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The map label for this gene is rutB [H]

Identifier: 159041900

GI number: 159041900

Start: 1412237

End: 1412851

Strand: Reverse

Name: rutB [H]

Synonym: Cmaq_1336

Alternate gene names: 159041900

Gene position: 1412851-1412237 (Counterclockwise)

Preceding gene: 159041901

Following gene: 159041898

Centisome position: 68.01

GC content: 42.44

Gene sequence:

>615_bases
ATGAGGATTAAGGAGACTGTTGAGGTTCCTGAACTTGAGGTTAAGGAGAGTATTACTGTTGATTCATCAAGGTCCGCGGT
ATTAGTTATTGATATGCAGAATGACTTCGTTAGAAGTAATGGTAAGCTTTATGTGCCGGATGCTGAAGAAACCATTAAAC
CCATTGCCGACTTACTTACAAGGGCTAGGAGAAGTGGGGTAAGGGTGATTTACACTCAGGACTGGCACTTTAAGGATGAT
CCGGAGTTTAGGATATGGGGTGAGCACTGTGTTATGGATACTTGGGGTGCTGAAATTATTGATGAACTTAAGCCGCAGCC
TGGTGATGTAGTTATTAGAAAGAGGAGGTATGATGCCTTCTTCGGCACTGACTTGGATTACGTGCTTAGGCATGTTGTTA
AGGCTGATACATTAATAATAACCGGCACAGTGGCTAACATATGCGTACTCCATACGGCTGGTAGCGCTGCATTGAATTGG
TATAAGGTTATCATGCCTATTGATGCCGTGTCGGCGTTGAATAGGTTTGATTACTTGGCTTCATTAAGGCAAGTGAGCTT
CCTATATAAGGGGGATTTAACAACAGTGAATGGTATTGTTTTTAAGAACCAGTAA

Upstream 100 bases:

>100_bases
TCAGTATGATGAATACTGCACGTGCGGTAAGGAAGCCAGCTGGCTTTCAAGTAATTAACAGCAGTACAATGCTTTTAATT
AGGGCTAAGTCTCCTCACTC

Downstream 100 bases:

>100_bases
TGAATCATCTCCTGTATTGTTAATTCCAAGGTACTAAACCTATGCTTCACCAGAACCCTGATTTCCACTGCATTTAATTA
ACGCGTCATTTATTAGTTCA

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MRIKETVEVPELEVKESITVDSSRSAVLVIDMQNDFVRSNGKLYVPDAEETIKPIADLLTRARRSGVRVIYTQDWHFKDD
PEFRIWGEHCVMDTWGAEIIDELKPQPGDVVIRKRRYDAFFGTDLDYVLRHVVKADTLIITGTVANICVLHTAGSAALNW
YKVIMPIDAVSALNRFDYLASLRQVSFLYKGDLTTVNGIVFKNQ

Sequences:

>Translated_204_residues
MRIKETVEVPELEVKESITVDSSRSAVLVIDMQNDFVRSNGKLYVPDAEETIKPIADLLTRARRSGVRVIYTQDWHFKDD
PEFRIWGEHCVMDTWGAEIIDELKPQPGDVVIRKRRYDAFFGTDLDYVLRHVVKADTLIITGTVANICVLHTAGSAALNW
YKVIMPIDAVSALNRFDYLASLRQVSFLYKGDLTTVNGIVFKNQ
>Mature_204_residues
MRIKETVEVPELEVKESITVDSSRSAVLVIDMQNDFVRSNGKLYVPDAEETIKPIADLLTRARRSGVRVIYTQDWHFKDD
PEFRIWGEHCVMDTWGAEIIDELKPQPGDVVIRKRRYDAFFGTDLDYVLRHVVKADTLIITGTVANICVLHTAGSAALNW
YKVIMPIDAVSALNRFDYLASLRQVSFLYKGDLTTVNGIVFKNQ

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=201, Percent_Identity=29.3532338308458, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786811, Length=188, Percent_Identity=29.2553191489362, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321401, Length=187, Percent_Identity=30.4812834224599, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 23222; Mature: 23222

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKETVEVPELEVKESITVDSSRSAVLVIDMQNDFVRSNGKLYVPDAEETIKPIADLLT
CCCCCCCCCCCEECCCEEEECCCCCEEEEEECCCCHHHCCCEEEECCCHHHHHHHHHHHH
RARRSGVRVIYTQDWHFKDDPEFRIWGEHCVMDTWGAEIIDELKPQPGDVVIRKRRYDAF
HHHHCCEEEEEEECCCCCCCCCEEEECCEEEECHHHHHHHHHHCCCCCCEEEEEEECCEE
FGTDLDYVLRHVVKADTLIITGTVANICVLHTAGSAALNWYKVIMPIDAVSALNRFDYLA
CCCCHHHHHHHHHHHCEEEEEECHHEEEEEECCCCHHHEEEEEEECHHHHHHHHHHHHHH
SLRQVSFLYKGDLTTVNGIVFKNQ
HHHHHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRIKETVEVPELEVKESITVDSSRSAVLVIDMQNDFVRSNGKLYVPDAEETIKPIADLLT
CCCCCCCCCCCEECCCEEEECCCCCEEEEEECCCCHHHCCCEEEECCCHHHHHHHHHHHH
RARRSGVRVIYTQDWHFKDDPEFRIWGEHCVMDTWGAEIIDELKPQPGDVVIRKRRYDAF
HHHHCCEEEEEEECCCCCCCCCEEEECCEEEECHHHHHHHHHHCCCCCCEEEEEEECCEE
FGTDLDYVLRHVVKADTLIITGTVANICVLHTAGSAALNWYKVIMPIDAVSALNRFDYLA
CCCCHHHHHHHHHHHCEEEEEECHHEEEEEECCCCHHHEEEEEEECHHHHHHHHHHHHHH
SLRQVSFLYKGDLTTVNGIVFKNQ
HHHHHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA