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The map label for this gene is lysS [H]

Identifier: 159041752

GI number: 159041752

Start: 1245381

End: 1246862

Strand: Reverse

Name: lysS [H]

Synonym: Cmaq_1187

Alternate gene names: 159041752

Gene position: 1246862-1245381 (Counterclockwise)

Preceding gene: 159041761

Following gene: 159041749

Centisome position: 60.02

GC content: 44.74

Gene sequence:

>1482_bases
ATGTATAAGCCTCAATGGGACCCGAATAGGTTAAGGATCGTGGAGGAGTTGAGGAGTAGGGGTGTTAACCCGTATCCACA
TAAATTTGAGATTACCCACACGATAAGTCAGATTAGGGAGCTAGCTAAGAAGTACCCTATTAGCCATGATCCATTCCTTA
AGGGTATTAGGACGGTGGGTAGGGTGGCTAACGTTAGGAGGCATGGTAAGGCTTCATTCGTTGATATTTTCGATGAGGGT
GAGAGGCTTCAACTCTACTTGAGGGTTAATGAGCTTGGGGATAGGTTTGAGGAGTTTCATAGATATGTGGATAGGGGTGA
TGTAATTGGGGTTGAGGGTGACTTATTCTACACGCTTAAGGGTGAGTTAACGCTACTGGTTCATGATTACGCATTATTGG
CTAAGGCGCTCCTTGAGCCACCTGACTGGAGTAAATACACCACTGAGTGGAGGTATACGCATAGGTACCTTGACTTCCTC
TATAATAGTGAGGCTAGGAGGATTATTCAAACCAGGTTCAGGATTATTCAAGCCATTAGGGAGTTCTTGAATTCAATGGG
GTTCATGGAGGTTGAAACCCCAATACTTCAACCAGTCTACGGTGGTGCCTTGGCTAAGCCCTTTAAGACTCATGTTAATG
CCCTTGATGAGGACTGGTACCTTAGGATTAGCCTTGAACTTTACTTGAAGAGGTTCATAATTGGGGGTTTCAATAAGGTT
TACGAGATTGGTAAAGTCTTCAGGAATGAGGACATTGATGTTTACCATAACCCGGAATTCACAATGCTTGAATTATACTG
GGCCTACGCGGACTACAATGATATCATGAGGCTTACGGAGGATTTGATTCATCAAGTGGCAGTGAAGGTTACTGGTAAGG
ACATGGTGGAGTACCCTATAGGTGAAGGTAAGGTTGAGGTTAAGTTAACTACACCGTTCAGGAGACTAACAATGTATGAG
GCCTTAAGTAATGAGCTGGGTAAGAACGTTGAGTCAATGAGTGATGATGAGTTGAGGAGATTAATGAATGAGAATGGGCT
GGTACCTAGGGGTGGGCAGTACGTTAGGGGGCTTATGATTGAGAAGCTATTTGATAAATTAGTGGCACCTAAGTTAATTC
AACCAACCTTCATTACTGATTACCCACAGGAGACTACACCATTATGTAAGCCACATAGGTCTAAGCCAGGCTTAATAGAG
AGGTTTGAATTATACATTGCCGGAATGGAGCTGGCCAACGCCTACACTGAGCTTAATGATCCCCAGCTACAGCACAAGTT
CTTCGAGGAGGAGGCTGAACGCTTCAAGGGAGGTGACGAGGAGGCGCATCCCTACGACTACGACTTCGTCCTGGCCCTAA
GCTACGGAATGCCACCAACAGGTGGATTAGGCATAGGCATTGATAGGCTAGTAATGGTGCTGACTGGGCAATACTCCATT
AAGGAAGTAATACCATTCCCAATGGTAAAACATAATCCTTAA

Upstream 100 bases:

>100_bases
CAGACACACTATACTTCACTGCGCTTTTAGGGCTTTACCCATGGGTTGCATGTGGTACAATGTTTATTAAGACTATCCGC
ATTGAACTTAACGCATCAGT

Downstream 100 bases:

