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The map label for this gene is dus [H]

Identifier: 159897989

GI number: 159897989

Start: 1706886

End: 1707974

Strand: Direct

Name: dus [H]

Synonym: Haur_1464

Alternate gene names: 159897989

Gene position: 1706886-1707974 (Clockwise)

Preceding gene: 159897988

Following gene: 159897990

Centisome position: 26.89

GC content: 51.7

Gene sequence:

>1089_bases
ATGCAGCTTATGCCTGATACTCTGACCCAAGCATTGCCCGAATCATTTCAACTTGGCTCAATGCGCATTTTTCCAAACAT
GGTGCTGGCCCCGATGGCTGGCGTAACCGATTCAGTCTTTCGGCGGCTGGTGTTGTCGTTGGGTGGCTGTGGCTTGGTTG
TTTCTGAGATGACCAATGCTGCCAGTGTTTCGCCCAAGGCCATGAAACGCCATCGCTTGCTGGATTATCTGCCCGAAGAA
CGGCCAATTTCGATTCAACTTTCGGGCAACGACCCCGATTTGGTGGCAACCGCCGCCCGCTTTGTTGAAGAACTTGGCCC
CGATGTAATTGACATAAACTGTGGCTGCCCTTCGCCCAAAGTCACTGGTGGCGGCCATGGTTCGGCCTTGCTCAAAGATT
TGCCCAAAATGCAGCAAATGCTCAAAGCCGTGTATGCAGCGATCAACATTCCGTTTACGCTCAAATTTCGGGCTGGCTGG
GATGAGCAATCGCTAAATTATATTGATACCGCTAAAATTGCTGAAGATGCTGGTTGTGCCGCGATTACCTTGCATCCACG
TACCAAAGTGCAAGGCTACAGCGGCGATGCCGATTGGTCGCGGGTTGCTGAAGTTGTGCAAGCAGTCTCGATTCCGGTGA
TTGGCTCAGGCGATGTGCGCACACCAGCCGATGCTTTGGCGCGTTTGGAGCAAACTGGGGTTGCAGCAGTGATGATTGGT
CGAGCTGCCATGGCCAATCCATGGATTTTTCGCCAAATTGCTCAATTGCGGGCAGGCGAACCCATATTTGTGCCAACACC
AAGCGATAAACGTGATTTGCTGGTGCGCTATGTTGATATGTGTGCTGAAACTATGGTTGAGCGCCAAGCCCTTGGCAAGC
TAAAACAACTGATTGGTCAATTCAGCATTGGTTTGTATGCTAGCAACCAATTGCGCCGCGATGTACAACGTGCCAACGAA
ATTGAAGCCGCCAAAGCGATTATTGCCAACTTTTTTGAGCCATTTATCAGCGGCGCGGTCGAGGCGGTCGAAGTGCCCGA
CGAAATCGCCGTGATCAAAGAAGGTTGCGAGAACGGCGCGAATAATTAG

Upstream 100 bases:

>100_bases
CCTAGTCTTAGCGAATAGGCGTATAATACAAAAAACGGCTTTTTCAATGCCAGCGTCAGGTTGATGCTGGCGTTGTTGTC
ATATTAACGAAATTACATGG

Downstream 100 bases:

>100_bases
GCCGAGGGATCAGAGGCTAGCGGTTAGGGGTCAGCGTCGTGCGATGAAGTCAAAAGGCAAAAGTCAACAACCTGTTATTG
TATTCGGGCAATTCGTGGCG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MQLMPDTLTQALPESFQLGSMRIFPNMVLAPMAGVTDSVFRRLVLSLGGCGLVVSEMTNAASVSPKAMKRHRLLDYLPEE
RPISIQLSGNDPDLVATAARFVEELGPDVIDINCGCPSPKVTGGGHGSALLKDLPKMQQMLKAVYAAINIPFTLKFRAGW
DEQSLNYIDTAKIAEDAGCAAITLHPRTKVQGYSGDADWSRVAEVVQAVSIPVIGSGDVRTPADALARLEQTGVAAVMIG
RAAMANPWIFRQIAQLRAGEPIFVPTPSDKRDLLVRYVDMCAETMVERQALGKLKQLIGQFSIGLYASNQLRRDVQRANE
IEAAKAIIANFFEPFISGAVEAVEVPDEIAVIKEGCENGANN

Sequences:

>Translated_362_residues
MQLMPDTLTQALPESFQLGSMRIFPNMVLAPMAGVTDSVFRRLVLSLGGCGLVVSEMTNAASVSPKAMKRHRLLDYLPEE
RPISIQLSGNDPDLVATAARFVEELGPDVIDINCGCPSPKVTGGGHGSALLKDLPKMQQMLKAVYAAINIPFTLKFRAGW
DEQSLNYIDTAKIAEDAGCAAITLHPRTKVQGYSGDADWSRVAEVVQAVSIPVIGSGDVRTPADALARLEQTGVAAVMIG
RAAMANPWIFRQIAQLRAGEPIFVPTPSDKRDLLVRYVDMCAETMVERQALGKLKQLIGQFSIGLYASNQLRRDVQRANE
IEAAKAIIANFFEPFISGAVEAVEVPDEIAVIKEGCENGANN
>Mature_362_residues
MQLMPDTLTQALPESFQLGSMRIFPNMVLAPMAGVTDSVFRRLVLSLGGCGLVVSEMTNAASVSPKAMKRHRLLDYLPEE
RPISIQLSGNDPDLVATAARFVEELGPDVIDINCGCPSPKVTGGGHGSALLKDLPKMQQMLKAVYAAINIPFTLKFRAGW
DEQSLNYIDTAKIAEDAGCAAITLHPRTKVQGYSGDADWSRVAEVVQAVSIPVIGSGDVRTPADALARLEQTGVAAVMIG
RAAMANPWIFRQIAQLRAGEPIFVPTPSDKRDLLVRYVDMCAETMVERQALGKLKQLIGQFSIGLYASNQLRRDVQRANE
IEAAKAIIANFFEPFISGAVEAVEVPDEIAVIKEGCENGANN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=260, Percent_Identity=31.1538461538462, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI239788462, Length=239, Percent_Identity=32.6359832635983, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=31.5789473684211, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=29.9107142857143, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI8923374, Length=254, Percent_Identity=29.1338582677165, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=34.5794392523364, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1788462, Length=328, Percent_Identity=29.5731707317073, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI145693211, Length=237, Percent_Identity=29.535864978903, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=258, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17507177, Length=227, Percent_Identity=32.15859030837, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=174, Percent_Identity=32.7586206896552, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=257, Percent_Identity=28.7937743190661, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=275, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=317, Percent_Identity=24.2902208201893, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585320, Length=258, Percent_Identity=29.4573643410853, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=31.7596566523605, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=31.7596566523605, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=33.0396475770925, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45549423, Length=177, Percent_Identity=32.2033898305085, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39080; Mature: 39080

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLMPDTLTQALPESFQLGSMRIFPNMVLAPMAGVTDSVFRRLVLSLGGCGLVVSEMTNA
CCCCHHHHHHHCCHHHCCCCEECCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHH
ASVSPKAMKRHRLLDYLPEERPISIQLSGNDPDLVATAARFVEELGPDVIDINCGCPSPK
HCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
VTGGGHGSALLKDLPKMQQMLKAVYAAINIPFTLKFRAGWDEQSLNYIDTAKIAEDAGCA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCE
AITLHPRTKVQGYSGDADWSRVAEVVQAVSIPVIGSGDVRTPADALARLEQTGVAAVMIG
EEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
RAAMANPWIFRQIAQLRAGEPIFVPTPSDKRDLLVRYVDMCAETMVERQALGKLKQLIGQ
HHHHCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSIGLYASNQLRRDVQRANEIEAAKAIIANFFEPFISGAVEAVEVPDEIAVIKEGCENGA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCC
NN
CC
>Mature Secondary Structure
MQLMPDTLTQALPESFQLGSMRIFPNMVLAPMAGVTDSVFRRLVLSLGGCGLVVSEMTNA
CCCCHHHHHHHCCHHHCCCCEECCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHH
ASVSPKAMKRHRLLDYLPEERPISIQLSGNDPDLVATAARFVEELGPDVIDINCGCPSPK
HCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
VTGGGHGSALLKDLPKMQQMLKAVYAAINIPFTLKFRAGWDEQSLNYIDTAKIAEDAGCA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCE
AITLHPRTKVQGYSGDADWSRVAEVVQAVSIPVIGSGDVRTPADALARLEQTGVAAVMIG
EEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
RAAMANPWIFRQIAQLRAGEPIFVPTPSDKRDLLVRYVDMCAETMVERQALGKLKQLIGQ
HHHHCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSIGLYASNQLRRDVQRANEIEAAKAIIANFFEPFISGAVEAVEVPDEIAVIKEGCENGA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCC
NN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]