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The map label for this gene is nikA [H]

Identifier: 159898918

GI number: 159898918

Start: 3053669

End: 3055462

Strand: Reverse

Name: nikA [H]

Synonym: Haur_2397

Alternate gene names: 159898918

Gene position: 3055462-3053669 (Counterclockwise)

Preceding gene: 159898919

Following gene: 159898917

Centisome position: 48.14

GC content: 49.28

Gene sequence:

>1794_bases
ATGAAGCTACGGTTTCCTGCGTTGATGTTGCTGGTTATTATCTTTACCAGCATGTTGGCCGCATGTGGTGATGCGTCAAC
CCTCACACCACAAGCAACTCAAGCACCAAGTACAACCGCCCCAGCCGCTACTGCAACAGAAGCTCCTGCCACGGGCAGCA
CCGATCCAACCGCCGAACCAACTGCTGCTGCAACCACAGATACCGGTTCCACGAGCAGTGATGGCAAACTATTTTTGACG
GTTTCAGACCAACAACAATCAACTTGGGTGCGCAATTTCAACCCATTTGCTAGTGATAACCGCTGGCCAACTGCCGCCGG
GATTTACGAGCCAATGTTTATTTACAACATTGCCACCGGTAAAATCGAGCCATGGCTCGCCACCGAATGGGCTTGGAATA
GCGATAACACCGAGTTGACCTTCACTATCCGCGATGGCGTGAAGTGGTCAGACGGCGAAGCATTTAGCTCAAAAGACGTT
GCCTACACGCTCAATTTGATGAAAGAAAACGAAAAGCTGCAAGGCAATGGCCGCGCTGCAATGCGTTTTGTTGATAGCGT
CGCTGCCGATGGCAATAAAGTTGTCGTGAAGTTCAGCGAAGTTTCAACCATCGCGCTGTATGATATTGGCCATCAAATGA
TTGTGCCAGAACATATCTGGAGCAAGATCGCTGATCCAGTGACCTTCACCAACGAAACTCCAGTTGCAACCGGTCCATTC
ACCGAAATCATCCGCTTCCAAGACCAAATCTGGGAACTTGGCAAGAATCCAAACTACTGGCAAGCTGGCAAACCATATAT
CGATGGTATTCGCCAACCAGCCTACCCCAGCAACGATGCCGCCAACTTGGCAACGATCAATGGCGAAAACGACTTGGCTA
GCAACTTCATTCCTGATGTTGAAAACACCTATGTCGCCAAAGATCCTGAAAACAACCACTACTGGTTCCCACCAGTCAAT
GCGCCAGTGATGTTGCTCTTGAACACCACCAAGGCTCCATTCAACGATCCTAACGTGCGCAAAGCGATCAGCATGGGCTT
CGATCGCCAACAAATTACTGAAATTGCTACCTACAGCTACAACGGCCCTTCAGATGCAACTGGTTTGCCTGAATCGTTTG
CTGATTGGAAAAACCCTGAAGCCGTTGCTGCTGGCGATTGGGTCAACTTTGATGTTGAAAAAGCCAATGCAATGTTGGAT
GCTGCTGGCCTGACCCGTGGCGCTGATGGGATTCGCGTATTGCCCGATGGCACGCCAATGACCTACGATATCAACGTGGT
TTCTGGCTGGACCGACTGGGTGACCACCGACCAAATCATTGCTGAAAGCTTGAAAGAAATCGGCATCAACGCCACTACCC
GTACCTACGACTTTAGCGCTTGGTTCGATAAAGTTCAAAAGGGCGAGTTTGATATGTCGATTGGCTGGAGCAACAATGCC
CCAACCCCACTCCAATTCTATCGTGGCTTGATGTCAGGTGAAACCGCCGATACCCCAATTGGCGAAGCCAACGGCGACAA
CTGGCACCGCTATGGTAATCCAAAGGTCGATGAATTGTACTCACAATTTGTCAAAACCTCAGACCCAGCCGAACAAAAGA
AAATCATGAACGAAATTCAGATGATCTTTGTTCAAGAAGCCCCAGCTATCCCAATTATGCCAAACATCTATTGGGGTGAA
TACAACACCAAACGCTTTACCAACTTCCCGAACGAAGAAAACCCCTATGTCTTGCTTTCATCGTTCGCCCAACCCGACCG
CTTGATCCTCTTGACCAACATCAAACCAAAATAA

