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The map label for this gene is pcm

Identifier: 159898449

GI number: 159898449

Start: 2322186

End: 2322860

Strand: Reverse

Name: pcm

Synonym: Haur_1925

Alternate gene names: 159898449

Gene position: 2322860-2322186 (Counterclockwise)

Preceding gene: 159898450

Following gene: 159898448

Centisome position: 36.6

GC content: 53.48

Gene sequence:

>675_bases
ATGAGCGACGTTTGGCAACAGCAACGTCAGCGCATGGTTGACGAACAACTTCGCCCACGAGGCATTCATGATCAGCGGAT
TTTGGCGGCAATGGCCAATGTGCCACGCCATTTGTTCGTGCCAGAAGCCTTGCAAGCCCAAGCCTATAGCGACCAAGCAC
TGCCATTAACCTTGGGCCAGACCATCTCGCAGCCATATATTGTGGCGTTGATGGCCCAGGAATTGTTGCTAAATCCTCAT
GAACAACTACTTGAAATTGGCGCTGGTTCAGGCTATGCTGCCGCAGTATTCGCCGAATTGGTACGCAAGGTCGTGACGAT
TGAGCGTCATCAAGCACTAGCTCAACAAACCCAAGTTCGGCTTAGAAATCTTGGTTATGTCAATATTGAGGTAGTCTGGG
GCGATGGCTCATTGGGCTATCCAACAGCAGCGCCTTATCATGCCATCAGCATTCCGGCGGCTACGCCCCAACTAGCCCAA
ACGCTGCTCAGCCAGTTGCACGATGGCGGGCGCTTGGTTGCCCCGATTGGCGATGCGCAGGATCAACAATTGATTCGGCT
ACAACGGCAGGGCCAAAATTGGCAAAAAACCACGATTAGCAATGTTCGCTTTGTGCCGCTGATTGGCGCTGGTGGCTGGG
AGCACGCACCAGAAACCACAGCGGAAGGAGAGTAG

Upstream 100 bases:

>100_bases
CAATTACCGGAGCCGAGGGTAATATCGAATTTTTGGGCTATTTTCAACGCAACGCGGTTGCAACAATTGCGCTGCCGCAA
GCGTTAATTGGGCTAGGTTT

Downstream 100 bases:

>100_bases
GCTCAATGTTTAATCCACAGGCTGGCTATCCATTTCGCTCAGATAACAAACCCCGCAACGAACTTTTTGAATTTGCCAAG
GCTTGGCTGGGCACAACCCT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSDVWQQQRQRMVDEQLRPRGIHDQRILAAMANVPRHLFVPEALQAQAYSDQALPLTLGQTISQPYIVALMAQELLLNPH
EQLLEIGAGSGYAAAVFAELVRKVVTIERHQALAQQTQVRLRNLGYVNIEVVWGDGSLGYPTAAPYHAISIPAATPQLAQ
TLLSQLHDGGRLVAPIGDAQDQQLIRLQRQGQNWQKTTISNVRFVPLIGAGGWEHAPETTAEGE

Sequences:

>Translated_224_residues
MSDVWQQQRQRMVDEQLRPRGIHDQRILAAMANVPRHLFVPEALQAQAYSDQALPLTLGQTISQPYIVALMAQELLLNPH
EQLLEIGAGSGYAAAVFAELVRKVVTIERHQALAQQTQVRLRNLGYVNIEVVWGDGSLGYPTAAPYHAISIPAATPQLAQ
TLLSQLHDGGRLVAPIGDAQDQQLIRLQRQGQNWQKTTISNVRFVPLIGAGGWEHAPETTAEGE
>Mature_223_residues
SDVWQQQRQRMVDEQLRPRGIHDQRILAAMANVPRHLFVPEALQAQAYSDQALPLTLGQTISQPYIVALMAQELLLNPHE
QLLEIGAGSGYAAAVFAELVRKVVTIERHQALAQQTQVRLRNLGYVNIEVVWGDGSLGYPTAAPYHAISIPAATPQLAQT
LLSQLHDGGRLVAPIGDAQDQQLIRLQRQGQNWQKTTISNVRFVPLIGAGGWEHAPETTAEGE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=205, Percent_Identity=29.7560975609756, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1789100, Length=194, Percent_Identity=45.360824742268, Blast_Score=165, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71983477, Length=207, Percent_Identity=28.0193236714976, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193207222, Length=205, Percent_Identity=26.8292682926829, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17981723, Length=210, Percent_Identity=30.952380952381, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_HERA2 (A9AUP1)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001544696.1
- ProteinModelPortal:   A9AUP1
- SMR:   A9AUP1
- GeneID:   5733814
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_1925
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- BioCyc:   HAUR316274:HAUR_1925-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24685; Mature: 24554

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 63-63

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVWQQQRQRMVDEQLRPRGIHDQRILAAMANVPRHLFVPEALQAQAYSDQALPLTLGQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEHHH
TISQPYIVALMAQELLLNPHEQLLEIGAGSGYAAAVFAELVRKVVTIERHQALAQQTQVR
HHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLGYVNIEVVWGDGSLGYPTAAPYHAISIPAATPQLAQTLLSQLHDGGRLVAPIGDAQ
HHHCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCC
DQQLIRLQRQGQNWQKTTISNVRFVPLIGAGGWEHAPETTAEGE
HHHHHHHHHCCCCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDVWQQQRQRMVDEQLRPRGIHDQRILAAMANVPRHLFVPEALQAQAYSDQALPLTLGQ
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEHHH
TISQPYIVALMAQELLLNPHEQLLEIGAGSGYAAAVFAELVRKVVTIERHQALAQQTQVR
HHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLGYVNIEVVWGDGSLGYPTAAPYHAISIPAATPQLAQTLLSQLHDGGRLVAPIGDAQ
HHHCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCC
DQQLIRLQRQGQNWQKTTISNVRFVPLIGAGGWEHAPETTAEGE
HHHHHHHHHCCCCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA