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The map label for this gene is thiB [H]

Identifier: 159896794

GI number: 159896794

Start: 306391

End: 307551

Strand: Direct

Name: thiB [H]

Synonym: Haur_0261

Alternate gene names: 159896794

Gene position: 306391-307551 (Clockwise)

Preceding gene: 159896789

Following gene: 159896796

Centisome position: 4.83

GC content: 52.63

Gene sequence:

>1161_bases
GTGAAACGTTGGTTACTGAGTTTTTTAACAATTGGTTCGTTGGTTTTGACTGGCTGTGGTGCGGCGGCTACGCCAACCGC
TGAAACCGCGATTCAGCCTACTCCGGCCACCACGGCTGCCGCTGATGTTACGCCTGCAGCAACCCAAGCCACCCAAGCGC
CGACCGATAGCGGCCAGTTTGCCGGCCAAACCTTGGTGGTGGTTTCGCACGATAGTTTTGCAATCAGCAGCGAAGTTATC
TCAGGCTTTGAGCAAATGACTGGCGCGACCGTCCAAATTCTAGAATCGGGCGATGCTGGCGAGGCGCTCAATAAGAGCAT
TTTGGCCAAAGGCGCACCCTTGGGCGATGTGTTGTATGGGGTCGATAATACCTTTTTGAGTCGGGCGCTTGATGCTGATA
TTTTCGAGGCCTACGCTGCCAAGAATCTCGAGCAAATTCCTGCTGAATTGAAGCTCGATAGCCAAAATCGGGTTTCGCCC
GTTGATGTTGGCTATGTCACGATCAATTACGATAAAGCTGCCTTGGCCGAGGCTGGCCTGAGCTTGCCAACCGATTTGCG
CGATTTAACCAAGCCTGAGTGGAAAGGCAAGTTGGTGGTTGAAAATCCGGCAACGTCATCGCCTGGCTTATCATTTATGT
TGGCGACGATTGCCCACTTTGGCGAGAGCAGCGATTATACCTGGCGCAACTTCTGGAGCGACCTGCGAGCCAATGAGATC
AAAGTGGCTAGCGGTTGGGAAGAAGCCTACTATGGCGATTTTAGCGGAGCCAGTGATGGTCAATATCCGTTGGTCGTCAG
CTATGCGACCAGCCCTGCTGCCGAAGTTATCTTTGCGACAACGCCCTTGACCGATGCACCGACTGGCAATTTGTTGTTAC
CTGGTGGCGCATTCCAGCAAATTGAATTTGTGGGCTTGCTAAAAGATGCCAAAAACCCTGAATTGGGCAAAGCTTGGATT
GACTATATGCTGAGCGACACCTTCCAGAGCGATATTGGCGGACAGATGTTTGTGTACCCAGCCCTGCCTAGCGCTAAAGT
GCCAGCTGAATTTACTCAATATGCCCAAGTGCCTAGCAATGTAACGACGCTTGCGCCAGCCGAGATTGCTGCCAACCGCG
AGCGCTGGCTCAACGAATGGCAAGAAACCGTCCTACGCTAA

Upstream 100 bases:

>100_bases
GATCTCGTTAGCACGAGCGAAGGAAAGCGGCGTGGTTTGATCCCGCCGTGCGCGGGATTTTGCATGCTGGCCCCTAGCCA
ATGTTCAAGGAGTTTATTTC

Downstream 100 bases:

>100_bases
ACCAAAACATCCCCTGCAACTTTAATTAAGTGTTGCAGGGGATGTTTATGAGCAAAAATGCAGTTTAGTCGCCAGTGGTT
TTGACTGCTGGCCAATCGCC

Product: ABC transporter periplasmic-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKRWLLSFLTIGSLVLTGCGAAATPTAETAIQPTPATTAAADVTPAATQATQAPTDSGQFAGQTLVVVSHDSFAISSEVI
SGFEQMTGATVQILESGDAGEALNKSILAKGAPLGDVLYGVDNTFLSRALDADIFEAYAAKNLEQIPAELKLDSQNRVSP
VDVGYVTINYDKAALAEAGLSLPTDLRDLTKPEWKGKLVVENPATSSPGLSFMLATIAHFGESSDYTWRNFWSDLRANEI
KVASGWEEAYYGDFSGASDGQYPLVVSYATSPAAEVIFATTPLTDAPTGNLLLPGGAFQQIEFVGLLKDAKNPELGKAWI
DYMLSDTFQSDIGGQMFVYPALPSAKVPAEFTQYAQVPSNVTTLAPAEIAANRERWLNEWQETVLR

