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The map label for this gene is dnaG [H]

Identifier: 159896547

GI number: 159896547

Start: 16213

End: 18150

Strand: Direct

Name: dnaG [H]

Synonym: Haur_0014

Alternate gene names: 159896547

Gene position: 16213-18150 (Clockwise)

Preceding gene: 159896546

Following gene: 159896548

Centisome position: 0.26

GC content: 52.63

Gene sequence:

>1938_bases
ATGAACTCAGTTATCGACGATATTAAAGAACGCATTGACATTGTGTCGTTCATTAGCGATTATGTGCCGCTGCGTAAGGC
GGGGCGCAATTGGGTGGGCTTTTGCCCATTCCATTCGAATACCCGCACGCCAGCTTTTACGGTGTTTGCCGATAGTAATA
GCTACCACTGTTTTGGCTGCAAAGCCAGCGGCACAATTTTTGATTTTCTGATGCAGCGCGAGGGCATCGACTTCCCTGAG
GCACTGAATCAACTTGCGGCCCGCGCAGGAGTGCAATTACGCCAGCGTACCGCCCAAGATGACCAAGAAGATGTGCTGCG
TTCGCGCATGCTGGAGTTGAACGCCGCCGCTGCCCGTTTTTGGAGTCATCAATTATTACAATCGCCCAAAGCGGCTCATG
TGCGTAGCTACATCGAGCAACGTGGGCTTACCCCGCAAACTGTTGAGCAATTTCAGTTGGGCTATGCTAGCGAAGATTGG
TCAAGTTTGCTGGGTTACTTGCACGACCGCCACGGAGCCAATCCGAGTGAAGTGGCCGAGGTTGGCTTAGCCTTGGAACG
TGAACAGGGCGGCTATTATGATCGCTTTCGTGGGCGTTTGATGTTTCCAATTCATAATGCCAAGGGCCAGATTGTAGGCT
TTGGTGGGCGGATTTTGGGCGATGGCCACCCCAAATATATCAACTCGCCGCAAACCTTGCTGTTTGATAAAAGTAGCCTG
TTGTATGGGCTATTTCAAGCCCGCGAGACGATTCGCAGCACTGATAGCGTGGTGGTGGTTGAGGGCTATGTTGATGTGAT
TATGGCCCATCAAGCGGGCTTTCGCAATGTGCTAGCGCCGATGGGCACAGCCTTGACCGATGTGCATGCCGGCCAACTCA
ACAAAATGACCAAGCGCATCACATTGGCAATGGATGGCGATGCTGCTGGCCAGTCGGCGGCCTTGCGTGGCTTGGAAACC
TTGCGTGAAACGCTCGATACTCATGTGCGGCCTGTGCCAACTGCTTCGGGCATGTTGCGCTGGGAGCGCGAGCTTGATGC
CACCATCAAGATTGCCCTGCTGCCTGATGGGCGTGACCCTGATGATATTGTGCGCAACAACCCTGAGGAATGGCGCTCGC
TGATTGCCAATGCTCAGCCGTTGATGGATTTTTATCTGGCAACTTTGACGAGAGGCTTGGATTTGAATAGTGCCAAAGGC
AAATCGACAGCAGTCGAGCGCCTTACGCCTTTGCTCAATCAAATTGCCGATCCAGTTGAAAAAGCCCATTATATTCAGCG
GCTTGCCAACCAGATCAAGATCGATCAGCGATTAATTGAAGGATCAATTGGTGGCGACCAACCAGAGCGTCAGCGCAAAC
CCAACCATCGTCAAAGCCGCCCCGCTGCACCACCACCAGTTTTTTCAATCTTAGAGCAGGAGGCACGGCGCGAAGATTTT
CTTTTATCGTTATTAATTCGCTTTCCCCAAGTGCAAGCAGTGGTTAGCGAACAACTACGTGATGATTTAGCCGCAGCGCC
AACCCTGCGCGAGTTGTGGAGTGGCGATGTGAGCGAACTTTTTGAACGAGTCGAAAATCGTGTATTATGGGATCTGTGGC
GCTCAGCCCAGGCGATGGCTACTCCCAATCCAATTGAATGGCTTGAAACGGTACCTGCCGAATTGCAAGCCCATGGCGAA
CGCCTACTTAATTGGGATCATGCTCCACCAACCCGTAGTTTCCGTGTGACTCGTGAGGCCGAGGAATGTTTGCGCCCGTT
GCGCCACCGTTTAGGCAAGCGCTGGAGCGACCGCCTTGGTCAGATAATTGCCGCCGCCGAACCTGATCAACAGGAACGAT
TGCTGGAGCAAGCCATGGCCATACAACATTATTTGCGTGCGACTTCGGAACCGCGTCGCAGCAGCTATTTTCTTGATACC
CGTGATTCGCTCAAATAA

Upstream 100 bases:

>100_bases
GACTAGCTTTAAATGCTGGTTTTGTGCTCTTCGTGGTTAAAAAAGGAGCCAATGATAGGTATAGCGCCGAACATACCTAT
GTTCTATGCTCTAAACAATT

Downstream 100 bases:

>100_bases
TTAGAGGAGGAACAGATGGCTCTTGACGATGAAATCCTCGACTCTGAGCTTGCGGGTGATGAAACTGACGATTTTCCCGC
TTTACGCACACTCAGCGATC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MNSVIDDIKERIDIVSFISDYVPLRKAGRNWVGFCPFHSNTRTPAFTVFADSNSYHCFGCKASGTIFDFLMQREGIDFPE
ALNQLAARAGVQLRQRTAQDDQEDVLRSRMLELNAAAARFWSHQLLQSPKAAHVRSYIEQRGLTPQTVEQFQLGYASEDW
SSLLGYLHDRHGANPSEVAEVGLALEREQGGYYDRFRGRLMFPIHNAKGQIVGFGGRILGDGHPKYINSPQTLLFDKSSL
LYGLFQARETIRSTDSVVVVEGYVDVIMAHQAGFRNVLAPMGTALTDVHAGQLNKMTKRITLAMDGDAAGQSAALRGLET
LRETLDTHVRPVPTASGMLRWERELDATIKIALLPDGRDPDDIVRNNPEEWRSLIANAQPLMDFYLATLTRGLDLNSAKG
KSTAVERLTPLLNQIADPVEKAHYIQRLANQIKIDQRLIEGSIGGDQPERQRKPNHRQSRPAAPPPVFSILEQEARREDF
LLSLLIRFPQVQAVVSEQLRDDLAAAPTLRELWSGDVSELFERVENRVLWDLWRSAQAMATPNPIEWLETVPAELQAHGE
RLLNWDHAPPTRSFRVTREAEECLRPLRHRLGKRWSDRLGQIIAAAEPDQQERLLEQAMAIQHYLRATSEPRRSSYFLDT
RDSLK

Sequences:

>Translated_645_residues
MNSVIDDIKERIDIVSFISDYVPLRKAGRNWVGFCPFHSNTRTPAFTVFADSNSYHCFGCKASGTIFDFLMQREGIDFPE
ALNQLAARAGVQLRQRTAQDDQEDVLRSRMLELNAAAARFWSHQLLQSPKAAHVRSYIEQRGLTPQTVEQFQLGYASEDW
SSLLGYLHDRHGANPSEVAEVGLALEREQGGYYDRFRGRLMFPIHNAKGQIVGFGGRILGDGHPKYINSPQTLLFDKSSL
LYGLFQARETIRSTDSVVVVEGYVDVIMAHQAGFRNVLAPMGTALTDVHAGQLNKMTKRITLAMDGDAAGQSAALRGLET
LRETLDTHVRPVPTASGMLRWERELDATIKIALLPDGRDPDDIVRNNPEEWRSLIANAQPLMDFYLATLTRGLDLNSAKG
KSTAVERLTPLLNQIADPVEKAHYIQRLANQIKIDQRLIEGSIGGDQPERQRKPNHRQSRPAAPPPVFSILEQEARREDF
LLSLLIRFPQVQAVVSEQLRDDLAAAPTLRELWSGDVSELFERVENRVLWDLWRSAQAMATPNPIEWLETVPAELQAHGE
RLLNWDHAPPTRSFRVTREAEECLRPLRHRLGKRWSDRLGQIIAAAEPDQQERLLEQAMAIQHYLRATSEPRRSSYFLDT
RDSLK
>Mature_645_residues
MNSVIDDIKERIDIVSFISDYVPLRKAGRNWVGFCPFHSNTRTPAFTVFADSNSYHCFGCKASGTIFDFLMQREGIDFPE
ALNQLAARAGVQLRQRTAQDDQEDVLRSRMLELNAAAARFWSHQLLQSPKAAHVRSYIEQRGLTPQTVEQFQLGYASEDW
SSLLGYLHDRHGANPSEVAEVGLALEREQGGYYDRFRGRLMFPIHNAKGQIVGFGGRILGDGHPKYINSPQTLLFDKSSL
LYGLFQARETIRSTDSVVVVEGYVDVIMAHQAGFRNVLAPMGTALTDVHAGQLNKMTKRITLAMDGDAAGQSAALRGLET
LRETLDTHVRPVPTASGMLRWERELDATIKIALLPDGRDPDDIVRNNPEEWRSLIANAQPLMDFYLATLTRGLDLNSAKG
KSTAVERLTPLLNQIADPVEKAHYIQRLANQIKIDQRLIEGSIGGDQPERQRKPNHRQSRPAAPPPVFSILEQEARREDF
LLSLLIRFPQVQAVVSEQLRDDLAAAPTLRELWSGDVSELFERVENRVLWDLWRSAQAMATPNPIEWLETVPAELQAHGE
RLLNWDHAPPTRSFRVTREAEECLRPLRHRLGKRWSDRLGQIIAAAEPDQQERLLEQAMAIQHYLRATSEPRRSSYFLDT
RDSLK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=497, Percent_Identity=33.8028169014084, Blast_Score=269, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 72930; Mature: 72930

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVIDDIKERIDIVSFISDYVPLRKAGRNWVGFCPFHSNTRTPAFTVFADSNSYHCFGC
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCEEEEEC
KASGTIFDFLMQREGIDFPEALNQLAARAGVQLRQRTAQDDQEDVLRSRMLELNAAAARF
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
WSHQLLQSPKAAHVRSYIEQRGLTPQTVEQFQLGYASEDWSSLLGYLHDRHGANPSEVAE
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHH
VGLALEREQGGYYDRFRGRLMFPIHNAKGQIVGFGGRILGDGHPKYINSPQTLLFDKSSL
HHHHHHHCCCCCHHHHCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCEEECCHHH
LYGLFQARETIRSTDSVVVVEGYVDVIMAHQAGFRNVLAPMGTALTDVHAGQLNKMTKRI
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
TLAMDGDAAGQSAALRGLETLRETLDTHVRPVPTASGMLRWERELDATIKIALLPDGRDP
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCH
DDIVRNNPEEWRSLIANAQPLMDFYLATLTRGLDLNSAKGKSTAVERLTPLLNQIADPVE
HHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KAHYIQRLANQIKIDQRLIEGSIGGDQPERQRKPNHRQSRPAAPPPVFSILEQEARREDF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLSLLIRFPQVQAVVSEQLRDDLAAAPTLRELWSGDVSELFERVENRVLWDLWRSAQAMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
TPNPIEWLETVPAELQAHGERLLNWDHAPPTRSFRVTREAEECLRPLRHRLGKRWSDRLG
CCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QIIAAAEPDQQERLLEQAMAIQHYLRATSEPRRSSYFLDTRDSLK
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHCCC
>Mature Secondary Structure
MNSVIDDIKERIDIVSFISDYVPLRKAGRNWVGFCPFHSNTRTPAFTVFADSNSYHCFGC
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCEEEEEC
KASGTIFDFLMQREGIDFPEALNQLAARAGVQLRQRTAQDDQEDVLRSRMLELNAAAARF
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
WSHQLLQSPKAAHVRSYIEQRGLTPQTVEQFQLGYASEDWSSLLGYLHDRHGANPSEVAE
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHH
VGLALEREQGGYYDRFRGRLMFPIHNAKGQIVGFGGRILGDGHPKYINSPQTLLFDKSSL
HHHHHHHCCCCCHHHHCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCEEECCHHH
LYGLFQARETIRSTDSVVVVEGYVDVIMAHQAGFRNVLAPMGTALTDVHAGQLNKMTKRI
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
TLAMDGDAAGQSAALRGLETLRETLDTHVRPVPTASGMLRWERELDATIKIALLPDGRDP
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCH
DDIVRNNPEEWRSLIANAQPLMDFYLATLTRGLDLNSAKGKSTAVERLTPLLNQIADPVE
HHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KAHYIQRLANQIKIDQRLIEGSIGGDQPERQRKPNHRQSRPAAPPPVFSILEQEARREDF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLSLLIRFPQVQAVVSEQLRDDLAAAPTLRELWSGDVSELFERVENRVLWDLWRSAQAMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
TPNPIEWLETVPAELQAHGERLLNWDHAPPTRSFRVTREAEECLRPLRHRLGKRWSDRLG
CCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QIIAAAEPDQQERLLEQAMAIQHYLRATSEPRRSSYFLDTRDSLK
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]