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The map label for this gene is rfbB [C]

Identifier: 159899402

GI number: 159899402

Start: 3655155

End: 3656201

Strand: Reverse

Name: rfbB [C]

Synonym: Haur_2883

Alternate gene names: 159899402

Gene position: 3656201-3655155 (Counterclockwise)

Preceding gene: 159899404

Following gene: 159899401

Centisome position: 57.61

GC content: 47.85

Gene sequence:

>1047_bases
ATGACTCGTCAAAAAGTGCTCGTAACAGGCGGCGCAGGCTTCCTTGGAATTAACCTTTTGCGCTACCTTGATGCCAAAGG
CTACGACTTAGTTTCCTATGATTTTGCCGATTGGACCTATACCGATCTCAAAGATCATATCACGATTGTTAAAGACGATA
TTCGCAACGTTGCTGGGCTTGATCGGGCCATGCAAGGTGTTGATTTCGTTGTGCATACTGCTGCGGCTTTGCCTCTCTAT
CCAGCCGAAGATATTTTTTCGACCGATATTGATGGCACGCGCAATGTGCTGGAAGTTGCCAAAAAACATGGCGTAAAACG
GGTGGTTCACATCTCGTCAACCGCAGTTTATGGCATTCCCGATCACCATCCTTTGGTGGAAAACGACCGCTTGGATGGGG
TTGGGCCATATGGCAAAGCCAAAGTGATGGCCGAATATGTGGCCTTGGAATATCGCGCCAAAGGCATGGAAGTGCCAATT
ATTCGTCCAAAATCGTTTATCGGGCCAGAACGGCTCGGTGTTTTCGCCTTGCTCTACGATTGGGCGATCGACGGCCACAA
CTTCCCAATGATTGGCAATGGCAAAAATCGCTATCAATTGCTTGATGTAGAAGATCTCTGCGATGCGATTTATCTATCGA
TGACCTTGCCTGCTGAAGTTGCTAACGATACTTTCAATATGGGTGCAAAAGAATTCGCCACCATGAAGGAAGATTATCAA
GCAGTACTTGATTACGCTGGCCATGGCAAAAAGATCATCGGTTCGCCAGCAACCCCAGCCATTTGGACGCTGCGAGCCTT
GGAATTTTTGCGGGTTTCGCCGCTCTACAAGTGGGTTTATGAAACGGCCTCGAAAGACTCATTTGTCTCAATCGAAAAAG
CCGAAACCCAACTTGGCTGGAAGCCCAAATTCTCCAACCAAGATGCCTTGATTCGCAACTTCAAGTGGTATATCGACAAT
CGTAACTCATTTGCCAATGCTTCAGGGGTGACCCATCGCGTGCCGTGGAAACAAGGCGCGATTGGTTTGCTCAAAAAAGC
CTTCTAA

Upstream 100 bases:

>100_bases
TGATCGCTGATCCCTAACCCCTAGTGTAGCATAGCCCCAACAAATTGTGATATTATGTGCAAATAATCTAAAATTGAATT
GTTTGAGGATTGATACAATC

Downstream 100 bases:

>100_bases
AAAATCACTACGTCTAGCGTATAATTGCTGGGCGTAGTTTTTTTATTTAATTCGATATGAGGTATCTATGCCCTTAGCAA
TTGCCGTTCACGGTGGCGCT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTRQKVLVTGGAGFLGINLLRYLDAKGYDLVSYDFADWTYTDLKDHITIVKDDIRNVAGLDRAMQGVDFVVHTAAALPLY
PAEDIFSTDIDGTRNVLEVAKKHGVKRVVHISSTAVYGIPDHHPLVENDRLDGVGPYGKAKVMAEYVALEYRAKGMEVPI
IRPKSFIGPERLGVFALLYDWAIDGHNFPMIGNGKNRYQLLDVEDLCDAIYLSMTLPAEVANDTFNMGAKEFATMKEDYQ
AVLDYAGHGKKIIGSPATPAIWTLRALEFLRVSPLYKWVYETASKDSFVSIEKAETQLGWKPKFSNQDALIRNFKWYIDN
RNSFANASGVTHRVPWKQGAIGLLKKAF

Sequences:

>Translated_348_residues
MTRQKVLVTGGAGFLGINLLRYLDAKGYDLVSYDFADWTYTDLKDHITIVKDDIRNVAGLDRAMQGVDFVVHTAAALPLY
PAEDIFSTDIDGTRNVLEVAKKHGVKRVVHISSTAVYGIPDHHPLVENDRLDGVGPYGKAKVMAEYVALEYRAKGMEVPI
IRPKSFIGPERLGVFALLYDWAIDGHNFPMIGNGKNRYQLLDVEDLCDAIYLSMTLPAEVANDTFNMGAKEFATMKEDYQ
AVLDYAGHGKKIIGSPATPAIWTLRALEFLRVSPLYKWVYETASKDSFVSIEKAETQLGWKPKFSNQDALIRNFKWYIDN
RNSFANASGVTHRVPWKQGAIGLLKKAF
>Mature_347_residues
TRQKVLVTGGAGFLGINLLRYLDAKGYDLVSYDFADWTYTDLKDHITIVKDDIRNVAGLDRAMQGVDFVVHTAAALPLYP
AEDIFSTDIDGTRNVLEVAKKHGVKRVVHISSTAVYGIPDHHPLVENDRLDGVGPYGKAKVMAEYVALEYRAKGMEVPII
RPKSFIGPERLGVFALLYDWAIDGHNFPMIGNGKNRYQLLDVEDLCDAIYLSMTLPAEVANDTFNMGAKEFATMKEDYQA
VLDYAGHGKKIIGSPATPAIWTLRALEFLRVSPLYKWVYETASKDSFVSIEKAETQLGWKPKFSNQDALIRNFKWYIDNR
NSFANASGVTHRVPWKQGAIGLLKKAF

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI239745448, Length=346, Percent_Identity=26.5895953757225, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI310132178, Length=346, Percent_Identity=26.3005780346821, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310113012, Length=346, Percent_Identity=26.3005780346821, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=24.5454545454545, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=202, Percent_Identity=29.2079207920792, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38944; Mature: 38813

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQKVLVTGGAGFLGINLLRYLDAKGYDLVSYDFADWTYTDLKDHITIVKDDIRNVAGL
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHCHHHCEEEEHHHHHHHHHH
DRAMQGVDFVVHTAAALPLYPAEDIFSTDIDGTRNVLEVAKKHGVKRVVHISSTAVYGIP
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECEEEECCC
DHHPLVENDRLDGVGPYGKAKVMAEYVALEYRAKGMEVPIIRPKSFIGPERLGVFALLYD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHH
WAIDGHNFPMIGNGKNRYQLLDVEDLCDAIYLSMTLPAEVANDTFNMGAKEFATMKEDYQ
HEECCCCCCEECCCCCCEEEECHHHHHHHHHHHEECCHHHCCCHHCCCHHHHHHHHHHHH
AVLDYAGHGKKIIGSPATPAIWTLRALEFLRVSPLYKWVYETASKDSFVSIEKAETQLGW
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCC
KPKFSNQDALIRNFKWYIDNRNSFANASGVTHRVPWKQGAIGLLKKAF
CCCCCCCCHHHHEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHCC
>Mature Secondary Structure 
TRQKVLVTGGAGFLGINLLRYLDAKGYDLVSYDFADWTYTDLKDHITIVKDDIRNVAGL
CCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHCHHHCEEEEHHHHHHHHHH
DRAMQGVDFVVHTAAALPLYPAEDIFSTDIDGTRNVLEVAKKHGVKRVVHISSTAVYGIP
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECEEEECCC
DHHPLVENDRLDGVGPYGKAKVMAEYVALEYRAKGMEVPIIRPKSFIGPERLGVFALLYD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHH
WAIDGHNFPMIGNGKNRYQLLDVEDLCDAIYLSMTLPAEVANDTFNMGAKEFATMKEDYQ
HEECCCCCCEECCCCCCEEEECHHHHHHHHHHHEECCHHHCCCHHCCCHHHHHHHHHHHH
AVLDYAGHGKKIIGSPATPAIWTLRALEFLRVSPLYKWVYETASKDSFVSIEKAETQLGW
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCC
KPKFSNQDALIRNFKWYIDNRNSFANASGVTHRVPWKQGAIGLLKKAF
CCCCCCCCHHHHEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]