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The map label for this gene is lipB [H]

Identifier: 159896986

GI number: 159896986

Start: 531574

End: 532248

Strand: Reverse

Name: lipB [H]

Synonym: Haur_0454

Alternate gene names: 159896986

Gene position: 532248-531574 (Counterclockwise)

Preceding gene: 159896987

Following gene: 159896985

Centisome position: 8.39

GC content: 52.3

Gene sequence:

>675_bases
ATGACAATCCAGCAAACCCAACGTTTATTAAAGGTGCAAATCCTTGGGATGCTCGATTACAACGCCGCTTGGGAATTGCA
ACGCGAATTAGTCAGCCAACGCAGCGCTGAAACGATTCCCGATACCTTGGTATTGTTGGAGCATCCTGCCACTTACACGA
TTGGGCGCTCGGGCGGCTATGAGCATCTTTTGGTCAGCCCCGAAACCCTGCAACAACGTGGCGCAGCCTTGATCGAAGTT
GATCGCGGGGGCGATATTACCTACCATGGGCCAGGCCAGCTAGTCGGCTACCCCATTTTGCAAATTCGCGATCATGGCCG
CGATTTGCATCAATACTTGCGCATGCTCGAAGCAGTGATCATCGAACTGCTGGCTGAATATGGGCTAAGTGCGCGACGCT
TTCCTGGTTATACTGGCGTATGGGTGGGCGACCGCAAAATTGCCGCGATTGGGGTGCGCTCGAATACCCGTGGCGTTACG
TCGCATGGCTTTGCCTTGAATGTCACCACCAACTTGGCTGATTTTACCGCAATCGTGCCCTGTGGTATTCACGATTATGG
CGTAACCTCGTTGGCCGAATTATTGCCCGTTGTGCCAGCCTTCAACGATGTGCAACAACGGCTTATTCGAATTTTCCTAC
GCGTGTTTGATCTGCATTTGATCGAGCCTGTTTAG

Upstream 100 bases:

>100_bases
CTGATTATGTCGATTGGGCTGATAGCCAACCCGCCAGTTATGGCAGTCGCTTGACCCAAGCCTCACCCTTCGGCGGCGAG
ATGATTAGCTTGATGCCACT

Downstream 100 bases:

>100_bases
CCGAGGAACCGCAACCATGCCAACCATCCTCTCTGTTTGGGCGCACCCCGATGATGAAGCATTTGGCCCTGTTGGGGTGC
TGGCCCAAGCCCATGCTGCT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTIQQTQRLLKVQILGMLDYNAAWELQRELVSQRSAETIPDTLVLLEHPATYTIGRSGGYEHLLVSPETLQQRGAALIEV
DRGGDITYHGPGQLVGYPILQIRDHGRDLHQYLRMLEAVIIELLAEYGLSARRFPGYTGVWVGDRKIAAIGVRSNTRGVT
SHGFALNVTTNLADFTAIVPCGIHDYGVTSLAELLPVVPAFNDVQQRLIRIFLRVFDLHLIEPV

Sequences:

>Translated_224_residues
MTIQQTQRLLKVQILGMLDYNAAWELQRELVSQRSAETIPDTLVLLEHPATYTIGRSGGYEHLLVSPETLQQRGAALIEV
DRGGDITYHGPGQLVGYPILQIRDHGRDLHQYLRMLEAVIIELLAEYGLSARRFPGYTGVWVGDRKIAAIGVRSNTRGVT
SHGFALNVTTNLADFTAIVPCGIHDYGVTSLAELLPVVPAFNDVQQRLIRIFLRVFDLHLIEPV
>Mature_223_residues
TIQQTQRLLKVQILGMLDYNAAWELQRELVSQRSAETIPDTLVLLEHPATYTIGRSGGYEHLLVSPETLQQRGAALIEVD
RGGDITYHGPGQLVGYPILQIRDHGRDLHQYLRMLEAVIIELLAEYGLSARRFPGYTGVWVGDRKIAAIGVRSNTRGVTS
HGFALNVTTNLADFTAIVPCGIHDYGVTSLAELLPVVPAFNDVQQRLIRIFLRVFDLHLIEPV

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=222, Percent_Identity=40.5405405405405, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI87081767, Length=211, Percent_Identity=31.7535545023697, Blast_Score=110, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21355483, Length=219, Percent_Identity=38.3561643835616, Blast_Score=131, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24964; Mature: 24833

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQQTQRLLKVQILGMLDYNAAWELQRELVSQRSAETIPDTLVLLEHPATYTIGRSGGY
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCC
EHLLVSPETLQQRGAALIEVDRGGDITYHGPGQLVGYPILQIRDHGRDLHQYLRMLEAVI
CEEEECHHHHHHCCCEEEEEECCCCEEECCCCCEECCEEEEEECCCHHHHHHHHHHHHHH
IELLAEYGLSARRFPGYTGVWVGDRKIAAIGVRSNTRGVTSHGFALNVTTNLADFTAIVP
HHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCEEEEEECCHHHHHEEEC
CGIHDYGVTSLAELLPVVPAFNDVQQRLIRIFLRVFDLHLIEPV
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIQQTQRLLKVQILGMLDYNAAWELQRELVSQRSAETIPDTLVLLEHPATYTIGRSGGY
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCC
EHLLVSPETLQQRGAALIEVDRGGDITYHGPGQLVGYPILQIRDHGRDLHQYLRMLEAVI
CEEEECHHHHHHCCCEEEEEECCCCEEECCCCCEECCEEEEEECCCHHHHHHHHHHHHHH
IELLAEYGLSARRFPGYTGVWVGDRKIAAIGVRSNTRGVTSHGFALNVTTNLADFTAIVP
HHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCEEEEEECCHHHHHEEEC
CGIHDYGVTSLAELLPVVPAFNDVQQRLIRIFLRVFDLHLIEPV
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA