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The map label for this gene is treZ [H]

Identifier: 159897808

GI number: 159897808

Start: 1488409

End: 1490091

Strand: Direct

Name: treZ [H]

Synonym: Haur_1279

Alternate gene names: 159897808

Gene position: 1488409-1490091 (Clockwise)

Preceding gene: 159897803

Following gene: 159897813

Centisome position: 23.45

GC content: 48.78

Gene sequence:

>1683_bases
ATGCAAAATGATGCCAATCCTGATGCTTCGCCAACGGTGGCTAGTTTGCCCGATGGGGTTTGGATGGGTGCACGCGATTT
AGGCAATGGCGCGGTGGCGTTTGGCCTTTATGCACCATGGAAGCAGAGTGTTCATTTAATTGGCTCGTTTAACGATTGGA
ACCAAACCGCTGATCCATTAAATATTTCGGATCGCGGGATTTGGTGGATTATCAAAGAAGGTTTAGCAGCTGGCGAATAT
GCTTATCAATTTGTGATTGACGGCGAGACGATCATCGGCGACCCCTATGCCCGTGAATTGCGTTGGGCTGGTGGCGATCA
GCCACAGGCGATTATTCATGTTGGCGAGCCAGCCTACGAGTGGCACGACGATGGTTTTGGCATTCGTCCGCTGAATGAAT
TGGTGATTTATGAATTGCATGTTGGCGATTTTAGCGAGGCTGGCACCTTCAAAGCCGTCACTGAACGAATTCCTTATCTT
CAAGATTTAGGGGTGAATGCGCTTGAATTAATGCCGTTGTTTGAGTTTCCTGGCGATCGTTCGTGGGGCTATAATCCGGC
CTATTTCTTTGCTCCCGAATCGACCTATGGCACGCCTGCCGATTTGCGCGAGTTGATCGATACGGCCCACCAACATGGCA
TTGGGGTGATTTTGGATGTAGTGTTTAATCATGTCGATCACTCTAGCCCGATTAACTATTTGTATCCCTACGATCAAAGC
CCTTTCTTTAGCAGCGACGGCAACCCGTGGGGCTTCCCCGATTTGAACCATTGGAATGAAGCAGTCAAACAATTAATCAG
CGATGTGCAAACCTATTGGCTCAGCGATATGCACATCGATGGCTTTCGCTACGATCACGCCGAGGGAATTGGCTTTGATG
GCGAGAATGGGGTTTCGTTCTTGGGCTGGCAGGCTCGCCAAATCAAACCGCATGTCTATTTGATTGCCGAAAATCTCAAA
GATTACACCACCATGGTTGATCAAACTGATATGGATTCGTCGTGGCATCGTTCGTTTCATTCGCAAATGTTTGCCAACTT
GCGCGAAGGTGATTTCGAGGGCAATCAGTATGGCAATGTTGAGGCCACGCTGGGGATTATCGATTTTCGCAATGCTGGCT
ACAACGATAATGCCCAAGCGATCAATTATCTTGAATCGCACGATGAGCAGCGGATTGTTTACGAGGCCCAAACCAACGAA
AATATCAGCCGCGAAACCGCTTATTTGAAATCGCGACTGGGCGCGATTGTGCTATTTACCGCTGCTGGTGTGCCCATGCT
ATATCATGGCCAAGAATTTGGCATGGATACCGAACGTACAATCGACAAAAATATGCTGAAATGGGAGTTATTGCAAACAG
CTGAGGGCACTGATTTGCACGCCTTCTATCGTGGCATGATTCAATTACGTACCTCATCGAAGGCCTTGGTTGGCAATAAT
ATCCGGCCTGTCGCCATGGATGCTGAGCGCAAATTGTTGGCCTACTATCGCTGGGCCGACGATGGCAGCGAACATGTGAT
TGTTGTGGTGAATTTTGGCATCACCCTGCAATACCTTGATGTGCATTTCCCGGTTGGTGGTTTATGGCACGAATGGGTCT
ATAACTACGATCGTGAAACCCCTGAAGGTATGGCCACAATCGAAGTGCCTGGCTCAGGTGGGAAGGTCTTTGTGTTGCGC
TAA

Upstream 100 bases:

>100_bases
AAAGCCTCCATTTGTGGCATAGACTCTGCTATGTGGGGTTTGGAAATTATTAATCTAGAGTGGTAGCTAGTGATTTAGTT
AGTCGATGAGGTTCACATAT

Downstream 100 bases:

>100_bases
ACAGGAGCACGCCCTCACCCCCTGACCCCCGCTCCCGCCCAGCGAGTGAGGGGGAACCGATGATCATGAAGGATGAATGC
CCCTCGCCCGCCGCCGTGGG

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MQNDANPDASPTVASLPDGVWMGARDLGNGAVAFGLYAPWKQSVHLIGSFNDWNQTADPLNISDRGIWWIIKEGLAAGEY
AYQFVIDGETIIGDPYARELRWAGGDQPQAIIHVGEPAYEWHDDGFGIRPLNELVIYELHVGDFSEAGTFKAVTERIPYL
QDLGVNALELMPLFEFPGDRSWGYNPAYFFAPESTYGTPADLRELIDTAHQHGIGVILDVVFNHVDHSSPINYLYPYDQS
PFFSSDGNPWGFPDLNHWNEAVKQLISDVQTYWLSDMHIDGFRYDHAEGIGFDGENGVSFLGWQARQIKPHVYLIAENLK
DYTTMVDQTDMDSSWHRSFHSQMFANLREGDFEGNQYGNVEATLGIIDFRNAGYNDNAQAINYLESHDEQRIVYEAQTNE
NISRETAYLKSRLGAIVLFTAAGVPMLYHGQEFGMDTERTIDKNMLKWELLQTAEGTDLHAFYRGMIQLRTSSKALVGNN
IRPVAMDAERKLLAYYRWADDGSEHVIVVVNFGITLQYLDVHFPVGGLWHEWVYNYDRETPEGMATIEVPGSGGKVFVLR

Sequences:

>Translated_560_residues
MQNDANPDASPTVASLPDGVWMGARDLGNGAVAFGLYAPWKQSVHLIGSFNDWNQTADPLNISDRGIWWIIKEGLAAGEY
AYQFVIDGETIIGDPYARELRWAGGDQPQAIIHVGEPAYEWHDDGFGIRPLNELVIYELHVGDFSEAGTFKAVTERIPYL
QDLGVNALELMPLFEFPGDRSWGYNPAYFFAPESTYGTPADLRELIDTAHQHGIGVILDVVFNHVDHSSPINYLYPYDQS
PFFSSDGNPWGFPDLNHWNEAVKQLISDVQTYWLSDMHIDGFRYDHAEGIGFDGENGVSFLGWQARQIKPHVYLIAENLK
DYTTMVDQTDMDSSWHRSFHSQMFANLREGDFEGNQYGNVEATLGIIDFRNAGYNDNAQAINYLESHDEQRIVYEAQTNE
NISRETAYLKSRLGAIVLFTAAGVPMLYHGQEFGMDTERTIDKNMLKWELLQTAEGTDLHAFYRGMIQLRTSSKALVGNN
IRPVAMDAERKLLAYYRWADDGSEHVIVVVNFGITLQYLDVHFPVGGLWHEWVYNYDRETPEGMATIEVPGSGGKVFVLR
>Mature_560_residues
MQNDANPDASPTVASLPDGVWMGARDLGNGAVAFGLYAPWKQSVHLIGSFNDWNQTADPLNISDRGIWWIIKEGLAAGEY
AYQFVIDGETIIGDPYARELRWAGGDQPQAIIHVGEPAYEWHDDGFGIRPLNELVIYELHVGDFSEAGTFKAVTERIPYL
QDLGVNALELMPLFEFPGDRSWGYNPAYFFAPESTYGTPADLRELIDTAHQHGIGVILDVVFNHVDHSSPINYLYPYDQS
PFFSSDGNPWGFPDLNHWNEAVKQLISDVQTYWLSDMHIDGFRYDHAEGIGFDGENGVSFLGWQARQIKPHVYLIAENLK
DYTTMVDQTDMDSSWHRSFHSQMFANLREGDFEGNQYGNVEATLGIIDFRNAGYNDNAQAINYLESHDEQRIVYEAQTNE
NISRETAYLKSRLGAIVLFTAAGVPMLYHGQEFGMDTERTIDKNMLKWELLQTAEGTDLHAFYRGMIQLRTSSKALVGNN
IRPVAMDAERKLLAYYRWADDGSEHVIVVVNFGITLQYLDVHFPVGGLWHEWVYNYDRETPEGMATIEVPGSGGKVFVLR

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=307, Percent_Identity=31.2703583061889, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI2367229, Length=542, Percent_Identity=25.0922509225092, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789839, Length=547, Percent_Identity=24.4972577696526, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786604, Length=400, Percent_Identity=25.5, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790687, Length=365, Percent_Identity=26.5753424657534, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554896, Length=277, Percent_Identity=29.6028880866426, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32564391, Length=170, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320826, Length=293, Percent_Identity=29.0102389078498, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321731, Length=99, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319776, Length=99, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28573410, Length=288, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 63285; Mature: 63285

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNDANPDASPTVASLPDGVWMGARDLGNGAVAFGLYAPWKQSVHLIGSFNDWNQTADPL
CCCCCCCCCCCCHHCCCCCCEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCC
NISDRGIWWIIKEGLAAGEYAYQFVIDGETIIGDPYARELRWAGGDQPQAIIHVGEPAYE
CCCCCCEEEEEECCCCCCCEEEEEEECCCEEECCCHHHHHCCCCCCCCCEEEEECCCCCE
WHDDGFGIRPLNELVIYELHVGDFSEAGTFKAVTERIPYLQDLGVNALELMPLFEFPGDR
ECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHCCCCCEEEEEHEECCCCC
SWGYNPAYFFAPESTYGTPADLRELIDTAHQHGIGVILDVVFNHVDHSSPINYLYPYDQS
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEECCCCC
PFFSSDGNPWGFPDLNHWNEAVKQLISDVQTYWLSDMHIDGFRYDHAEGIGFDGENGVSF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCEEECCCCCCCCCCCCCCEE
LGWQARQIKPHVYLIAENLKDYTTMVDQTDMDSSWHRSFHSQMFANLREGDFEGNQYGNV
ECCCHHCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
EATLGIIDFRNAGYNDNAQAINYLESHDEQRIVYEAQTNENISRETAYLKSRLGAIVLFT
EEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEE
AAGVPMLYHGQEFGMDTERTIDKNMLKWELLQTAEGTDLHAFYRGMIQLRTSSKALVGNN
CCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCEEECCC
IRPVAMDAERKLLAYYRWADDGSEHVIVVVNFGITLQYLDVHFPVGGLWHEWVYNYDRET
CCEEEECCCCEEEEEEEECCCCCCEEEEEEECCEEEEEEEEECCCCHHHHHHHHCCCCCC
PEGMATIEVPGSGGKVFVLR
CCCEEEEEECCCCCEEEEEC
>Mature Secondary Structure
MQNDANPDASPTVASLPDGVWMGARDLGNGAVAFGLYAPWKQSVHLIGSFNDWNQTADPL
CCCCCCCCCCCCHHCCCCCCEECCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCC
NISDRGIWWIIKEGLAAGEYAYQFVIDGETIIGDPYARELRWAGGDQPQAIIHVGEPAYE
CCCCCCEEEEEECCCCCCCEEEEEEECCCEEECCCHHHHHCCCCCCCCCEEEEECCCCCE
WHDDGFGIRPLNELVIYELHVGDFSEAGTFKAVTERIPYLQDLGVNALELMPLFEFPGDR
ECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHCCCCCEEEEEHEECCCCC
SWGYNPAYFFAPESTYGTPADLRELIDTAHQHGIGVILDVVFNHVDHSSPINYLYPYDQS
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEECCCCC
PFFSSDGNPWGFPDLNHWNEAVKQLISDVQTYWLSDMHIDGFRYDHAEGIGFDGENGVSF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCEEECCCCCCCCCCCCCCEE
LGWQARQIKPHVYLIAENLKDYTTMVDQTDMDSSWHRSFHSQMFANLREGDFEGNQYGNV
ECCCHHCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
EATLGIIDFRNAGYNDNAQAINYLESHDEQRIVYEAQTNENISRETAYLKSRLGAIVLFT
EEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEEE
AAGVPMLYHGQEFGMDTERTIDKNMLKWELLQTAEGTDLHAFYRGMIQLRTSSKALVGNN
CCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCEEECCC
IRPVAMDAERKLLAYYRWADDGSEHVIVVVNFGITLQYLDVHFPVGGLWHEWVYNYDRET
CCEEEECCCCEEEEEEEECCCCCCEEEEEEECCEEEEEEEEECCCCHHHHHHHHCCCCCC
PEGMATIEVPGSGGKVFVLR
CCCEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]