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The map label for this gene is hemK [H]

Identifier: 159896800

GI number: 159896800

Start: 315134

End: 315985

Strand: Direct

Name: hemK [H]

Synonym: Haur_0267

Alternate gene names: 159896800

Gene position: 315134-315985 (Clockwise)

Preceding gene: 159896798

Following gene: 159896802

Centisome position: 4.97

GC content: 53.87

Gene sequence:

>852_bases
ATGGCTATAACAACTGTGCGCGAGGCATTAATTGAGGCAGCTAGCTCTTTGATCAGCGCTTCGCTGACCCCGCAACTCGA
TGCCCGCGTGCTGCTGGGCCATGTGCTTGGTCTTACGCAGACCCAAATCTTGGCGGCCTTCAACGATCAGCTCTCGACTG
AGCAGGTTGAGCAATTTCAGGGCTTAATTGAACGGCGTAGCGCCTTAGAACCAATCGCCTATCTGATTGGCTCGCGTGAG
TTTTATGGCTTAATGTTTAACGTTGATCGGCGGGTTTTGGTGCCACGGCCTGATACTGAGATTTTGGTGGAGCAGGCATT
AACCTGGATCAAACAGCAGCAACGCCCGTTGGTGGTGGCCGATATTGGCACGGGCAGCGGTTGTATTGCGGTGGCGGTGA
CCAAACATGCACCCACGATTAAAATGTATGCAGTTGATCTTTCGCCCGCTGCCTTGGCCGTGGCTCAAAGTAATGTGGAG
CGCCATGGCTTGCAGCAACAGATTGAGCTGATTCATGGCGATGGTGTCAGTCAATTGCCAGAGCCAATTGATTTGCTTTT
GAGCAACCCACCCTATACCTTGCTCGACGAAATTGAGCCTGGAGTACGGTTGCACGAGCCAACCCTCGCACTCGATGGTG
GCCCCGATGGGCTTGATTGCTATCGCCAATTATTGCCAGCAACGGCGGCAATTTTGCGCCAAGGCCTGCCGAGCGCAGCG
CTGTTTGAGATTGGGGCTTGGCAAGGGTCAGAGGTTATTGCCCTTGCGCAAGCCAGTTTTCCGCAGGCCAAGATTCAACT
TGTACGCGATTTAGCTGCCCGTGATCGGGTGGTGCAAATTGAGCAGGTCTAG

Upstream 100 bases:

>100_bases
TGCTGCTAGCACAATGATGCCTGATTCGCCTAGGAGTCTCTAGATGTGCAGATTACGAGTGCATTACGTAGGTATCGCAA
TATGCTGAGGAATTTCTGAG

Downstream 100 bases:

>100_bases
CGTGCTCCAAAGTTGACTGTCCAATAAATTTGGTATTCTGAATTGGGATTACGGGCGATCCCAACCCCAATTTCGCTGAC
ATCGGCATATAGAATGTTGG

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; M.RprHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAITTVREALIEAASSLISASLTPQLDARVLLGHVLGLTQTQILAAFNDQLSTEQVEQFQGLIERRSALEPIAYLIGSRE
FYGLMFNVDRRVLVPRPDTEILVEQALTWIKQQQRPLVVADIGTGSGCIAVAVTKHAPTIKMYAVDLSPAALAVAQSNVE
RHGLQQQIELIHGDGVSQLPEPIDLLLSNPPYTLLDEIEPGVRLHEPTLALDGGPDGLDCYRQLLPATAAILRQGLPSAA
LFEIGAWQGSEVIALAQASFPQAKIQLVRDLAARDRVVQIEQV

Sequences:

>Translated_283_residues
MAITTVREALIEAASSLISASLTPQLDARVLLGHVLGLTQTQILAAFNDQLSTEQVEQFQGLIERRSALEPIAYLIGSRE
FYGLMFNVDRRVLVPRPDTEILVEQALTWIKQQQRPLVVADIGTGSGCIAVAVTKHAPTIKMYAVDLSPAALAVAQSNVE
RHGLQQQIELIHGDGVSQLPEPIDLLLSNPPYTLLDEIEPGVRLHEPTLALDGGPDGLDCYRQLLPATAAILRQGLPSAA
LFEIGAWQGSEVIALAQASFPQAKIQLVRDLAARDRVVQIEQV
>Mature_282_residues
AITTVREALIEAASSLISASLTPQLDARVLLGHVLGLTQTQILAAFNDQLSTEQVEQFQGLIERRSALEPIAYLIGSREF
YGLMFNVDRRVLVPRPDTEILVEQALTWIKQQQRPLVVADIGTGSGCIAVAVTKHAPTIKMYAVDLSPAALAVAQSNVER
HGLQQQIELIHGDGVSQLPEPIDLLLSNPPYTLLDEIEPGVRLHEPTLALDGGPDGLDCYRQLLPATAAILRQGLPSAAL
FEIGAWQGSEVIALAQASFPQAKIQLVRDLAARDRVVQIEQV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=278, Percent_Identity=30.9352517985612, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787463, Length=274, Percent_Identity=36.4963503649635, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082085, Length=184, Percent_Identity=38.5869565217391, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324266, Length=181, Percent_Identity=28.7292817679558, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582226, Length=274, Percent_Identity=27.007299270073, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 30683; Mature: 30552

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITTVREALIEAASSLISASLTPQLDARVLLGHVLGLTQTQILAAFNDQLSTEQVEQFQ
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GLIERRSALEPIAYLIGSREFYGLMFNVDRRVLVPRPDTEILVEQALTWIKQQQRPLVVA
HHHHHHHHHHHHHHHHCCHHHEEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
DIGTGSGCIAVAVTKHAPTIKMYAVDLSPAALAVAQSNVERHGLQQQIELIHGDGVSQLP
ECCCCCCEEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EPIDLLLSNPPYTLLDEIEPGVRLHEPTLALDGGPDGLDCYRQLLPATAAILRQGLPSAA
CCHHHHCCCCCCHHHHCCCCCCEEECCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHH
LFEIGAWQGSEVIALAQASFPQAKIQLVRDLAARDRVVQIEQV
HHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
AITTVREALIEAASSLISASLTPQLDARVLLGHVLGLTQTQILAAFNDQLSTEQVEQFQ
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GLIERRSALEPIAYLIGSREFYGLMFNVDRRVLVPRPDTEILVEQALTWIKQQQRPLVVA
HHHHHHHHHHHHHHHHCCHHHEEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
DIGTGSGCIAVAVTKHAPTIKMYAVDLSPAALAVAQSNVERHGLQQQIELIHGDGVSQLP
ECCCCCCEEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EPIDLLLSNPPYTLLDEIEPGVRLHEPTLALDGGPDGLDCYRQLLPATAAILRQGLPSAA
CCHHHHCCCCCCHHHHCCCCCCEEECCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHH
LFEIGAWQGSEVIALAQASFPQAKIQLVRDLAARDRVVQIEQV
HHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]