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The map label for this gene is ytcB [H]

Identifier: 159896837

GI number: 159896837

Start: 362993

End: 363946

Strand: Direct

Name: ytcB [H]

Synonym: Haur_0304

Alternate gene names: 159896837

Gene position: 362993-363946 (Clockwise)

Preceding gene: 159896836

Following gene: 159896838

Centisome position: 5.72

GC content: 50.52

Gene sequence:

>954_bases
GTGTTGGTTTTGATCACAGGTTCCAGTGGGCAGATTGGCACCAATTTGGCGCTACGCCTTCTCGCAGATGGCCATGAAGT
GTTTGGGGTTGATCAACGGGTCAACACGTGGACAAAAGCCTTTGAGTATGTGATTCAGGATCTTGGCGCACCCTACCGCG
ATTTCCAAGGCGGAATCGGCGGCGTGCCCTATCCTAAGCCTGATGTTGTGGTGCATTTGGCGGCCAATGCCAAAGTCCAC
GAACTAGTAACCTACCCCCATCGAGCGTTGGAAAATGTGATGACCACCTACAACGTATTGGAATACTGTCGCCATCAACA
AGTTCCAATCATTTTTAGCTCATCACGCGAAGTCTATGGTGATATTCATCGCTACTTAACCGAAGAAGCCCAAGCCGATT
TCGTCTATACCGAAAGCCCCTATTCGGCCTCAAAAATCAGCGGCGAAGCATTTATCTATTCCTATGCCCGATGTTACAAC
TTGCCCTATTTGGTTTTTCGTTTCTCGAATGTCTATGGGCGCTACGATAACGACATCGAACGCATGGAGCGGGTAATTCC
CTTGTTCATTCGCCGCATGCGCGACGGCCAACCTGTGACCGTCTTTGGGCGTGAAAAAACCCTCGATTTCACCTATGTCG
ATGATTGTGTCGATGGGATTGTGCGCGGAATCGAGCGAATTGTCAGTGGCCAAGTTGCCAATCAGACCTTTAATTTAGCC
TATGGTGAAGGCAATACCTTGGTTCGTATGGCCGAATTGATCGCCGAGGCTCTGCAAATTGAAGCAACGATTAACGTGCA
ACCGTCAAAACTCGCTGGTGAAGTAACCTACTATGTGGCCAACATCGGGCGAGCACGCCAACTCTTGGGTTACACGCCCC
AAGTTGCTTTAGCCGATGGTTTACGGCGAGCAGTCGCGTGGAACATTGCGTGGGACGAGGAACAGCGGCGATGA

Upstream 100 bases:

>100_bases
CTGCCAGCCCATGTACGGCGTGCAGTGGGCTGGCACATTCCACATAGCCAAATCCGCATGCTATAATCGCAGCCACAACA
TACTAATCAAGGAGTCTCCC

Downstream 100 bases:

>100_bases
CTGAACGATCACGGCAACGCGGCCATCAACAGCTCCAAACTATGCGCAGCAAACGGATTCAGGCGCGACTGCTAGCGCTC
TCACGGGTCATGAACGATAT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MLVLITGSSGQIGTNLALRLLADGHEVFGVDQRVNTWTKAFEYVIQDLGAPYRDFQGGIGGVPYPKPDVVVHLAANAKVH
ELVTYPHRALENVMTTYNVLEYCRHQQVPIIFSSSREVYGDIHRYLTEEAQADFVYTESPYSASKISGEAFIYSYARCYN
LPYLVFRFSNVYGRYDNDIERMERVIPLFIRRMRDGQPVTVFGREKTLDFTYVDDCVDGIVRGIERIVSGQVANQTFNLA
YGEGNTLVRMAELIAEALQIEATINVQPSKLAGEVTYYVANIGRARQLLGYTPQVALADGLRRAVAWNIAWDEEQRR

Sequences:

>Translated_317_residues
MLVLITGSSGQIGTNLALRLLADGHEVFGVDQRVNTWTKAFEYVIQDLGAPYRDFQGGIGGVPYPKPDVVVHLAANAKVH
ELVTYPHRALENVMTTYNVLEYCRHQQVPIIFSSSREVYGDIHRYLTEEAQADFVYTESPYSASKISGEAFIYSYARCYN
LPYLVFRFSNVYGRYDNDIERMERVIPLFIRRMRDGQPVTVFGREKTLDFTYVDDCVDGIVRGIERIVSGQVANQTFNLA
YGEGNTLVRMAELIAEALQIEATINVQPSKLAGEVTYYVANIGRARQLLGYTPQVALADGLRRAVAWNIAWDEEQRR
>Mature_317_residues
MLVLITGSSGQIGTNLALRLLADGHEVFGVDQRVNTWTKAFEYVIQDLGAPYRDFQGGIGGVPYPKPDVVVHLAANAKVH
ELVTYPHRALENVMTTYNVLEYCRHQQVPIIFSSSREVYGDIHRYLTEEAQADFVYTESPYSASKISGEAFIYSYARCYN
LPYLVFRFSNVYGRYDNDIERMERVIPLFIRRMRDGQPVTVFGREKTLDFTYVDDCVDGIVRGIERIVSGQVANQTFNLA
YGEGNTLVRMAELIAEALQIEATINVQPSKLAGEVTYYVANIGRARQLLGYTPQVALADGLRRAVAWNIAWDEEQRR

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=28.6604361370716, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI7657641, Length=325, Percent_Identity=24, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=27.0422535211268, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994969, Length=280, Percent_Identity=27.8571428571429, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786974, Length=316, Percent_Identity=25.6329113924051, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=26.2839879154079, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI115532424, Length=251, Percent_Identity=27.4900398406374, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=333, Percent_Identity=25.5255255255255, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=321, Percent_Identity=28.6604361370716, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 35873; Mature: 35873

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLITGSSGQIGTNLALRLLADGHEVFGVDQRVNTWTKAFEYVIQDLGAPYRDFQGGIG
CEEEEECCCCCCCHHHHEEEEECCCHHEECCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
GVPYPKPDVVVHLAANAKVHELVTYPHRALENVMTTYNVLEYCRHQQVPIIFSSSREVYG
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHH
DIHRYLTEEAQADFVYTESPYSASKISGEAFIYSYARCYNLPYLVFRFSNVYGRYDNDIE
HHHHHHHHHHCCCEEEECCCCCHHHCCCHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHH
RMERVIPLFIRRMRDGQPVTVFGREKTLDFTYVDDCVDGIVRGIERIVSGQVANQTFNLA
HHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHHCCHHCCCEEEEE
YGEGNTLVRMAELIAEALQIEATINVQPSKLAGEVTYYVANIGRARQLLGYTPQVALADG
ECCCCHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LRRAVAWNIAWDEEQRR
HHHHHEEEEECCHHHCC
>Mature Secondary Structure
MLVLITGSSGQIGTNLALRLLADGHEVFGVDQRVNTWTKAFEYVIQDLGAPYRDFQGGIG
CEEEEECCCCCCCHHHHEEEEECCCHHEECCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
GVPYPKPDVVVHLAANAKVHELVTYPHRALENVMTTYNVLEYCRHQQVPIIFSSSREVYG
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHH
DIHRYLTEEAQADFVYTESPYSASKISGEAFIYSYARCYNLPYLVFRFSNVYGRYDNDIE
HHHHHHHHHHCCCEEEECCCCCHHHCCCHHHHHHHHHHHCCCCEEEEHHHHCCCCCCHHH
RMERVIPLFIRRMRDGQPVTVFGREKTLDFTYVDDCVDGIVRGIERIVSGQVANQTFNLA
HHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHHCCHHCCCEEEEE
YGEGNTLVRMAELIAEALQIEATINVQPSKLAGEVTYYVANIGRARQLLGYTPQVALADG
ECCCCHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LRRAVAWNIAWDEEQRR
HHHHHEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]