Click here to switch to the map view.

The map label for this gene is aegA [H]

Identifier: 159041191

GI number: 159041191

Start: 645608

End: 646684

Strand: Reverse

Name: aegA [H]

Synonym: Cmaq_0612

Alternate gene names: 159041191

Gene position: 646684-645608 (Counterclockwise)

Preceding gene: 159041192

Following gene: 159041189

Centisome position: 31.13

GC content: 47.17

Gene sequence:

>1077_bases
ATGAAGTTTGCATTGAAGTGTAGTCCAGGTCAGAAGATCAGTAAGAGGCAGGGGAAGGTGGCTATAATTGGGGCTGGGCC
GGCGGGCCTGGGTGCGGCTGGTTACTTAATATGCAAGGGGTATGATGTGGATGTCTACGATAGGATGCCTGAACCAGGTG
GAATGACGCTCTTCGCAATACCCAATTGGAGACTACCTTGGAATAACATTAGGGCTGGGGTGAGGGAACTTAGGGAACTG
GGTGTTAACTTCTTCAGTAATGTTAAGGTTATTTGCGATGAAGAGTACAGTATTGAGGGTGATCAGTTCGTTAAAAGTAA
GATTCACTTGGAGGAGTTGATTGATAAATACGACGCCTTAGTAATAGCCACAGGGACTTGGGAATCCAGGAGGCTTAAAA
TCCCTGGGGAGAATCTTAAAGGATCATACCTAGCCCTCGATTACTTATTCAGAATATACGCAAGCCAACATGGTTACTTA
CCTAAATCTGAAGTTTTTAAAACAGGCCTAAAGTCCATGGTGGTTGGGGCTGGTTTAACCGCTGTTGATGCTGCAATAGA
GTCTCAATTAGAGGGGGCTAAGGAGGTTTACTTATCCTACAGGAGGACCATAAACGAGGCCCCTGCCGGTAAGGCTGAGA
TAAATAGGTTAATTCAAAGGGGGGTTAAGCTCCTTGAATTAACACTACCGGTGGAGGTTAATGGTAAGGAGTACGTTGAG
GCTGTTAAGATGATTAAAATGAGGCTAGGTAAGCCGGATGCCTCAGGTAGACCTGCCCCAGAGCCAATACCTGGTTCTGA
ATTCATACTCAACGTAGATACCTTAATATCAGCGGTTGGTGAAATACCAACACCACCCATGAGGAAGGAGTGCTGCGGTG
TGAAGTTAAGGCAAAACGGCACCATAGATACTGATGCCCAAGGTAGAACAACCAGGCTCGGTGTCTTTGCGGCTGGCGAC
GTAACAAATGGCCCGACTTTAATTGGTAAGGCATTGTCCCACGGCATGAGGATAGCGCAATCCGTTGAGGCATTCCTTGA
GGCTGGGGGTAAGAAGGGGAGTAGTTGGAGATCCTAG

Upstream 100 bases:

>100_bases
GGAACAGGGATGAGGTTTCATCTAGGTATATAAAATGAACATAATACTGAATTAAAGGTCGTGTGAAAAACATTAAAAAT
AGTGAAACAGAGCCTAATTC

Downstream 100 bases:

>100_bases
GCTTCACCGTAGGCCCTATCCCCAGCGTCCCCAAGCCCAGGTACAATGAATCCCCTCTCATTCAACTCAGGGTCAACGGC
AACTAGGTAAACGTCAATAT

Product: putative glutamate synthase subunit beta

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKFALKCSPGQKISKRQGKVAIIGAGPAGLGAAGYLICKGYDVDVYDRMPEPGGMTLFAIPNWRLPWNNIRAGVRELREL
GVNFFSNVKVICDEEYSIEGDQFVKSKIHLEELIDKYDALVIATGTWESRRLKIPGENLKGSYLALDYLFRIYASQHGYL
PKSEVFKTGLKSMVVGAGLTAVDAAIESQLEGAKEVYLSYRRTINEAPAGKAEINRLIQRGVKLLELTLPVEVNGKEYVE
AVKMIKMRLGKPDASGRPAPEPIPGSEFILNVDTLISAVGEIPTPPMRKECCGVKLRQNGTIDTDAQGRTTRLGVFAAGD
VTNGPTLIGKALSHGMRIAQSVEAFLEAGGKKGSSWRS

Sequences:

>Translated_358_residues
MKFALKCSPGQKISKRQGKVAIIGAGPAGLGAAGYLICKGYDVDVYDRMPEPGGMTLFAIPNWRLPWNNIRAGVRELREL
GVNFFSNVKVICDEEYSIEGDQFVKSKIHLEELIDKYDALVIATGTWESRRLKIPGENLKGSYLALDYLFRIYASQHGYL
PKSEVFKTGLKSMVVGAGLTAVDAAIESQLEGAKEVYLSYRRTINEAPAGKAEINRLIQRGVKLLELTLPVEVNGKEYVE
AVKMIKMRLGKPDASGRPAPEPIPGSEFILNVDTLISAVGEIPTPPMRKECCGVKLRQNGTIDTDAQGRTTRLGVFAAGD
VTNGPTLIGKALSHGMRIAQSVEAFLEAGGKKGSSWRS
>Mature_358_residues
MKFALKCSPGQKISKRQGKVAIIGAGPAGLGAAGYLICKGYDVDVYDRMPEPGGMTLFAIPNWRLPWNNIRAGVRELREL
GVNFFSNVKVICDEEYSIEGDQFVKSKIHLEELIDKYDALVIATGTWESRRLKIPGENLKGSYLALDYLFRIYASQHGYL
PKSEVFKTGLKSMVVGAGLTAVDAAIESQLEGAKEVYLSYRRTINEAPAGKAEINRLIQRGVKLLELTLPVEVNGKEYVE
AVKMIKMRLGKPDASGRPAPEPIPGSEFILNVDTLISAVGEIPTPPMRKECCGVKLRQNGTIDTDAQGRTTRLGVFAAGD
VTNGPTLIGKALSHGMRIAQSVEAFLEAGGKKGSSWRS

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=349, Percent_Identity=23.2091690544413, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788811, Length=345, Percent_Identity=33.9130434782609, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1789606, Length=355, Percent_Identity=32.112676056338, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082180, Length=346, Percent_Identity=31.5028901734104, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788468, Length=336, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789243, Length=326, Percent_Identity=31.2883435582822, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17570289, Length=195, Percent_Identity=30.2564102564103, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=370, Percent_Identity=28.1081081081081, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24665547, Length=353, Percent_Identity=26.9121813031161, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24665543, Length=353, Percent_Identity=26.9121813031161, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574881, Length=353, Percent_Identity=26.9121813031161, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24665539, Length=353, Percent_Identity=26.9121813031161, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 38975; Mature: 38975

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFALKCSPGQKISKRQGKVAIIGAGPAGLGAAGYLICKGYDVDVYDRMPEPGGMTLFAI
CCEEEECCCCCHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEEC
PNWRLPWNNIRAGVRELRELGVNFFSNVKVICDEEYSIEGDQFVKSKIHLEELIDKYDAL
CCCCCCHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCEE
VIATGTWESRRLKIPGENLKGSYLALDYLFRIYASQHGYLPKSEVFKTGLKSMVVGAGLT
EEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHH
AVDAAIESQLEGAKEVYLSYRRTINEAPAGKAEINRLIQRGVKLLELTLPVEVNGKEYVE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHEEEEEECEEECCHHHHH
AVKMIKMRLGKPDASGRPAPEPIPGSEFILNVDTLISAVGEIPTPPMRKECCGVKLRQNG
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCHHHHCCCEEECCC
TIDTDAQGRTTRLGVFAAGDVTNGPTLIGKALSHGMRIAQSVEAFLEAGGKKGSSWRS
CCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFALKCSPGQKISKRQGKVAIIGAGPAGLGAAGYLICKGYDVDVYDRMPEPGGMTLFAI
CCEEEECCCCCHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEEC
PNWRLPWNNIRAGVRELRELGVNFFSNVKVICDEEYSIEGDQFVKSKIHLEELIDKYDAL
CCCCCCHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCEE
VIATGTWESRRLKIPGENLKGSYLALDYLFRIYASQHGYLPKSEVFKTGLKSMVVGAGLT
EEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHH
AVDAAIESQLEGAKEVYLSYRRTINEAPAGKAEINRLIQRGVKLLELTLPVEVNGKEYVE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHEEEEEECEEECCHHHHH
AVKMIKMRLGKPDASGRPAPEPIPGSEFILNVDTLISAVGEIPTPPMRKECCGVKLRQNG
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCHHHHCCCEEECCC
TIDTDAQGRTTRLGVFAAGDVTNGPTLIGKALSHGMRIAQSVEAFLEAGGKKGSSWRS
CCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]