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The map label for this gene is fruB [H]

Identifier: 159898052

GI number: 159898052

Start: 1779653

End: 1780126

Strand: Direct

Name: fruB [H]

Synonym: Haur_1527

Alternate gene names: 159898052

Gene position: 1779653-1780126 (Clockwise)

Preceding gene: 159898051

Following gene: 159898053

Centisome position: 28.04

GC content: 52.53

Gene sequence:

>474_bases
ATGGCAATCTTATCGAGCGAAACGATTCGGCTAGGAGCTAGCGCTGCTAGCAAACAAGAGGCGATTCAGCAGGCCGGAGC
GTTGTTAGTTGCCAGTGGTTGTGTCGCTGCCAACTATGTCGATGGTATGTTGAAACGCGAACAAGTTATCTCAACCTATC
TGGGCAATGGGGTCGCAATTCCCCATGGTCAGCACGAAAATATGGCCGATGTGATTCGCACTGGCATTGCTGTGGTGCAG
TTTCCCGCTGGCGTTGAGTGGGAGCCAGGCGAATTGGCCTATTTGGTGATTGGGATTGCTGCCAACGCCGATGAGCATGT
GGGTGTGTTAGCAAATTTGGCCGAAGTGATCGAGGATGAGGCGATTGTGCAGCAATTGGCTCAAACTAGCGATCCATCGC
AGATTCTTGAGCAATTAGGGCGAGCGCGTGAGGAAGAAGCCTACGACGAGGAAAGGCATCATGCAGGAGCTTGA

Upstream 100 bases:

>100_bases
AATGCCGTCGTGATTGCCTTTACCCAATTCCTCAATGATCCTGTGTTTGATCAAGTGGTTCAAAGCTTGCAGGATGGCAC
TGATCTTGTGGAGGCCTAAA

Downstream 100 bases:

>100_bases
TTTGGTCATTCATAACTCGGCAGGCCTGCATGCGCGGCCTGCCCGAGTCTTAGTTGACCTTGCAAAACAATTCAAATCGA
CGATTTCGATTCGTGCTGGC

Product: phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAILSSETIRLGASAASKQEAIQQAGALLVASGCVAANYVDGMLKREQVISTYLGNGVAIPHGQHENMADVIRTGIAVVQ
FPAGVEWEPGELAYLVIGIAANADEHVGVLANLAEVIEDEAIVQQLAQTSDPSQILEQLGRAREEEAYDEERHHAGA

Sequences:

>Translated_157_residues
MAILSSETIRLGASAASKQEAIQQAGALLVASGCVAANYVDGMLKREQVISTYLGNGVAIPHGQHENMADVIRTGIAVVQ
FPAGVEWEPGELAYLVIGIAANADEHVGVLANLAEVIEDEAIVQQLAQTSDPSQILEQLGRAREEEAYDEERHHAGA
>Mature_156_residues
AILSSETIRLGASAASKQEAIQQAGALLVASGCVAANYVDGMLKREQVISTYLGNGVAIPHGQHENMADVIRTGIAVVQF
PAGVEWEPGELAYLVIGIAANADEHVGVLANLAEVIEDEAIVQQLAQTSDPSQILEQLGRAREEEAYDEERHHAGA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=126, Percent_Identity=50.7936507936508, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1790027, Length=141, Percent_Identity=43.9716312056738, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16599; Mature: 16468

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILSSETIRLGASAASKQEAIQQAGALLVASGCVAANYVDGMLKREQVISTYLGNGVAI
CCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEC
PHGQHENMADVIRTGIAVVQFPAGVEWEPGELAYLVIGIAANADEHVGVLANLAEVIEDE
CCCCCCCHHHHHHHHHHHEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
AIVQQLAQTSDPSQILEQLGRAREEEAYDEERHHAGA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AILSSETIRLGASAASKQEAIQQAGALLVASGCVAANYVDGMLKREQVISTYLGNGVAI
CCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCEEC
PHGQHENMADVIRTGIAVVQFPAGVEWEPGELAYLVIGIAANADEHVGVLANLAEVIEDE
CCCCCCCHHHHHHHHHHHEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
AIVQQLAQTSDPSQILEQLGRAREEEAYDEERHHAGA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]