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The map label for this gene is yfiJ [H]

Identifier: 159898691

GI number: 159898691

Start: 2750805

End: 2752121

Strand: Direct

Name: yfiJ [H]

Synonym: Haur_2170

Alternate gene names: 159898691

Gene position: 2750805-2752121 (Clockwise)

Preceding gene: 159898682

Following gene: 159898692

Centisome position: 43.34

GC content: 51.48

Gene sequence:

>1317_bases
ATGTTATTCCAAGATTCAGCTAAGCTTCGACAATCAGCAGAAACTCAAGCAATACCCATCCCTAACCACACCCATGAAGC
TGAGCAATTCAGCGGTGGCCTATCGATCAAACGCATCCTACAAATCGTGGCCTTGCTCTGGATTAGCTATTTTTTAATTT
TAACGGGCCTTGATTGGGTTTCGTTGATGTCGCGACCTGATACCAGCCCCTTGCCATGGTTCTACTACGCGATTCATATC
ACCTTGGCGGGAGTTGTGTTTGGTTTAGCAACTTGGGATGCGGCTCAACAGCGTTTGGGGCGGGTATTTATGCCCTTGAT
CATTGGGCTGATTTCGGTTGTGCCCATTCTGCTGACCCCATTAATTACCAGCGGGCCAGGCCCAATTACCACTGAGCCAG
GTTTAGTCGTGATGCGTTTGTGCCCGATGCTGTGTGTGGCAGTGGTGATTGTGGCGTGGCAATATACTTGGCGACAGGTG
GTTTGGTTTTGTTTGGTAACTGGGGCATTAATTTTGCTGCCAATTTTGCTCCGTGCCCCACGTTTTAGTTCAGCGGTTGC
GATCACAATTGTCCAAACCAGCACGTTTTTGGTGTTTGGCTATGCGCTATCAGCCTTAGTGCAACGTCTGCGGCTGCAAA
ATGCGGCCTTGATTCAAGCCCATGAGCAGTTGCGTGATTATGCTGGTACTCAAGAGCGGTTGGCAATTAGCCGTGAGCGC
AACCGCGTAGCTCGCGAATTGCACGACACCCTGGCCCATGCCCTGAGCGGCTTGATCGTGCAACTTGAGGCTGCCAAACT
CTACCGTGAAATTGAGCCGACCACCAGCCATAAGCTGCAAGATTCGGCGCTCGATGCCGCCCGCCATGGCTTGCAAGAAA
CCCGTTTAGCGCTCAAAGCCCTCCGTGCCCGCCCGTTGGATGATTTAGGCCTAGCCCTAGCATTACGTCAACTTGCTGAG
CAACTGGCAAGTACCGCGCAGCTTACGCTTGATCTATCGATTTCGCCGAAACTGCCACCCCTAGCCCTAGAAGTTGAAAC
CTGTGTTTATCGGGTTGCCCAAGAAGCACTGCGCAACGCGATTCGCCATGCCAATGCCAACCGTTTAGATGTCAGCTTAA
CCTGTATTGATACCGAATTATGTCTAGTTGTCCGTGATGATGGCTGTGGCTTTGAATTCCAATCATTCTCTACAGCAGAC
CATTTTGGCTTAGCAGGAATGCATGAGCGAGCTGCTGAGTTGGGCGGCCAGCTGAGCATCAACAGCCAGCTTGGGCTGGG
CACTTGGGTCGAATTACGCTTTACAACAAAGGGTTGA

Upstream 100 bases:

>100_bases
GGAGAGGGGTTGGGGGTGAGGGAACGGTTGCCATACCAAATCACTTGAAATCAATTCGTGACGAGAAGTGGATAACCCAC
CAGCACTGCAAGGAACGCCT

Downstream 100 bases:

>100_bases
GCAATGATCAAACTATTAATTTGCGATGATCAGGCGGTTGTGCGCGATGGCTTGTCGATCATGTTGAATCTCGCGCCTGA
TCTTGAGGTGATTGCCGTCG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLFQDSAKLRQSAETQAIPIPNHTHEAEQFSGGLSIKRILQIVALLWISYFLILTGLDWVSLMSRPDTSPLPWFYYAIHI
TLAGVVFGLATWDAAQQRLGRVFMPLIIGLISVVPILLTPLITSGPGPITTEPGLVVMRLCPMLCVAVVIVAWQYTWRQV
VWFCLVTGALILLPILLRAPRFSSAVAITIVQTSTFLVFGYALSALVQRLRLQNAALIQAHEQLRDYAGTQERLAISRER
NRVARELHDTLAHALSGLIVQLEAAKLYREIEPTTSHKLQDSALDAARHGLQETRLALKALRARPLDDLGLALALRQLAE
QLASTAQLTLDLSISPKLPPLALEVETCVYRVAQEALRNAIRHANANRLDVSLTCIDTELCLVVRDDGCGFEFQSFSTAD
HFGLAGMHERAAELGGQLSINSQLGLGTWVELRFTTKG

Sequences:

>Translated_438_residues
MLFQDSAKLRQSAETQAIPIPNHTHEAEQFSGGLSIKRILQIVALLWISYFLILTGLDWVSLMSRPDTSPLPWFYYAIHI
TLAGVVFGLATWDAAQQRLGRVFMPLIIGLISVVPILLTPLITSGPGPITTEPGLVVMRLCPMLCVAVVIVAWQYTWRQV
VWFCLVTGALILLPILLRAPRFSSAVAITIVQTSTFLVFGYALSALVQRLRLQNAALIQAHEQLRDYAGTQERLAISRER
NRVARELHDTLAHALSGLIVQLEAAKLYREIEPTTSHKLQDSALDAARHGLQETRLALKALRARPLDDLGLALALRQLAE
QLASTAQLTLDLSISPKLPPLALEVETCVYRVAQEALRNAIRHANANRLDVSLTCIDTELCLVVRDDGCGFEFQSFSTAD
HFGLAGMHERAAELGGQLSINSQLGLGTWVELRFTTKG
>Mature_438_residues
MLFQDSAKLRQSAETQAIPIPNHTHEAEQFSGGLSIKRILQIVALLWISYFLILTGLDWVSLMSRPDTSPLPWFYYAIHI
TLAGVVFGLATWDAAQQRLGRVFMPLIIGLISVVPILLTPLITSGPGPITTEPGLVVMRLCPMLCVAVVIVAWQYTWRQV
VWFCLVTGALILLPILLRAPRFSSAVAITIVQTSTFLVFGYALSALVQRLRLQNAALIQAHEQLRDYAGTQERLAISRER
NRVARELHDTLAHALSGLIVQLEAAKLYREIEPTTSHKLQDSALDAARHGLQETRLALKALRARPLDDLGLALALRQLAE
QLASTAQLTLDLSISPKLPPLALEVETCVYRVAQEALRNAIRHANANRLDVSLTCIDTELCLVVRDDGCGFEFQSFSTAD
HFGLAGMHERAAELGGQLSINSQLGLGTWVELRFTTKG

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=210, Percent_Identity=33.8095238095238, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787474, Length=206, Percent_Identity=27.6699029126214, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082311, Length=300, Percent_Identity=29, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48318; Mature: 48318

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCEEEEEEEECCC
>Mature Secondary Structure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CCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]