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The map label for this gene is pckG [H]

Identifier: 159896961

GI number: 159896961

Start: 500418

End: 502247

Strand: Reverse

Name: pckG [H]

Synonym: Haur_0429

Alternate gene names: 159896961

Gene position: 502247-500418 (Counterclockwise)

Preceding gene: 159896969

Following gene: 159896950

Centisome position: 7.91

GC content: 51.2

Gene sequence:

>1830_bases
ATGAACGCACCAGCCTATGTACGCAATCAAGAACTCAAACGTTGGGTCGAAGAAATGGTTGCCTTGTGCAAGCCCGACGA
TGTGTATTGGTGTGATGGTTCTGACGAAGAATATGCATTGATGTGTGGGCGCTTGGTTGAGTCTGGAACCTTCGTTCCAT
TGAATCCCGAATTACGCCCCAATAGCTATTTAGCCCGTTCAAATCCTTCCGACGTTGCTCGCGTCGAAGATCGCACGTTC
ATTTGTAGCTTGAGCAAAGGCGATGCAGGCCCAACCAATAACTGGATGCATCCCAAGGAAATGAAGGAAAAACTGAACGG
CTTGTTCGATGGCTCAATGCGTGGCCGCACCATGTACGTCGTACCATTCAGCATGGGGCCACTTGGCTCGCCAATCGCCC
ACATTGGGGTTGAATTGACCGACTCGCCCTATGTTGTGGTCAATATGAAGATTATGACCCGCATGAGCAGCGATGTCTAT
GCAGTGTTGGGCGAAACAGGTCACTTTATTCCTTGTTTGCACTCGGTTGGTGCACCACTGCAACCAGGCCAAGCCGATGT
TTCGTGGCCGTGTAACGAAAAAGAAAAATATATCGTGCACTTCCCCGAAGAACGTTCGATTTGGTCGTATGGTAGCGGGT
ATGGTGGCAATGCTTTGCTTGGCAAGAAATGCTTCGCCCTGCGGATTGCCTCGAATATGGCTCGCGACGAAGGTTGGTTG
GCTGAACATATGTTGGTGCTTGGGGTTGAATCGCCAGTTGGTGAAAAAACCTATGTGGCTGCTGCCTTCCCCAGTGCTTG
TGGCAAAACCAACTTTGCAATGCTGATTCCACCAACCGCAATGGATGGCTGGAAGGTAACTACGGTTGGCGATGACATTG
CTTGGATTAAACCTGGCAAAGATGGCAAATTGTATGCAATCAATCCAGAAAGCGGCTATTTTGGCGTAGCTCCAGGCACG
TCCGAGGACACCAATCCAAACGCAATGGCTGCCTTGAGCAAAAATACGATCTTTACCAACGTTGCGCTCACGCCTGAAGG
TGATATTTGGTGGGAAGGTATGACCAAAACCCCACCAGCCAAGGCAATCGACTGGACGGGCCAAGAATGGACTCCTGATT
GTGGCCGTAAAGCAGCTCACGCTAATGCCCGCTTTACCGCACCAGCCAGCCAATGTCCAAGCATCGACCCAGCTTGGGAA
GACCCCCAAGGCGTGCCGATTGAAGCCTTTATCTTTGGTGGTCGCCGTAGCACCTTGATGCCATTGGTCTACCAAGCCTT
CAACTGGACATATGGGGTCTATCAAGCAGCAACCATGGGATCGGAAACCACGGCAGCAGCAATGTCGGCAGTTGGCGAAG
TACGCCGCGACCCAATGGCAATGCTTCCATTCGTGGGCTACCATATGGCCGACTACTTCAACCACTGGTTGCAGTTTGGC
CGCACCATTCCAAACCCACCACGGATCTTCAGTGTCAACTGGTTCCGTAAAGACAAAAATGGCAAGTTCTTGTGGCCAGG
CTTCGGCGAAAATATGCGGGTGCTCAAATGGGTGGTCGAACGTGCTCACGGCCACGCAGTCGCCATCGAAAGCCCAATCG
GCTGGATGCCACGCTACCAAGACCTTGATTGGAGTGGCCTCAACTTCAGCGAAGACCAATTTCACGAGTTGATGTCGGTT
GATCGCGACGATTGGAATAAGGAAATTCTTTCGCACGAAGAATTGTTTATCAAACTTTACGACCGCTTGCCAAAAGAATT
TCTCTCGATTCGCGAGCTGATTCTTTCAAGCCTGTGGCGGTCACCCGAACACTGGGAAGCGTTCGAATAA

Upstream 100 bases:

>100_bases
AAGTTTAGCAGTGGGCTGGTATTCTATAGACAGGTTCGCGATGGCGTGCAGGGGGTTCACAGAAAACCCGACATCGCGAA
GCATTGGAGGAATCAGTATT

Downstream 100 bases:

>100_bases
GCGTTGGCTTGATCAGCCATAATCCTAATCGTCAAAGGCGGTTGCAAGCAAACTTGCAACCGCCTTTGTTATCAGCGAGA
CGAGAATACTACCAACGATT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MNAPAYVRNQELKRWVEEMVALCKPDDVYWCDGSDEEYALMCGRLVESGTFVPLNPELRPNSYLARSNPSDVARVEDRTF
ICSLSKGDAGPTNNWMHPKEMKEKLNGLFDGSMRGRTMYVVPFSMGPLGSPIAHIGVELTDSPYVVVNMKIMTRMSSDVY
AVLGETGHFIPCLHSVGAPLQPGQADVSWPCNEKEKYIVHFPEERSIWSYGSGYGGNALLGKKCFALRIASNMARDEGWL
AEHMLVLGVESPVGEKTYVAAAFPSACGKTNFAMLIPPTAMDGWKVTTVGDDIAWIKPGKDGKLYAINPESGYFGVAPGT
SEDTNPNAMAALSKNTIFTNVALTPEGDIWWEGMTKTPPAKAIDWTGQEWTPDCGRKAAHANARFTAPASQCPSIDPAWE
DPQGVPIEAFIFGGRRSTLMPLVYQAFNWTYGVYQAATMGSETTAAAMSAVGEVRRDPMAMLPFVGYHMADYFNHWLQFG
RTIPNPPRIFSVNWFRKDKNGKFLWPGFGENMRVLKWVVERAHGHAVAIESPIGWMPRYQDLDWSGLNFSEDQFHELMSV
DRDDWNKEILSHEELFIKLYDRLPKEFLSIRELILSSLWRSPEHWEAFE

Sequences:

>Translated_609_residues
MNAPAYVRNQELKRWVEEMVALCKPDDVYWCDGSDEEYALMCGRLVESGTFVPLNPELRPNSYLARSNPSDVARVEDRTF
ICSLSKGDAGPTNNWMHPKEMKEKLNGLFDGSMRGRTMYVVPFSMGPLGSPIAHIGVELTDSPYVVVNMKIMTRMSSDVY
AVLGETGHFIPCLHSVGAPLQPGQADVSWPCNEKEKYIVHFPEERSIWSYGSGYGGNALLGKKCFALRIASNMARDEGWL
AEHMLVLGVESPVGEKTYVAAAFPSACGKTNFAMLIPPTAMDGWKVTTVGDDIAWIKPGKDGKLYAINPESGYFGVAPGT
SEDTNPNAMAALSKNTIFTNVALTPEGDIWWEGMTKTPPAKAIDWTGQEWTPDCGRKAAHANARFTAPASQCPSIDPAWE
DPQGVPIEAFIFGGRRSTLMPLVYQAFNWTYGVYQAATMGSETTAAAMSAVGEVRRDPMAMLPFVGYHMADYFNHWLQFG
RTIPNPPRIFSVNWFRKDKNGKFLWPGFGENMRVLKWVVERAHGHAVAIESPIGWMPRYQDLDWSGLNFSEDQFHELMSV
DRDDWNKEILSHEELFIKLYDRLPKEFLSIRELILSSLWRSPEHWEAFE
>Mature_609_residues
MNAPAYVRNQELKRWVEEMVALCKPDDVYWCDGSDEEYALMCGRLVESGTFVPLNPELRPNSYLARSNPSDVARVEDRTF
ICSLSKGDAGPTNNWMHPKEMKEKLNGLFDGSMRGRTMYVVPFSMGPLGSPIAHIGVELTDSPYVVVNMKIMTRMSSDVY
AVLGETGHFIPCLHSVGAPLQPGQADVSWPCNEKEKYIVHFPEERSIWSYGSGYGGNALLGKKCFALRIASNMARDEGWL
AEHMLVLGVESPVGEKTYVAAAFPSACGKTNFAMLIPPTAMDGWKVTTVGDDIAWIKPGKDGKLYAINPESGYFGVAPGT
SEDTNPNAMAALSKNTIFTNVALTPEGDIWWEGMTKTPPAKAIDWTGQEWTPDCGRKAAHANARFTAPASQCPSIDPAWE
DPQGVPIEAFIFGGRRSTLMPLVYQAFNWTYGVYQAATMGSETTAAAMSAVGEVRRDPMAMLPFVGYHMADYFNHWLQFG
RTIPNPPRIFSVNWFRKDKNGKFLWPGFGENMRVLKWVVERAHGHAVAIESPIGWMPRYQDLDWSGLNFSEDQFHELMSV
DRDDWNKEILSHEELFIKLYDRLPKEFLSIRELILSSLWRSPEHWEAFE

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=597, Percent_Identity=51.2562814070352, Blast_Score=592, Evalue=1e-169,
Organism=Homo sapiens, GI187281517, Length=603, Percent_Identity=49.4195688225539, Blast_Score=583, Evalue=1e-166,
Organism=Homo sapiens, GI66346723, Length=377, Percent_Identity=53.315649867374, Blast_Score=391, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71989645, Length=572, Percent_Identity=49.6503496503497, Blast_Score=569, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989658, Length=572, Percent_Identity=49.6503496503497, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17555492, Length=583, Percent_Identity=49.2281303602058, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989653, Length=559, Percent_Identity=50.2683363148479, Blast_Score=560, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI32564411, Length=554, Percent_Identity=50.1805054151625, Blast_Score=549, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=504, Percent_Identity=50.9920634920635, Blast_Score=517, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71989649, Length=482, Percent_Identity=50.8298755186722, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI32564409, Length=337, Percent_Identity=50.4451038575668, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17540956, Length=525, Percent_Identity=26.0952380952381, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24655085, Length=569, Percent_Identity=52.5483304042179, Blast_Score=596, Evalue=1e-170,
Organism=Drosophila melanogaster, GI24655082, Length=585, Percent_Identity=50.9401709401709, Blast_Score=583, Evalue=1e-167,
Organism=Drosophila melanogaster, GI161077226, Length=545, Percent_Identity=51.9266055045872, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24655088, Length=471, Percent_Identity=54.140127388535, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68265; Mature: 68265

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPAYVRNQELKRWVEEMVALCKPDDVYWCDGSDEEYALMCGRLVESGTFVPLNPELRP
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCC
NSYLARSNPSDVARVEDRTFICSLSKGDAGPTNNWMHPKEMKEKLNGLFDGSMRGRTMYV
CCEECCCCCCHHHEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
VPFSMGPLGSPIAHIGVELTDSPYVVVNMKIMTRMSSDVYAVLGETGHFIPCLHSVGAPL
EEECCCCCCCCHHHCCEEECCCCEEEEEEEEEHHCCCCEEEEECCCCCHHHHHHHCCCCC
QPGQADVSWPCNEKEKYIVHFPEERSIWSYGSGYGGNALLGKKCFALRIASNMARDEGWL
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHCCCCCHH
AEHMLVLGVESPVGEKTYVAAAFPSACGKTNFAMLIPPTAMDGWKVTTVGDDIAWIKPGK
HHEEEEEECCCCCCCCEEEEEECCHHCCCCCEEEEECCCCCCCEEEEEECCCEEEECCCC
DGKLYAINPESGYFGVAPGTSEDTNPNAMAALSKNTIFTNVALTPEGDIWWEGMTKTPPA
CCEEEEECCCCCEEECCCCCCCCCCCCCEEEEECCEEEEEEEECCCCCEEECCCCCCCCC
KAIDWTGQEWTPDCGRKAAHANARFTAPASQCPSIDPAWEDPQGVPIEAFIFGGRRSTLM
CEEECCCCCCCCCCCCHHHCCCCEECCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHH
PLVYQAFNWTYGVYQAATMGSETTAAAMSAVGEVRRDPMAMLPFVGYHMADYFNHWLQFG
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
RTIPNPPRIFSVNWFRKDKNGKFLWPGFGENMRVLKWVVERAHGHAVAIESPIGWMPRYQ
CCCCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DLDWSGLNFSEDQFHELMSVDRDDWNKEILSHEELFIKLYDRLPKEFLSIRELILSSLWR
CCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPEHWEAFE
CCCCCCCCC
>Mature Secondary Structure
MNAPAYVRNQELKRWVEEMVALCKPDDVYWCDGSDEEYALMCGRLVESGTFVPLNPELRP
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCC
NSYLARSNPSDVARVEDRTFICSLSKGDAGPTNNWMHPKEMKEKLNGLFDGSMRGRTMYV
CCEECCCCCCHHHEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEE
VPFSMGPLGSPIAHIGVELTDSPYVVVNMKIMTRMSSDVYAVLGETGHFIPCLHSVGAPL
EEECCCCCCCCHHHCCEEECCCCEEEEEEEEEHHCCCCEEEEECCCCCHHHHHHHCCCCC
QPGQADVSWPCNEKEKYIVHFPEERSIWSYGSGYGGNALLGKKCFALRIASNMARDEGWL
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHCCCCCHH
AEHMLVLGVESPVGEKTYVAAAFPSACGKTNFAMLIPPTAMDGWKVTTVGDDIAWIKPGK
HHEEEEEECCCCCCCCEEEEEECCHHCCCCCEEEEECCCCCCCEEEEEECCCEEEECCCC
DGKLYAINPESGYFGVAPGTSEDTNPNAMAALSKNTIFTNVALTPEGDIWWEGMTKTPPA
CCEEEEECCCCCEEECCCCCCCCCCCCCEEEEECCEEEEEEEECCCCCEEECCCCCCCCC
KAIDWTGQEWTPDCGRKAAHANARFTAPASQCPSIDPAWEDPQGVPIEAFIFGGRRSTLM
CEEECCCCCCCCCCCCHHHCCCCEECCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHH
PLVYQAFNWTYGVYQAATMGSETTAAAMSAVGEVRRDPMAMLPFVGYHMADYFNHWLQFG
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
RTIPNPPRIFSVNWFRKDKNGKFLWPGFGENMRVLKWVVERAHGHAVAIESPIGWMPRYQ
CCCCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DLDWSGLNFSEDQFHELMSVDRDDWNKEILSHEELFIKLYDRLPKEFLSIRELILSSLWR
CCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPEHWEAFE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA