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The map label for this gene is fruB(HI) [H]

Identifier: 159898053

GI number: 159898053

Start: 1780113

End: 1782224

Strand: Direct

Name: fruB(HI) [H]

Synonym: Haur_1528

Alternate gene names: 159898053

Gene position: 1780113-1782224 (Clockwise)

Preceding gene: 159898052

Following gene: 159898054

Centisome position: 28.05

GC content: 55.73

Gene sequence:

>2112_bases
ATGCAGGAGCTTGATTTGGTCATTCATAACTCGGCAGGCCTGCATGCGCGGCCTGCCCGAGTCTTAGTTGACCTTGCAAA
ACAATTCAAATCGACGATTTCGATTCGTGCTGGCGGCAAGCGGGTCAATGCCAAAAGCATGATCGCGCTGTTGACCCTCG
GCGTAGTCTGTGGTCAGGCGATCCAAATCGAGATTAACGGCGAAGATGAAGCCGCCGCTGCCGAGGCGATTACAACTGCG
GTGCACGAAGGTTTGGGCGAAGGTCATGGCACACCAGCCGCCAATAGCGTTAATGATGCTTTGGTTGCGGCTCGCAATGG
CCATGCCAATCTCAATGGCCATGCCAAGCTTGAAACCACCGTTGCTGTGGCCGAACCGCCGCCTGCGCCAGCCCCAACTC
GCGCTGAGCCACTCAAAGCTGGCGCGATCATCCAAGGGATTGCTGGCGCACCTGGCATTGCGGTTGGCACAATTTTGCGC
TACGAACGTGCGCGAATCGAAATTAGCCACCGATTTAGTGGGGTGGATAACGAACTCCAGCGTTTGCAAGCGGCCTTGAC
CACCGCCCAACAGCAATTGGTGGCGCTGCGCGAACAGGTATTGTTACGGGCTGATGCCAGCGAAGCCGCCATTTTTGATG
TTCACCGCGATATTTTGGCCGATCCTGCCTTGCTTGAGGCTGTGCATGGCTCAATTGGTGCTGGGCAAGGGGCCGAAGTC
GCTTGGCAACAGGTGATCAATCAACAGGCTAACGCGATTGCTCAACTCAACGATGCCCTACTTGCCGAGCGTTCTAGCGA
TATTCGTGATGTAGGCGATCGGGTGCTGCGCCTTTTGGTGGGAGCCGAAGCTTCGACGCTTGATGCCCACTGGGCAAAGG
CCAATCAGCCGATGATTGTCGTGGCCTATGATCTGACACCTTCAGAAACCGCTGCTTTTGATCCAGCCAAAGTGCTGGGT
TTTTGCACAGCGGTAGGTGGCCCAAATGCCCACACCGCGATTTTGGCTCGTGCCTTGGGTTTACCAGCGGTGATTAGTGC
TGGCCCAAGCGTCCTTGAACTTGCCACCGGAACCGAAGTAATTCTCGATGGTACGGCTGGCACCTTGTTGATCTGCCCAG
CGCCTGAAGCCATTGTGGCGGCCAAAACTGCCCAGCAGCGCGAGCGTGAGCATCAAGCATGGGCTATGCGCAGCGCTAAC
GAGCCAGCCACAACCGTTGATGGTCAGCATATCGAAGTGGTTGCTAACATTGGTGGCCTGAGCGAAGCTCAGCAAGCCAC
AACTTTAGGTGCTGATGGGGTGGGTTTGTTGCGCACCGAATTTTTGTTCTTGGAGCGCACCCAAGCCCCAACCGAAGATG
AGCAGTTTGCAACCTACCGCGAAATTGCCCAAGCCATGGGCGATGCCCCAGTGATTGTGCGCACCTTGGATATTGGCGGC
GACAAGCCACTGCCCTATCTGGCCTTGCCTGCTGAAGAAAACCCATTTTTGGGCGAACGTGGCATTCGCTTGTGCCTAGC
ACACCCTGAATTGCTACAAACCCAATTACGGGCAATTTTACGTGCTGCCAGCTTTGGCCGCTTGCGAATTATGTTCCCAA
TGATTGCCGATGCTGGTGAGTTACGCGCTGCCAAAGCCGAAATCGAGCGGATTCGCAACGAATTGCAAGTAGCTCCAATC
GAAATCGGGATTATGATCGAAGTGCCTTCAGCCGCTTTGATGACCGATATTTTGGCGGCTGAAGTTGATTTCTTCTCAAT
TGGCACCAACGACCTGACCCAATACACCTTAGCTATGGATCGGACGCACCCGACGCTGGCGGCTCAAGCCGATGGTTTGC
ATCCAGCGGTGTTGCGCCTGATTGCTCGAACGGTTGAGGCGGCCCATGCTGCTGGCAAATGGGTCGGAGTTTGCGGCGAG
TTGGGCGCTGATCCGCAAGCTGTGCCAATTTTGGTGGGCCTTGGGGTTGATGAACTCAGCGTCAGCGTGCCCGCCATTCC
AACTGTTAAAGCCCAAATCCGGGCATTGAACTTTGCTCAGTGCCAAACATCTGCTCGGCGGGCGTTGGTCTGTGCCACTG
CCGCCGAAGTACGCCAAGGAGCGTTCGACTAA

Upstream 100 bases:

>100_bases
GCGATTGTGCAGCAATTGGCTCAAACTAGCGATCCATCGCAGATTCTTGAGCAATTAGGGCGAGCGCGTGAGGAAGAAGC
CTACGACGAGGAAAGGCATC

Downstream 100 bases:

>100_bases
TGACAACGTTTGACGACTATCGATCCCCTGATGCCCCAATTGCCGCAACCACGGTTGCTTGGAATATGTATGGGGCTGGG
GTCGAACATATCGGGCGCAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MQELDLVIHNSAGLHARPARVLVDLAKQFKSTISIRAGGKRVNAKSMIALLTLGVVCGQAIQIEINGEDEAAAAEAITTA
VHEGLGEGHGTPAANSVNDALVAARNGHANLNGHAKLETTVAVAEPPPAPAPTRAEPLKAGAIIQGIAGAPGIAVGTILR
YERARIEISHRFSGVDNELQRLQAALTTAQQQLVALREQVLLRADASEAAIFDVHRDILADPALLEAVHGSIGAGQGAEV
AWQQVINQQANAIAQLNDALLAERSSDIRDVGDRVLRLLVGAEASTLDAHWAKANQPMIVVAYDLTPSETAAFDPAKVLG
FCTAVGGPNAHTAILARALGLPAVISAGPSVLELATGTEVILDGTAGTLLICPAPEAIVAAKTAQQREREHQAWAMRSAN
EPATTVDGQHIEVVANIGGLSEAQQATTLGADGVGLLRTEFLFLERTQAPTEDEQFATYREIAQAMGDAPVIVRTLDIGG
DKPLPYLALPAEENPFLGERGIRLCLAHPELLQTQLRAILRAASFGRLRIMFPMIADAGELRAAKAEIERIRNELQVAPI
EIGIMIEVPSAALMTDILAAEVDFFSIGTNDLTQYTLAMDRTHPTLAAQADGLHPAVLRLIARTVEAAHAAGKWVGVCGE
LGADPQAVPILVGLGVDELSVSVPAIPTVKAQIRALNFAQCQTSARRALVCATAAEVRQGAFD

Sequences:

>Translated_703_residues
MQELDLVIHNSAGLHARPARVLVDLAKQFKSTISIRAGGKRVNAKSMIALLTLGVVCGQAIQIEINGEDEAAAAEAITTA
VHEGLGEGHGTPAANSVNDALVAARNGHANLNGHAKLETTVAVAEPPPAPAPTRAEPLKAGAIIQGIAGAPGIAVGTILR
YERARIEISHRFSGVDNELQRLQAALTTAQQQLVALREQVLLRADASEAAIFDVHRDILADPALLEAVHGSIGAGQGAEV
AWQQVINQQANAIAQLNDALLAERSSDIRDVGDRVLRLLVGAEASTLDAHWAKANQPMIVVAYDLTPSETAAFDPAKVLG
FCTAVGGPNAHTAILARALGLPAVISAGPSVLELATGTEVILDGTAGTLLICPAPEAIVAAKTAQQREREHQAWAMRSAN
EPATTVDGQHIEVVANIGGLSEAQQATTLGADGVGLLRTEFLFLERTQAPTEDEQFATYREIAQAMGDAPVIVRTLDIGG
DKPLPYLALPAEENPFLGERGIRLCLAHPELLQTQLRAILRAASFGRLRIMFPMIADAGELRAAKAEIERIRNELQVAPI
EIGIMIEVPSAALMTDILAAEVDFFSIGTNDLTQYTLAMDRTHPTLAAQADGLHPAVLRLIARTVEAAHAAGKWVGVCGE
LGADPQAVPILVGLGVDELSVSVPAIPTVKAQIRALNFAQCQTSARRALVCATAAEVRQGAFD
>Mature_703_residues
MQELDLVIHNSAGLHARPARVLVDLAKQFKSTISIRAGGKRVNAKSMIALLTLGVVCGQAIQIEINGEDEAAAAEAITTA
VHEGLGEGHGTPAANSVNDALVAARNGHANLNGHAKLETTVAVAEPPPAPAPTRAEPLKAGAIIQGIAGAPGIAVGTILR
YERARIEISHRFSGVDNELQRLQAALTTAQQQLVALREQVLLRADASEAAIFDVHRDILADPALLEAVHGSIGAGQGAEV
AWQQVINQQANAIAQLNDALLAERSSDIRDVGDRVLRLLVGAEASTLDAHWAKANQPMIVVAYDLTPSETAAFDPAKVLG
FCTAVGGPNAHTAILARALGLPAVISAGPSVLELATGTEVILDGTAGTLLICPAPEAIVAAKTAQQREREHQAWAMRSAN
EPATTVDGQHIEVVANIGGLSEAQQATTLGADGVGLLRTEFLFLERTQAPTEDEQFATYREIAQAMGDAPVIVRTLDIGG
DKPLPYLALPAEENPFLGERGIRLCLAHPELLQTQLRAILRAASFGRLRIMFPMIADAGELRAAKAEIERIRNELQVAPI
EIGIMIEVPSAALMTDILAAEVDFFSIGTNDLTQYTLAMDRTHPTLAAQADGLHPAVLRLIARTVEAAHAAGKWVGVCGE
LGADPQAVPILVGLGVDELSVSVPAIPTVKAQIRALNFAQCQTSARRALVCATAAEVRQGAFD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=42.2338568935428, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI48994992, Length=703, Percent_Identity=34.5661450924609, Blast_Score=348, Evalue=6e-97,
Organism=Escherichia coli, GI1788726, Length=711, Percent_Identity=33.6146272855134, Blast_Score=344, Evalue=9e-96,
Organism=Escherichia coli, GI1789193, Length=577, Percent_Identity=36.2218370883882, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1787994, Length=416, Percent_Identity=26.9230769230769, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI226510935, Length=362, Percent_Identity=25.1381215469613, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 73826; Mature: 73826

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00589 PTS_HPR_SER ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQELDLVIHNSAGLHARPARVLVDLAKQFKSTISIRAGGKRVNAKSMIALLTLGVVCGQA
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCE
IQIEINGEDEAAAAEAITTAVHEGLGEGHGTPAANSVNDALVAARNGHANLNGHAKLETT
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEECCCCCCCCCCEEEEEE
VAVAEPPPAPAPTRAEPLKAGAIIQGIAGAPGIAVGTILRYERARIEISHRFSGVDNELQ
EEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHEEEEEHHCCCCHHHHH
RLQAALTTAQQQLVALREQVLLRADASEAAIFDVHRDILADPALLEAVHGSIGAGQGAEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHH
AWQQVINQQANAIAQLNDALLAERSSDIRDVGDRVLRLLVGAEASTLDAHWAKANQPMIV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
VAYDLTPSETAAFDPAKVLGFCTAVGGPNAHTAILARALGLPAVISAGPSVLELATGTEV
EEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCEEEECCCCEE
ILDGTAGTLLICPAPEAIVAAKTAQQREREHQAWAMRSANEPATTVDGQHIEVVANIGGL
EEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
SEAQQATTLGADGVGLLRTEFLFLERTQAPTEDEQFATYREIAQAMGDAPVIVRTLDIGG
CHHHHHHHCCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
DKPLPYLALPAEENPFLGERGIRLCLAHPELLQTQLRAILRAASFGRLRIMFPMIADAGE
CCCCCEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCH
LRAAKAEIERIRNELQVAPIEIGIMIEVPSAALMTDILAAEVDFFSIGTNDLTQYTLAMD
HHHHHHHHHHHHHHHEEEEEEEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHEEEEEC
RTHPTLAAQADGLHPAVLRLIARTVEAAHAAGKWVGVCGELGADPQAVPILVGLGVDELS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCEEEEEECCHHHHE
VSVPAIPTVKAQIRALNFAQCQTSARRALVCATAAEVRQGAFD
EECCCCCCHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHCCCCC
>Mature Secondary Structure
MQELDLVIHNSAGLHARPARVLVDLAKQFKSTISIRAGGKRVNAKSMIALLTLGVVCGQA
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCE
IQIEINGEDEAAAAEAITTAVHEGLGEGHGTPAANSVNDALVAARNGHANLNGHAKLETT
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEECCCCCCCCCCEEEEEE
VAVAEPPPAPAPTRAEPLKAGAIIQGIAGAPGIAVGTILRYERARIEISHRFSGVDNELQ
EEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHEEEEEHHCCCCHHHHH
RLQAALTTAQQQLVALREQVLLRADASEAAIFDVHRDILADPALLEAVHGSIGAGQGAEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHH
AWQQVINQQANAIAQLNDALLAERSSDIRDVGDRVLRLLVGAEASTLDAHWAKANQPMIV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEE
VAYDLTPSETAAFDPAKVLGFCTAVGGPNAHTAILARALGLPAVISAGPSVLELATGTEV
EEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCEEEECCCCEE
ILDGTAGTLLICPAPEAIVAAKTAQQREREHQAWAMRSANEPATTVDGQHIEVVANIGGL
EEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
SEAQQATTLGADGVGLLRTEFLFLERTQAPTEDEQFATYREIAQAMGDAPVIVRTLDIGG
CHHHHHHHCCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
DKPLPYLALPAEENPFLGERGIRLCLAHPELLQTQLRAILRAASFGRLRIMFPMIADAGE
CCCCCEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCH
LRAAKAEIERIRNELQVAPIEIGIMIEVPSAALMTDILAAEVDFFSIGTNDLTQYTLAMD
HHHHHHHHHHHHHHHEEEEEEEEEEEECCCHHHHHHHHHHHCCEEECCCCHHHHEEEEEC
RTHPTLAAQADGLHPAVLRLIARTVEAAHAAGKWVGVCGELGADPQAVPILVGLGVDELS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCEEEEEECCHHHHE
VSVPAIPTVKAQIRALNFAQCQTSARRALVCATAAEVRQGAFD
EECCCCCCHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193161 [H]