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The map label for this gene is purR [H]

Identifier: 159898919

GI number: 159898919

Start: 3055559

End: 3056665

Strand: Reverse

Name: purR [H]

Synonym: Haur_2398

Alternate gene names: 159898919

Gene position: 3056665-3055559 (Counterclockwise)

Preceding gene: 159898922

Following gene: 159898918

Centisome position: 48.16

GC content: 51.67

Gene sequence:

>1107_bases
ATGCAAAGGCGCAACAACGCAATGAGCAAAAAACTCGAACAACCAACCCAAACCCTAAAAACGCCCCGAGGCCGCCGCCC
GAATGGCAATGGCCTTAGCAGCACCACGATTATGGATGTGGCGCGTGAAGCTGGTGTCTCGTATGCAACTGTCTCGCGAG
TGGTCAATAACAAAGAATATGTTAAATCGGATACTCGTGAACGGGTGCTGAAAGCGATTACAAGCTTGGGCTATGTCGTC
AACCAGCAGGCCAGAAGTTTGGCTGGTGGGCGCTCATATGCAGTTGGGTTGTTGGTGCGCGATCTTGGCTCAAGCTATAT
GGGTGAAATTGTGCGCGGGATTGACGAGTCGCTCAGTGCTGCTCAATACAATCTGATGCTCTATACCACCCATCGCCGCA
AAATCAAAGAACGGATTTACGTCAACAATTTGATCCAGGGCATGACTGATGGGCTGCTCTTGGTTTTGCCAGAAAACCTT
GAAGCCTATTTGGAAACTCTTGATCAGACCAACTTCCCCTATGTCCTGATCGATCATCAAGGGCTTGACGAGCGCACTCC
AGTGGTGATTACCACTAATTGGCAGGGTGGCTACGATGCCACGCGCTATCTGATTGAGCTTGGTCATCGACGGATTGGGT
TTCTGACGGGCATGATGGATATGCGCAGCTCGCAAGATCGCCTGAGTGGTTATCAAGCAGCGCTGCGTGACCATGGTTTG
CCAGCCGATCCACAACTCGTGTATGAAGGAACCTATTATCAACCTGAAGGCTACGCTGGGGCACAAAGCCTGTTGCGGCT
GCCAGAGCCACCAACAGCAATTTTTGCCTCGAACGATGTAATGGCCTTTGGGGTGATGGAAGCGGTTCGCGATGCCGGAT
TGCGGATTCCAAGTGACATCTCGGTGATTGGCTTTGATGATATTGCGCAAGCCTCGCAGGTAGCACCGCCATTGACGACG
GTCGCCCAGCCATTAGAACAACTGGGACGCGAAGCTGTTCGAATGTTGCTAGCTCGGATCAATAATCCTGAGCAACCGAT
AGCGCGTACCATTTTGCCAACTACCTTGATTATTCGCCAATCGTGCGACGTTCCTCGGAACGTGTAA

Upstream 100 bases:

>100_bases
CTGTAAGCGTTTGCAAGAATAAAAAACCGAACAAGCCTCTCTAGGTGCAAAACCCCACAATCGTTCTGTAAATGCTTACA
ATTCACAATCCAGTTCGCAG

Downstream 100 bases:

>100_bases
CGCGACAAAGTTGTCGCCCGCCTGTGACGTAGGGCCCACTGAGCAGCGCGGGTGTGTGGATAGAACGATCTATCGCATAC
AGAAAGGGTATCCACCATGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MQRRNNAMSKKLEQPTQTLKTPRGRRPNGNGLSSTTIMDVAREAGVSYATVSRVVNNKEYVKSDTRERVLKAITSLGYVV
NQQARSLAGGRSYAVGLLVRDLGSSYMGEIVRGIDESLSAAQYNLMLYTTHRRKIKERIYVNNLIQGMTDGLLLVLPENL
EAYLETLDQTNFPYVLIDHQGLDERTPVVITTNWQGGYDATRYLIELGHRRIGFLTGMMDMRSSQDRLSGYQAALRDHGL
PADPQLVYEGTYYQPEGYAGAQSLLRLPEPPTAIFASNDVMAFGVMEAVRDAGLRIPSDISVIGFDDIAQASQVAPPLTT
VAQPLEQLGREAVRMLLARINNPEQPIARTILPTTLIIRQSCDVPRNV

Sequences:

>Translated_368_residues
MQRRNNAMSKKLEQPTQTLKTPRGRRPNGNGLSSTTIMDVAREAGVSYATVSRVVNNKEYVKSDTRERVLKAITSLGYVV
NQQARSLAGGRSYAVGLLVRDLGSSYMGEIVRGIDESLSAAQYNLMLYTTHRRKIKERIYVNNLIQGMTDGLLLVLPENL
EAYLETLDQTNFPYVLIDHQGLDERTPVVITTNWQGGYDATRYLIELGHRRIGFLTGMMDMRSSQDRLSGYQAALRDHGL
PADPQLVYEGTYYQPEGYAGAQSLLRLPEPPTAIFASNDVMAFGVMEAVRDAGLRIPSDISVIGFDDIAQASQVAPPLTT
VAQPLEQLGREAVRMLLARINNPEQPIARTILPTTLIIRQSCDVPRNV
>Mature_368_residues
MQRRNNAMSKKLEQPTQTLKTPRGRRPNGNGLSSTTIMDVAREAGVSYATVSRVVNNKEYVKSDTRERVLKAITSLGYVV
NQQARSLAGGRSYAVGLLVRDLGSSYMGEIVRGIDESLSAAQYNLMLYTTHRRKIKERIYVNNLIQGMTDGLLLVLPENL
EAYLETLDQTNFPYVLIDHQGLDERTPVVITTNWQGGYDATRYLIELGHRRIGFLTGMMDMRSSQDRLSGYQAALRDHGL
PADPQLVYEGTYYQPEGYAGAQSLLRLPEPPTAIFASNDVMAFGVMEAVRDAGLRIPSDISVIGFDDIAQASQVAPPLTT
VAQPLEQLGREAVRMLLARINNPEQPIARTILPTTLIIRQSCDVPRNV

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=35.4354354354354, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=35.0299401197605, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=36.2459546925566, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=33.5311572700297, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=31.437125748503, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=34.0298507462687, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=31.044776119403, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=26.6457680250784, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787906, Length=334, Percent_Identity=29.6407185628743, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.8888888888889, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=26.1006289308176, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786268, Length=314, Percent_Identity=22.9299363057325, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789456, Length=359, Percent_Identity=26.7409470752089, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=26.3473053892216, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790192, Length=282, Percent_Identity=24.822695035461, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40858; Mature: 40858

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRNNAMSKKLEQPTQTLKTPRGRRPNGNGLSSTTIMDVAREAGVSYATVSRVVNNKEY
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHH
VKSDTRERVLKAITSLGYVVNQQARSLAGGRSYAVGLLVRDLGSSYMGEIVRGIDESLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AQYNLMLYTTHRRKIKERIYVNNLIQGMTDGLLLVLPENLEAYLETLDQTNFPYVLIDHQ
HHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCEEEEECC
GLDERTPVVITTNWQGGYDATRYLIELGHRRIGFLTGMMDMRSSQDRLSGYQAALRDHGL
CCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
PADPQLVYEGTYYQPEGYAGAQSLLRLPEPPTAIFASNDVMAFGVMEAVRDAGLRIPSDI
CCCCCEEEECCEECCCCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCC
SVIGFDDIAQASQVAPPLTTVAQPLEQLGREAVRMLLARINNPEQPIARTILPTTLIIRQ
EEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SCDVPRNV
CCCCCCCC
>Mature Secondary Structure
MQRRNNAMSKKLEQPTQTLKTPRGRRPNGNGLSSTTIMDVAREAGVSYATVSRVVNNKEY
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHH
VKSDTRERVLKAITSLGYVVNQQARSLAGGRSYAVGLLVRDLGSSYMGEIVRGIDESLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AQYNLMLYTTHRRKIKERIYVNNLIQGMTDGLLLVLPENLEAYLETLDQTNFPYVLIDHQ
HHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCEEEEECC
GLDERTPVVITTNWQGGYDATRYLIELGHRRIGFLTGMMDMRSSQDRLSGYQAALRDHGL
CCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
PADPQLVYEGTYYQPEGYAGAQSLLRLPEPPTAIFASNDVMAFGVMEAVRDAGLRIPSDI
CCCCCEEEECCEECCCCCCCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCC
SVIGFDDIAQASQVAPPLTTVAQPLEQLGREAVRMLLARINNPEQPIARTILPTTLIIRQ
EEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SCDVPRNV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA