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The map label for this gene is prpB [H]

Identifier: 159041356

GI number: 159041356

Start: 817905

End: 818843

Strand: Reverse

Name: prpB [H]

Synonym: Cmaq_0783

Alternate gene names: 159041356

Gene position: 818843-817905 (Counterclockwise)

Preceding gene: 159041359

Following gene: 159041355

Centisome position: 39.41

GC content: 43.88

Gene sequence:

>939_bases
ATGAAACCTGTACTACTTGATAAGGGTAAGCCTAAGCCAAGTGAGATTAGGGGGAGGTTTAGGGAGAGACTGGGTAAGGA
TGGTATAATAGTGGCTCCTGGTGTTTTCATTCCAGCTGCAGCAATGTTAGCTGAGGAATTGGGGTTTGAGGCTGTTTACT
TCAGTGGCGCCGCGTACTCCAATATGCTTGGTTTACCTGATCTAGGCGTCTTCACGCTTAGTGAATTATCATACTACCTG
AGGTTCTTTACAAATGCAGTAAGCATACCCCTTATCGTTGATATTGACACAGGCTTTGGGGAGGCATTAAATGTTGCAAG
GACCATTAGGGAGCTTGAGGATGTTGGCGTATCCGCAGTTCATATTGAGGATCAGGAAATGCCTAAGAAGTGCGGGCACT
TAGCAGGTAAGAGGGTTATACCCATTGATGAAATGGTTAAGAAGATTAAGGCTGCAGTTGAGGCTAGGAGGGATGATAAC
TTCATTATAATAGCTAGGACTGATGCTAGGGATGTTGAGGGCTTTGATTCAGCAGTGGAGAGGGCTAAGGTTTACCTTGA
AGCTGGCGCTGATGCAATATTCCCTGAGGCACTCCACAGTAAGGAGGAGTTCATGGAGTTCGCCAGGAGGGTTAAGGCAC
CACTGCTTGCTAATATGACTGAGTTTGGAAAGACACCGTACATTACTGCGAAGGAGTTTGAGGAAATGGGTTATAAGATA
GTTATATTCCCTGTAACAACATTCAGGTACGCTATGGGTGCTATTAAAAGGGCGTTAATTACCCTTAAGAATGAGGGTAG
TCAATTAAGCTTAATTAACGATATGATGAGTAGGGAGGATGTTTACCAACTCATTGGATACCATGATTATGAGGAGTGGG
ATAAGAATTTAGCTGAGGAGGCTAGTAAACTATTAAGTGGTAGAATTAAGCTGAGGTAG

Upstream 100 bases:

>100_bases
CTTAAAGCCCTCTAAGAACCTGAGGGTCTGGTTTGCATATTCTTCACTTCCCTGAATCCTAACGTGAAACTACTTTAATC
CCTTAATCTAGGCTTAAATC

Downstream 100 bases:

>100_bases
AGTTAGGATTACGCAACTAGGCGTAATGAATTAGTACTTAAATGAGGCTCATGAGTAATTATGATCCTACCTTGACCTAG
GGAGATAAGTTATATGATAA

Product: methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKPVLLDKGKPKPSEIRGRFRERLGKDGIIVAPGVFIPAAAMLAEELGFEAVYFSGAAYSNMLGLPDLGVFTLSELSYYL
RFFTNAVSIPLIVDIDTGFGEALNVARTIRELEDVGVSAVHIEDQEMPKKCGHLAGKRVIPIDEMVKKIKAAVEARRDDN
FIIIARTDARDVEGFDSAVERAKVYLEAGADAIFPEALHSKEEFMEFARRVKAPLLANMTEFGKTPYITAKEFEEMGYKI
VIFPVTTFRYAMGAIKRALITLKNEGSQLSLINDMMSREDVYQLIGYHDYEEWDKNLAEEASKLLSGRIKLR

Sequences:

>Translated_312_residues
MKPVLLDKGKPKPSEIRGRFRERLGKDGIIVAPGVFIPAAAMLAEELGFEAVYFSGAAYSNMLGLPDLGVFTLSELSYYL
RFFTNAVSIPLIVDIDTGFGEALNVARTIRELEDVGVSAVHIEDQEMPKKCGHLAGKRVIPIDEMVKKIKAAVEARRDDN
FIIIARTDARDVEGFDSAVERAKVYLEAGADAIFPEALHSKEEFMEFARRVKAPLLANMTEFGKTPYITAKEFEEMGYKI
VIFPVTTFRYAMGAIKRALITLKNEGSQLSLINDMMSREDVYQLIGYHDYEEWDKNLAEEASKLLSGRIKLR
>Mature_312_residues
MKPVLLDKGKPKPSEIRGRFRERLGKDGIIVAPGVFIPAAAMLAEELGFEAVYFSGAAYSNMLGLPDLGVFTLSELSYYL
RFFTNAVSIPLIVDIDTGFGEALNVARTIRELEDVGVSAVHIEDQEMPKKCGHLAGKRVIPIDEMVKKIKAAVEARRDDN
FIIIARTDARDVEGFDSAVERAKVYLEAGADAIFPEALHSKEEFMEFARRVKAPLLANMTEFGKTPYITAKEFEEMGYKI
VIFPVTTFRYAMGAIKRALITLKNEGSQLSLINDMMSREDVYQLIGYHDYEEWDKNLAEEASKLLSGRIKLR

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=280, Percent_Identity=42.1428571428571, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1790445, Length=189, Percent_Identity=28.042328042328, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 34943; Mature: 34943

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVLLDKGKPKPSEIRGRFRERLGKDGIIVAPGVFIPAAAMLAEELGFEAVYFSGAAYS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCEEEEECCCHHH
NMLGLPDLGVFTLSELSYYLRFFTNAVSIPLIVDIDTGFGEALNVARTIRELEDVGVSAV
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
HIEDQEMPKKCGHLAGKRVIPIDEMVKKIKAAVEARRDDNFIIIARTDARDVEGFDSAVE
EECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
RAKVYLEAGADAIFPEALHSKEEFMEFARRVKAPLLANMTEFGKTPYITAKEFEEMGYKI
HHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCCEE
VIFPVTTFRYAMGAIKRALITLKNEGSQLSLINDMMSREDVYQLIGYHDYEEWDKNLAEE
EEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ASKLLSGRIKLR
HHHHHHCCCCCC
>Mature Secondary Structure
MKPVLLDKGKPKPSEIRGRFRERLGKDGIIVAPGVFIPAAAMLAEELGFEAVYFSGAAYS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCEEEEECCCHHH
NMLGLPDLGVFTLSELSYYLRFFTNAVSIPLIVDIDTGFGEALNVARTIRELEDVGVSAV
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEE
HIEDQEMPKKCGHLAGKRVIPIDEMVKKIKAAVEARRDDNFIIIARTDARDVEGFDSAVE
EECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
RAKVYLEAGADAIFPEALHSKEEFMEFARRVKAPLLANMTEFGKTPYITAKEFEEMGYKI
HHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCCEE
VIFPVTTFRYAMGAIKRALITLKNEGSQLSLINDMMSREDVYQLIGYHDYEEWDKNLAEE
EEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ASKLLSGRIKLR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]