Click here to switch to the map view.

The map label for this gene is fabI [H]

Identifier: 159899778

GI number: 159899778

Start: 4122146

End: 4122934

Strand: Reverse

Name: fabI [H]

Synonym: Haur_3260

Alternate gene names: 159899778

Gene position: 4122934-4122146 (Counterclockwise)

Preceding gene: 159899779

Following gene: 159899777

Centisome position: 64.96

GC content: 54.25

Gene sequence:

>789_bases
ATGGATTTGCTCAAAGGGAAAAAAGCCTTAATCGTTGGAATTGCCAATGATCATTCAATTGCTTGGGGAATTGCCCAAGC
CTTGAAACAGGCTGGAGCCGAATTGGCCTTTTCGTATGCTGGCGAGAGCTTAGAACGCCGCGTGCGCCCATTGGCCGAAC
AGCTTGGTGTGAGTTTTGTGCAGCAATGCGATGTGAGCGACGATGCCCAAATTGCCACGTTGTTCGAGCGGCTGGGAGCT
GAATTTGGCTCGTTCGATATTTTGGTGCACAGCGTGGCCTACGCCAAACGCGAAGATCTTTCGGGCCGTTTTGTCGATAG
TAGCCGCGATGGCTGGCGGATTGCGATGGATGTCAGTGCTTTTTCGTTGGTAGCGCTCACCAAGGCCGCCATGCCATTGC
TCAATCCTAACGCCAGTATTTTGACCTTGAGCTACTACGGCGCAGAGAAGGTTATTCCTAACTATAACGTGATGGGTGTG
GCCAAAGCCGCACTTGAGGCCACAGTCCGCTATTTGGCCGCCGATCTTGGCCCCGATGGGTTGCGGGTCAATGCGATTAG
TGCTGGGCCAATCCGCACGCTTTCGGCTTCGGGCATTGGCAACTTCCGGCGGTTGCACCGCGCCTTTACCAACGCCAACC
CCATGCGTCGTGCGATCACGATTGAAGATGTTGGCAATAGCGCTTTGTGGCTGTGCTCGGAGCTAGCCAGCGGCGTAACT
GGCGAGGTCGTCTATGTCGATGGTGGCTATAACGTGCTCGGCATGAGCGAAGAAGAACAATCAAGCTAA

Upstream 100 bases:

>100_bases
GTCATTCAGGGGAGATTGACAAGTTTTGTCATATATTTGCCGTTGAGGTTTGTTACAGTGGTGCTGTGCCACTGAACAAT
GTGCTAGTTCAGGAGACACA

Downstream 100 bases:

>100_bases
CAGTGATGCGCCAACCATCGCTGTTGTAAGGAGTACAACAATGCGACGGTTGAGCATAATGATGCTTTTAGCAAGTTTGC
TCGTTGCTTGTGGTGGTGGA

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDLLKGKKALIVGIANDHSIAWGIAQALKQAGAELAFSYAGESLERRVRPLAEQLGVSFVQQCDVSDDAQIATLFERLGA
EFGSFDILVHSVAYAKREDLSGRFVDSSRDGWRIAMDVSAFSLVALTKAAMPLLNPNASILTLSYYGAEKVIPNYNVMGV
AKAALEATVRYLAADLGPDGLRVNAISAGPIRTLSASGIGNFRRLHRAFTNANPMRRAITIEDVGNSALWLCSELASGVT
GEVVYVDGGYNVLGMSEEEQSS

Sequences:

>Translated_262_residues
MDLLKGKKALIVGIANDHSIAWGIAQALKQAGAELAFSYAGESLERRVRPLAEQLGVSFVQQCDVSDDAQIATLFERLGA
EFGSFDILVHSVAYAKREDLSGRFVDSSRDGWRIAMDVSAFSLVALTKAAMPLLNPNASILTLSYYGAEKVIPNYNVMGV
AKAALEATVRYLAADLGPDGLRVNAISAGPIRTLSASGIGNFRRLHRAFTNANPMRRAITIEDVGNSALWLCSELASGVT
GEVVYVDGGYNVLGMSEEEQSS
>Mature_262_residues
MDLLKGKKALIVGIANDHSIAWGIAQALKQAGAELAFSYAGESLERRVRPLAEQLGVSFVQQCDVSDDAQIATLFERLGA
EFGSFDILVHSVAYAKREDLSGRFVDSSRDGWRIAMDVSAFSLVALTKAAMPLLNPNASILTLSYYGAEKVIPNYNVMGV
AKAALEATVRYLAADLGPDGLRVNAISAGPIRTLSASGIGNFRRLHRAFTNANPMRRAITIEDVGNSALWLCSELASGVT
GEVVYVDGGYNVLGMSEEEQSS

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=28.1746031746032, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4503301, Length=210, Percent_Identity=29.5238095238095, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=27.7992277992278, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7705925, Length=256, Percent_Identity=27.34375, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=260, Percent_Identity=53.8461538461538, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=29.3172690763052, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=28.6821705426357, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=200, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=200, Percent_Identity=25, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=266, Percent_Identity=28.1954887218045, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355319, Length=255, Percent_Identity=29.8039215686275, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI23397609, Length=244, Percent_Identity=26.6393442622951, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28033; Mature: 28033

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLKGKKALIVGIANDHSIAWGIAQALKQAGAELAFSYAGESLERRVRPLAEQLGVSFV
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQCDVSDDAQIATLFERLGAEFGSFDILVHSVAYAKREDLSGRFVDSSRDGWRIAMDVSA
HHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEHHH
FSLVALTKAAMPLLNPNASILTLSYYGAEKVIPNYNVMGVAKAALEATVRYLAADLGPDG
HHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
LRVNAISAGPIRTLSASGIGNFRRLHRAFTNANPMRRAITIEDVGNSALWLCSELASGVT
EEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHCCCC
GEVVYVDGGYNVLGMSEEEQSS
CEEEEEECCCEEEECCCHHCCC
>Mature Secondary Structure
MDLLKGKKALIVGIANDHSIAWGIAQALKQAGAELAFSYAGESLERRVRPLAEQLGVSFV
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQCDVSDDAQIATLFERLGAEFGSFDILVHSVAYAKREDLSGRFVDSSRDGWRIAMDVSA
HHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEHHH
FSLVALTKAAMPLLNPNASILTLSYYGAEKVIPNYNVMGVAKAALEATVRYLAADLGPDG
HHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
LRVNAISAGPIRTLSASGIGNFRRLHRAFTNANPMRRAITIEDVGNSALWLCSELASGVT
EEEEEECCCCCEEEECCCCHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHCCCC
GEVVYVDGGYNVLGMSEEEQSS
CEEEEEECCCEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]