Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 159896679

GI number: 159896679

Start: 177260

End: 178546

Strand: Reverse

Name: eno [H]

Synonym: Haur_0146

Alternate gene names: 159896679

Gene position: 178546-177260 (Counterclockwise)

Preceding gene: 159896680

Following gene: 159896678

Centisome position: 2.81

GC content: 52.06

Gene sequence:

>1287_bases
ATGGCACTCATTACGCACGTTCGCGGTCGCGAAGTTCTCGATAGTCGAGGTAACCCGACCGTTGAAGTTGAAATCGGTTT
GGAAGATGGCACGCTTGCCCGCGCAATTGTTCCTTCGGGCGCTTCAACTGGCGCACACGAAGCAGTCGAACTGCGCGACG
GCGACAAAAGCCGCTACGGCGGTAAAGGCGTGCTCAAAGCTGTAAAGCATGTCAATGAAGATATTGCTGAAGCCATCGAA
GGCTTAGAGGCACTTGATCAAATTACGCTTGACAAGACCTTGATTGAGCTTGATGGCACTAAAAATAAAGGTAAACTTGG
CGCTAATGCAATCTTAGGGGTTTCATTGGCCACTGCCAAAGCTGGCGCATTGTCAGTTGGTTTGCCGCTCTATCGCTACC
TTGGCGGGGTTTTCGGTCATACCTTGCCAGTCCCAATGATGAACATCTTGAATGGTGGCAAGCACGCCCAAGATAGCACC
GACTTCCAAGAATTTATGGTGATGCCAGTCGGCGCTCCATCGTTCCGCGAAGCTCTACGCTGGGGTGCAGAAATTTACCA
CACGCTCAAGAATGTGTTGCACGACCGTGGCTTGGCCACCAATACTGGCGATGAAGGTGGTTTTGCGCCATCGTTGCCAT
CAAATGAAGCCGCTCTCGAAGTAATCGTCGAAGCGATCCAAAAGGCTGGCTACACCCCAGGCAAAGATGTCATGTTAGCG
CTCGACCCAGCATGTAGCGAAATCTACGAAAATGGCATCTACAACTTGGCCCGCGAAGGCCGCAAACTTAGCTCCGAAGA
AATGGTGGGCTACTGGGAAAATCTGGTCAACAAATACCCAATCATTTCAATCGAAGACGGTTTGCACGAAGACGATTGGG
GTGCTTGGTCGTCGTTGCGCCAACGTATCGGCGATCGTGTGCAGTTGGTCGGCGACGATTTGTTGGTAACCAACGTCGAA
CGGCTCAACCGTGCGATCAAAGAAAACTCAGCGAACAGTATTTTGATCAAGCTTAACCAAATTGGCACCTTGACCGAAAC
CCTCGACGCAATTCAAATGGCCCAACGTGCTGGTTGGACGGCAATTGTCAGCCACCGCTCAGGCGAAAGCGAAGATGTAA
CGGTAGCCGATTTGGTGGTTGCCACCAACGCTGGTCAAATCAAAACAGGGGCACCAGCCCGTACCGACCGCGTAGCCAAA
TACAACCAATTGCTGCGGATCGAAGAAGAACTCGATAGCCAAGCCAAATTCGCTGGCTGGAGCGCCTTCGCCGTCAAACG
CGGCTAA

Upstream 100 bases:

>100_bases
AATTTATGCTCGATAGGTCGCGAAACCGCTACCCAGCGAATGGACAGAGCGTGGTATGATGGGGCATAAACATTCGTGTC
CCTTGAGGAGGCTTCTCAAT

Downstream 100 bases:

>100_bases
TCGATACTGACCATTGAGGACACGCAACGGCGCGTGTCCGCTTGCTATTTTAGGGGGAGCAAGCATGGCCGACGATCGCA
AAGCACTTGGGCGTTGGGGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MALITHVRGREVLDSRGNPTVEVEIGLEDGTLARAIVPSGASTGAHEAVELRDGDKSRYGGKGVLKAVKHVNEDIAEAIE
GLEALDQITLDKTLIELDGTKNKGKLGANAILGVSLATAKAGALSVGLPLYRYLGGVFGHTLPVPMMNILNGGKHAQDST
DFQEFMVMPVGAPSFREALRWGAEIYHTLKNVLHDRGLATNTGDEGGFAPSLPSNEAALEVIVEAIQKAGYTPGKDVMLA
LDPACSEIYENGIYNLAREGRKLSSEEMVGYWENLVNKYPIISIEDGLHEDDWGAWSSLRQRIGDRVQLVGDDLLVTNVE
RLNRAIKENSANSILIKLNQIGTLTETLDAIQMAQRAGWTAIVSHRSGESEDVTVADLVVATNAGQIKTGAPARTDRVAK
YNQLLRIEEELDSQAKFAGWSAFAVKRG

Sequences:

>Translated_428_residues
MALITHVRGREVLDSRGNPTVEVEIGLEDGTLARAIVPSGASTGAHEAVELRDGDKSRYGGKGVLKAVKHVNEDIAEAIE
GLEALDQITLDKTLIELDGTKNKGKLGANAILGVSLATAKAGALSVGLPLYRYLGGVFGHTLPVPMMNILNGGKHAQDST
DFQEFMVMPVGAPSFREALRWGAEIYHTLKNVLHDRGLATNTGDEGGFAPSLPSNEAALEVIVEAIQKAGYTPGKDVMLA
LDPACSEIYENGIYNLAREGRKLSSEEMVGYWENLVNKYPIISIEDGLHEDDWGAWSSLRQRIGDRVQLVGDDLLVTNVE
RLNRAIKENSANSILIKLNQIGTLTETLDAIQMAQRAGWTAIVSHRSGESEDVTVADLVVATNAGQIKTGAPARTDRVAK
YNQLLRIEEELDSQAKFAGWSAFAVKRG
>Mature_427_residues
ALITHVRGREVLDSRGNPTVEVEIGLEDGTLARAIVPSGASTGAHEAVELRDGDKSRYGGKGVLKAVKHVNEDIAEAIEG
LEALDQITLDKTLIELDGTKNKGKLGANAILGVSLATAKAGALSVGLPLYRYLGGVFGHTLPVPMMNILNGGKHAQDSTD
FQEFMVMPVGAPSFREALRWGAEIYHTLKNVLHDRGLATNTGDEGGFAPSLPSNEAALEVIVEAIQKAGYTPGKDVMLAL
DPACSEIYENGIYNLAREGRKLSSEEMVGYWENLVNKYPIISIEDGLHEDDWGAWSSLRQRIGDRVQLVGDDLLVTNVER
LNRAIKENSANSILIKLNQIGTLTETLDAIQMAQRAGWTAIVSHRSGESEDVTVADLVVATNAGQIKTGAPARTDRVAKY
NQLLRIEEELDSQAKFAGWSAFAVKRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=53.7383177570093, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI4503571, Length=428, Percent_Identity=53.0373831775701, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=51.8691588785047, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=51.8691588785047, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=47.1830985915493, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=26.5486725663717, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=26.5486725663717, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=26.5486725663717, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=57.311320754717, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=51.2820512820513, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=51.2820512820513, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=45.5026455026455, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=51.2761020881671, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=51.2761020881671, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=51.2761020881671, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=51.0440835266821, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=50.3480278422274, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=50.8120649651972, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=50.8120649651972, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=50.8120649651972, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=50.8120649651972, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=50.8120649651972, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=50.8120649651972, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46133; Mature: 46002

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALITHVRGREVLDSRGNPTVEVEIGLEDGTLARAIVPSGASTGAHEAVELRDGDKSRYG
CCEEECCCCHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVKHVNEDIAEAIEGLEALDQITLDKTLIELDGTKNKGKLGANAILGVSLATAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHEEEEHHHHC
AGALSVGLPLYRYLGGVFGHTLPVPMMNILNGGKHAQDSTDFQEFMVMPVGAPSFREALR
CCCHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
WGAEIYHTLKNVLHDRGLATNTGDEGGFAPSLPSNEAALEVIVEAIQKAGYTPGKDVMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDPACSEIYENGIYNLAREGRKLSSEEMVGYWENLVNKYPIISIEDGLHEDDWGAWSSLR
ECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
QRIGDRVQLVGDDLLVTNVERLNRAIKENSANSILIKLNQIGTLTETLDAIQMAQRAGWT
HHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCE
AIVSHRSGESEDVTVADLVVATNAGQIKTGAPARTDRVAKYNQLLRIEEELDSQAKFAGW
EEEECCCCCCCCEEEEHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCC
SAFAVKRG
EEEEEECC
>Mature Secondary Structure 
ALITHVRGREVLDSRGNPTVEVEIGLEDGTLARAIVPSGASTGAHEAVELRDGDKSRYG
CEEECCCCHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVKHVNEDIAEAIEGLEALDQITLDKTLIELDGTKNKGKLGANAILGVSLATAK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHEEEEHHHHC
AGALSVGLPLYRYLGGVFGHTLPVPMMNILNGGKHAQDSTDFQEFMVMPVGAPSFREALR
CCCHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
WGAEIYHTLKNVLHDRGLATNTGDEGGFAPSLPSNEAALEVIVEAIQKAGYTPGKDVMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDPACSEIYENGIYNLAREGRKLSSEEMVGYWENLVNKYPIISIEDGLHEDDWGAWSSLR
ECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
QRIGDRVQLVGDDLLVTNVERLNRAIKENSANSILIKLNQIGTLTETLDAIQMAQRAGWT
HHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCE
AIVSHRSGESEDVTVADLVVATNAGQIKTGAPARTDRVAKYNQLLRIEEELDSQAKFAGW
EEEECCCCCCCCEEEEHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCC
SAFAVKRG
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA