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The map label for this gene is purD [H]

Identifier: 159041241

GI number: 159041241

Start: 697974

End: 699446

Strand: Reverse

Name: purD [H]

Synonym: Cmaq_0663

Alternate gene names: 159041241

Gene position: 699446-697974 (Counterclockwise)

Preceding gene: 159041243

Following gene: 159041240

Centisome position: 33.67

GC content: 48.4

Gene sequence:

>1473_bases
ATGATAAAAAGCAAAGTACTGTTGGTTGGGGATGGAGCAAGGGAACACGCTATTGCTGAAGCCTTATCAAGAAGTGTAAA
TGAACCAAGAATCAGCGCCCTGGTTAACCATATTAACCCTGGGATAAGGAGAATTGTCGAATCCACCGGTGGCTCACTGG
TAATTGGACCACTTAACCAGGAGGGGGTTGTTAAGGCCATTAATGTGATTAACCCTGACTTAGTGGTAATTGGACCCGAG
GAACCACAGTTCGCTGGTGTTGCTGATAAGGCTGTGGAAATGGGTGTACCAACCTTCGGTGCATTAAGTAGGTTAGCTAG
GATTGAGCAGAGTAAGGCCTTCGCCAGGTGGTTGATGTGGAGGTATAGGATCCCAGGTAGGATAGCCTATAGGGCGTTTA
GAAGCGTGGAGGAGGCTATTGAGTACGTTAAGAACGCAGGCTCAGTGGCTATTAAGCCGGCTAGGCAGAGTGGTGGTAAG
GGTGTTAGGGTCTTCTGGGTTAACCAAGCTTACTTAAGGGATGGAGTTAATGACGCTAAGGAGACTCAATTAATTGATGC
TGCTAATGATGTGCTTAAGTACGGTGACGTAGAAGACTTAATTATTGTTGAGGAGACTGTAAGTGGTGTCGAGTACACTG
TTCAAGTGATAACCGATGGAGTAAACATTCTTCCCCTACCACCCATTCAAGATCACCCACATGCCTTTGATGGCGACATT
GGACCTGAATGCGGAGGCATGGGTAGTGTGGCTGGTCCAGGAGCTAGATTACCCTTCCTAAGGGATGATGAGTACTGGGA
GAGCGTTAATATTGTTAAGAGCACTATCAATGCCCTACAGAGGGAGACTGGGTTAAGGTACGTGGGTGTGCTTTCAGGTC
AATTCATGTTAACATCCTATGGGCCAACACTCATAGAGTACTACAGTAGGTTAGGTGACCCTGAGGCCTTAAATGCACTA
GCCCTATTGAGGGGGGATTTCCTTGAATTAATTGAAGCAGCAGTGGAGGGGAGGTTACCATCATTCAATTACTCCTTCAG
CAACGAGGTCACTGTGGCCAAGGCGATAGCACCCCTAGGTTACCCGCATAGAAGAGACTTAGCCGCTGGGAGGAGGATTA
GCATTGACTTCAATGGCATTAAGAAACTGGGGTGTGGGCTTTACTTCGGCTCAGTGGAGTACGTTAATGGCGAGTATAGG
ACGCTGGGCTCTAGGGCCGTTGAAATACTGGCCACTGGGTCAAGTTACAGTGAGGCTCATGCTAAGGCTGAGGAATGCAT
CTCACTGGTTAAGTCCAGTGACTGGAAGCTGATACATAGGGTTGATGTGGGTGAACCAAGCCTCATGGAGAGGAGGATTA
GGGACGCTGAATTAGTCAGGGAGGTTTACAGGTGGAGGAGGAGCCATGGATTGGGGAGGGTTAGGATTGACTGGGTGCCT
GGCAGGGATGTTGCAGTGTATGATTACTCGTAA

Upstream 100 bases:

>100_bases
TTATGGTAAATAATTCAACATAAAACCTCATTACCATTAAATAACATACCTTAATAATTTAAAAATTAAATTTAAAAATA
GGTAACTTATTTTATTATTA

Downstream 100 bases:

>100_bases
TTAAATTAATTAACTTAATAAGATAATTAATTATCGGAAAAGTTTAAATATTCTTTTAATCAATTAATATTATGGAGGCG
GGAAACACAATTAAACCCAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MIKSKVLLVGDGAREHAIAEALSRSVNEPRISALVNHINPGIRRIVESTGGSLVIGPLNQEGVVKAINVINPDLVVIGPE
EPQFAGVADKAVEMGVPTFGALSRLARIEQSKAFARWLMWRYRIPGRIAYRAFRSVEEAIEYVKNAGSVAIKPARQSGGK
GVRVFWVNQAYLRDGVNDAKETQLIDAANDVLKYGDVEDLIIVEETVSGVEYTVQVITDGVNILPLPPIQDHPHAFDGDI
GPECGGMGSVAGPGARLPFLRDDEYWESVNIVKSTINALQRETGLRYVGVLSGQFMLTSYGPTLIEYYSRLGDPEALNAL
ALLRGDFLELIEAAVEGRLPSFNYSFSNEVTVAKAIAPLGYPHRRDLAAGRRISIDFNGIKKLGCGLYFGSVEYVNGEYR
TLGSRAVEILATGSSYSEAHAKAEECISLVKSSDWKLIHRVDVGEPSLMERRIRDAELVREVYRWRRSHGLGRVRIDWVP
GRDVAVYDYS

Sequences:

>Translated_490_residues
MIKSKVLLVGDGAREHAIAEALSRSVNEPRISALVNHINPGIRRIVESTGGSLVIGPLNQEGVVKAINVINPDLVVIGPE
EPQFAGVADKAVEMGVPTFGALSRLARIEQSKAFARWLMWRYRIPGRIAYRAFRSVEEAIEYVKNAGSVAIKPARQSGGK
GVRVFWVNQAYLRDGVNDAKETQLIDAANDVLKYGDVEDLIIVEETVSGVEYTVQVITDGVNILPLPPIQDHPHAFDGDI
GPECGGMGSVAGPGARLPFLRDDEYWESVNIVKSTINALQRETGLRYVGVLSGQFMLTSYGPTLIEYYSRLGDPEALNAL
ALLRGDFLELIEAAVEGRLPSFNYSFSNEVTVAKAIAPLGYPHRRDLAAGRRISIDFNGIKKLGCGLYFGSVEYVNGEYR
TLGSRAVEILATGSSYSEAHAKAEECISLVKSSDWKLIHRVDVGEPSLMERRIRDAELVREVYRWRRSHGLGRVRIDWVP
GRDVAVYDYS
>Mature_490_residues
MIKSKVLLVGDGAREHAIAEALSRSVNEPRISALVNHINPGIRRIVESTGGSLVIGPLNQEGVVKAINVINPDLVVIGPE
EPQFAGVADKAVEMGVPTFGALSRLARIEQSKAFARWLMWRYRIPGRIAYRAFRSVEEAIEYVKNAGSVAIKPARQSGGK
GVRVFWVNQAYLRDGVNDAKETQLIDAANDVLKYGDVEDLIIVEETVSGVEYTVQVITDGVNILPLPPIQDHPHAFDGDI
GPECGGMGSVAGPGARLPFLRDDEYWESVNIVKSTINALQRETGLRYVGVLSGQFMLTSYGPTLIEYYSRLGDPEALNAL
ALLRGDFLELIEAAVEGRLPSFNYSFSNEVTVAKAIAPLGYPHRRDLAAGRRISIDFNGIKKLGCGLYFGSVEYVNGEYR
TLGSRAVEILATGSSYSEAHAKAEECISLVKSSDWKLIHRVDVGEPSLMERRIRDAELVREVYRWRRSHGLGRVRIDWVP
GRDVAVYDYS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=450, Percent_Identity=28, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI4503915, Length=450, Percent_Identity=28.2222222222222, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI209869995, Length=450, Percent_Identity=28.2222222222222, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI209869993, Length=450, Percent_Identity=28.2222222222222, Blast_Score=167, Evalue=3e-41,
Organism=Escherichia coli, GI1790438, Length=450, Percent_Identity=28.6666666666667, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17567511, Length=465, Percent_Identity=27.5268817204301, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321203, Length=402, Percent_Identity=29.6019900497512, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24582400, Length=457, Percent_Identity=30.4157549234136, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI62471619, Length=457, Percent_Identity=30.4157549234136, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 53910; Mature: 53910

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50975 ATP_GRASP ; PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSKVLLVGDGAREHAIAEALSRSVNEPRISALVNHINPGIRRIVESTGGSLVIGPLNQ
CCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCEEEEECCCC
EGVVKAINVINPDLVVIGPEEPQFAGVADKAVEMGVPTFGALSRLARIEQSKAFARWLMW
CCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RYRIPGRIAYRAFRSVEEAIEYVKNAGSVAIKPARQSGGKGVRVFWVNQAYLRDGVNDAK
HHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECHHHHHCCCCHHH
ETQLIDAANDVLKYGDVEDLIIVEETVSGVEYTVQVITDGVNILPLPPIQDHPHAFDGDI
HHHHHHHHHHHHHCCCCCCEEEEECHHCCHHEEEEEEECCCCEEECCCCCCCCCCCCCCC
GPECGGMGSVAGPGARLPFLRDDEYWESVNIVKSTINALQRETGLRYVGVLSGQFMLTSY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECC
GPTLIEYYSRLGDPEALNALALLRGDFLELIEAAVEGRLPSFNYSFSNEVTVAKAIAPLG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCC
YPHRRDLAAGRRISIDFNGIKKLGCGLYFGSVEYVNGEYRTLGSRAVEILATGSSYSEAH
CCCCHHHCCCCEEEEECCCHHHHCCEEEECCEEEECCHHHHHHHHEEEEEECCCCHHHHH
AKAEECISLVKSSDWKLIHRVDVGEPSLMERRIRDAELVREVYRWRRSHGLGRVRIDWVP
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GRDVAVYDYS
CCCEEEEECC
>Mature Secondary Structure
MIKSKVLLVGDGAREHAIAEALSRSVNEPRISALVNHINPGIRRIVESTGGSLVIGPLNQ
CCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHCCCEEEEECCCC
EGVVKAINVINPDLVVIGPEEPQFAGVADKAVEMGVPTFGALSRLARIEQSKAFARWLMW
CCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RYRIPGRIAYRAFRSVEEAIEYVKNAGSVAIKPARQSGGKGVRVFWVNQAYLRDGVNDAK
HHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECHHHHHCCCCHHH
ETQLIDAANDVLKYGDVEDLIIVEETVSGVEYTVQVITDGVNILPLPPIQDHPHAFDGDI
HHHHHHHHHHHHHCCCCCCEEEEECHHCCHHEEEEEEECCCCEEECCCCCCCCCCCCCCC
GPECGGMGSVAGPGARLPFLRDDEYWESVNIVKSTINALQRETGLRYVGVLSGQFMLTSY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECC
GPTLIEYYSRLGDPEALNALALLRGDFLELIEAAVEGRLPSFNYSFSNEVTVAKAIAPLG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCC
YPHRRDLAAGRRISIDFNGIKKLGCGLYFGSVEYVNGEYRTLGSRAVEILATGSSYSEAH
CCCCHHHCCCCEEEEECCCHHHHCCEEEECCEEEECCHHHHHHHHEEEEEECCCCHHHHH
AKAEECISLVKSSDWKLIHRVDVGEPSLMERRIRDAELVREVYRWRRSHGLGRVRIDWVP
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GRDVAVYDYS
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]