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The map label for this gene is nodL [H]

Identifier: 159897988

GI number: 159897988

Start: 1706225

End: 1706803

Strand: Direct

Name: nodL [H]

Synonym: Haur_1463

Alternate gene names: 159897988

Gene position: 1706225-1706803 (Clockwise)

Preceding gene: 159897987

Following gene: 159897989

Centisome position: 26.88

GC content: 49.91

Gene sequence:

>579_bases
ATGAGCAAAACTGAACGTGAGAAAATGTTGGCGGGTGAATTATATTCAGCCTTTGATCCTGAATTACAGGCGCTTTCGCT
ACGAAGCCGCCGCTTGAGTCGCCAATTTCATGCAATTGAGCCAGGTCAGCCAGAGCAATCGCGAGCGTTGCTGGGTGAAT
GGTTTGGGCACATTGGCGAAAATGTTTGGATCGAGCCACCATTTTTCTGTGATTACGGGGCGCACATTCGTTTGGGCAAT
AATGTCTTTTTCAACGCCAATTGTGTGATTCTCGATTGTAACTACATTACGATTGGTGACAACACGATGTGTGGCCCAGC
GGTGCAAATTTATGCTGCCAGTCATCCGCTGATCGCGAGTGAACGGATCAAAGGGCCGGAATTAGGCTTTCCGGTGACGA
TTGGCAAAAATGTTTGGATCGGCGGCGGCTCGATCATCTGCCCAGGCGTGACGATTGGCGATAACACCACGATTGGCGCT
GGTAGTGTAGTGACCAAAGATATTCCGGCGAATGTCTTTGCGGCGGGCAATCCATGTCGGGTCATTCGCGAATTGACTGA
TCCTAGTCTTAGCGAATAG

Upstream 100 bases:

>100_bases
TGTTTTGGCGCTGGCTTAATCGTTCGTAGGGCTGAATCATTTTCAAAAACGTTTCAGTATGCTAAAATTTAAACAATGTC
GTTATTGAGGATGAGCATCA

Downstream 100 bases:

>100_bases
GCGTATAATACAAAAAACGGCTTTTTCAATGCCAGCGTCAGGTTGATGCTGGCGTTGTTGTCATATTAACGAAATTACAT
GGATGCAGCTTATGCCTGAT

Product: hexapaptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSKTEREKMLAGELYSAFDPELQALSLRSRRLSRQFHAIEPGQPEQSRALLGEWFGHIGENVWIEPPFFCDYGAHIRLGN
NVFFNANCVILDCNYITIGDNTMCGPAVQIYAASHPLIASERIKGPELGFPVTIGKNVWIGGGSIICPGVTIGDNTTIGA
GSVVTKDIPANVFAAGNPCRVIRELTDPSLSE

Sequences:

>Translated_192_residues
MSKTEREKMLAGELYSAFDPELQALSLRSRRLSRQFHAIEPGQPEQSRALLGEWFGHIGENVWIEPPFFCDYGAHIRLGN
NVFFNANCVILDCNYITIGDNTMCGPAVQIYAASHPLIASERIKGPELGFPVTIGKNVWIGGGSIICPGVTIGDNTTIGA
GSVVTKDIPANVFAAGNPCRVIRELTDPSLSE
>Mature_191_residues
SKTEREKMLAGELYSAFDPELQALSLRSRRLSRQFHAIEPGQPEQSRALLGEWFGHIGENVWIEPPFFCDYGAHIRLGNN
VFFNANCVILDCNYITIGDNTMCGPAVQIYAASHPLIASERIKGPELGFPVTIGKNVWIGGGSIICPGVTIGDNTTIGAG
SVVTKDIPANVFAAGNPCRVIRELTDPSLSE

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=182, Percent_Identity=50.5494505494505, Blast_Score=184, Evalue=3e-48,
Organism=Escherichia coli, GI1786537, Length=187, Percent_Identity=45.4545454545455, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788367, Length=148, Percent_Identity=33.7837837837838, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322243, Length=182, Percent_Identity=46.7032967032967, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 20834; Mature: 20703

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTEREKMLAGELYSAFDPELQALSLRSRRLSRQFHAIEPGQPEQSRALLGEWFGHIGE
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
NVWIEPPFFCDYGAHIRLGNNVFFNANCVILDCNYITIGDNTMCGPAVQIYAASHPLIAS
CCEECCCEEECCCCEEEECCEEEEECCEEEEECCEEEECCCCCCCCEEEEEECCCCCEEC
ERIKGPELGFPVTIGKNVWIGGGSIICPGVTIGDNTTIGAGSVVTKDIPANVFAAGNPCR
CCCCCCCCCCCEEECCEEEEECCCEEECCEEECCCCEECCCCEEECCCCCCEEECCCHHH
VIRELTDPSLSE
HHHHHCCCCCCC
>Mature Secondary Structure 
SKTEREKMLAGELYSAFDPELQALSLRSRRLSRQFHAIEPGQPEQSRALLGEWFGHIGE
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
NVWIEPPFFCDYGAHIRLGNNVFFNANCVILDCNYITIGDNTMCGPAVQIYAASHPLIAS
CCEECCCEEECCCCEEEECCEEEEECCEEEEECCEEEECCCCCCCCEEEEEECCCCCEEC
ERIKGPELGFPVTIGKNVWIGGGSIICPGVTIGDNTTIGAGSVVTKDIPANVFAAGNPCR
CCCCCCCCCCCEEECCEEEEECCCEEECCEEECCCCEECCCCEEECCCCCCEEECCCHHH
VIRELTDPSLSE
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3132583; 2562395; 2615659 [H]