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The map label for this gene is dhbE [H]

Identifier: 159898327

GI number: 159898327

Start: 2092256

End: 2093884

Strand: Direct

Name: dhbE [H]

Synonym: Haur_1803

Alternate gene names: 159898327

Gene position: 2092256-2093884 (Clockwise)

Preceding gene: 159898326

Following gene: 159898328

Centisome position: 32.97

GC content: 54.57

Gene sequence:

>1629_bases
ATGGCTGAGCTGACGCTTGGCCTTGAAGGCACAACTGCTTGGCCCGCCGAGTTTGCCGAACGCTATCGTAAGGCTGGCTA
TTGGGTTGATCAGACCTTTGGCGAAGCACTACGCGAGTGGGCGCAACGCTCGGGCGATGCCACCGCTGTGGTATGTGGTG
AACGCCGTTGGAGCTACCGCGAGCTTGATCAACGGGTTGATCGCTTGGCAGCGGGCTTGCAACAGCTCGGCATCCAAACA
AAACAACGGGTGGTGGTGCAATTGCCCAATTGCGCCGAATGGTTTGTGGTTTGTTTTGCACTCTTTCGGGTTGGTGCAAT
TCCGTTGATGGCACTGCCCGCCCATCGCTTGGCTGAAATTGGCTATTTTTGCCAACACAGCGAAGCCGTGGCCTATGTGA
TTGCCGATAAAGTTGGCAGTTTCGATTATCGCAATTTGGCAGCTGAGGTCAAAGCGGTTGCGCCAACCTTGGAACATGTG
TTGGTGGTTGGTGAGGCTGGGCCGTTTACTGCTTTGGCTGAGGTCGATGCTGAGCCAAGCGAATTTCCAACGCTTGACCC
TGCCGAGGTGGCTTTGTTCCAGCTTTCGGGCGGTAGCACGGGCGTGCCCAAATTGATTGCCCGCACCCACGACGATTATT
TGTATTCGGTGCGAGCGAGTGCTGAAATCTGCAAGTTGGATGCCAGCAGCGTGTATTTGTGCGTCTTGCCGATGGCTCAT
AACTTTCCGATGAGTTCGCCTGGAACATTGGGAACTTTGGCGGCGGGCGGCACGGTGGTGCTTGCGCCGCAACCAAGCCC
CGATGTGGCTTTTCCCTTGATTGCTCGCGAAGGTGTGACGATTACTGGCATGGTTCCGCCGCTGGCATTGCTGTGGCTCG
ATGCTGCGGCAAATCGCAAGGCCGAATTATCAAGCCTCAAGCAAATTTTGGTTGGTGGTGCGCCCTTTGGTGCTTATACC
GCCCGCCGCGTGCAGCCAGAGCTTGGTTGCCAATTGCAACAAGTCTATGGTATGGCTGAGGGCTTGGTTAATTACACACG
GCTTGATGATGCGGCTGAGCTGATTTGCCACACCCAAGGCCGACCAATTTCGCCCTTGGATGAGGTACGGATTGTCGATG
ATGAAGATAATGATGTGCCGTTGGGCGAGCTTGGTCACCTGATTACCCGTGGCCCCTACACGATTCGCGGCTATTATCGC
GCTGCTGAACATAATCAACGGGCTTTTACCAGCGACGGTTTTTATCGGACTGGTGATTTGGCACGCTTGAACGCCACTGG
CTATGTTTCAGTCGAAGGCCGCGCCAAAGATCAAATCAATCGTGGTGGCGAAAAAGTCGCTGCCGAAGAAATCGAGCAAC
ATTTGCTCAATCACCCAGCGATTCACGATGTGGCGCTGGTGGGCTTGCCCGACCGATTTTTGGGCGAACGCACTTGTGCG
GTGATTGTCAGCAACGGTGTGAATATCAACCGCCGCGAAGTGTTGCAATTTTTGCGCAGCCGTGGGCTTGCCGAATACAA
ATTGCCAGATCGGGTTGAAATCGTGGAGAGTTTGCCCAAAACTGGGGTTGGCAAGATCAACAAACGGCTGTTACGTGAGC
AATTAAGTGCTGGTCGTGTGCCAGCCTAG

Upstream 100 bases:

>100_bases
TACTAGGCTCGACTCCTGAAGCCGAGTTGGCCGAAACTGCGGCGAAATTCCGCACGATGTTGTTGGCGATGGGCATCGAT
AGCGAAGGCGAGGTGGCCTA

Downstream 100 bases:

>100_bases
AAGGATAGATTATGGCGTTACCATCAATTGCGGCCTATGCAATTCCAACCGAATTACCAACCAACCGCGCTAATTGGGCG
GCTGAGCCACAGCGCGTAGC

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: diphosphate; (2,3-dihydroxybenzoyl)adenylate

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAELTLGLEGTTAWPAEFAERYRKAGYWVDQTFGEALREWAQRSGDATAVVCGERRWSYRELDQRVDRLAAGLQQLGIQT
KQRVVVQLPNCAEWFVVCFALFRVGAIPLMALPAHRLAEIGYFCQHSEAVAYVIADKVGSFDYRNLAAEVKAVAPTLEHV
LVVGEAGPFTALAEVDAEPSEFPTLDPAEVALFQLSGGSTGVPKLIARTHDDYLYSVRASAEICKLDASSVYLCVLPMAH
NFPMSSPGTLGTLAAGGTVVLAPQPSPDVAFPLIAREGVTITGMVPPLALLWLDAAANRKAELSSLKQILVGGAPFGAYT
ARRVQPELGCQLQQVYGMAEGLVNYTRLDDAAELICHTQGRPISPLDEVRIVDDEDNDVPLGELGHLITRGPYTIRGYYR
AAEHNQRAFTSDGFYRTGDLARLNATGYVSVEGRAKDQINRGGEKVAAEEIEQHLLNHPAIHDVALVGLPDRFLGERTCA
VIVSNGVNINRREVLQFLRSRGLAEYKLPDRVEIVESLPKTGVGKINKRLLREQLSAGRVPA

Sequences:

>Translated_542_residues
MAELTLGLEGTTAWPAEFAERYRKAGYWVDQTFGEALREWAQRSGDATAVVCGERRWSYRELDQRVDRLAAGLQQLGIQT
KQRVVVQLPNCAEWFVVCFALFRVGAIPLMALPAHRLAEIGYFCQHSEAVAYVIADKVGSFDYRNLAAEVKAVAPTLEHV
LVVGEAGPFTALAEVDAEPSEFPTLDPAEVALFQLSGGSTGVPKLIARTHDDYLYSVRASAEICKLDASSVYLCVLPMAH
NFPMSSPGTLGTLAAGGTVVLAPQPSPDVAFPLIAREGVTITGMVPPLALLWLDAAANRKAELSSLKQILVGGAPFGAYT
ARRVQPELGCQLQQVYGMAEGLVNYTRLDDAAELICHTQGRPISPLDEVRIVDDEDNDVPLGELGHLITRGPYTIRGYYR
AAEHNQRAFTSDGFYRTGDLARLNATGYVSVEGRAKDQINRGGEKVAAEEIEQHLLNHPAIHDVALVGLPDRFLGERTCA
VIVSNGVNINRREVLQFLRSRGLAEYKLPDRVEIVESLPKTGVGKINKRLLREQLSAGRVPA
>Mature_541_residues
AELTLGLEGTTAWPAEFAERYRKAGYWVDQTFGEALREWAQRSGDATAVVCGERRWSYRELDQRVDRLAAGLQQLGIQTK
QRVVVQLPNCAEWFVVCFALFRVGAIPLMALPAHRLAEIGYFCQHSEAVAYVIADKVGSFDYRNLAAEVKAVAPTLEHVL
VVGEAGPFTALAEVDAEPSEFPTLDPAEVALFQLSGGSTGVPKLIARTHDDYLYSVRASAEICKLDASSVYLCVLPMAHN
FPMSSPGTLGTLAAGGTVVLAPQPSPDVAFPLIAREGVTITGMVPPLALLWLDAAANRKAELSSLKQILVGGAPFGAYTA
RRVQPELGCQLQQVYGMAEGLVNYTRLDDAAELICHTQGRPISPLDEVRIVDDEDNDVPLGELGHLITRGPYTIRGYYRA
AEHNQRAFTSDGFYRTGDLARLNATGYVSVEGRAKDQINRGGEKVAAEEIEQHLLNHPAIHDVALVGLPDRFLGERTCAV
IVSNGVNINRREVLQFLRSRGLAEYKLPDRVEIVESLPKTGVGKINKRLLREQLSAGRVPA

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=26.7527675276753, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=26.5346534653465, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI115511026, Length=532, Percent_Identity=27.0676691729323, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI187761345, Length=540, Percent_Identity=28.3333333333333, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI187761343, Length=540, Percent_Identity=28.3333333333333, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=26.0952380952381, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI157311624, Length=504, Percent_Identity=25.9920634920635, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157311622, Length=504, Percent_Identity=25.9920634920635, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI42544132, Length=520, Percent_Identity=23.2692307692308, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI58082049, Length=505, Percent_Identity=25.7425742574257, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI28416953, Length=539, Percent_Identity=26.9016697588126, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI8923896, Length=546, Percent_Identity=21.7948717948718, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI195947378, Length=447, Percent_Identity=23.0425055928412, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI253970489, Length=380, Percent_Identity=24.4736842105263, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1786810, Length=532, Percent_Identity=49.0601503759398, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=30.1707779886148, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=27.6119402985075, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI221142682, Length=527, Percent_Identity=25.9962049335863, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790505, Length=554, Percent_Identity=26.3537906137184, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786529, Length=563, Percent_Identity=25.044404973357, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789201, Length=352, Percent_Identity=26.1363636363636, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788595, Length=498, Percent_Identity=24.0963855421687, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1786801, Length=452, Percent_Identity=24.3362831858407, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=565, Percent_Identity=28.4955752212389, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=26.865671641791, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=544, Percent_Identity=25.1838235294118, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=537, Percent_Identity=26.0707635009311, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=544, Percent_Identity=25.1838235294118, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=544, Percent_Identity=25.1838235294118, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=535, Percent_Identity=22.6168224299065, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=384, Percent_Identity=26.8229166666667, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=489, Percent_Identity=21.881390593047, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=534, Percent_Identity=25.0936329588015, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=497, Percent_Identity=21.9315895372233, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=351, Percent_Identity=23.9316239316239, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=531, Percent_Identity=21.8455743879473, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=544, Percent_Identity=24.2647058823529, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71983001, Length=544, Percent_Identity=24.2647058823529, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=366, Percent_Identity=26.775956284153, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323182, Length=547, Percent_Identity=24.3144424131627, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=540, Percent_Identity=22.4074074074074, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581924, Length=546, Percent_Identity=27.4725274725275, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=25.8979206049149, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI18859661, Length=519, Percent_Identity=24.8554913294798, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922652, Length=538, Percent_Identity=24.907063197026, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=25.7407407407407, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24667955, Length=536, Percent_Identity=25.5597014925373, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667959, Length=387, Percent_Identity=26.8733850129199, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648253, Length=483, Percent_Identity=22.5672877846791, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356947, Length=363, Percent_Identity=21.2121212121212, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648260, Length=345, Percent_Identity=22.8985507246377, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648255, Length=485, Percent_Identity=22.8865979381443, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24656500, Length=366, Percent_Identity=24.3169398907104, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI20130357, Length=350, Percent_Identity=24, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=242, Percent_Identity=24.3801652892562, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356441, Length=346, Percent_Identity=22.2543352601156, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653035, Length=263, Percent_Identity=25.8555133079848, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648257, Length=373, Percent_Identity=23.3243967828418, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58

Molecular weight: Translated: 59098; Mature: 58967

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTLGLEGTTAWPAEFAERYRKAGYWVDQTFGEALREWAQRSGDATAVVCGERRWSYR
CCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
ELDQRVDRLAAGLQQLGIQTKQRVVVQLPNCAEWFVVCFALFRVGAIPLMALPAHRLAEI
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHH
GYFCQHSEAVAYVIADKVGSFDYRNLAAEVKAVAPTLEHVLVVGEAGPFTALAEVDAEPS
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCC
EFPTLDPAEVALFQLSGGSTGVPKLIARTHDDYLYSVRASAEICKLDASSVYLCVLPMAH
CCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCHHEEECCCCEEEEEEECCC
NFPMSSPGTLGTLAAGGTVVLAPQPSPDVAFPLIAREGVTITGMVPPLALLWLDAAANRK
CCCCCCCCCCEEECCCCEEEECCCCCCCCCEEEEECCCEEEEECHHHHHHHHHHHHCCCH
AELSSLKQILVGGAPFGAYTARRVQPELGCQLQQVYGMAEGLVNYTRLDDAAELICHTQG
HHHHHHHHHHHCCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPISPLDEVRIVDDEDNDVPLGELGHLITRGPYTIRGYYRAAEHNQRAFTSDGFYRTGDL
CCCCCHHCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCE
ARLNATGYVSVEGRAKDQINRGGEKVAAEEIEQHLLNHPAIHDVALVGLPDRFLGERTCA
EEECCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHEEEECCCHHHHCCCEEE
VIVSNGVNINRREVLQFLRSRGLAEYKLPDRVEIVESLPKTGVGKINKRLLREQLSAGRV
EEEECCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
PA
CC
>Mature Secondary Structure 
AELTLGLEGTTAWPAEFAERYRKAGYWVDQTFGEALREWAQRSGDATAVVCGERRWSYR
CEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
ELDQRVDRLAAGLQQLGIQTKQRVVVQLPNCAEWFVVCFALFRVGAIPLMALPAHRLAEI
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHH
GYFCQHSEAVAYVIADKVGSFDYRNLAAEVKAVAPTLEHVLVVGEAGPFTALAEVDAEPS
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCC
EFPTLDPAEVALFQLSGGSTGVPKLIARTHDDYLYSVRASAEICKLDASSVYLCVLPMAH
CCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCHHEEECCCCEEEEEEECCC
NFPMSSPGTLGTLAAGGTVVLAPQPSPDVAFPLIAREGVTITGMVPPLALLWLDAAANRK
CCCCCCCCCCEEECCCCEEEECCCCCCCCCEEEEECCCEEEEECHHHHHHHHHHHHCCCH
AELSSLKQILVGGAPFGAYTARRVQPELGCQLQQVYGMAEGLVNYTRLDDAAELICHTQG
HHHHHHHHHHHCCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPISPLDEVRIVDDEDNDVPLGELGHLITRGPYTIRGYYRAAEHNQRAFTSDGFYRTGDL
CCCCCHHCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCE
ARLNATGYVSVEGRAKDQINRGGEKVAAEEIEQHLLNHPAIHDVALVGLPDRFLGERTCA
EEECCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHHEEEECCCHHHHCCCEEE
VIVSNGVNINRREVLQFLRSRGLAEYKLPDRVEIVESLPKTGVGKINKRLLREQLSAGRV
EEEECCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
PA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 2,3-dihydroxybenzoate

Specific reaction: ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]