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The map label for this gene is metB [H]

Identifier: 159899326

GI number: 159899326

Start: 3568949

End: 3570070

Strand: Reverse

Name: metB [H]

Synonym: Haur_2807

Alternate gene names: 159899326

Gene position: 3570070-3568949 (Counterclockwise)

Preceding gene: 159899331

Following gene: 159899324

Centisome position: 56.25

GC content: 56.15

Gene sequence:

>1122_bases
ATGGGCCAAGATTCAGCCAGCATCAAACCAGAATCATGGTTGGTTTCAGCGGGGCGCGGTTCGCAGCCAGGCGATCCATT
GAATGTGCCCTTGGTTCCTGCATCGAACTTTATCATTGGCAGTGGCCGTGAATATTCGCGTGATGATGGCACGCCAACTT
GGGAAGCGCTTGAGGCGGTGGTTGGTGGCTTAGAGGGTGGCAAGGCCTTGGCGTTTGCTTCGGGCATGGCTGCGATTGCC
GCAGTGTTTGATCAACTGGCGGTTGGCTCACAGGTTGCCTTGCCCGATGATTGTTATCAAGGCGTTGCTGGCCTTGCTGC
TGCTGGACAACAGCGTGGGCGTTGGTCGGTGCAGCGGGTAGCGGTCGATGATACTGAAGCATGGGTGCGAGCCTGTGCCG
ATGCCGACCTCATTTGGCTTGAATCGCCATCGAATCCATTGCTGACCGTGGCCGATCTTGAACTGATTTGTGCTGCGCCA
CGTAAACCCAGCGCGATTGTCGTGGTCGATAATACCTTTGCCACGCCGTTAAATCAGCAACCACTGGCGTTTGGCGCAAC
CGTTGCAATTCAATCGGCGACGAAATTTATTGGCGGCCACTCAGATTTATTGGCTGGCGTAGCGACGACCAACGATGCGG
CGCTTTGGCATGCGCTCAAGAAATCGCGTGAGTTAACTGGCGCAACCCCTGGCACGCTCGAAGCTTATTTGGCGGTACGC
GGTGCACGCACCTTGGCGGTGCGTTTGCAACGGGCGCAACAAACGGCCATGCTGCTGGCCGAGCGACTTGAGGCTCATCC
ACAGATTATGCGCGTGCGTTACCCTGGGCTTCGATCGCACCCAACCCATGCCGTCGCTCAACGGGTACTCAAGGGTTTTG
GCACAATTATTTCGTTTGATGTGTTGGGTGGCGCTGTGGCTGCTGATCATGTGTGTCAGCAAGTTAAATTAATTCATCAT
GCCACGAGCCTTGGAGCAGTTGAATCGACCATGGAACGACGGGCGGCAATTCCCGGGCAGGAGCATCTACCGCCAGCCTT
GTTGCGTTTGAGCGTTGGGATTGAGGATGCCGCCGATCTGTGGCATGATCTAGAATCGGCTATCGCCGCAGCAACTCGCT
AA

Upstream 100 bases:

>100_bases
ATTAAGCGATTGTCAAGCCTCGCAATTTGAAGTGCTTGCGCTGATTTGCTATGATCGTGCTCGGGCTTCAGGATTTTCGT
TGTTTGCGAGGTACTGCACA

Downstream 100 bases:

>100_bases
ACTGTGATCGCTCGTTCCCAGTCATCAAGCATGAAGGAGGGAACGCCCCTCACCCACCGCAGTGGGAGAGGGGTTGGGGT
GAGGGGACTTAAAATCTATT

Product: Cys/Met metabolism pyridoxal-phosphate-dependent protein

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MGQDSASIKPESWLVSAGRGSQPGDPLNVPLVPASNFIIGSGREYSRDDGTPTWEALEAVVGGLEGGKALAFASGMAAIA
AVFDQLAVGSQVALPDDCYQGVAGLAAAGQQRGRWSVQRVAVDDTEAWVRACADADLIWLESPSNPLLTVADLELICAAP
RKPSAIVVVDNTFATPLNQQPLAFGATVAIQSATKFIGGHSDLLAGVATTNDAALWHALKKSRELTGATPGTLEAYLAVR
GARTLAVRLQRAQQTAMLLAERLEAHPQIMRVRYPGLRSHPTHAVAQRVLKGFGTIISFDVLGGAVAADHVCQQVKLIHH
ATSLGAVESTMERRAAIPGQEHLPPALLRLSVGIEDAADLWHDLESAIAAATR

Sequences:

