTSAQLVDRTDIPLILVGDSLGMVIQGNSSTVPVTIDEMVYHTKAVMRGSSKALV
VGDLPFLSYTSPEQAIQNAGKLMQIGGCGAVKLEGGVNLAPTVRRLVDLGIPVMGHLGFTPQSVNQIGMRVQGKTVAHAR
QLLDDALALEAAGAFAIVLELIPAALAKAITARLHIPTIGIGAGVGCDGEVQVWHDILGLYSDHLPRHTKRYADLGSTIE
QALTQYAHEVRERSFPTAANSSVIDANTLAEALGEQTVSVGASAKVAV

Sequences:

>Translated_288_residues
MRTQLSDIQRMKDRGERLVMVTAYDYTSAQLVDRTDIPLILVGDSLGMVIQGNSSTVPVTIDEMVYHTKAVMRGSSKALV
VGDLPFLSYTSPEQAIQNAGKLMQIGGCGAVKLEGGVNLAPTVRRLVDLGIPVMGHLGFTPQSVNQIGMRVQGKTVAHAR
QLLDDALALEAAGAFAIVLELIPAALAKAITARLHIPTIGIGAGVGCDGEVQVWHDILGLYSDHLPRHTKRYADLGSTIE
QALTQYAHEVRERSFPTAANSSVIDANTLAEALGEQTVSVGASAKVAV
>Mature_288_residues
MRTQLSDIQRMKDRGERLVMVTAYDYTSAQLVDRTDIPLILVGDSLGMVIQGNSSTVPVTIDEMVYHTKAVMRGSSKALV
VGDLPFLSYTSPEQAIQNAGKLMQIGGCGAVKLEGGVNLAPTVRRLVDLGIPVMGHLGFTPQSVNQIGMRVQGKTVAHAR
QLLDDALALEAAGAFAIVLELIPAALAKAITARLHIPTIGIGAGVGCDGEVQVWHDILGLYSDHLPRHTKRYADLGSTIE
QALTQYAHEVRERSFPTAANSSVIDANTLAEALGEQTVSVGASAKVAV

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=260, Percent_Identity=46.5384615384615, Blast_Score=228, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319653, Length=261, Percent_Identity=35.632183908046, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_HERA2 (A9B2U4)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546135.1
- ProteinModelPortal:   A9B2U4
- SMR:   A9B2U4
- GeneID:   5735232
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3371
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   HAUR316274:HAUR_3371-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30576; Mature: 30576

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 83-83 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTQLSDIQRMKDRGERLVMVTAYDYTSAQLVDRTDIPLILVGDSLGMVIQGNSSTVPVT
CCCHHHHHHHHHHCCCEEEEEEEECCCCHHEECCCCCCEEEECCCCCEEEECCCCEEEEE
IDEMVYHTKAVMRGSSKALVVGDLPFLSYTSPEQAIQNAGKLMQIGGCGAVKLEGGVNLA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCEEEECCCCCHH
PTVRRLVDLGIPVMGHLGFTPQSVNQIGMRVQGKTVAHARQLLDDALALEAAGAFAIVLE
HHHHHHHHHCCCHHHCCCCCHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIPAALAKAITARLHIPTIGIGAGVGCDGEVQVWHDILGLYSDHLPRHTKRYADLGSTIE
HHHHHHHHHHHHHEECCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QALTQYAHEVRERSFPTAANSSVIDANTLAEALGEQTVSVGASAKVAV
HHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MRTQLSDIQRMKDRGERLVMVTAYDYTSAQLVDRTDIPLILVGDSLGMVIQGNSSTVPVT
CCCHHHHHHHHHHCCCEEEEEEEECCCCHHEECCCCCCEEEECCCCCEEEECCCCEEEEE
IDEMVYHTKAVMRGSSKALVVGDLPFLSYTSPEQAIQNAGKLMQIGGCGAVKLEGGVNLA
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEECCCCEEEECCCCCHH
PTVRRLVDLGIPVMGHLGFTPQSVNQIGMRVQGKTVAHARQLLDDALALEAAGAFAIVLE
HHHHHHHHHCCCHHHCCCCCHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIPAALAKAITARLHIPTIGIGAGVGCDGEVQVWHDILGLYSDHLPRHTKRYADLGSTIE
HHHHHHHHHHHHHEECCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QALTQYAHEVRERSFPTAANSSVIDANTLAEALGEQTVSVGASAKVAV
HHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA