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The map label for this gene is tycC [H]

Identifier: 159898554

GI number: 159898554

Start: 2525082

End: 2526734

Strand: Reverse

Name: tycC [H]

Synonym: Haur_2030

Alternate gene names: 159898554

Gene position: 2526734-2525082 (Counterclockwise)

Preceding gene: 159898555

Following gene: 159898553

Centisome position: 39.81

GC content: 49.73

Gene sequence:

>1653_bases
ATGATGATTGAGTTTAACCAGATGCTTGAAGTTGTGCCTCCATGCCAAAGCGAGCCAAACCAAGCACACGCAACCAGCCA
ATTGCACAGCGGTTTTCTACAGAGCGTCCAGCGCTATCCAAATAACATCGCTTTAACGATTGGTAATAAACAGTATGATT
ATGTCAAATTATATACAGTTGCCCAACGCTGGGCTTTCGCGCTTCGCCAATCAACCAAACCGCTGCATCGTGTTGGTATT
TTTGCCTATCGTAGTGAAGCAGCCTACATCGGGATTTTGGCAAGCTTATTGGCTGGCGCAACCTTTGTGCCGCTGAACTA
CAACTTTCCACTTCAACGGACCCAAGCGATGATCGAGCAAGCAGAGCTTGATGCGATTATTGTCGATCACCAATCGTATG
ACCAATTTTTGCAATTGGCCGATTCGCTGCCAGTACTACCGCCATGTGTCCTCTTGCCTGATTGTTTGCGTGCGCCGCTG
CTTGATACAATGATCTATACTCAAGCCGAGCTTGCTGAGCTACCGACTGATCATGAACCAGTTACTGTGCCGCCTGAGGC
AATTGCCTATCTGTTATTCACTTCGGGTAGCACCGGCAACCCCAAAGGCGTACCAATTAGTCATGCCAATGTCGCACACT
TTCTCAAGGTAAATCAAGCACGATATCAGATTACGCCTGCTGATCGGCTGAGCCAGACCTTTGATCAAACCTTTGATCTG
GCCATCTTTGATCTTTTTATGGCTTGGAATCATGGTGCGGCGGTCTGTGTTATCCAACCGATCCAATTGCTCTCACCTTT
TCGCTTAATTGAAGAGCAGGGAATTACGATTTGGTTTTCGGTACCATCAGTTGCCGCGTTACTGCGCAAACAAAAACTAC
TCAAGCCCAATAGCTTGCCCAACTTACGCTTAAGCCTTTTTTGTGGCGAAGCGTTGCCCAAAGCTACCGCTGAGGCTTGG
CAACTTGCTGCGCCCAACTCAATAATCGACAATCTCTATGGTCCAACCGAATTAACAATCGCCTGTGCAGTGTATCGCTG
GAATTCCCTCACCTCGCCTGCTGAATGTTTGAATGAAGTGGTCCCAATTGGTAAACTCTACCCAGGTTTAACCGCGGTGG
TGGTTGACGCAAACGATAATCCTGTACCAGCAGGTACAGAAGGCGAATTGTGTGTTGCTGGCCCACAAACCTTCCAAGGC
TATTGGCACAACCCAAGCCTCACGGAGCAACGGTTTCTGCGCAGCAAACAGCTTAATGGCGAGGAACTCGGCTACTACCG
CACCGGTGATCGGGTTGTATGCCGCACCAATGGCAGCATGATTTACCTTGGGCGCAGCGATCAACAAATTAAAGTCCATG
GCTACCGGGTGGAATTAAGCGAGATTGAAGGGGCGTTATTACTCCAACCAGGCGTAGTTGCTGCGGTTGCACTGGGCTGG
CCGCTTGAAAACGGTTCGGCGAGCGGAATTGTCGCGTTTGTAATTGCGCCAAGCATTGCAGTCAGTGATCTGCAACAGGC
GGTTCAGCCATTGCTCCCAAGCTATATGCTGCCGCGCACCATCTATCAGCTTGAAACCATGCCGCTGAATGCCAATGGCA
AAATTGATCGGTTGGCCTTGGCTCGCCACTTAGCAGGTGAAGGAACGGCCTAA

Upstream 100 bases:

>100_bases
CATTGCTTGCCTATGTTGGGCCAATTGATCAGCCCTATCCAGCATTGAGCCAACGTCAATAACAAGTCTATAACACGCCT
AACGCCTTATTGGAGAATAG

Downstream 100 bases:

>100_bases
TGCAAACAGTGAATCCAGCATTCCAACGTTCATTGGAGTGTGCAGGCCAAACCTATCGCGTCTGGTCAATTCAAGCACTC
GCAAGTAGCTATCAGCTTGA

Product: amino acid adenylation domain-containing protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: Tyrocidine synthase III; ATP-dependent asparagine adenylase; AsnA; Asparagine activase; ATP-dependent glutamine adenylase; GlnA; Glutamine activase; ATP-dependent tyrosine adenylase; TyrA; Tyrosine activase; ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent ornithine adenylase; OrnA; Ornithine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MMIEFNQMLEVVPPCQSEPNQAHATSQLHSGFLQSVQRYPNNIALTIGNKQYDYVKLYTVAQRWAFALRQSTKPLHRVGI
FAYRSEAAYIGILASLLAGATFVPLNYNFPLQRTQAMIEQAELDAIIVDHQSYDQFLQLADSLPVLPPCVLLPDCLRAPL
LDTMIYTQAELAELPTDHEPVTVPPEAIAYLLFTSGSTGNPKGVPISHANVAHFLKVNQARYQITPADRLSQTFDQTFDL
AIFDLFMAWNHGAAVCVIQPIQLLSPFRLIEEQGITIWFSVPSVAALLRKQKLLKPNSLPNLRLSLFCGEALPKATAEAW
QLAAPNSIIDNLYGPTELTIACAVYRWNSLTSPAECLNEVVPIGKLYPGLTAVVVDANDNPVPAGTEGELCVAGPQTFQG
YWHNPSLTEQRFLRSKQLNGEELGYYRTGDRVVCRTNGSMIYLGRSDQQIKVHGYRVELSEIEGALLLQPGVVAAVALGW
PLENGSASGIVAFVIAPSIAVSDLQQAVQPLLPSYMLPRTIYQLETMPLNANGKIDRLALARHLAGEGTA

Sequences:

>Translated_550_residues
MMIEFNQMLEVVPPCQSEPNQAHATSQLHSGFLQSVQRYPNNIALTIGNKQYDYVKLYTVAQRWAFALRQSTKPLHRVGI
FAYRSEAAYIGILASLLAGATFVPLNYNFPLQRTQAMIEQAELDAIIVDHQSYDQFLQLADSLPVLPPCVLLPDCLRAPL
LDTMIYTQAELAELPTDHEPVTVPPEAIAYLLFTSGSTGNPKGVPISHANVAHFLKVNQARYQITPADRLSQTFDQTFDL
AIFDLFMAWNHGAAVCVIQPIQLLSPFRLIEEQGITIWFSVPSVAALLRKQKLLKPNSLPNLRLSLFCGEALPKATAEAW
QLAAPNSIIDNLYGPTELTIACAVYRWNSLTSPAECLNEVVPIGKLYPGLTAVVVDANDNPVPAGTEGELCVAGPQTFQG
YWHNPSLTEQRFLRSKQLNGEELGYYRTGDRVVCRTNGSMIYLGRSDQQIKVHGYRVELSEIEGALLLQPGVVAAVALGW
PLENGSASGIVAFVIAPSIAVSDLQQAVQPLLPSYMLPRTIYQLETMPLNANGKIDRLALARHLAGEGTA
>Mature_550_residues
MMIEFNQMLEVVPPCQSEPNQAHATSQLHSGFLQSVQRYPNNIALTIGNKQYDYVKLYTVAQRWAFALRQSTKPLHRVGI
FAYRSEAAYIGILASLLAGATFVPLNYNFPLQRTQAMIEQAELDAIIVDHQSYDQFLQLADSLPVLPPCVLLPDCLRAPL
LDTMIYTQAELAELPTDHEPVTVPPEAIAYLLFTSGSTGNPKGVPISHANVAHFLKVNQARYQITPADRLSQTFDQTFDL
AIFDLFMAWNHGAAVCVIQPIQLLSPFRLIEEQGITIWFSVPSVAALLRKQKLLKPNSLPNLRLSLFCGEALPKATAEAW
QLAAPNSIIDNLYGPTELTIACAVYRWNSLTSPAECLNEVVPIGKLYPGLTAVVVDANDNPVPAGTEGELCVAGPQTFQG
YWHNPSLTEQRFLRSKQLNGEELGYYRTGDRVVCRTNGSMIYLGRSDQQIKVHGYRVELSEIEGALLLQPGVVAAVALGW
PLENGSASGIVAFVIAPSIAVSDLQQAVQPLLPSYMLPRTIYQLETMPLNANGKIDRLALARHLAGEGTA

Specific function: Incorporates six amino acids (for tyrocidine A, Asn, Gln, Tyr, Val, Orn, and Leu) in their L-configuration into the peptide product [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=606, Percent_Identity=24.2574257425743, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI187761343, Length=606, Percent_Identity=24.2574257425743, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI157311624, Length=488, Percent_Identity=25.2049180327869, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157311622, Length=488, Percent_Identity=25.2049180327869, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI58082049, Length=488, Percent_Identity=24.5901639344262, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI38505220, Length=491, Percent_Identity=23.6252545824847, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI45580730, Length=375, Percent_Identity=28, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI42544132, Length=394, Percent_Identity=25.3807106598985, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI122937307, Length=381, Percent_Identity=27.0341207349081, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI115511026, Length=478, Percent_Identity=23.8493723849372, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI28416953, Length=569, Percent_Identity=22.1441124780316, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI27477105, Length=509, Percent_Identity=23.3791748526523, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI156151445, Length=518, Percent_Identity=21.2355212355212, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786801, Length=522, Percent_Identity=31.2260536398467, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1788107, Length=370, Percent_Identity=27.2972972972973, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786810, Length=529, Percent_Identity=26.2759924385633, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI145693145, Length=383, Percent_Identity=26.1096605744125, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789201, Length=372, Percent_Identity=29.3010752688172, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17557194, Length=397, Percent_Identity=26.1964735516373, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=394, Percent_Identity=25.1269035532995, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=416, Percent_Identity=24.7596153846154, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32564422, Length=430, Percent_Identity=26.2790697674419, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32564420, Length=430, Percent_Identity=26.2790697674419, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=391, Percent_Identity=24.2966751918159, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556356, Length=358, Percent_Identity=27.0949720670391, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17550940, Length=354, Percent_Identity=25.7062146892655, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=21.2007504690432, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=21.2007504690432, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=21.2007504690432, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=366, Percent_Identity=23.7704918032787, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=542, Percent_Identity=25.2767527675277, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319699, Length=385, Percent_Identity=27.012987012987, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319264, Length=411, Percent_Identity=26.5206812652068, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648676, Length=483, Percent_Identity=28.1573498964803, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24581924, Length=534, Percent_Identity=23.7827715355805, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355181, Length=547, Percent_Identity=22.3034734917733, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI18859661, Length=387, Percent_Identity=24.8062015503876, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356947, Length=529, Percent_Identity=21.7391304347826, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648260, Length=395, Percent_Identity=22.7848101265823, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 60446; Mature: 60446

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIEFNQMLEVVPPCQSEPNQAHATSQLHSGFLQSVQRYPNNIALTIGNKQYDYVKLYTV
CEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHH
AQRWAFALRQSTKPLHRVGIFAYRSEAAYIGILASLLAGATFVPLNYNFPLQRTQAMIEQ
HHHHHHHHHHCCCCHHHHEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
AELDAIIVDHQSYDQFLQLADSLPVLPPCVLLPDCLRAPLLDTMIYTQAELAELPTDHEP
HCCCEEEECCCCHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VTVPPEAIAYLLFTSGSTGNPKGVPISHANVAHFLKVNQARYQITPADRLSQTFDQTFDL
EECCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHEEECCCEEEECCHHHHHHHHHHHHHH
AIFDLFMAWNHGAAVCVIQPIQLLSPFRLIEEQGITIWFSVPSVAALLRKQKLLKPNSLP
HHHHHHHHHCCCCEEEEECCHHHHCHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCC
NLRLSLFCGEALPKATAEAWQLAAPNSIIDNLYGPTELTIACAVYRWNSLTSPAECLNEV
CCEEEEEECCCCCHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHH
VPIGKLYPGLTAVVVDANDNPVPAGTEGELCVAGPQTFQGYWHNPSLTEQRFLRSKQLNG
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHCCCC
EELGYYRTGDRVVCRTNGSMIYLGRSDQQIKVHGYRVELSEIEGALLLQPGVVAAVALGW
CCCCEEECCCEEEEEECCCEEEEECCCCEEEEEEEEEEHHHCCCEEEECCCHHEEEEECC
PLENGSASGIVAFVIAPSIAVSDLQQAVQPLLPSYMLPRTIYQLETMPLNANGKIDRLAL
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCHHHHHH
ARHLAGEGTA
HHHHCCCCCC
>Mature Secondary Structure
MMIEFNQMLEVVPPCQSEPNQAHATSQLHSGFLQSVQRYPNNIALTIGNKQYDYVKLYTV
CEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHH
AQRWAFALRQSTKPLHRVGIFAYRSEAAYIGILASLLAGATFVPLNYNFPLQRTQAMIEQ
HHHHHHHHHHCCCCHHHHEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
AELDAIIVDHQSYDQFLQLADSLPVLPPCVLLPDCLRAPLLDTMIYTQAELAELPTDHEP
HCCCEEEECCCCHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VTVPPEAIAYLLFTSGSTGNPKGVPISHANVAHFLKVNQARYQITPADRLSQTFDQTFDL
EECCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHEEECCCEEEECCHHHHHHHHHHHHHH
AIFDLFMAWNHGAAVCVIQPIQLLSPFRLIEEQGITIWFSVPSVAALLRKQKLLKPNSLP
HHHHHHHHHCCCCEEEEECCHHHHCHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCC
NLRLSLFCGEALPKATAEAWQLAAPNSIIDNLYGPTELTIACAVYRWNSLTSPAECLNEV
CCEEEEEECCCCCHHHHHHHCCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHH
VPIGKLYPGLTAVVVDANDNPVPAGTEGELCVAGPQTFQGYWHNPSLTEQRFLRSKQLNG
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHCCCC
EELGYYRTGDRVVCRTNGSMIYLGRSDQQIKVHGYRVELSEIEGALLLQPGVVAAVALGW
CCCCEEECCCEEEEEECCCEEEEECCCCEEEEEEEEEEHHHCCCEEEECCCHHEEEEECC
PLENGSASGIVAFVIAPSIAVSDLQQAVQPLLPSYMLPRTIYQLETMPLNANGKIDRLAL
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCHHHHHH
ARHLAGEGTA
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9352938; 10801488 [H]