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The map label for this gene is yfiK [H]

Identifier: 159898692

GI number: 159898692

Start: 2752125

End: 2752781

Strand: Direct

Name: yfiK [H]

Synonym: Haur_2171

Alternate gene names: 159898692

Gene position: 2752125-2752781 (Clockwise)

Preceding gene: 159898691

Following gene: 159898700

Centisome position: 43.36

GC content: 52.82

Gene sequence:

>657_bases
ATGATCAAACTATTAATTTGCGATGATCAGGCGGTTGTGCGCGATGGCTTGTCGATCATGTTGAATCTCGCGCCTGATCT
TGAGGTGATTGCCGTCGCCAGCAATGGCCGCGAGGCGGTGCAGCTTAGCGCAACGTGGCAGCCAGATCTGATTTTGATGG
ATCTTAAAATGCCGATTATGAATGGAATCGAGGCCACGCGCCAAATTAGCGCAGCCCAACCACTGGTTAAAATCTTAGTA
TTAACGACCTACGACGATGATGAATGGTTGTTTGAGGCAATTCGGGCGGGAGCAGCAGGCTATTTGCTCAAGGATATGGC
GCATAGCGATTTGGTTAGTGCGATTCGCGGCACAGTTTCGGGCAAAGCCTATGTTGATCCCAATGTGACGGCCCGCCTGC
TCAGTCAAGTTGCCACCTCGCGCAATCAACCACCAGCCAGCCAATTAACTGGCCGCTTGACCGAGCGTGAGCTGGCAGTG
CTCAATTTAATTGCCACAGGCCACACCAACGCCAGTATCGCCGAACAACTCCGACTTTCTGAGGGCACGGTGCGTAACCA
CGTCAGCACCATTTTATCCAAGCTCGATGTCGCTGACCGAACGCAGGCCGCTCTGTTGGCAGTGGAACATGGCTTGCATC
AGCGCGACCATTATTAA

Upstream 100 bases:

>100_bases
GCATGAGCGAGCTGCTGAGTTGGGCGGCCAGCTGAGCATCAACAGCCAGCTTGGGCTGGGCACTTGGGTCGAATTACGCT
TTACAACAAAGGGTTGAGCA

Downstream 100 bases:

>100_bases
AAATCCCTACAATGCTCCTTATTCAACAGCCCGCCCCACGATCCATTATCCAAACGCTAACTCCAACTCATATTTGATAT
ACAAATGTGGGTAAATCTGG

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MIKLLICDDQAVVRDGLSIMLNLAPDLEVIAVASNGREAVQLSATWQPDLILMDLKMPIMNGIEATRQISAAQPLVKILV
LTTYDDDEWLFEAIRAGAAGYLLKDMAHSDLVSAIRGTVSGKAYVDPNVTARLLSQVATSRNQPPASQLTGRLTERELAV
LNLIATGHTNASIAEQLRLSEGTVRNHVSTILSKLDVADRTQAALLAVEHGLHQRDHY

Sequences:

>Translated_218_residues
MIKLLICDDQAVVRDGLSIMLNLAPDLEVIAVASNGREAVQLSATWQPDLILMDLKMPIMNGIEATRQISAAQPLVKILV
LTTYDDDEWLFEAIRAGAAGYLLKDMAHSDLVSAIRGTVSGKAYVDPNVTARLLSQVATSRNQPPASQLTGRLTERELAV
LNLIATGHTNASIAEQLRLSEGTVRNHVSTILSKLDVADRTQAALLAVEHGLHQRDHY
>Mature_218_residues
MIKLLICDDQAVVRDGLSIMLNLAPDLEVIAVASNGREAVQLSATWQPDLILMDLKMPIMNGIEATRQISAAQPLVKILV
LTTYDDDEWLFEAIRAGAAGYLLKDMAHSDLVSAIRGTVSGKAYVDPNVTARLLSQVATSRNQPPASQLTGRLTERELAV
LNLIATGHTNASIAEQLRLSEGTVRNHVSTILSKLDVADRTQAALLAVEHGLHQRDHY

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=213, Percent_Identity=36.150234741784, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=34.9282296650718, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787473, Length=202, Percent_Identity=34.6534653465347, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790102, Length=204, Percent_Identity=29.9019607843137, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786747, Length=192, Percent_Identity=28.125, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788712, Length=172, Percent_Identity=26.1627906976744, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788550, Length=102, Percent_Identity=37.2549019607843, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1790437, Length=122, Percent_Identity=31.1475409836066, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23682; Mature: 23682

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLLICDDQAVVRDGLSIMLNLAPDLEVIAVASNGREAVQLSATWQPDLILMDLKMPIM
CEEEEEECCHHHHHCCHHEEEECCCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCHHH
NGIEATRQISAAQPLVKILVLTTYDDDEWLFEAIRAGAAGYLLKDMAHSDLVSAIRGTVS
HHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
GKAYVDPNVTARLLSQVATSRNQPPASQLTGRLTERELAVLNLIATGHTNASIAEQLRLS
CCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHC
EGTVRNHVSTILSKLDVADRTQAALLAVEHGLHQRDHY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIKLLICDDQAVVRDGLSIMLNLAPDLEVIAVASNGREAVQLSATWQPDLILMDLKMPIM
CEEEEEECCHHHHHCCHHEEEECCCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCHHH
NGIEATRQISAAQPLVKILVLTTYDDDEWLFEAIRAGAAGYLLKDMAHSDLVSAIRGTVS
HHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
GKAYVDPNVTARLLSQVATSRNQPPASQLTGRLTERELAVLNLIATGHTNASIAEQLRLS
CCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHC
EGTVRNHVSTILSKLDVADRTQAALLAVEHGLHQRDHY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]