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The map label for this gene is nadR [H]

Identifier: 159897044

GI number: 159897044

Start: 596897

End: 597901

Strand: Reverse

Name: nadR [H]

Synonym: Haur_0514

Alternate gene names: 159897044

Gene position: 597901-596897 (Counterclockwise)

Preceding gene: 159897045

Following gene: 159897043

Centisome position: 9.42

GC content: 51.94

Gene sequence:

>1005_bases
ATGCGTGGTTTGACCCTCGGCAAATTTGCCCCCTTGCACAAAGGCCATCAACTGATGATTGAAACAGCGCTCAGCGAAGT
GGCTGAGTTGCAAATTATCATTTACGATAGCCCCGAGCAAACCAGCGTGCCATTGCCAGTTCGAAGCGCTTGGCTGCGTG
AGCTTTACCCCCAAGCCCAAGTGATTGAGGCGTGGGATGGGCCAAGCGTAATTGGCAATACCCGCGAGATTCAGCAACTC
CACGAAGATTATGTAATCCAGCAGCTTGGCATTAGCAATATCAGCCATTTTTATTCGAGCGAATTTTATGGTGAGCATAT
GAGCCAAGCGCTGGGCGCAATCAATCGCCAAGTTGATCCACCGCGCAACCAAGTGCCGATCTCGGCCACCCAAATTCGCA
GCGATCCCTATCGCTATCGTGAGTTTTTACATCCCCGAGTTTACCGCGATTTGATTATGAAAATCGTGCTGCTTGGTGCG
CCCTCAACTGGCAAAACCACCCTCACCGCCACGCTGGCCGAAACATTTAACACCCAATGGATGCCCGAATATGGCCGTGA
ATATTGGGAGCAACATCAGAGCAATCGGCGGCTCACGCCCCAGCAATTGGTCGAAATTGCCGAGGGCCATCGTGAGCGCG
AAGAACAGCGCCTGCTGGAAAGCAACCGTTATCTTTTTGTGGATACCAATGCCTTAACCACCTACCATTTTGCGCTGAGC
TATCATGGCAGGGCGGAGCCACGTTTGGCCCAACTGGCCGACCAAGCCCAACAGCGCTACGATTTGGTATTGGTTTGCGA
TAGCGATATTCCCTACGACGATACATGGGATCGCTCGGGCGAAGTTCAGCGCACGATCTTTCAAAAACAACTTTTCGCCC
AATTGCAGCAACGTCGCCAACCCTATTATTTAATTAGCGGCCCGCTTGAGCAGCGCATCGCCCAAGTGCAAAGCTTATTA
CAACGCCATCAGCTTTATCACAATCTGCTTGCCAACCCAGCCTGA

Upstream 100 bases:

>100_bases
ATAGCAACAAGAGCAATACAATCGTGCTGCATGTGCATCAATCAAACAATGATCAAACAATTGATAGCGAATTTACTCAG
CCTGAAGTTTAGGAACATCT

Downstream 100 bases:

>100_bases
GGTGTTTTATGGATCTTGCGAGTTTCTTCGATATTAATACGATTGCCTTTACGCTCTTTGGCTACCCCATGAGCTATCTC
GAATTAGCTGGTACGATCTT

Product: NAD metabolism ATPase/kinase

Products: NA

Alternate protein names: Transcriptional regulator NadR; Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRGLTLGKFAPLHKGHQLMIETALSEVAELQIIIYDSPEQTSVPLPVRSAWLRELYPQAQVIEAWDGPSVIGNTREIQQL
HEDYVIQQLGISNISHFYSSEFYGEHMSQALGAINRQVDPPRNQVPISATQIRSDPYRYREFLHPRVYRDLIMKIVLLGA
PSTGKTTLTATLAETFNTQWMPEYGREYWEQHQSNRRLTPQQLVEIAEGHREREEQRLLESNRYLFVDTNALTTYHFALS
YHGRAEPRLAQLADQAQQRYDLVLVCDSDIPYDDTWDRSGEVQRTIFQKQLFAQLQQRRQPYYLISGPLEQRIAQVQSLL
QRHQLYHNLLANPA

Sequences:

>Translated_334_residues
MRGLTLGKFAPLHKGHQLMIETALSEVAELQIIIYDSPEQTSVPLPVRSAWLRELYPQAQVIEAWDGPSVIGNTREIQQL
HEDYVIQQLGISNISHFYSSEFYGEHMSQALGAINRQVDPPRNQVPISATQIRSDPYRYREFLHPRVYRDLIMKIVLLGA
PSTGKTTLTATLAETFNTQWMPEYGREYWEQHQSNRRLTPQQLVEIAEGHREREEQRLLESNRYLFVDTNALTTYHFALS
YHGRAEPRLAQLADQAQQRYDLVLVCDSDIPYDDTWDRSGEVQRTIFQKQLFAQLQQRRQPYYLISGPLEQRIAQVQSLL
QRHQLYHNLLANPA
>Mature_334_residues
MRGLTLGKFAPLHKGHQLMIETALSEVAELQIIIYDSPEQTSVPLPVRSAWLRELYPQAQVIEAWDGPSVIGNTREIQQL
HEDYVIQQLGISNISHFYSSEFYGEHMSQALGAINRQVDPPRNQVPISATQIRSDPYRYREFLHPRVYRDLIMKIVLLGA
PSTGKTTLTATLAETFNTQWMPEYGREYWEQHQSNRRLTPQQLVEIAEGHREREEQRLLESNRYLFVDTNALTTYHFALS
YHGRAEPRLAQLADQAQQRYDLVLVCDSDIPYDDTWDRSGEVQRTIFQKQLFAQLQQRRQPYYLISGPLEQRIAQVQSLL
QRHQLYHNLLANPA

Specific function: This enzyme has three activities:DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase

COG id: COG3172

COG function: function code H; Predicted ATPase/kinase involved in NAD metabolism

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082440, Length=314, Percent_Identity=30.2547770700637, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016429
- InterPro:   IPR004821
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR006417
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: =2.7.7.1; =2.7.1.22 [H]

Molecular weight: Translated: 38856; Mature: 38856

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLTLGKFAPLHKGHQLMIETALSEVAELQIIIYDSPEQTSVPLPVRSAWLRELYPQAQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHCCCHH
VIEAWDGPSVIGNTREIQQLHEDYVIQQLGISNISHFYSSEFYGEHMSQALGAINRQVDP
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRNQVPISATQIRSDPYRYREFLHPRVYRDLIMKIVLLGAPSTGKTTLTATLAETFNTQW
CCCCCCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
MPEYGREYWEQHQSNRRLTPQQLVEIAEGHREREEQRLLESNRYLFVDTNALTTYHFALS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEE
YHGRAEPRLAQLADQAQQRYDLVLVCDSDIPYDDTWDRSGEVQRTIFQKQLFAQLQQRRQ
ECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PYYLISGPLEQRIAQVQSLLQRHQLYHNLLANPA
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGLTLGKFAPLHKGHQLMIETALSEVAELQIIIYDSPEQTSVPLPVRSAWLRELYPQAQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHCCCHH
VIEAWDGPSVIGNTREIQQLHEDYVIQQLGISNISHFYSSEFYGEHMSQALGAINRQVDP
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRNQVPISATQIRSDPYRYREFLHPRVYRDLIMKIVLLGAPSTGKTTLTATLAETFNTQW
CCCCCCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
MPEYGREYWEQHQSNRRLTPQQLVEIAEGHREREEQRLLESNRYLFVDTNALTTYHFALS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEE
YHGRAEPRLAQLADQAQQRYDLVLVCDSDIPYDDTWDRSGEVQRTIFQKQLFAQLQQRRQ
ECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PYYLISGPLEQRIAQVQSLLQRHQLYHNLLANPA
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 1327967 [H]