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The map label for this gene is galE [C]

Identifier: 159041982

GI number: 159041982

Start: 1497777

End: 1498946

Strand: Reverse

Name: galE [C]

Synonym: Cmaq_1419

Alternate gene names: 159041982

Gene position: 1498946-1497777 (Counterclockwise)

Preceding gene: 159041984

Following gene: 159041979

Centisome position: 72.15

GC content: 47.69

Gene sequence:

>1170_bases
GTGAGGATTCTTATACTTGGTGTTGATGGTTACCTGGGTTGGGCACTTGCGCTTAGGCTTATAAGGAGGGGTCATGAGGT
TATTGGTGTTGATAACTTCTACACTAGGAGGGCTGTGGAGGAGGTTGGTTCATGGTCTGCTTTACCGATATTAAGCATGG
AGGATAGGGTTAAGGCTGTGGAGGAGGTTTATGGATCGAGGCTGGTTTTCCATGAGGCTGATGTGACTGATTACGATGCC
GTGAGGCACATTATTGAGAGGCATAGGCCTGATGCAATAGTTCACTTTGCTGAGCAACGTTCAGCACCATACTCAATGAT
TGATGTTAACCACGCCTCCTACACAATGGTGAATAACCTTAAGTCAACCCTCAACGTAATCTACTCAATAAGGGAGGTTA
ATGGTAGGATTCATTTACTTAAAATGGGTACACTGGGTGAGTTCGGTTACCCAGCCTTCAAGCTACCTGAGGACGCCTTC
GTTGACGCTGTGATTAATGGTGTTAAGGATAGGGTACTTGTACCGAGGTGGGGTGGTTCATGGTATCATTGGAGTAAGGT
CTTCGACTCTTACATGCTTGTTTACGCCAATAAGCTATGGGGATTAACCATAACTGACTTCCACCAGGGGCCGGTCTACG
GTACGAGGACCAGTGATATTGTTTCAGATGAATTATTCACTAGGTTTGACTTCGATGATGTATGGGGCACTGTGTTCAAT
AGGTTCTGCGCCGAGGCTGTGATTGGCCACGAGTTAACAGTCTACGGTACCGGGCTTCAGAGGAAGGGCTTCACCTCCCT
TGAAGACACCACCGAGGCCCTGACACTACTCCTGGAGAATCCCCCCAGTGACGGTGAGTATAGGACTGTTTACCATTATA
GGGAGGTGCTGACGCTTAACACCATAGCTGACTTGGTTAAGGAGGCTAGTAGGGAGGTTTTGGGTTATGAACCACAGGTG
GTTCACTTACCTAACCCAAGGGTGGAGCCTGAGGATGACTTAAACTATGAGCCTGAGAGGAGGGTTCTACCTAGGTTAGG
TATGTATGGGTTAAGGAGGAACATGAGGGATGAGGTTAAGGGTATATTAAGGGACTTAGCCAGGTATAGGGGTAGGATTG
AGGCTAAGGCTAGTGTACTTAAACCCAGGGTTAAATGGAGGGGGAGTTAG

Upstream 100 bases:

>100_bases
AGTCCCTGGATCCAAGAGTATTCGCAATCATCATTATGGCTGCGCTTATATTAACTTGAGGTGAGTTAATTATAAATAAC
TAGAGGGTGTACGAGTCATT

Downstream 100 bases:

>100_bases
GTTGAATCACTGTTGACGGGGGAGGACCCGTAGGCATTTATGCAGCTTAAGCACCTCAAGTTCAGTGTTTATCCCATCAA
TGACATCATCATTAACCTCA

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Epimerase; UDP Sulfoquinovose Synthase; Nucleoside-Diphosphate-Sugar Epimerase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRILILGVDGYLGWALALRLIRRGHEVIGVDNFYTRRAVEEVGSWSALPILSMEDRVKAVEEVYGSRLVFHEADVTDYDA
VRHIIERHRPDAIVHFAEQRSAPYSMIDVNHASYTMVNNLKSTLNVIYSIREVNGRIHLLKMGTLGEFGYPAFKLPEDAF
VDAVINGVKDRVLVPRWGGSWYHWSKVFDSYMLVYANKLWGLTITDFHQGPVYGTRTSDIVSDELFTRFDFDDVWGTVFN
RFCAEAVIGHELTVYGTGLQRKGFTSLEDTTEALTLLLENPPSDGEYRTVYHYREVLTLNTIADLVKEASREVLGYEPQV
VHLPNPRVEPEDDLNYEPERRVLPRLGMYGLRRNMRDEVKGILRDLARYRGRIEAKASVLKPRVKWRGS