>100_bases
TCATTAAGCCATCCAACCTCATCACCTGCATTAACATTTTCATTGATCATTTTATTAATATGCTAATGTCGAATTTAATG
CGTATGAACCCATAATTAAT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MYKPQWDPNRLRIVEELRSRGVNPYPHKFEITHTISQIRELAKKYPISHDPFLKGIRTVGRVANVRRHGKASFVDIFDEG
ERLQLYLRVNELGDRFEEFHRYVDRGDVIGVEGDLFYTLKGELTLLVHDYALLAKALLEPPDWSKYTTEWRYTHRYLDFL
YNSEARRIIQTRFRIIQAIREFLNSMGFMEVETPILQPVYGGALAKPFKTHVNALDEDWYLRISLELYLKRFIIGGFNKV
YEIGKVFRNEDIDVYHNPEFTMLELYWAYADYNDIMRLTEDLIHQVAVKVTGKDMVEYPIGEGKVEVKLTTPFRRLTMYE
ALSNELGKNVESMSDDELRRLMNENGLVPRGGQYVRGLMIEKLFDKLVAPKLIQPTFITDYPQETTPLCKPHRSKPGLIE
RFELYIAGMELANAYTELNDPQLQHKFFEEEAERFKGGDEEAHPYDYDFVLALSYGMPPTGGLGIGIDRLVMVLTGQYSI
KEVIPFPMVKHNP

Sequences:

>Translated_493_residues
MYKPQWDPNRLRIVEELRSRGVNPYPHKFEITHTISQIRELAKKYPISHDPFLKGIRTVGRVANVRRHGKASFVDIFDEG
ERLQLYLRVNELGDRFEEFHRYVDRGDVIGVEGDLFYTLKGELTLLVHDYALLAKALLEPPDWSKYTTEWRYTHRYLDFL
YNSEARRIIQTRFRIIQAIREFLNSMGFMEVETPILQPVYGGALAKPFKTHVNALDEDWYLRISLELYLKRFIIGGFNKV
YEIGKVFRNEDIDVYHNPEFTMLELYWAYADYNDIMRLTEDLIHQVAVKVTGKDMVEYPIGEGKVEVKLTTPFRRLTMYE
ALSNELGKNVESMSDDELRRLMNENGLVPRGGQYVRGLMIEKLFDKLVAPKLIQPTFITDYPQETTPLCKPHRSKPGLIE
RFELYIAGMELANAYTELNDPQLQHKFFEEEAERFKGGDEEAHPYDYDFVLALSYGMPPTGGLGIGIDRLVMVLTGQYSI
KEVIPFPMVKHNP
>Mature_493_residues
MYKPQWDPNRLRIVEELRSRGVNPYPHKFEITHTISQIRELAKKYPISHDPFLKGIRTVGRVANVRRHGKASFVDIFDEG
ERLQLYLRVNELGDRFEEFHRYVDRGDVIGVEGDLFYTLKGELTLLVHDYALLAKALLEPPDWSKYTTEWRYTHRYLDFL
YNSEARRIIQTRFRIIQAIREFLNSMGFMEVETPILQPVYGGALAKPFKTHVNALDEDWYLRISLELYLKRFIIGGFNKV
YEIGKVFRNEDIDVYHNPEFTMLELYWAYADYNDIMRLTEDLIHQVAVKVTGKDMVEYPIGEGKVEVKLTTPFRRLTMYE
ALSNELGKNVESMSDDELRRLMNENGLVPRGGQYVRGLMIEKLFDKLVAPKLIQPTFITDYPQETTPLCKPHRSKPGLIE
RFELYIAGMELANAYTELNDPQLQHKFFEEEAERFKGGDEEAHPYDYDFVLALSYGMPPTGGLGIGIDRLVMVLTGQYSI
KEVIPFPMVKHNP

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=505, Percent_Identity=41.1881188118812, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI194272210, Length=505, Percent_Identity=41.1881188118812, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI45439306, Length=346, Percent_Identity=26.0115606936416, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1790571, Length=488, Percent_Identity=41.8032786885246, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789256, Length=480, Percent_Identity=41.6666666666667, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=30.1242236024845, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788173, Length=184, Percent_Identity=27.7173913043478, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=501, Percent_Identity=40.5189620758483, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI71994340, Length=501, Percent_Identity=40.5189620758483, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI17535925, Length=501, Percent_Identity=40.5189620758483, Blast_Score=357, Evalue=7e-99,
Organism=Saccharomyces cerevisiae, GI6320242, Length=518, Percent_Identity=39.5752895752896, Blast_Score=328, Evalue=9e-91,
Organism=Saccharomyces cerevisiae, GI6324256, Length=470, Percent_Identity=33.1914893617021, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6325153, Length=152, Percent_Identity=29.6052631578947, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640849, Length=500, Percent_Identity=40, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24640851, Length=500, Percent_Identity=40, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24584738, Length=174, Percent_Identity=30.4597701149425, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57575; Mature: 57575

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKPQWDPNRLRIVEELRSRGVNPYPHKFEITHTISQIRELAKKYPISHDPFLKGIRTVG
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RVANVRRHGKASFVDIFDEGERLQLYLRVNELGDRFEEFHRYVDRGDVIGVEGDLFYTLK
HHHHHHHCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC
GELTLLVHDYALLAKALLEPPDWSKYTTEWRYTHRYLDFLYNSEARRIIQTRFRIIQAIR
CCEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
EFLNSMGFMEVETPILQPVYGGALAKPFKTHVNALDEDWYLRISLELYLKRFIIGGFNKV
HHHHHCCCEEECCHHHHHHHCCHHCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCHHHH
YEIGKVFRNEDIDVYHNPEFTMLELYWAYADYNDIMRLTEDLIHQVAVKVTGKDMVEYPI
HHHHHHHCCCCCCEEECCCEEEEEEEEHHHCHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
GEGKVEVKLTTPFRRLTMYEALSNELGKNVESMSDDELRRLMNENGLVPRGGQYVRGLMI
CCCEEEEEEECCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
EKLFDKLVAPKLIQPTFITDYPQETTPLCKPHRSKPGLIERFELYIAGMELANAYTELND
HHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PQLQHKFFEEEAERFKGGDEEAHPYDYDFVLALSYGMPPTGGLGIGIDRLVMVLTGQYSI
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHH
KEVIPFPMVKHNP
HHHCCCCCCCCCC
>Mature Secondary Structure
MYKPQWDPNRLRIVEELRSRGVNPYPHKFEITHTISQIRELAKKYPISHDPFLKGIRTVG
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RVANVRRHGKASFVDIFDEGERLQLYLRVNELGDRFEEFHRYVDRGDVIGVEGDLFYTLK
HHHHHHHCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC
GELTLLVHDYALLAKALLEPPDWSKYTTEWRYTHRYLDFLYNSEARRIIQTRFRIIQAIR
CCEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
EFLNSMGFMEVETPILQPVYGGALAKPFKTHVNALDEDWYLRISLELYLKRFIIGGFNKV
HHHHHCCCEEECCHHHHHHHCCHHCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCHHHH
YEIGKVFRNEDIDVYHNPEFTMLELYWAYADYNDIMRLTEDLIHQVAVKVTGKDMVEYPI
HHHHHHHCCCCCCEEECCCEEEEEEEEHHHCHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
GEGKVEVKLTTPFRRLTMYEALSNELGKNVESMSDDELRRLMNENGLVPRGGQYVRGLMI
CCCEEEEEEECCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
EKLFDKLVAPKLIQPTFITDYPQETTPLCKPHRSKPGLIERFELYIAGMELANAYTELND
HHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PQLQHKFFEEEAERFKGGDEEAHPYDYDFVLALSYGMPPTGGLGIGIDRLVMVLTGQYSI
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHH
KEVIPFPMVKHNP
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]