Upstream 100 bases:

>100_bases
GTAACGCGACAAAGTTGTCGCCCGCCTGTGACGTAGGGCCCACTGAGCAGCGCGGGTGTGTGGATAGAACGATCTATCGC
ATACAGAAAGGGTATCCACC

Downstream 100 bases:

>100_bases
GCTGTGTCTGGTTTGGTCGTGTGCTGGAGCCACTAGGCTCCAGCACCAACCCGACCCAGGCGCGAAACCCTGAAAGGAAG
AACCATGCGTTTCTTGTTGC

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MKLRFPALMLLVIIFTSMLAACGDASTLTPQATQAPSTTAPAATATEAPATGSTDPTAEPTAAATTDTGSTSSDGKLFLT
VSDQQQSTWVRNFNPFASDNRWPTAAGIYEPMFIYNIATGKIEPWLATEWAWNSDNTELTFTIRDGVKWSDGEAFSSKDV
AYTLNLMKENEKLQGNGRAAMRFVDSVAADGNKVVVKFSEVSTIALYDIGHQMIVPEHIWSKIADPVTFTNETPVATGPF
TEIIRFQDQIWELGKNPNYWQAGKPYIDGIRQPAYPSNDAANLATINGENDLASNFIPDVENTYVAKDPENNHYWFPPVN
APVMLLLNTTKAPFNDPNVRKAISMGFDRQQITEIATYSYNGPSDATGLPESFADWKNPEAVAAGDWVNFDVEKANAMLD
AAGLTRGADGIRVLPDGTPMTYDINVVSGWTDWVTTDQIIAESLKEIGINATTRTYDFSAWFDKVQKGEFDMSIGWSNNA
PTPLQFYRGLMSGETADTPIGEANGDNWHRYGNPKVDELYSQFVKTSDPAEQKKIMNEIQMIFVQEAPAIPIMPNIYWGE
YNTKRFTNFPNEENPYVLLSSFAQPDRLILLTNIKPK

Sequences:

>Translated_597_residues
MKLRFPALMLLVIIFTSMLAACGDASTLTPQATQAPSTTAPAATATEAPATGSTDPTAEPTAAATTDTGSTSSDGKLFLT
VSDQQQSTWVRNFNPFASDNRWPTAAGIYEPMFIYNIATGKIEPWLATEWAWNSDNTELTFTIRDGVKWSDGEAFSSKDV
AYTLNLMKENEKLQGNGRAAMRFVDSVAADGNKVVVKFSEVSTIALYDIGHQMIVPEHIWSKIADPVTFTNETPVATGPF
TEIIRFQDQIWELGKNPNYWQAGKPYIDGIRQPAYPSNDAANLATINGENDLASNFIPDVENTYVAKDPENNHYWFPPVN
APVMLLLNTTKAPFNDPNVRKAISMGFDRQQITEIATYSYNGPSDATGLPESFADWKNPEAVAAGDWVNFDVEKANAMLD
AAGLTRGADGIRVLPDGTPMTYDINVVSGWTDWVTTDQIIAESLKEIGINATTRTYDFSAWFDKVQKGEFDMSIGWSNNA
PTPLQFYRGLMSGETADTPIGEANGDNWHRYGNPKVDELYSQFVKTSDPAEQKKIMNEIQMIFVQEAPAIPIMPNIYWGE
YNTKRFTNFPNEENPYVLLSSFAQPDRLILLTNIKPK
>Mature_597_residues
MKLRFPALMLLVIIFTSMLAACGDASTLTPQATQAPSTTAPAATATEAPATGSTDPTAEPTAAATTDTGSTSSDGKLFLT
VSDQQQSTWVRNFNPFASDNRWPTAAGIYEPMFIYNIATGKIEPWLATEWAWNSDNTELTFTIRDGVKWSDGEAFSSKDV
AYTLNLMKENEKLQGNGRAAMRFVDSVAADGNKVVVKFSEVSTIALYDIGHQMIVPEHIWSKIADPVTFTNETPVATGPF
TEIIRFQDQIWELGKNPNYWQAGKPYIDGIRQPAYPSNDAANLATINGENDLASNFIPDVENTYVAKDPENNHYWFPPVN
APVMLLLNTTKAPFNDPNVRKAISMGFDRQQITEIATYSYNGPSDATGLPESFADWKNPEAVAAGDWVNFDVEKANAMLD
AAGLTRGADGIRVLPDGTPMTYDINVVSGWTDWVTTDQIIAESLKEIGINATTRTYDFSAWFDKVQKGEFDMSIGWSNNA
PTPLQFYRGLMSGETADTPIGEANGDNWHRYGNPKVDELYSQFVKTSDPAEQKKIMNEIQMIFVQEAPAIPIMPNIYWGE
YNTKRFTNFPNEENPYVLLSSFAQPDRLILLTNIKPK

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=485, Percent_Identity=23.7113402061856, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787052, Length=455, Percent_Identity=25.7142857142857, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789966, Length=489, Percent_Identity=22.4948875255624, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787762, Length=469, Percent_Identity=23.6673773987207, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66027; Mature: 66027

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRFPALMLLVIIFTSMLAACGDASTLTPQATQAPSTTAPAATATEAPATGSTDPTAEP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TAAATTDTGSTSSDGKLFLTVSDQQQSTWVRNFNPFASDNRWPTAAGIYEPMFIYNIATG
CCCEECCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCC
KIEPWLATEWAWNSDNTELTFTIRDGVKWSDGEAFSSKDVAYTLNLMKENEKLQGNGRAA
CCCCCEEEEEEECCCCCEEEEEEECCCEECCCCCCCCCCEEEEEEEHHCCCCCCCCCHHH
MRFVDSVAADGNKVVVKFSEVSTIALYDIGHQMIVPEHIWSKIADPVTFTNETPVATGPF
HHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEECHHHHHHHHCCCEEECCCCCCCCCCH
TEIIRFQDQIWELGKNPNYWQAGKPYIDGIRQPAYPSNDAANLATINGENDLASNFIPDV
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCC
ENTYVAKDPENNHYWFPPVNAPVMLLLNTTKAPFNDPNVRKAISMGFDRQQITEIATYSY
CCCEEECCCCCCCEECCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHEECC
NGPSDATGLPESFADWKNPEAVAAGDWVNFDVEKANAMLDAAGLTRGADGIRVLPDGTPM
CCCCCCCCCCHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHCCCCCCCCCEECCCCCCE
TYDINVVSGWTDWVTTDQIIAESLKEIGINATTRTYDFSAWFDKVQKGEFDMSIGWSNNA
EEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCC
PTPLQFYRGLMSGETADTPIGEANGDNWHRYGNPKVDELYSQFVKTSDPAEQKKIMNEIQ
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
MIFVQEAPAIPIMPNIYWGEYNTKRFTNFPNEENPYVLLSSFAQPDRLILLTNIKPK
EEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKLRFPALMLLVIIFTSMLAACGDASTLTPQATQAPSTTAPAATATEAPATGSTDPTAEP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TAAATTDTGSTSSDGKLFLTVSDQQQSTWVRNFNPFASDNRWPTAAGIYEPMFIYNIATG
CCCEECCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCC
KIEPWLATEWAWNSDNTELTFTIRDGVKWSDGEAFSSKDVAYTLNLMKENEKLQGNGRAA
CCCCCEEEEEEECCCCCEEEEEEECCCEECCCCCCCCCCEEEEEEEHHCCCCCCCCCHHH
MRFVDSVAADGNKVVVKFSEVSTIALYDIGHQMIVPEHIWSKIADPVTFTNETPVATGPF
HHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEECHHHHHHHHCCCEEECCCCCCCCCCH
TEIIRFQDQIWELGKNPNYWQAGKPYIDGIRQPAYPSNDAANLATINGENDLASNFIPDV
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCC
ENTYVAKDPENNHYWFPPVNAPVMLLLNTTKAPFNDPNVRKAISMGFDRQQITEIATYSY
CCCEEECCCCCCCEECCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHEECC
NGPSDATGLPESFADWKNPEAVAAGDWVNFDVEKANAMLDAAGLTRGADGIRVLPDGTPM
CCCCCCCCCCHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHCCCCCCCCCEECCCCCCE
TYDINVVSGWTDWVTTDQIIAESLKEIGINATTRTYDFSAWFDKVQKGEFDMSIGWSNNA
EEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCC
PTPLQFYRGLMSGETADTPIGEANGDNWHRYGNPKVDELYSQFVKTSDPAEQKKIMNEIQ
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
MIFVQEAPAIPIMPNIYWGEYNTKRFTNFPNEENPYVLLSSFAQPDRLILLTNIKPK
EEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]