Sequences:

>Translated_389_residues
MRILILGVDGYLGWALALRLIRRGHEVIGVDNFYTRRAVEEVGSWSALPILSMEDRVKAVEEVYGSRLVFHEADVTDYDA
VRHIIERHRPDAIVHFAEQRSAPYSMIDVNHASYTMVNNLKSTLNVIYSIREVNGRIHLLKMGTLGEFGYPAFKLPEDAF
VDAVINGVKDRVLVPRWGGSWYHWSKVFDSYMLVYANKLWGLTITDFHQGPVYGTRTSDIVSDELFTRFDFDDVWGTVFN
RFCAEAVIGHELTVYGTGLQRKGFTSLEDTTEALTLLLENPPSDGEYRTVYHYREVLTLNTIADLVKEASREVLGYEPQV
VHLPNPRVEPEDDLNYEPERRVLPRLGMYGLRRNMRDEVKGILRDLARYRGRIEAKASVLKPRVKWRGS
>Mature_389_residues
MRILILGVDGYLGWALALRLIRRGHEVIGVDNFYTRRAVEEVGSWSALPILSMEDRVKAVEEVYGSRLVFHEADVTDYDA
VRHIIERHRPDAIVHFAEQRSAPYSMIDVNHASYTMVNNLKSTLNVIYSIREVNGRIHLLKMGTLGEFGYPAFKLPEDAF
VDAVINGVKDRVLVPRWGGSWYHWSKVFDSYMLVYANKLWGLTITDFHQGPVYGTRTSDIVSDELFTRFDFDDVWGTVFN
RFCAEAVIGHELTVYGTGLQRKGFTSLEDTTEALTLLLENPPSDGEYRTVYHYREVLTLNTIADLVKEASREVLGYEPQV
VHLPNPRVEPEDDLNYEPERRVLPRLGMYGLRRNMRDEVKGILRDLARYRGRIEAKASVLKPRVKWRGS

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 44700; Mature: 44700

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILILGVDGYLGWALALRLIRRGHEVIGVDNFYTRRAVEEVGSWSALPILSMEDRVKAV
CEEEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEECCHHHHHHHH
EEVYGSRLVFHEADVTDYDAVRHIIERHRPDAIVHFAEQRSAPYSMIDVNHASYTMVNNL
HHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEHHHCCCCEEEEECCCCHHHHHHHH
KSTLNVIYSIREVNGRIHLLKMGTLGEFGYPAFKLPEDAFVDAVINGVKDRVLVPRWGGS
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCC
WYHWSKVFDSYMLVYANKLWGLTITDFHQGPVYGTRTSDIVSDELFTRFDFDDVWGTVFN
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
RFCAEAVIGHELTVYGTGLQRKGFTSLEDTTEALTLLLENPPSDGEYRTVYHYREVLTLN
HHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHH
TIADLVKEASREVLGYEPQVVHLPNPRVEPEDDLNYEPERRVLPRLGMYGLRRNMRDEVK
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GILRDLARYRGRIEAKASVLKPRVKWRGS
HHHHHHHHHCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRILILGVDGYLGWALALRLIRRGHEVIGVDNFYTRRAVEEVGSWSALPILSMEDRVKAV
CEEEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCEECCHHHHHHHH
EEVYGSRLVFHEADVTDYDAVRHIIERHRPDAIVHFAEQRSAPYSMIDVNHASYTMVNNL
HHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEHHHCCCCEEEEECCCCHHHHHHHH
KSTLNVIYSIREVNGRIHLLKMGTLGEFGYPAFKLPEDAFVDAVINGVKDRVLVPRWGGS
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCC
WYHWSKVFDSYMLVYANKLWGLTITDFHQGPVYGTRTSDIVSDELFTRFDFDDVWGTVFN
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
RFCAEAVIGHELTVYGTGLQRKGFTSLEDTTEALTLLLENPPSDGEYRTVYHYREVLTLN
HHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHH
TIADLVKEASREVLGYEPQVVHLPNPRVEPEDDLNYEPERRVLPRLGMYGLRRNMRDEVK
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GILRDLARYRGRIEAKASVLKPRVKWRGS
HHHHHHHHHCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA