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The map label for this gene is cofH [H]

Identifier: 159041979

GI number: 159041979

Start: 1494760

End: 1495836

Strand: Reverse

Name: cofH [H]

Synonym: Cmaq_1416

Alternate gene names: 159041979

Gene position: 1495836-1494760 (Counterclockwise)

Preceding gene: 159041982

Following gene: 159041975

Centisome position: 72.0

GC content: 46.33

Gene sequence:

>1077_bases
ATGAGTCAGGCATTGGAGAAGGCCCTTAGTGGTGAAGAGTTGAAGCCCCCTGATATTGAGGAGCTTCTTGAATTAAACCT
ATGGGACCTAGGCATCGCTGCAGGGGAGTTAACTAGGAGGTTGTTTAATGGTGTGGTGACCTTCATACCCAATCTAATAC
TGAACTACACCAACGTCTGCACAATAGCCTGCAGCTTCTGCGCCTTCTATAGGCCACCTAGGCACCCTGAGGCATACACC
ATGAGTGTGGATTACGCCGTTAAGCTTGTTACAGAGGTTGATTCAAGATTCGGTATTAGGCAGGTTCTAGTGCAGGGTGG
TATTAATCCTGAATTAGGCATTGAGTACTACGAGGAATTATTCAAGACCCTTAAGGCTAAGCTACCCCACGTGGCTATTC
ACGGTTTAAGCCCCATTGAGGTTGATTACCTAGCTAGGAAGCATAGAATGAGTTACAGGGAGGTTCTTGAGAGGCTTAAG
GCAGCTGGAATGGATACGCTAGCTGGGGGTGGCGGGGAGATTCTGGTGGATGAGGTTAGGAGGATTATTGCACCACATAA
GATTAGTGCTGAAACCTGGTTGAGAGTAATGGAGATTGCCCACGGGTTAGGCATAATGAGTAACGCAACAATGATGTACG
GGCACGTGGAGTCTAAGGCGCATTGGGCTGAGCACCTATACAGGATTATTAGCCTACAGAGGAGGACTCATGGATTCCTA
TCCTTCACCGCGTGGAATTTCGAGCCAGGTAACAGTGAGTTAACTAATAAGGTTCCTTACCCATTAACATCAGCCACATT
ACTGAGGGTGGTTGCCGTGGCTAGGCTTGTGTTTAAGGGTGAGTTACCTAATATTCAATCAAGTTGGTTAACTAATGGTC
TTGATACTGCTCAATTAGCCCTCAAATTCGGTGCAAACGACTTCGGTGGAACACTATACGAGGAGAGAGTAATACCAGCA
ACCGGGTTAAGTAAACCAGTATTCACAAGGGATTACGTAATCAACATGATTAGAAGCCTAGGATACAAACCAGCGGAACG
CGACAACTGGTATAGGGTGTTAAAACTATACGACTAA

Upstream 100 bases:

>100_bases
TTGACTACCCATTGGACTAACTTAAAAAACTTAACCCAATAAACCCAAGGAAGCGAACCCTTGAGTTAAAGTTAAAGAAC
CTAATTTAACCCAATCCTCA

Downstream 100 bases:

>100_bases
AGTAACCTAATTCTTAAGGAATTAATGTTAAGCCTAAAATCCACTTTAAGGGGATTAGGCACATTGATGATGAGTTAGGC
CTCATTTAAGAGGGGGTGAA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSQALEKALSGEELKPPDIEELLELNLWDLGIAAGELTRRLFNGVVTFIPNLILNYTNVCTIACSFCAFYRPPRHPEAYT
MSVDYAVKLVTEVDSRFGIRQVLVQGGINPELGIEYYEELFKTLKAKLPHVAIHGLSPIEVDYLARKHRMSYREVLERLK
AAGMDTLAGGGGEILVDEVRRIIAPHKISAETWLRVMEIAHGLGIMSNATMMYGHVESKAHWAEHLYRIISLQRRTHGFL
SFTAWNFEPGNSELTNKVPYPLTSATLLRVVAVARLVFKGELPNIQSSWLTNGLDTAQLALKFGANDFGGTLYEERVIPA
TGLSKPVFTRDYVINMIRSLGYKPAERDNWYRVLKLYD

Sequences:

>Translated_358_residues
MSQALEKALSGEELKPPDIEELLELNLWDLGIAAGELTRRLFNGVVTFIPNLILNYTNVCTIACSFCAFYRPPRHPEAYT
MSVDYAVKLVTEVDSRFGIRQVLVQGGINPELGIEYYEELFKTLKAKLPHVAIHGLSPIEVDYLARKHRMSYREVLERLK
AAGMDTLAGGGGEILVDEVRRIIAPHKISAETWLRVMEIAHGLGIMSNATMMYGHVESKAHWAEHLYRIISLQRRTHGFL
SFTAWNFEPGNSELTNKVPYPLTSATLLRVVAVARLVFKGELPNIQSSWLTNGLDTAQLALKFGANDFGGTLYEERVIPA
TGLSKPVFTRDYVINMIRSLGYKPAERDNWYRVLKLYD
>Mature_357_residues
SQALEKALSGEELKPPDIEELLELNLWDLGIAAGELTRRLFNGVVTFIPNLILNYTNVCTIACSFCAFYRPPRHPEAYTM
SVDYAVKLVTEVDSRFGIRQVLVQGGINPELGIEYYEELFKTLKAKLPHVAIHGLSPIEVDYLARKHRMSYREVLERLKA
AGMDTLAGGGGEILVDEVRRIIAPHKISAETWLRVMEIAHGLGIMSNATMMYGHVESKAHWAEHLYRIISLQRRTHGFLS
FTAWNFEPGNSELTNKVPYPLTSATLLRVVAVARLVFKGELPNIQSSWLTNGLDTAQLALKFGANDFGGTLYEERVIPAT
GLSKPVFTRDYVINMIRSLGYKPAERDNWYRVLKLYD

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40373; Mature: 40242

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALEKALSGEELKPPDIEELLELNLWDLGIAAGELTRRLFNGVVTFIPNLILNYTNVC
CCHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIACSFCAFYRPPRHPEAYTMSVDYAVKLVTEVDSRFGIRQVLVQGGINPELGIEYYEEL
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FKTLKAKLPHVAIHGLSPIEVDYLARKHRMSYREVLERLKAAGMDTLAGGGGEILVDEVR
HHHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RIIAPHKISAETWLRVMEIAHGLGIMSNATMMYGHVESKAHWAEHLYRIISLQRRTHGFL
HHHCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCEE
SFTAWNFEPGNSELTNKVPYPLTSATLLRVVAVARLVFKGELPNIQSSWLTNGLDTAQLA
EEEEECCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHH
LKFGANDFGGTLYEERVIPATGLSKPVFTRDYVINMIRSLGYKPAERDNWYRVLKLYD
HHHCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SQALEKALSGEELKPPDIEELLELNLWDLGIAAGELTRRLFNGVVTFIPNLILNYTNVC
CHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIACSFCAFYRPPRHPEAYTMSVDYAVKLVTEVDSRFGIRQVLVQGGINPELGIEYYEEL
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FKTLKAKLPHVAIHGLSPIEVDYLARKHRMSYREVLERLKAAGMDTLAGGGGEILVDEVR
HHHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RIIAPHKISAETWLRVMEIAHGLGIMSNATMMYGHVESKAHWAEHLYRIISLQRRTHGFL
HHHCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCEE
SFTAWNFEPGNSELTNKVPYPLTSATLLRVVAVARLVFKGELPNIQSSWLTNGLDTAQLA
EEEEECCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHH
LKFGANDFGGTLYEERVIPATGLSKPVFTRDYVINMIRSLGYKPAERDNWYRVLKLYD
HHHCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]