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The map label for this gene is nqo13 [H]

Identifier: 159899730

GI number: 159899730

Start: 4061308

End: 4062861

Strand: Reverse

Name: nqo13 [H]

Synonym: Haur_3212

Alternate gene names: 159899730

Gene position: 4062861-4061308 (Counterclockwise)

Preceding gene: 159899731

Following gene: 159899729

Centisome position: 64.02

GC content: 49.87

Gene sequence:

>1554_bases
TTGACGAATACAAACGACTTAGGTTTTACGATCTTATCGCTGGTGGTGTTTTTACCTGCCGTCGGTGCGATCATCCTGGC
GTTTATGCGCGGCCAACGCGGAGCAGCCCGAATTTTTGCCTTGGGCTGGAGCACACTCGTTTTTGCCGTGTCATTGCCAA
TTTTGTTCAATTTCAAGCACGATGCCGCTGGTCTTCAGTTCTACGAATATTTCAACTGGATTCCAGATTGGGGCGTGCGC
TACGAGTTGGGTCTTGATGGCATTAGTTTGTGGTTGGTCTTACTCACAACCTTTGTCACTCCTTTGGCAATTTGGAGTGC
CTGGAATGTTGAAGAGAAAACCAACAACTTTTTGATGCTAATTCTGTTGCAAGAAATGGGAATGTTGGGCGTGTTCCTCG
CTCAAGACCTGTTCTTGTTCTATATCTTCTGGGAATTTACCCTCATCCCAATGTATTTCTTGATTGGGATGTGGGGTGGC
AAGCGCCGCATTTATGCCACAGTGAAGTTCTTCTTGTATACCTTCGCTGCCTCGGTGTTTATGCTGATCGGCATTATTGC
CTTGGGCTTGCTCAATTACAAATATTTGGCGCAACAAGGCTTGCCAACTGATACTGCCTTCCAAGTGCAAACGTTGATGG
CCAATATCAATCAATTTGGCTTGTCGGATACCGAATTGAAATTGCTGTTCCTCTCGTTCTTCGTGGCGTTTGCGGTCAAA
GTGCCATTGTGGCCTGTCCACACATGGTTGCCCGATGCCCACGTTGAAGCGCCAACCGCTGGCTCAGTGATTCTGGCGGC
AGTGCTGCTGAAAATGGGCGGCTACGGCATGATTCGCTTCTGCGTCCAGTTGTTCCCGCAAATTGCCCAGGAATGGGCAC
GGCCAATTGCCTTGTTGGCAGTAATCGGGATCATTTATGGGGCAGTGCTTTCATATGCTCAAAGCGATATCAAAAAGCTG
GTGGCCTACTCATCAGTCAGCCACATGGGCTTTATCGTCTTGGGGATTTTCGCGCTGAATGAAGTTGGTTTGCAAGGCGC
TGTATTGCAAATGATCAACCACGGGTTGAGCACTGGCGCATTGTTCTTGCTGATTGGGATGGTTTACGAACGCACCCACA
CCCGCGAATTGGCCGATTACGGCGGTTTGTGGAAAGTTGTGCCAGTGTTCGTTTACTTCCTGTTGTTTGCTACGATGGCC
TCGGTCGGCTTGCCTGGCTTGAACGGCTTCGTTGGCGAATTCTTGATTATGCTCGGCGCGTTCGATTCGCCAATCGGCTG
GACGTTCACCGCGTTTGCTGCGGTTGGGGTGATTTTGGCAGCGGTCTATTTGCTCAAGATGTTCCAAGGCATCGCTTCGG
GGCCATTTAGCGAACGTCGCACGCCACACCTCAGCGATTTGACCCGCCGTGAAGTAGCGATTTTGTTGCCACTCTGTATC
TTGATGTTGGTAATTGGTCTCTACTCGTCATACTTTACTCGCCCAATGGAAACAACGATCAAGGCGCTTGAAGCCTTCGT
TGCTAATGCTGCGCCACTGATTGCTGGCCAATAA

Upstream 100 bases:

>100_bases
CTGATGTTTGTGATCGGTGTGGTGATCGTGCTTGGCTTCTTTGCGTTGCGCTAGGACGTGGAGCGCAGGCCTCGGCTTGC
AACTTCACAAGGAGTAACCC

Downstream 100 bases:

>100_bases
GACGGAGCGTGAGTTCGAATGAATGATTTAACATTTATCACGCCGGAGATTAACTGGACAGTCTTAGGCATGCCAGCATT
CCTGATGATTTGGGCGATGT

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTNTNDLGFTILSLVVFLPAVGAIILAFMRGQRGAARIFALGWSTLVFAVSLPILFNFKHDAAGLQFYEYFNWIPDWGVR
YELGLDGISLWLVLLTTFVTPLAIWSAWNVEEKTNNFLMLILLQEMGMLGVFLAQDLFLFYIFWEFTLIPMYFLIGMWGG
KRRIYATVKFFLYTFAASVFMLIGIIALGLLNYKYLAQQGLPTDTAFQVQTLMANINQFGLSDTELKLLFLSFFVAFAVK
VPLWPVHTWLPDAHVEAPTAGSVILAAVLLKMGGYGMIRFCVQLFPQIAQEWARPIALLAVIGIIYGAVLSYAQSDIKKL
VAYSSVSHMGFIVLGIFALNEVGLQGAVLQMINHGLSTGALFLLIGMVYERTHTRELADYGGLWKVVPVFVYFLLFATMA
SVGLPGLNGFVGEFLIMLGAFDSPIGWTFTAFAAVGVILAAVYLLKMFQGIASGPFSERRTPHLSDLTRREVAILLPLCI
LMLVIGLYSSYFTRPMETTIKALEAFVANAAPLIAGQ

Sequences:

>Translated_517_residues
MTNTNDLGFTILSLVVFLPAVGAIILAFMRGQRGAARIFALGWSTLVFAVSLPILFNFKHDAAGLQFYEYFNWIPDWGVR
YELGLDGISLWLVLLTTFVTPLAIWSAWNVEEKTNNFLMLILLQEMGMLGVFLAQDLFLFYIFWEFTLIPMYFLIGMWGG
KRRIYATVKFFLYTFAASVFMLIGIIALGLLNYKYLAQQGLPTDTAFQVQTLMANINQFGLSDTELKLLFLSFFVAFAVK
VPLWPVHTWLPDAHVEAPTAGSVILAAVLLKMGGYGMIRFCVQLFPQIAQEWARPIALLAVIGIIYGAVLSYAQSDIKKL
VAYSSVSHMGFIVLGIFALNEVGLQGAVLQMINHGLSTGALFLLIGMVYERTHTRELADYGGLWKVVPVFVYFLLFATMA
SVGLPGLNGFVGEFLIMLGAFDSPIGWTFTAFAAVGVILAAVYLLKMFQGIASGPFSERRTPHLSDLTRREVAILLPLCI
LMLVIGLYSSYFTRPMETTIKALEAFVANAAPLIAGQ
>Mature_516_residues
TNTNDLGFTILSLVVFLPAVGAIILAFMRGQRGAARIFALGWSTLVFAVSLPILFNFKHDAAGLQFYEYFNWIPDWGVRY
ELGLDGISLWLVLLTTFVTPLAIWSAWNVEEKTNNFLMLILLQEMGMLGVFLAQDLFLFYIFWEFTLIPMYFLIGMWGGK
RRIYATVKFFLYTFAASVFMLIGIIALGLLNYKYLAQQGLPTDTAFQVQTLMANINQFGLSDTELKLLFLSFFVAFAVKV
PLWPVHTWLPDAHVEAPTAGSVILAAVLLKMGGYGMIRFCVQLFPQIAQEWARPIALLAVIGIIYGAVLSYAQSDIKKLV
AYSSVSHMGFIVLGIFALNEVGLQGAVLQMINHGLSTGALFLLIGMVYERTHTRELADYGGLWKVVPVFVYFLLFATMAS
VGLPGLNGFVGEFLIMLGAFDSPIGWTFTAFAAVGVILAAVYLLKMFQGIASGPFSERRTPHLSDLTRREVAILLPLCIL
MLVIGLYSSYFTRPMETTIKALEAFVANAAPLIAGQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=373, Percent_Identity=35.656836461126, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI251831117, Length=400, Percent_Identity=23.5, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788613, Length=466, Percent_Identity=41.6309012875536, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1788827, Length=301, Percent_Identity=27.5747508305648, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788831, Length=293, Percent_Identity=31.3993174061433, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI145693160, Length=346, Percent_Identity=26.5895953757225, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788614, Length=503, Percent_Identity=24.6520874751491, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788829, Length=309, Percent_Identity=26.537216828479, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI2367154, Length=136, Percent_Identity=33.0882352941176, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57306; Mature: 57175

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TNTNDLGFTILSLVVFLPAVGAIILAFMRGQRGAARIFALGWSTLVFAVSLPILFNFKH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCC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HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]