Sequences:

>Translated_386_residues
MKRWLLSFLTIGSLVLTGCGAAATPTAETAIQPTPATTAAADVTPAATQATQAPTDSGQFAGQTLVVVSHDSFAISSEVI
SGFEQMTGATVQILESGDAGEALNKSILAKGAPLGDVLYGVDNTFLSRALDADIFEAYAAKNLEQIPAELKLDSQNRVSP
VDVGYVTINYDKAALAEAGLSLPTDLRDLTKPEWKGKLVVENPATSSPGLSFMLATIAHFGESSDYTWRNFWSDLRANEI
KVASGWEEAYYGDFSGASDGQYPLVVSYATSPAAEVIFATTPLTDAPTGNLLLPGGAFQQIEFVGLLKDAKNPELGKAWI
DYMLSDTFQSDIGGQMFVYPALPSAKVPAEFTQYAQVPSNVTTLAPAEIAANRERWLNEWQETVLR
>Mature_386_residues
MKRWLLSFLTIGSLVLTGCGAAATPTAETAIQPTPATTAAADVTPAATQATQAPTDSGQFAGQTLVVVSHDSFAISSEVI
SGFEQMTGATVQILESGDAGEALNKSILAKGAPLGDVLYGVDNTFLSRALDADIFEAYAAKNLEQIPAELKLDSQNRVSP
VDVGYVTINYDKAALAEAGLSLPTDLRDLTKPEWKGKLVVENPATSSPGLSFMLATIAHFGESSDYTWRNFWSDLRANEI
KVASGWEEAYYGDFSGASDGQYPLVVSYATSPAAEVIFATTPLTDAPTGNLLLPGGAFQQIEFVGLLKDAKNPELGKAWI
DYMLSDTFQSDIGGQMFVYPALPSAKVPAEFTQYAQVPSNVTTLAPAEIAANRERWLNEWQETVLR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=332, Percent_Identity=28.3132530120482, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41165; Mature: 41165

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWLLSFLTIGSLVLTGCGAAATPTAETAIQPTPATTAAADVTPAATQATQAPTDSGQF
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
AGQTLVVVSHDSFAISSEVISGFEQMTGATVQILESGDAGEALNKSILAKGAPLGDVLYG
CCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHC
VDNTFLSRALDADIFEAYAAKNLEQIPAELKLDSQNRVSPVDVGYVTINYDKAALAEAGL
CCHHHHHHHHHHHHHHHHHHCCHHHCCHHEEECCCCCCCCEEEEEEEEECCHHHHHHCCC
SLPTDLRDLTKPEWKGKLVVENPATSSPGLSFMLATIAHFGESSDYTWRNFWSDLRANEI
CCCCCHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEE
KVASGWEEAYYGDFSGASDGQYPLVVSYATSPAAEVIFATTPLTDAPTGNLLLPGGAFQQ
EEECCCCHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEECCCCHHH
IEFVGLLKDAKNPELGKAWIDYMLSDTFQSDIGGQMFVYPALPSAKVPAEFTQYAQVPSN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCC
VTTLAPAEIAANRERWLNEWQETVLR
CEECCCHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRWLLSFLTIGSLVLTGCGAAATPTAETAIQPTPATTAAADVTPAATQATQAPTDSGQF
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
AGQTLVVVSHDSFAISSEVISGFEQMTGATVQILESGDAGEALNKSILAKGAPLGDVLYG
CCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHC
VDNTFLSRALDADIFEAYAAKNLEQIPAELKLDSQNRVSPVDVGYVTINYDKAALAEAGL
CCHHHHHHHHHHHHHHHHHHCCHHHCCHHEEECCCCCCCCEEEEEEEEECCHHHHHHCCC
SLPTDLRDLTKPEWKGKLVVENPATSSPGLSFMLATIAHFGESSDYTWRNFWSDLRANEI
CCCCCHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEE
KVASGWEEAYYGDFSGASDGQYPLVVSYATSPAAEVIFATTPLTDAPTGNLLLPGGAFQQ
EEECCCCHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEECCCCHHH
IEFVGLLKDAKNPELGKAWIDYMLSDTFQSDIGGQMFVYPALPSAKVPAEFTQYAQVPSN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCC
VTTLAPAEIAANRERWLNEWQETVLR
CEECCCHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]