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The map label for this gene is cysK [H]

Identifier: 159899851

GI number: 159899851

Start: 4202511

End: 4203440

Strand: Reverse

Name: cysK [H]

Synonym: Haur_3334

Alternate gene names: 159899851

Gene position: 4203440-4202511 (Counterclockwise)

Preceding gene: 159899854

Following gene: 159899846

Centisome position: 66.23

GC content: 53.66

Gene sequence:

>930_bases
ATGGCGCGGATTTATGACAACATTACCCAACTTATCGGCAACACGCCGCTGGTGCGCTTGGGCAAAGTTAATACCACGGG
GGCGACGGTATTAGCTAAGCTTGAGTTCTTCAACCCAGCCAGCAGCGTCAAAGATCGGATTGGCTTGGCGATGATCGAGG
CAGCGGAGGCCGCCGGTCTGATCAATCCAAACGATACCACGATTATCGAGCCAACCAGTGGCAATACGGGGATTGGCCTC
GCATTTGTGGCTGCCGCGAAAGGCTACCGCATTGTGCTGACCATGCCCGAAACCATGAGCTTGGAACGGCGCAAATTACT
CAAAGGCTTTGGAGCCGAATTAGTTTTGACTCCAGGCTCCGAGGGCATGCCTGGGGCAATTCGTCGTGCCGAAGAATTGG
CCGCCGAAAATCCAGGCAGCTTTATTCCGCAACAATTCAAAAATAAAGCCAACCCAGCGGTTCACCAACGCACCACCGCT
GAAGAAATTTGGAATGATACCGATGGCGCTGTCGATATTTTGGTGGCCGGGGTTGGGACTGGTGGCACAATCACTGGGGT
TGCTTCGGTGCTCAAAGAACGCAAGCCAGGCTTTAAGGCAATTGCGGTCGAGCCAACTGCCTCGCCAGTCCTTTCTGGGG
GCAAAATGGGGCCGCACAAAATCCAAGGGATCGGTGCTGGCTTTGTGCCCGACGTGCTTGATACCAGCGTAATTGATGAA
ATTATTCAAGTTACCAACGAACATGCCTTTGAATGGGCACGCAAATTGGCTCACGAAGAAGGCTTGATGGTGGGAATTAG
CTCAGGGGCCGCCGCATGGGCCGCCTTGCAAGTAGCAGCCCGCCCTGAAAATGCTGGCAAAACGATTGTCTTTATTGTGC
CAAGCAACGGCGAACGCTACCTGAGCACGCCATTGTTTGATGCTGAGTAG

Upstream 100 bases:

>100_bases
TCCTTTCTGTGCGCCTTGCCACATTGACACAAACCTAAGCTATGTTATGCTATTATTGTTAGGTTTGTCGTTTAACTTAG
TTAACAAAAGGACCCAAACC

Downstream 100 bases:

>100_bases
AATTTAGGGGTCAGGGGCTAGTTGTCAGAGTCCAGAAAAAGCTAGAAGTAATGTTAACGCAGAGGCGCAGAGTCTTGATA
AGGATGAAGGCGGTTTCAAC

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MARIYDNITQLIGNTPLVRLGKVNTTGATVLAKLEFFNPASSVKDRIGLAMIEAAEAAGLINPNDTTIIEPTSGNTGIGL
AFVAAAKGYRIVLTMPETMSLERRKLLKGFGAELVLTPGSEGMPGAIRRAEELAAENPGSFIPQQFKNKANPAVHQRTTA
EEIWNDTDGAVDILVAGVGTGGTITGVASVLKERKPGFKAIAVEPTASPVLSGGKMGPHKIQGIGAGFVPDVLDTSVIDE
IIQVTNEHAFEWARKLAHEEGLMVGISSGAAAWAALQVAARPENAGKTIVFIVPSNGERYLSTPLFDAE

Sequences:

>Translated_309_residues
MARIYDNITQLIGNTPLVRLGKVNTTGATVLAKLEFFNPASSVKDRIGLAMIEAAEAAGLINPNDTTIIEPTSGNTGIGL
AFVAAAKGYRIVLTMPETMSLERRKLLKGFGAELVLTPGSEGMPGAIRRAEELAAENPGSFIPQQFKNKANPAVHQRTTA
EEIWNDTDGAVDILVAGVGTGGTITGVASVLKERKPGFKAIAVEPTASPVLSGGKMGPHKIQGIGAGFVPDVLDTSVIDE
IIQVTNEHAFEWARKLAHEEGLMVGISSGAAAWAALQVAARPENAGKTIVFIVPSNGERYLSTPLFDAE
>Mature_308_residues
ARIYDNITQLIGNTPLVRLGKVNTTGATVLAKLEFFNPASSVKDRIGLAMIEAAEAAGLINPNDTTIIEPTSGNTGIGLA
FVAAAKGYRIVLTMPETMSLERRKLLKGFGAELVLTPGSEGMPGAIRRAEELAAENPGSFIPQQFKNKANPAVHQRTTAE
EIWNDTDGAVDILVAGVGTGGTITGVASVLKERKPGFKAIAVEPTASPVLSGGKMGPHKIQGIGAGFVPDVLDTSVIDEI
IQVTNEHAFEWARKLAHEEGLMVGISSGAAAWAALQVAARPENAGKTIVFIVPSNGERYLSTPLFDAE

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=316, Percent_Identity=43.6708860759494, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI295821200, Length=316, Percent_Identity=43.6708860759494, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4557415, Length=316, Percent_Identity=43.6708860759494, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=54.2857142857143, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI2367138, Length=303, Percent_Identity=41.2541254125413, Blast_Score=212, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=52.3489932885906, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=57.7049180327869, Blast_Score=312, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17562970, Length=296, Percent_Identity=49.3243243243243, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI32566674, Length=291, Percent_Identity=49.8281786941581, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17561720, Length=296, Percent_Identity=47.2972972972973, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=50.4716981132076, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17534315, Length=321, Percent_Identity=38.9408099688474, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI25147552, Length=320, Percent_Identity=35.9375, Blast_Score=160, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17561716, Length=116, Percent_Identity=49.1379310344828, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321594, Length=321, Percent_Identity=39.5638629283489, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6321449, Length=325, Percent_Identity=36.9230769230769, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24643623, Length=316, Percent_Identity=39.2405063291139, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI20129101, Length=316, Percent_Identity=39.2405063291139, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32451; Mature: 32319

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIYDNITQLIGNTPLVRLGKVNTTGATVLAKLEFFNPASSVKDRIGLAMIEAAEAAGL
CCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
INPNDTTIIEPTSGNTGIGLAFVAAAKGYRIVLTMPETMSLERRKLLKGFGAELVLTPGS
CCCCCCEEEECCCCCCCCHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
EGMPGAIRRAEELAAENPGSFIPQQFKNKANPAVHQRTTAEEIWNDTDGAVDILVAGVGT
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHCCHHHHHCCCCCCEEEEEEECCC
GGTITGVASVLKERKPGFKAIAVEPTASPVLSGGKMGPHKIQGIGAGFVPDVLDTSVIDE
CCHHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCHHEEECCCCCCHHHHHHHHHHH
IIQVTNEHAFEWARKLAHEEGLMVGISSGAAAWAALQVAARPENAGKTIVFIVPSNGERY
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHEEECCCCCCCEEEEEECCCCCEE
LSTPLFDAE
ECCCCCCCC
>Mature Secondary Structure 
ARIYDNITQLIGNTPLVRLGKVNTTGATVLAKLEFFNPASSVKDRIGLAMIEAAEAAGL
CHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
INPNDTTIIEPTSGNTGIGLAFVAAAKGYRIVLTMPETMSLERRKLLKGFGAELVLTPGS
CCCCCCEEEECCCCCCCCHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCC
EGMPGAIRRAEELAAENPGSFIPQQFKNKANPAVHQRTTAEEIWNDTDGAVDILVAGVGT
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHCCHHHHHCCCCCCEEEEEEECCC
GGTITGVASVLKERKPGFKAIAVEPTASPVLSGGKMGPHKIQGIGAGFVPDVLDTSVIDE
CCHHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCCCCCHHEEECCCCCCHHHHHHHHHHH
IIQVTNEHAFEWARKLAHEEGLMVGISSGAAAWAALQVAARPENAGKTIVFIVPSNGERY
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHEEECCCCCCCEEEEEECCCCCEE
LSTPLFDAE
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]