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The map label for this gene is spsK [H]

Identifier: 159899116

GI number: 159899116

Start: 3332179

End: 3333027

Strand: Reverse

Name: spsK [H]

Synonym: Haur_2597

Alternate gene names: 159899116

Gene position: 3333027-3332179 (Counterclockwise)

Preceding gene: 159899117

Following gene: 159899115

Centisome position: 52.52

GC content: 56.07

Gene sequence:

>849_bases
ATGCGTGTGTTGATCCTTGGGGCAAGCGGCCAACTGGGCACTGAGTTAGCCAAGACGTTTGCTGACCATCACTTATTAAT
GCCGAGCCATCAAGAACTGGATTTGAGTCAAGCCACTGCGCGGGCGGCTATTAACCAATTAACCCCCGATTTGGTGCTCT
TGCCAGCGGCTTTTACCAATGTCGATGGCTGTGCGCTTGATCCAGCGCGGGCTTTTCGCGAAAATACGCTTGGCCCAAAA
TATGCTGCGCTGGCCTGCCGCGACCGCGATATTCCCTTGGTCTATGTCAGCACCAACGAGGTATTTAGTGGTAGCAGCCA
GCAAGCCTATAGCGAATACGACCAGCCAGCGCCGATCAATGCCTATGGGCGCTCCAAGTGGGGTGGCGAGCAGGCAGTTT
TGCACCATGCACCCAATGTTTTTATCACGCGGGTGGCTTGGTTGTTTGGTGGGCAGCGCAATTTTGTGCGCACGATCGCC
CGTTTAGGCCGCGAACGTTTGCAAACTGGCGAACCACTGCGCGTTGTGACCGACGAAGTTGGTACGCCAACCCATGCCGC
CGAAGCAGCTTGGGCGATTCGCCAACTGGTCGATAGCAATCTGCCAGGCATTTATCATGTGGTCAATGAGGGCGCTTGCT
CACGTCATGAATTGGCATGTGCGGTCTTGGCAGCGGCGGGTATCGATCTGCCTGTTGAGCCAATTACGTCGGCAGAATTC
AGCCGGCCAAGCAGCCCACCGCCATTTTCAGCATTGAGCAACAATGCCGCTGCTGCCTTGGGCATTCGCTTGCGCCGCTG
GCAAGCAGCTGTAATTGCAGCAGTTGCGGCGATCGACGAGCCAGCTTAA

Upstream 100 bases:

>100_bases
GGCGAATATGCCAACCAAATTACCCCTAGCGCCTAAATAAGCCCGATTAATTGACGAATGCGAGGGCTTGTTCTACCATG
CGCCGCGCAAAGGAGTTTAT

Downstream 100 bases:

>100_bases
ATGAGCCATCACATCGATATTCTGATTCCAAATTATAACGGCGCTAGCTTATTGGCTGCTTGCCTTGAGAGCTTGCGCCA
GCAAACCCGCCGCGATTTTC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRVLILGASGQLGTELAKTFADHHLLMPSHQELDLSQATARAAINQLTPDLVLLPAAFTNVDGCALDPARAFRENTLGPK
YAALACRDRDIPLVYVSTNEVFSGSSQQAYSEYDQPAPINAYGRSKWGGEQAVLHHAPNVFITRVAWLFGGQRNFVRTIA
RLGRERLQTGEPLRVVTDEVGTPTHAAEAAWAIRQLVDSNLPGIYHVVNEGACSRHELACAVLAAAGIDLPVEPITSAEF
SRPSSPPPFSALSNNAAAALGIRLRRWQAAVIAAVAAIDEPA

Sequences:

>Translated_282_residues
MRVLILGASGQLGTELAKTFADHHLLMPSHQELDLSQATARAAINQLTPDLVLLPAAFTNVDGCALDPARAFRENTLGPK
YAALACRDRDIPLVYVSTNEVFSGSSQQAYSEYDQPAPINAYGRSKWGGEQAVLHHAPNVFITRVAWLFGGQRNFVRTIA
RLGRERLQTGEPLRVVTDEVGTPTHAAEAAWAIRQLVDSNLPGIYHVVNEGACSRHELACAVLAAAGIDLPVEPITSAEF
SRPSSPPPFSALSNNAAAALGIRLRRWQAAVIAAVAAIDEPA
>Mature_282_residues
MRVLILGASGQLGTELAKTFADHHLLMPSHQELDLSQATARAAINQLTPDLVLLPAAFTNVDGCALDPARAFRENTLGPK
YAALACRDRDIPLVYVSTNEVFSGSSQQAYSEYDQPAPINAYGRSKWGGEQAVLHHAPNVFITRVAWLFGGQRNFVRTIA
RLGRERLQTGEPLRVVTDEVGTPTHAAEAAWAIRQLVDSNLPGIYHVVNEGACSRHELACAVLAAAGIDLPVEPITSAEF
SRPSSPPPFSALSNNAAAALGIRLRRWQAAVIAAVAAIDEPA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=256, Percent_Identity=26.171875, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI11034825, Length=256, Percent_Identity=26.171875, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788352, Length=290, Percent_Identity=28.6206896551724, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 30231; Mature: 30231

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLILGASGQLGTELAKTFADHHLLMPSHQELDLSQATARAAINQLTPDLVLLPAAFTN
CEEEEEECCCCHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHCC
VDGCALDPARAFRENTLGPKYAALACRDRDIPLVYVSTNEVFSGSSQQAYSEYDQPAPIN
CCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCHHCCCCHHHHHHCCCCCCCC
AYGRSKWGGEQAVLHHAPNVFITRVAWLFGGQRNFVRTIARLGRERLQTGEPLRVVTDEV
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
GTPTHAAEAAWAIRQLVDSNLPGIYHVVNEGACSRHELACAVLAAAGIDLPVEPITSAEF
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
SRPSSPPPFSALSNNAAAALGIRLRRWQAAVIAAVAAIDEPA
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVLILGASGQLGTELAKTFADHHLLMPSHQELDLSQATARAAINQLTPDLVLLPAAFTN
CEEEEEECCCCHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHCC
VDGCALDPARAFRENTLGPKYAALACRDRDIPLVYVSTNEVFSGSSQQAYSEYDQPAPIN
CCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEECCCHHCCCCHHHHHHCCCCCCCC
AYGRSKWGGEQAVLHHAPNVFITRVAWLFGGQRNFVRTIARLGRERLQTGEPLRVVTDEV
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
GTPTHAAEAAWAIRQLVDSNLPGIYHVVNEGACSRHELACAVLAAAGIDLPVEPITSAEF
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
SRPSSPPPFSALSNNAAAALGIRLRRWQAAVIAAVAAIDEPA
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]