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The map label for this gene is sigA [H]

Identifier: 159896548

GI number: 159896548

Start: 18166

End: 19368

Strand: Direct

Name: sigA [H]

Synonym: Haur_0015

Alternate gene names: 159896548

Gene position: 18166-19368 (Clockwise)

Preceding gene: 159896547

Following gene: 159896549

Centisome position: 0.29

GC content: 51.29

Gene sequence:

>1203_bases
ATGGCTCTTGACGATGAAATCCTCGACTCTGAGCTTGCGGGTGATGAAACTGACGATTTTCCCGCTTTACGCACACTCAG
CGATCTTTTAGCCACTGGCAAAAAACGGGGCTATGTTACGCCTGGCGAAATTCAGCAAATTATCGATTCGCAGCGTGACG
AGGATAAACAACTCGAAGAAATTCGCGATACCTTGCAAAAGGCCAATATTCGAGTCGAGGAAACTGGCGACGACGACGAT
GAAATTACGGTCAGCGAAAACCCTGAAGCCGATTTTTCCAATGAGGGCATCAGTGTCAACGACACCGTGCGCTTATATTT
GCGCGAAATCGGCTTAGTGCCTTTGCTCAAAGGCGAGCAAGAGGTTGAGCTAGCCCGTGCTATGGAAGATGGCGATAAAG
CCCAACAAGAGTTGATCGAGAACGATCATACGCTTTCGGATGTTGAACGCCTAGCCTTGCGCCGCCGCATCGACCGTGGC
GAGCAAGCTCGTGAACACTTGACCACCGCCAACTTACGTTTGGTGGTCAGTGTGGCCAAAAAATACATTGGCCGTGGGCT
TTCGTTACTCGATTTGATTCAAGAAGGCAATGTTGGCTTGATTCGCGCGGTCGAGAAATTCAACTACACCAAAGGCTTTA
AGTTCTCTACCTACGCCACTTGGTGGATTCGCCAAGCAATCACGCGGGCAATCGCCGACCAAGCCCGTACCATTCGGATT
CCGGTGCACATGGTCGAAACGATCAACCGTATGATGCGCACAGCACGGCGTTTAACCCAAGAGAATGGCCGCGAACCTAG
CGATGAAGAACTGGCCCAGGCACTCGATCTGACGGTCGAGAAAGTGCGATCAATTCGCAAAACCTCGATGGAGCCAGTGT
CGCTCGAAACCCCAGTTGGTCAAGAAGAAGATAGCCAACTTGGCGACTTCTTGCCCGATGAAAAACTTGAAGCGCCTTCC
GATGCAGCTTCGCATCAGATGTTGCGCGAACAAGTTGCTCAGGTACTCGATCAACTGACCGAGCGTGAAAAGCGCGTGTT
GAAGCTACGCTTTGGGCTAGAAGATGGAACCCAACGCACACTCGAAGAAGTTGGCAAAGAATTTGGAGTAACTCGCGAAC
GAATTCGCCAGATCGAGGTGAAGGCGCTGCGCAAACTGCGTCACCCGCGCTTTGGCAAAAAACTCCGCGATTATCTGGAA
TAA

Upstream 100 bases:

>100_bases
CATGGCCATACAACATTATTTGCGTGCGACTTCGGAACCGCGTCGCAGCAGCTATTTTCTTGATACCCGTGATTCGCTCA
AATAATTAGAGGAGGAACAG

Downstream 100 bases:

>100_bases
ATCGATTTAATATTAACCACGAAGGACACGAAGAAACACGAAGATAGGTACTTCGCGATCTTCACGTTCTTCGTGGTTAC
AAACCTTGCTTCTCACTCGG

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MALDDEILDSELAGDETDDFPALRTLSDLLATGKKRGYVTPGEIQQIIDSQRDEDKQLEEIRDTLQKANIRVEETGDDDD
EITVSENPEADFSNEGISVNDTVRLYLREIGLVPLLKGEQEVELARAMEDGDKAQQELIENDHTLSDVERLALRRRIDRG
EQAREHLTTANLRLVVSVAKKYIGRGLSLLDLIQEGNVGLIRAVEKFNYTKGFKFSTYATWWIRQAITRAIADQARTIRI
PVHMVETINRMMRTARRLTQENGREPSDEELAQALDLTVEKVRSIRKTSMEPVSLETPVGQEEDSQLGDFLPDEKLEAPS
DAASHQMLREQVAQVLDQLTEREKRVLKLRFGLEDGTQRTLEEVGKEFGVTRERIRQIEVKALRKLRHPRFGKKLRDYLE

Sequences:

>Translated_400_residues
MALDDEILDSELAGDETDDFPALRTLSDLLATGKKRGYVTPGEIQQIIDSQRDEDKQLEEIRDTLQKANIRVEETGDDDD
EITVSENPEADFSNEGISVNDTVRLYLREIGLVPLLKGEQEVELARAMEDGDKAQQELIENDHTLSDVERLALRRRIDRG
EQAREHLTTANLRLVVSVAKKYIGRGLSLLDLIQEGNVGLIRAVEKFNYTKGFKFSTYATWWIRQAITRAIADQARTIRI
PVHMVETINRMMRTARRLTQENGREPSDEELAQALDLTVEKVRSIRKTSMEPVSLETPVGQEEDSQLGDFLPDEKLEAPS
DAASHQMLREQVAQVLDQLTEREKRVLKLRFGLEDGTQRTLEEVGKEFGVTRERIRQIEVKALRKLRHPRFGKKLRDYLE
>Mature_399_residues
ALDDEILDSELAGDETDDFPALRTLSDLLATGKKRGYVTPGEIQQIIDSQRDEDKQLEEIRDTLQKANIRVEETGDDDDE
ITVSENPEADFSNEGISVNDTVRLYLREIGLVPLLKGEQEVELARAMEDGDKAQQELIENDHTLSDVERLALRRRIDRGE
QAREHLTTANLRLVVSVAKKYIGRGLSLLDLIQEGNVGLIRAVEKFNYTKGFKFSTYATWWIRQAITRAIADQARTIRIP
VHMVETINRMMRTARRLTQENGREPSDEELAQALDLTVEKVRSIRKTSMEPVSLETPVGQEEDSQLGDFLPDEKLEAPSD
AASHQMLREQVAQVLDQLTEREKRVLKLRFGLEDGTQRTLEEVGKEFGVTRERIRQIEVKALRKLRHPRFGKKLRDYLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=252, Percent_Identity=58.7301587301587, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1789098, Length=289, Percent_Identity=44.2906574394464, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1789871, Length=299, Percent_Identity=26.7558528428094, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788231, Length=205, Percent_Identity=27.8048780487805, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 45759; Mature: 45628

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDDEILDSELAGDETDDFPALRTLSDLLATGKKRGYVTPGEIQQIIDSQRDEDKQLEE
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
IRDTLQKANIRVEETGDDDDEITVSENPEADFSNEGISVNDTVRLYLREIGLVPLLKGEQ
HHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
EVELARAMEDGDKAQQELIENDHTLSDVERLALRRRIDRGEQAREHLTTANLRLVVSVAK
HHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KYIGRGLSLLDLIQEGNVGLIRAVEKFNYTKGFKFSTYATWWIRQAITRAIADQARTIRI
HHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEC
PVHMVETINRMMRTARRLTQENGREPSDEELAQALDLTVEKVRSIRKTSMEPVSLETPVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QEEDSQLGDFLPDEKLEAPSDAASHQMLREQVAQVLDQLTEREKRVLKLRFGLEDGTQRT
CCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LEEVGKEFGVTRERIRQIEVKALRKLRHPRFGKKLRDYLE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
ALDDEILDSELAGDETDDFPALRTLSDLLATGKKRGYVTPGEIQQIIDSQRDEDKQLEE
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
IRDTLQKANIRVEETGDDDDEITVSENPEADFSNEGISVNDTVRLYLREIGLVPLLKGEQ
HHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
EVELARAMEDGDKAQQELIENDHTLSDVERLALRRRIDRGEQAREHLTTANLRLVVSVAK
HHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KYIGRGLSLLDLIQEGNVGLIRAVEKFNYTKGFKFSTYATWWIRQAITRAIADQARTIRI
HHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEC
PVHMVETINRMMRTARRLTQENGREPSDEELAQALDLTVEKVRSIRKTSMEPVSLETPVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QEEDSQLGDFLPDEKLEAPSDAASHQMLREQVAQVLDQLTEREKRVLKLRFGLEDGTQRT
CCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LEEVGKEFGVTRERIRQIEVKALRKLRHPRFGKKLRDYLE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]