>Translated_373_residues
MGQDSASIKPESWLVSAGRGSQPGDPLNVPLVPASNFIIGSGREYSRDDGTPTWEALEAVVGGLEGGKALAFASGMAAIA
AVFDQLAVGSQVALPDDCYQGVAGLAAAGQQRGRWSVQRVAVDDTEAWVRACADADLIWLESPSNPLLTVADLELICAAP
RKPSAIVVVDNTFATPLNQQPLAFGATVAIQSATKFIGGHSDLLAGVATTNDAALWHALKKSRELTGATPGTLEAYLAVR
GARTLAVRLQRAQQTAMLLAERLEAHPQIMRVRYPGLRSHPTHAVAQRVLKGFGTIISFDVLGGAVAADHVCQQVKLIHH
ATSLGAVESTMERRAAIPGQEHLPPALLRLSVGIEDAADLWHDLESAIAAATR
>Mature_372_residues
GQDSASIKPESWLVSAGRGSQPGDPLNVPLVPASNFIIGSGREYSRDDGTPTWEALEAVVGGLEGGKALAFASGMAAIAA
VFDQLAVGSQVALPDDCYQGVAGLAAAGQQRGRWSVQRVAVDDTEAWVRACADADLIWLESPSNPLLTVADLELICAAPR
KPSAIVVVDNTFATPLNQQPLAFGATVAIQSATKFIGGHSDLLAGVATTNDAALWHALKKSRELTGATPGTLEAYLAVRG
ARTLAVRLQRAQQTAMLLAERLEAHPQIMRVRYPGLRSHPTHAVAQRVLKGFGTIISFDVLGGAVAADHVCQQVKLIHHA
TSLGAVESTMERRAAIPGQEHLPPALLRLSVGIEDAADLWHDLESAIAAATR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=373, Percent_Identity=34.5844504021448, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI299473759, Length=371, Percent_Identity=32.0754716981132, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI25453487, Length=368, Percent_Identity=30.7065217391304, Blast_Score=141, Evalue=9e-34,
Organism=Escherichia coli, GI1790375, Length=344, Percent_Identity=36.9186046511628, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1789383, Length=402, Percent_Identity=30.0995024875622, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=34.8648648648649, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17560056, Length=381, Percent_Identity=32.5459317585302, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=368, Percent_Identity=32.3369565217391, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319307, Length=352, Percent_Identity=35.7954545454545, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321904, Length=325, Percent_Identity=29.8461538461538, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321254, Length=364, Percent_Identity=30.7692307692308, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=317, Percent_Identity=28.0757097791798, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323333, Length=356, Percent_Identity=26.123595505618, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922554, Length=347, Percent_Identity=34.0057636887608, Blast_Score=194, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 39180; Mature: 39049

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQDSASIKPESWLVSAGRGSQPGDPLNVPLVPASNFIIGSGREYSRDDGTPTWEALEAV
CCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCHHHHHHHH
VGGLEGGKALAFASGMAAIAAVFDQLAVGSQVALPDDCYQGVAGLAAAGQQRGRWSVQRV
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
AVDDTEAWVRACADADLIWLESPSNPLLTVADLELICAAPRKPSAIVVVDNTFATPLNQQ
EECCHHHHHHHHCCCCEEEEECCCCCEEEEECCEEEECCCCCCCEEEEECCCCCCCCCCC
PLAFGATVAIQSATKFIGGHSDLLAGVATTNDAALWHALKKSRELTGATPGTLEAYLAVR
CCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GARTLAVRLQRAQQTAMLLAERLEAHPQIMRVRYPGLRSHPTHAVAQRVLKGFGTIISFD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEHH
VLGGAVAADHVCQQVKLIHHATSLGAVESTMERRAAIPGQEHLPPALLRLSVGIEDAADL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHH
WHDLESAIAAATR
HHHHHHHHHHCCC
>Mature Secondary Structure 
GQDSASIKPESWLVSAGRGSQPGDPLNVPLVPASNFIIGSGREYSRDDGTPTWEALEAV
CCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCHHHHHHHH
VGGLEGGKALAFASGMAAIAAVFDQLAVGSQVALPDDCYQGVAGLAAAGQQRGRWSVQRV
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
AVDDTEAWVRACADADLIWLESPSNPLLTVADLELICAAPRKPSAIVVVDNTFATPLNQQ
EECCHHHHHHHHCCCCEEEEECCCCCEEEEECCEEEECCCCCCCEEEEECCCCCCCCCCC
PLAFGATVAIQSATKFIGGHSDLLAGVATTNDAALWHALKKSRELTGATPGTLEAYLAVR
CCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GARTLAVRLQRAQQTAMLLAERLEAHPQIMRVRYPGLRSHPTHAVAQRVLKGFGTIISFD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEHH
VLGGAVAADHVCQQVKLIHHATSLGAVESTMERRAAIPGQEHLPPALLRLSVGIEDAADL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHH
WHDLESAIAAATR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]